A microscope is the fundamental research and diagnostic apparatus for clinical investigation of signaling transduction, morphological changes and physiological tracking of cells and intact tissues from patients in the biomedical laboratory science. Proper use, care and maintenance of microscope with comprehensive understanding in mechanism are fully requested for reliable image data and accurate interpretation for diagnosis in the clinical laboratory. The standard operating procedure (SOP) for light microscopes includes performance procedure, brief information of all mechanical parts of microscopes with systematic troubleshooting mechanism depending on the laboratory capacity. Maintenance program encompasses cleaning objective, ocular lenses and inner optics; replacement and calibration of light source; XY sample stage management; point spread function (PSF) measurement for confocal laser scanning microscope (CLSM); quality control (QC) program in fluorescent microscopy; and systematic troubleshooting. Laser safety is one of the concern for medical technologists engaged in CLSM laboratory. Laser safety guideline based on the laser classification and risk level, and advisory lab wear for CLSM users are also expatiated in this overview. Since acquired image data presents a wide range of information at the moment of acquisition, well-maintained microscopes with proper microscopic maintenance program are impulsive for its interpretation and diagnosis in the clinical laboratory.
Journal of the Korea Academia-Industrial cooperation Society
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v.22
no.2
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pp.392-400
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2021
Basic nursing core skills are the essential skills required of nurses to effectively care for their patients. This study introduces an on-campus practicum using a mobile-based reflective journal, and attempts identify the challenges faced by students when performing core clinical nursing skills. The on-campus practicum was operated based on Kolb's experiential learning cycle. For each class, students used mobile devices to write an online reflective journal. Analyzing contents of the reflective log helped in identifying difficulties experienced in executing core skills, and classifying them in terms of knowledge, skill, and attitude. The level of difficulty, importance, and confidence in the core clinical nursing skills were also assessed. Students were found to be struggling with various aspects of performing core nursing skills, especially in the skill category. Students also showed a lack of confidence in items they perceived as "high" difficulty, such as IV injection and indwelling catheterization. Moreover, over 50% students considered IV injection and vital sign checking as the most important core clinical nursing skills. Our data suggests the necessity to develop various contents and apply instructional strategies to solve the core skills difficulties faced by nursing students, and to continuously generate evidence for the same.
As new disasters such as COVID-19, MERS, and earthquakes appear in modern society, the nation's ability to manage uncertain risks is becoming more important. The government is promoting a disaster safety management policy closely related to daily life by reinforcing on-site response capabilities. Therefore, in order to respond more effectively to disasters that have recently been enlarged, complicated, and delocalized, there is a limit to only disaster-related organizations in the public sector. It is necessary to check it and find ways to develop it. In the event of an unpredictable national disaster or infection, the government needs disaster safety management measures closely related to daily life. Accordingly, as an efficient response and strategy such as procedures and methods for funeral support at the scene of a national disaster were needed, a designated funeral home was introduced. In the event of a major disaster, a large number of casualties that exceed the daily work level of the relevant department occur and rapid changes in relief, medical care, funeral and administrative procedures occur accordingly. The purpose of this study is to derive basic operating directions and prompt funeral support plans for funeral homes designated for national disaster preparedness.
Due to social and cultural changes and the growth of aging people living as a single because of aging, lonely deaths are steadily increasing, and each local government has begun to define them as a social problem. The legal basis began to be established. In order to explore policy measures to prevent lonely deaths, this study examined cases of lonely death prevention policies using smart digital information technology (AI, IOT), which is being promoted by Daegu Metropolitan City to promote non-face-to-face policies to prevent lonely deaths. Policies related to lonely deaths are divided into two axes: lonely death prevention projects and post-excavation support projects. In order to operate these businesses efficiently, the provision of non-face-to-face services through artificial intelligence and the Internet of Things is recognized as a new service delivery system, so the importance and necessity of non-face-to-face services is increasing. It is time that multifaceted changes and preparations are needed, such as establishing a system to expand the non-face-to-face industry at the national level. In order to respond to another national disaster situation in the future, the non-face-to-face smart care system is being expanded in various welfare policies such as preventing lonely deaths. It will have to be activated.
This study analyzed the impact on entrepreneurship, passion, and start-up execution willingness to start a business for 200 prospective start-ups in Jeollanam-do. As a result of this study, it was found that there was an effect at the statistically significant level (p<.05) in the relationship between the start-up support program and start-up willingness. In the mediating effect analysis of passion for entrepreneurship support programs, it was confirmed that passion played a complete mediating role in the relationship between mentoring and start-up willingness and a partial mediating role in the relationship between the start-up support fund and start-up willingness. In the analysis of the mediating effect of passion on entrepreneurship, it was confirmed that passion played a partial mediating role in the relationship between initiative and start-up execution willingness, and also in the relationship between risk sensitivity and start-up execution willingness. This shows that when proper initiative and risk tolerance are recognized, passion enhances the start-up willingness to run into the start-up market. Based on these results, we suggested theoretical implications and policy support directions to increase the willingness of prospective founders to implement.
This study was conducted to examine the relations of oral health relevance to disabilities according to the relationship types between them and their guardian. The guardians were surveyed using self-writing questionnaires regarding the cohesion and communication process, and the oral health of disabled was analyzed by evaluation table. Total 78 copies were analyzed. SPSS win. 24.0 statistical program was used and the significance level was verified at .05. There was significance in the communication process(p<.01) according to the type of relationship with the disabled and their guardians. In oral health of the disabled according to the type of relationship with their guardians, there was significance in DMFT index(p<.01), first molar soundness(p<.01) and Pocket depth(p<.01).Therefore, the mother had a positive effect for the disabled on most items such as cohesion, communication process, and oral health. However, it is expected that policy support and research to increase the interests in oral health care management are needed for personal assistants, who showed a low impact on the cohesion, and communication process with disabled persons, and spouses, who showed a low influence on oral health of persons with disabilities.
Journal of the Korea Academia-Industrial cooperation Society
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v.18
no.8
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pp.115-123
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2017
This study examined traditional dance programs in cultural and arts education in local children's centers. We aimed to contribute to the voluntary and active participation of children in cultural and arts education through differentiated cultural and arts educational programs. Interviews were conducted from March 2016- June, 2016 at A Regional Children's Center in Chungnam. Once a week, a 50-minute were held 16 Session as "learning traditional joyful dance". After completing the program, 25 children who participated in the program were asked about the good points and disappointments regarding the program. The interviews used open, structured questions that have been primarily used in phenomenological research. The list of questions was prepared in advance according to the research purpose. The results of the study are as follows: The evaluation of the first program was generally positive. By experiencing activities through cultural and arts education through traditional dance programs for children, children learned to be polite. It also changed positively in conversation. Children who showed negative attitudes toward traditional dance programs gradually participated in activities. Second, I examined the evaluation of lecturers who teach traditional dance. The children's response to the program was positive, with sincere and responsible lecture preparation and progress. The instructor prepared the class in advance, welcomed the children and ran the class in good faith. Instructors who taught traditional dances were proficient in dance, majored in dance and teaching children. The learning effect was high by the instruction of the good dance movement. Rich in lecture experience, he is proficient in dealing with children in class. Material was delivered according to the child's dance level.
Na, Hye-Won;Lee, Yeongran;Park, Jun Seong;Lee, Tae Ryoung;Kim, Hyoung-June
Journal of the Society of Cosmetic Scientists of Korea
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v.44
no.1
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pp.67-72
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2018
Green tea (Camellia sinensis L.) has been widely explored for its medicinal applications. However, most of the studies had targeted the green tea leaf, while other parts remained unexplored. In this study, protective effect of green tea root extract on Normal Human Epidermal Keratinocytes (NHEKs) against the damage induced by an external stimulant (PM2.5) was confirmed. Thirty-year-old green tea root samples were collected from Amorepacific's Dolsongi tea field and green tea root extract was prepared with 70% ethanol. Total crude saponin content in green tea root extract was 54%, which is much higher than that in ginseng extract. Our results suggest that green tea root extract can be used as a natural surfactant in cosmetics. For evaluating its protective effect against the damage induced by PM2.5, IL-36G was used as a biomarker. IL-36G mRNA expression level increased remarkable upon PM2.5 treatment in NHEKs. Moreover, IL-36G was recently reported to be expressed in psoriasis lesions. Results showed significant decrease of IL-36G expression by treatment of green tea root extract. In conclusion, thirty-year-old green tea root extract can be used as a natural surfactant with a high saponin content and may have protective effect against the damage induced by PM2.5.
The purpose of this study was to examine the effects of early intervention programs for child development and parenting stress from low-income families. The subjects of this study were 0-5 years old children 1,904(Participation 1,501 nonparticipating 403) and 1,904 parents from low-income families. The child and the parents who participated in the intervention program, 1,501 people respectively, did not participate in the intervention program for children and parents of 403 people each. The collected data were analyzed by t-test. The results of the study are as follows. First, There were differences in child development between the early child who participated in the intervention program and those who did not. Especially, the infants who participated in the intervention program had a more higher level in muscle exercise, small muscle exercises, problem solving, communication, and social development than infants who have not participated. Second, parents of low-income families who participated in the intervention program had lower parenting stress than the parents who didn't participate. As a result, early intervention programs should be targeted at children and parents from low-income families.
Choe, Jeong Sook;Ahn, Eun Mi;Kwon, Sung Ok;Park, Young Hee;Lee, Jinyoung
Journal of Nutrition and Health
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v.45
no.5
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pp.470-478
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2012
This study was conducted to investigate dietary factors, bone status, and bone loss in postmenopausal women in rural areas. A total of 189 women participated in the follow-up study after two years. Radius, Tibia, and Phalanx SOS (Speed of Sound) was measured on two occasions 2 years apart by ultra-sonic-metry, and % body fat was measured by bioelectrical impedance analysis at the baseline and after 2years. Dietary intake data were collected 4 times at different season by 24-hour recall method, and then calculated as average. Bone density of radius decreased by 4.2% during the two year period. When the subjects were divided into three groups, by bone decline level during two years, the lowest bone loss group had higher potassium and vegetable intake than other groups. Age and calcium intakes showed significant correlation with bone decline rate at tibia. In multiple regressions, the baseline SOS, vitamin A, vegetables and eggs intakes were found to be significant factors for tibia bone decline. In conclusion, dietary factors, such as higher vegetable intake, seem to affect the changes in bone mineral density in more favorable way. Therefore, efforts are needed to enhance the access to nutritional care for rural elderly postmenopausal women.
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