• 한국동물위생학회지
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• 제21권3호
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• pp.255-260
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• 1998

Since bovine leukosis caused considerable economic loss to the dairy industry, seroepidemiologi-cal survey on bovine leukosis was carried out for the dairy herds in Kyunggi province. 1. When compared the results of immunodifussion test with those of enzyme-linked immunosorbent assay(ELISA) for 94 dairy herds sera, the relationship between the immunodifussion test and ELISA were showen high corresponding rate with sensitivity(97.5%) and specificity(92.6%). 2. In immunodiffusion test for bovine leukosis virus (BLV) antibody in 570 dairy cattle from 30 herds, mean positive rate for BLV antibody was 28.2%. The positive rate by districts were 16.5% in central, 35.4% in east, 17.3% in west, 29.1% in south, 31.6% in north, 43.7% in northeast. 3. When the results of serological studies was analyzed by age groups, the number of positive was increased gradually with the advanced in age of herds. The highest positive rate was found in the age over 6 years. 4. Of 30 dairy herds examined, 5 herds(16.7%) have no reactions against BLV antigen while 15 herds (50%) showed the range of 1∼5 positive cattle and 5 herds(16.7%), the rang of over 11 positive cattle.

• 대한수의학회지
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• 제22권1호
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• pp.63-66
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• 1982

Authors encountered a case of bovine viral leukosis in a young cattle (10 months old) with the following findings; Main clinical symptoms observed were emaciation, digestive disorder, and marked enlargement of superficial lymph nodes. Biopsied specimen of the lymph nodes revealed characteristic histological picture of bovine leukosis. A severe degree of leukocytosis (108,000/cmm) accompanied by marked lympocytosis (96%=103,680/cmm) was detected from the hematological examination of peripheral blood. Most of the lympocytes(94%=101,520) were large-sized, immature, and abnormal in their morphology. In addition, a pronounced anemia (1.78 million/cmm) without regenerative evidence of erythrocyte was noted. The serum of the patient showed positive reaction against gp antigen of bovine leukosis virus in agar-gel diffusion test.

• 한국동물위생학회지
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• 제29권2호
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• pp.89-96
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• 2006

Bovine viral leukosis is a viral disease of cattle characterized by the development of tumors in the lymphatic tissue. A female Holstein, 3-year-old, was submitted for diagnosis at the Diagnostic laboratory, Chonbuk National University. Clinical sign of the affected animal showed emaciation, enlargement of superficial lymph node and mild diarrhea. Remarkable lesions were enlargement of many internal lymph nodes. Histopathology revealed excessive neoplastic lymphoid cells characteristic of BVL infection. Subsequently, serums from all cattle were collected and serological examination was done where a 85% seropositive rate was detected using ELISA test. ELISA method showed a comparatively 75% higher detection rate than the agar gel immunodiffusion (AGID) test (85% vs 40%). Serologically positive cattle were variably detected in all ages from under 1 year to over 6 year of age. Hematological examination consistently showed leukocytosis and a differential lymphocytosis of seropositive cattle. Detailed comparative pathological and serological data diagnosed the presence of bovine viral leukosis.

• 대한수의학회지
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• 제38권1호
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• pp.101-106
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• 1998

The present study attempted to apply polymerase chain reaction (PCR) to develop a rapid differential diagnosis among Marek's disease, reticuloendotheliosis and avian leukosis. The primers chosen to detect Marek's disease virus (MDV) flank the 132bp tandem direct repeat of the MDV genome. The primers selected for reticuloendotheliosis virus (REV) and avian leukosis virus (ALV) are based on proviral long terminal repeats of spleen necrosis virus and Rous-associated virus-2 genomes, respectively. The specific PCR products of MDV, REV and ALV were observed with each primer and the reaction was not cross-reacted among the viruses. MDV-specific DNA was also amplified from the MDV-induced lymphoma (MDCC-MSB1) but not from the REV-induced tumor and ALV-induced lymphoma (LSCC-1104B1). In addition, proviral DNA of REV from REV-induced tumor and proviral DNA of ALV from ALV-induced lymphoma were also amplified by REV-specific and ALV-specific PCRs, respectively. Therefore these three PCR methods may be used to rapidly differentiate among MDV, REV and ALV-associated tumors in diagnosis.

• 한국동물위생학회지
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• 제30권4호
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• pp.551-555
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• 2007

A slaughtered 3 years old cow was diagnosed as bovine leukosis by postmortem inspection, agar gel immunodifusion (AGID) test, and polymerase chain reaction (PCR). It showed no special clinical signs except emaciation. Grossly, we observed enlargement of mediastinal and pelvic lymph nodes, and tubercle -like mass on pleura and lung surface, and masses on right atrium and ventricle.

• 월간양계
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• 제15권통권165호
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• pp.112-117
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• 1983

• 월간양계
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• 제4권6호통권32호
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• pp.73-73
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• 1972

• 대한수의학회지
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• 제30권3호
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• pp.333-341
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• 1990

To investigate bovine leukosis virus (BLV) infection in the cattle rearing in a dairy farm where a case of bovine lymphosarcoma had been identified several years ago, the 196 Holstein cattle including newborn calves to 12 years of age were tested. The BLV antibody test and peripheral lymphocyte count for bovine leukosis were carried out by the immunodiffusion (ID) test and Bendixen's Kep. These tests were performed 2 to 4 times at the interval of 3 to 5 months. The observed results were as follows: 1. The positive rates of BLV-antibody in the 1st, the 2nd, the 3rd and the 4th tests were 23.3%, 28.1%, 49.0% and 55.7%, respectively. The conversion rates from negative to positive in the 2nd, the 3rd and the 4th tests were 8.9%, 41.4%, and 20.0%, respectively. Results showed that the highest conversion rate was observed at the 3rd test which was conducted after winter. The highest positive rate by ID test were observed in 4 year old cattle in the 1st and 2nd tests, and in 2 year old herd in the 3rd and 4th tests. 2. In hematological test by Bendixen's Key, the positive and suspicious rates in the 1st, the 2nd, the 3rd and the 4th tests were 5.8 and 7.8%, 8.3 and 6.6%, 8.7 and 10.1%, 10.8 and 19.6% respectively. Results showed that the positive and. suspicious rates increased in course of time. 3. 70 to 100% of the positive cattle in hematological test were positive for BLV-antibody test. All of 13 cattle with persistent lymphocytosis (PL) were also positive for BLV-antibody, indicating the high relationship between PL and BLV-antibody. 4. The number of total leukocytes and absolute lymphocytes in the BLV-antibody positive cattle appeared significantly higher than those of the negative cattle. The markedly increased cell counts were observed in the cattle over one year old. 5. The mean of total leukocytes and absolute lymphocytes in the negative cattle for BLV-antibody increased slightly after sero-conversion into positive. 6. In the clinical examinations during experimental periods, none of the 72 positive cattle for BLV-antibody showed any lesions for bovine leukosis.

• 대한수의사회지
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• 제14권4호
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• pp.239-252
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• 1978

Two diotinot lymphomatous diseases occur in the field in domestic fowl: Lymphoid leukosis, which is caused by an oncornavirus and Marek's disease, which is caused by a herpesvirus. They are the most common neoplastic diseases of the chicken, and Marek's d

• 대한수의학회지
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• 제14권1호
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• pp.49-51
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• 1974

Nephroblastoma of avian leukosis/sarcoma group was encountered in a Shaver pullet. The tumor, measuring $10{\times}5mm$ in base and 5 mm in height, was located on the surface of anterior lobe of the left kidney. The tumor mass was irregular in shape, firm in consistency and greyish white in color. Histologically, the neoplastic tissues consisted of embryonal uriniferous tubules and fibrosarcomatous stroma.