• Journal of Nutrition and Health
• /
• 제51권4호
• /
• pp.316-322
• /
• 2018

Purpose: The Glycyrrhiza uralensis species (Leguminosae) as a medicinal biocompound, and one of its root components, isoliquritigenin (ISL), which is a flavonoid, has been reported to have anti-tumor activity in vitro and in vivo. However, its function in bone formation has not been studied yet. In this study, we tested the effect of Glycyrrhiza uralensis (ErLR) and baked Glycyrrhiza uralensis (EdLR) extracts on osteoblast proliferation, alkaline phosphatase (ALP) activity, and bone-related gene expression in osteoblastic MC3T3-E1 cells. Methods: MC3T3-E1 cells were cultured in various levels of ErLR (0, 5, 10, 15, $20{\mu}g/mL$), EdLR (0, 5, 10, 15, $20{\mu}g/mL$), or ISL (0, 5, 10, 15, $20{\mu}M$) in time sequences (1, 5, and 20 days). Also, isoliquritigenin (ISL) was tested for comparison to those two biocompound extracts. Results: MTT assay results showed that all three compounds (ErLR, EdLR, and ISL) increased osteoblastic-cell proliferation in a concentration-dependent manner for one day. In addition, both ErLR and EdLR compounds elevated the osteoblast proliferation for 5 or 20 days. Extracellular ALP activity was also increased as ErLR, EdLR, and ISL concentration increased at 20 days, which implies the positive effect of Glycyrrhiza species on osteoblast mineralization. The bone-related marker mRNAs were upregulated in the ErLR-treated osteoblastic MC3T3-E1 cells for 20 days. Bone-specific transcription factor Runx2 gene expression was also elevated in the ErLR- and EdLR-treated osteoblastic MC3T3-E1 cells for 20 days. Conclusion: These results demonstrated that Glycyrrhiza uralensis extracts may be useful for preventing osteoporosis by increasing cell proliferation, ALP activity, and bone-marker gene expression in osteoblastic cells.

• Natural Product Sciences
• /
• 제14권3호
• /
• pp.187-191
• /
• 2008

The contents of isoflavones and 1,1-diphenyl-2-picryl hydrazyl (DPPH) free radical scavenging activities were determined in flowers (Puerariae Flos) and roots (Puerariae Radix) of Pueraria thunbergiana to differentiate both pharmacochemical or pharmacognostical usage of both oriental medicinal drugs. The tectorigenin contents in flower before and after acid hydrolysis were shown to be 17.10 ${\mu}mole\;g^{-1}$ and 49.58 ${\mu}mole\;g^{-1}$, respectively, analyzed by HPLC. However, the root displayed much less content of tectorigenin even after acid hydrolysis (6.56 ${\mu}mole\;g^{-1}$) than in the flower. Acid hydrolysis made glycitin and tectoridin of the isoflavone glycosides almost disappear in the flower and root. DPPH assay results demonstrated that acid hydrolysis of both extracts or both glycosides should increase free radical-scavenging activities due to the increase of isoflavone contents. It is also suggested that the flower had much higher concentration of isoflavonoids than in the root.

• Journal of Applied Biological Chemistry
• /
• 제51권6호
• /
• pp.253-261
• /
• 2008

Isoflavone daidzein is a phytoestrogen widely distributed in Leguminosae and is especially rich in the soybean. The C6-C3 (rings B and C) unit of isoflavones is derived from the phenylpropanoid pathway and the remaining C6 (ring A) unit is from the polyketide pathway. This unique carbon skeleton is the result of isomerization of the flavone catalyzed by the isoflavone synthase, a cytochrome P450 enzyme. The isoflavones daidzein and genistein are present in the plant mostly in the glucosylated forms. However, in the human intestine, the glycosidic linkage is broken, and the free form is uptaked into blood stream. The free form is further metabolized into various reduction products to end up at the equol, which is known to have the most potent estrogenic effect among the metabolites. Several human intestinal bacteria that can convert daidzein into equol have been described, and the study into the chemistry and biochemistry of the daizein reduction would be rewarding to the improvement of the human health.

• 대한화장품학회:학술대회논문집
• /
• 대한화장품학회 2003년도 IFSCC Conference Proceeding Book I
• /
• pp.291-312
• /
• 2003

Reactive oxygen species are capable of damaging biomolecules such as lipids, proteins, and DNA, which can not only lead to various diseases, but also oxidative damage resulting aging. In our previous study, Cercis chinensis (Leguminosae) showed a potent antioxidant activity. Nineteen compounds were isolated through antioxidant activity-guided fractionation. The C. chinensis extract and some of the constituents exhibited a potent antioxidant activity on the free radicals and lipid peroxidation and a notable protective effect on the t-BuOOH induced oxidative damage. In vivo test of skin damage induced by UVB irradiation, the extract of C. chinensis and a constituent, piceatannol, exhibited a significant protective effect. The life-span of the HEK-N/F cells were extended by 1.21-2.12 fold as a result of the continuous administration of 3 $\mu\textrm{g}$/ml of the C. chinensis extract and the active constituents compared to that of the control. These observations were attributed to the inhibitory effect of the C. chinensis extract and its constituents on the age-dependent shortening of the telomere. Thus, C. chinensis was demonstrated to protect the skin cells against oxidative stress and inhibit thereby the cellular aging, followed by expectation as anti-aging cosmetic ingredient.

• 한국자원식물학회지
• /
• 제1권1호
• /
• pp.53-71
• /
• 1988

전남지역의 명산중의 하나인 백운산 지역에 분포되어 있는 한약자원 식물을 수집 및 분류하여 약용작물로 재배하기 위한 기초자료로 활용하고자 이 지역의 한약자원 식물의 분포를 조사한 결과는 다음과 같았다. 1. 백운산에 분포되어 잇는 약용자원은 60과 250종으로 분류되었다. 2. 분포수종중 과본류는 161 종 목본류 89종이 분포되어 있었다. 3.과본류는 사삼, 도라지, 쑥, 엉겅퀴, 질경이, 차조기, 쇠무릎, 반하, 하수오, 결명자, 용담초, 맥문동, 하고초, 둥글레, 참마, 칡 등이 많이 분포되어 있었고, 목본류로는 굴피나무, 가래나무, 호두나무, 후피향나무, 모란, 이팝나무, 쥐똥나무, 누리장나무, 고로쇠나무, 비자나무, 삼나모, 때죽나무, 모과나무 등이 한약재료로 많이 사용되는 것들이었다.

• 한국자원식물학회지
• /
• 제1권1호
• /
• pp.23-32
• /
• 1988

전남지역에서 3대 명산중의 하나인 모후산 지역에 분포되어 있는 한약자원식물을 수집 및 분류하여 약용작물로 재배하기 위한 기초자료로 활용하고자 이 지역의 한약자원 식물의 분류를 조사한 결과는 다음과 같았다. $\bullet$ 모후산에 분포되어 있는 한약자원 식물은 58과 230종으로 분류되었다. $\bullet$ 분류수종중 초본류는 질경이, 차조기, 쇠무릎, 우엉, 사삼, 반하, 하수오, 결명자, 용담초, 맥문동, 하고초, 산삼, 도라지, 둥글레, 참마, 칡, 대계등 17종과 대본류는 인동초, 참으아리, 방기, 목퉁, 엄나무, 구기자, 산수유, 밤나무, 매화, 인진호, 희첨, 지유, 초오등 13종이 한약재료로 많이 사용되는 것들이었다.

• 한국식품위생안전성학회지
• /
• 제3권1호
• /
• pp.1-5
• /
• 1988

현재 한국에서 시판되고 있는 화분식품 16종을 검사하여 본 바, 15 종에서 원형 그대로의 화분임을 확인하였다. 따라서 이들 화분의 기원식물을 감별하였는 바, 국화과, 백합과, 사초과, 층층나무과, 소나무과, 자작나무과, 쥐손이풀과, 콩과, 참나무과, 벼과의 식물 화분이었다. 이들 화분은 그 표피를 그대로 지니고 있으므로 식이성 allergen으로 작용할 우려가 있다. 16종의 화분식품중 한가지 만이 화분 표피를 함유하고 있지 않았다.

• Advances in Traditional Medicine
• /
• 제10권2호
• /
• pp.66-78
• /
• 2010

Acute (24 h) and chronic (90 days) oral toxicity studies on the ethanol extract of Trigonella foenumgraecum Leguminosae (L.) seeds were carried out. Acute dosages were 0.5, 1.0 and 3 g/kg while chronic dosage was 100 mg/kg per day of the extract. All morphological, biochemical, haematological and spermatogenic changes, in addition to mortality, body weight changes and any change in vital organs were recorded. Histopathological investigations were done on vital organs. Growth arrest in the treated animals was observed. The treated mice gained no significant weight during chronic treatment while there was a significant gain in body weight of the control group mice. Biochemical studies revealed a significant decrease in blood sugar levels of fenugreek treatment groups while haematological parameters remained comparable to the control. In the treatment, male group there was a significant decrease in weight of testes as compared to the control. There was a marginal weight gain in kidney weight of mice after chronic treatment as compared to the control. Fenugreek chronic treatment caused a highly significant spermatotoxic effects in male mice.

• Natural Product Sciences
• /
• 제5권3호
• /
• pp.127-133
• /
• 1999

'Gunja' is attributable to the plant Abrus precatorius L. (Leguminosae). Three forms with red, brown and another with white seeds are known to occur in this species and are employed for different therapeutic uses viz. as purgative, emetic, aphrodisiac, tonic and also as an abortifacient. According to some Ayurvedic literature the seeds are poisonous and should be given to the patients after proper processing ('Shodhan'). A comparative study of various phytochemical parameters, namely, percentage of successive extractives, total proteins, tannins, total ash and acid insoluble ash of these three forms of the processed (with cow's milk and Kanji) and unprocessed seeds was done. TLC and densitometric scanning of successive extractives was also carried out to serve as markers for processed and unprocessed seeds. The percentage of proteins, tannins, alcohol and water soluble extractives decreased in the processed material. Besides, their acute toxicity, CNS activity were also studied in albino mice and it was found that white seeds are more toxic as compared to the red and brown. The toxic effect was reduced with the processing. Further, the 'Kanji' processed seeds are less toxic than the milk processed one.

• 생약학회지
• /
• 제27권3호
• /
• pp.196-206
• /
• 1996

In order to characterize the chemical change, of the constituents and pharmacological transformation of Glycyrrhizae Radix(GR) which may occur during its processing, processed GR has been investigated in comparison with unprocessed GR. It was found that glycyrrhizin(GL) and other several constituents contained in GR were remarkably changed by processing, and the decrease of contents of GL was identified by making use of thin layer chromatography-chromatoscanner. And also, it was found that glycyrrhetic acid (GA) was identified from processed GR. We recognized that commonly processed GR showed less hemolytic effect than unprocessed GR and processed GR at $190{\circ}c$. It was also found that commonly processed GR showed more effective analgesic effect and prolonged the duration of hypnosis induced by pentobarbital-Na in mice as compared with corresponding unprocessed GR and processed GR at $190{\circ}c$. And then, commonly processed GR significantly suppressed the increases of s-GOT, s-GPT and s-LDH activities in injured mice induced by $CCl_4$.