• Journal of Food Hygiene and Safety
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• v.38 no.2
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• pp.69-78
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• 2023

This study aimed to investigate the protective effect of enzymatically modified stevia (EMS) on C2C12 cell-based model of dexamethasone (DEX)-induced muscle atrophy to provide baseline data for utilizing EMS in functional health products. C2C12 cells with DEX-induced muscle atrophy were treated with EMS (10, 50, and 100 ㎍/mL) for 24 h. C2C12 cells were treated with EMS and DEX to test their effects on cell viability and myotube formation (myotube diameter and fusion index), and analyze the expression of muscle strengthening or degrading protein markers. Schisandra chinensis Extract, a common functional ingredient, was used as a positive control. EMS did not show any cytotoxic effect at all treatment concentrations. Moreover, it exerted protective effects on C2C12 cell-based model of DEX-induced muscle atrophy at all concentrations. In addition, the positive effect of EMS on myotube formation was confirmed based on the measurement and comparison of the fusion index and myotube diameter when compared with myotubes treated with DEX alone. EMS treatment reduced the expression of muscle cell degradation-related proteins Fbx32 and MuRF1, and increased the expression of muscle strengthening and synthesis related proteins SIRT1 and pAkt/Akt. Thus, EMS is a potential ingredient for developing functional health foods and should be further evaluated in preclinical models.

• Journal of Applied Biological Chemistry
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• v.57 no.3
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• pp.189-196
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• 2014

In Korea, over 4,700 animal carcass disposal sites were installed until 2011 due to the outbreak of foot and mouth disease. Due to the putrefaction of buried animals, the leachate containing veterinary antibiotics may release into surrounding environments. Antibiotic residues in the environment cause the formation of antibiotic resistance bacteria threatening human and ecosystem health. This study reports the concentrations of five antibiotics, including tetracycline (TC), chlortetracycline (CTC), oxytetracycline (OTC), sulfamethazine (SMZ), and sulfamethoxazole (SMX), in soils from animal carcass disposal site and adjacent agricultural field. Concentrations of antibiotics at animal carcass disposal sites (TC: $144.26-350.73{\mu}g/kg$, SMZ: $17.72-44.94{\mu}g/kg$) were higher than those at agricultural field (TC: $134.16-320.73{\mu}g/kg$, SMZ: $6.48-8.85{\mu}g/kg$) whereas the concentrations of CTC, OTC, and SMX were below detection limit in both sites. Results showed that the antibiotics in animal carcass site might leach to the soil and possibly contaminating the groundwater. Future studies will focus on the transfer of antibiotics residues into food crops.

• Journal of the Korean Society for Railway
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• v.15 no.4
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• pp.413-421
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• 2012

The purpose of this study is to evaluate the structural dynamic behavior under hybrid control system. The hybrid test is to consider the interaction between the numerical and physical models. In this paper, single degree of freedom hybrid test was performed with one-bay, two-story steel frame structure. One column at the first floor was selected as a physical substructure and one actuator was used for applying the displacement load in horizontal direction. El Centro as earthquake waves was inputted and OpenSees was employed as the numerical analysis program for the hybrid real-time simulation. As a result, the total time of the hybrid test was about 9.6% of actual measured seismic period. The experimental results agreed well with the numerical one in terms of the maximum displacement. In nonlinear analysis, however, material nonlinearity made a difference of residual strain. Therefore, this hybrid dynamic test can be used to predict the structural dynamic performance more effectively than shaking table test, because of the spatial and economic limitations.

• Journal of Korean Ophthalmic Optics Society
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• v.16 no.4
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• pp.363-373
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• 2011

Purpose: To establish basic data for effective management and quality improvement on clinical practice by investigating the clinical practice satisfaction of ophthalmic optics students and optical shop owners. Methods: A survey was administered for 281 students with experience in clinical practices and 105 optical shop owners regarding satisfaction of ophthalmic optics students and optical shops. And a statistical analysis was performed on the survey results. Results: 92.% (97 shops) of optical shop owners responded that clinical practice was necessary and there was a significant difference (p=0.004) according to gender. 4 weeks was the preferred length of practice period (53.3%) and there was a significant difference according to the size of the optical shops (p=0.000). Clinical practice satisfaction of students was 3.50 ${\pm}$ 0.68 and satisfaction for clinical practice program, clinical practice time and clinical practice shop (3.74 ${\pm}$ 0.80) were the highest and there was a significant difference according to the age of the students (p<0.05). Clinical practice satisfaction for optical shops was 4.08 ${\pm}$ 0.64 which is higher than student satisfaction. Satisfaction for clinical practice program was (4.17 ${\pm}$ 0.54) was the highest. Conclusions: To maximize the effects of clinical practice, a clinical practice program reflecting the satisfaction of students and optical shops is required and further research and attention are required.

• Journal of Life Science
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• v.15 no.5 s.72
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• pp.783-789
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• 2005

Vibriocidal GOCHUJANG was manufactured using extract of natural products and green tea. Antioxidative capacity of natural products and combination of natural products was measured with Oxidation-Reduction Potential (ORP) system value. Inhibitory ability of vibriocidal GOCHUJANG was compared with Inhibition Zone Diameter (IZD) values. Phellodendri cortex, Schizandrae fructus, Theae folium, Scutellaria baicalensis george and Acanthodanacis cortex and combination of these natural products were showed higher antioxidant capacity and inhibitory effect against V. para-haemolyticus. Two kinds of combinations, A combination was composed of Phellodendri cortex, Schizandrae fructus, Theae folium, Scutellaria baicalensis george, and B combination was consisted of Acanthodanacis cortex and A combination, were presented higher inhibition ratio with IZD values as $1.57\pm0.0051$ and $1.56\pm0.0071$, respectively. Vibriocidal GOCHUJANG was prepared based on A and B combination with their higher IZD Value as $1.76\pm0.0103$ and $1.79\pm0.0103$, respectively. When vinegar was added to A and B based GOCHUJANG, their IZD value was more higher as $2.15\pm0.0071$ and $2.44\pm0.0086$, respectively B combination based GOCHUJANG was exhibited higher inhibitory effect than A based GOCHUJANG. Combination of natural products was more important process for increasing vibriocidal effect. But addition of soybean malt or chitosan or a garlic into A or B combined GOCHUJANG, no different effect was exhibited.

• ALGAE
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• v.35 no.3
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• pp.263-275
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• 2020

Water temperature is known to affect the growth and feeding of marine dinoflagellates. Each dinoflagellate species grows well at a certain optimal temperature but dies at very cold and hot temperatures. Thus, changes in water temperatures driven by global warming and extremely high or low temperatures can affect the distribution of dinoflagellates. Yihiella yeosuensis is a mixotrophic dinoflagellate that can feed on only the cryptophyte Teleaulax amphioxeia and the chlorophyte Pyramimonas sp. Furthermore, it grows fast mixotrophically but rarely grows photosynthetically. We explored the direct and indirect effects of water temperature on the growth and ingestion rates of Y. yeosuensis feeding on T. amphioxeia and the growth rates of T. amphioxeia and Pyramimonas sp. under 7 different water temperatures (5-35℃). Both the autotrophic and mixotrophic growth rates of Y. yeosuensis on T. amphioxeia were significantly affected by temperature. Under the mixotrophic and autotrophic conditions, Y. yeosuensis survived at 10-25℃, but died at 5℃ and ≥30℃. The maximum mixotrophic growth rate of Y. yeosuensis on T. amphioxeia (1.16 d^-1) was achieved at 25℃, whereas the maximum autotrophic growth rate (0.16 d^-1) was achieved at 15℃. The maximum ingestion rate of Y. yeosuensis on T. amphioxeia (0.24 ng C predator^-1 d^-1) was achieved at 25℃. The cells of T. amphioxeia survived at 10-25℃, but died at 5 and ≥30℃. The cells of Pyramimonas sp. survived at 5-25℃, but died at 30℃. The maximum growth rate of T. amphioxeia (0.72 d^-1) and Pyramimonas sp. (0.75 d^-1) was achieved at 25℃. The abundance of Y. yeosuensis is expected to be high at 25℃, at which its two prey species have their highest growth rates, whereas Y. yeosuensis is expected to be rare or absent at 5℃ or ≥30℃ at which its two prey species do not survive or grow. Therefore, temperature can directly or indirectly affect the population dynamics and distribution of Y. yeosuensis.

• Korean Journal of Soil Science and Fertilizer
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• v.47 no.6
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• pp.525-532
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• 2014

Since mine wastes were merely dumped in the mine waste dump, they have produced acid mine drainage (AMD). Therefore, main objective of this study was to evaluate the effect of coal combustion products (CCPs) on heavy metal stabilization and detoxification for mine wastes. Total six treatments for incubation test were conducted depending on mixing method (completely mixing and layered). Also, lysimeter experiment was conducted to examine efficiency of polyacrylamide (PAM) on reduction of mine wastes erosion. Result of incubation test showed that concentrations of soluble aluminium (Al) and iron (Fe) in leachate decreased compared to control. The lowest soluble Al and Fe in leachate was observed in 50% mixed treatment (14.2 and $1.03mg\;kg^{-1}$ for Al and Fe respectively) compared to control treatment (253.0 for Al and $52.6mg\;kg^{-1}$ for Fe). The pH of mine wastes (MW) and leachate increased compared to control after mixing with CCPs and ordered as control (MW 6.4, leachate 6.3) < 10% (MW 7.7, leachate 7.1) < 20% (MW 9.0, leachate 7.8) < 30% (MW 9.5, leachate 8.3) < 40% (MW 9.9, leachate 8.5) < 50% (MW 10.5, leachate 8.6). Application of PAM, both in liquid and granular type, dramatically decreased the suspended solid (SS) concentration of CCPs treatments. Reduction of SS loss was ordered as MW70CR30L ($24.4mg\;L^{-1}$) > MW70CR30LPL ($6.7mg\;L^{-1}$) > NT ($3.1mg\;L^{-1}$) > MW70CR30M ($1.6mg\;L^{-1}$) > MW70CR30MPL ($1.1mg\;L^{-1}$) > MW70CR30PGM ($0.7mg\;L^{-1}$) > MW70CR30LPG ($0.5mg\;L^{-1}$) > MW70CR30MPG ($0.4mg\;L^{-1}$). Overall, application of CCPs can be environmental friendly and cost-effective way to remediate coal mine wastes contaminated with heavy metals. In addition, use of PAM could help to prevent the erosion coal mine wastes in mine waste disposal area.

• Proceedings of the Korea Water Resources Association Conference
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• 2005.05b
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• pp.879-883
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• 2005

우리나라는 하천유역의 도시화 추세 속에 불투수층의 증가로 빗물의 일시 유출로 인한 홍수발생으로 많은 인명과 재산피해가 발생하고 있어 방재적 차원에서의 수자원관리가 시급한 실정이다. 또한, 초기 빗물과 합류식 하수도의 월류수에 의한 하천, 호소, 및 습지의 수질오염문제도 많이 발생하고 있다. 이러한 문제를 해결하기 위하여 콘크리트로 제작된 PC 지하식 빗물저류시설로서 상부의 공간은 공원, 운동장, 주차장 등 다양하게 이용하면서 방재와 치수를 가능케 할 수 있다. 또한, PC 콘크리트 빗물저류조는 현장 타설이 아닌 PC콘크리트 블록을 현장에서 조립하여 시공기간이 대폭적으로 단축되고, 작업환경 및 주변환경을 개선시킬 수 있다. 또한, 지하수의 보전, 회복을 위한 빗물저류 침투 시설 역할도 수행하여 비상용수를 확보하고 여름철 홍수 시 빗물을 가두어 재해를 방지하는 등의 다목적 시설로 활용된다. 지하 매립형 빗물저류조는 기존의 암거설계기준을 참조하여 일본의 내진설계 기준을 반영하였으며, 고강도 콘크리트를 사용하여 강도 또한 뛰어나다. 그리고 시공이 간편하고 공기의 단축에 탁월한 효과를 나타내며, 빗물저류조 설치는 다음과 같은 특징이 있다. 1. 지하저류형 빗물저류조 시설로 설계되어 토지의 효과적인 이용이 기대된다. 2. 공사기간이 짧아 경제적이다. 3. 안정된 구조체이다. 4. 부지의 형태에 맞춘 시공이 가능하다. 5. 소규모에서 대규모의 유수지까지 광범위하게 대응이 가능하다. 6. 방재역할 수행 및 빗물이용의 역할을 담당할 수 있다. 7. 불투수층이 증가하고 있는 도시지역에서 적극 활용가능하다.로 판단된다.한 예비방류의 시행과 강우종료 후에도 이수용량에는 손실이 없는 저수지의 관리방안의 지침이 되는데 효율적이라 판단되었다. 방법을 개발하여 개선시킬 필요성이 있다.>$4.3\%$로 가장 근접한 결과를 나타내었으며, 총 유출량에서도 각각 $7.8\%,\;13.2\%$의 오차율을 가지는 것으로 분석되어 타 모형에 비해 실유량과의 차가 가장 적은 것으로 모의되었다. 향후 도시유출을 모의하는 데 가장 근사한 유출량을 산정할 수 있는 근거가 될 것이며, 도시재해 저감대책을 수립하는데 기여할 수 있을 것이라 판단된다.로 판단되는 대안들을 제시하는 예비타당성(Prefeasibility) 계획을 수립하였다. 이렇게 제시된 계획은 향후 과학적인 분석(세부평가방법)을 통해 대안을 평가하고 구체적인 타당성(feasibility) 계획을 수립하는데 토대가 될 것이다.{0.11R(mm)}(r^2=0.69)$로 나타났다. 이는 토양의 투수특성에 따라 강우량 증가에 비례하여 점증하는 침투수와 구분되는 현상이었다. 경사와 토양이 같은 조건에서 나지의 경우 역시 $Ro_{B10}(mm)=20.3e^{0.08R(mm)(r^2=0.84)$로 지수적으로 증가하는 경향을 나타내었다. 유거수량은 토성별로 양토를 1.0으로 기준할 때 사양토가 0.86으로 가장 작았고, 식양토 1.09, 식토 1.15로 평가되어 침투수에 비해 토성별 차이가 크게 나타났다. 이는 토성이 세립질일 수록 유거수의 저항이 작기 때문으로 생각된다. 경사에 따라서는 경사도가 증가할수록 증가하였으며

• Reproductive and Developmental Biology
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• v.34 no.3
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• pp.127-134
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• 2010

This study was to investigate the effect of flavonoid treatment on in vitro development of bovine somatic cell nuclear transfer (SCNT) embryos, and their pregnancy and delivery rate after embryo transfer into recipient. In experiment 1, to optimize the flavonoid concentration, parthenogenetic day 2 ($\geq$ 2-cell) embryos were cultured in 0 (control), 1, 10 and $20\;{\mu}M$ flavonoid for 6 days. In the results, in vitro development rate was the highest in $10\;{\mu}M$ flavonoid group (57.1%) among treatment groups (control, 49.5%; $1\;{\mu}M$, 54.2%; $20\;{\mu}M$, 37.5%), and numbers of total and ICM cells were significantly (p<0.05) higher in $10\;{\mu}M$ flavonoid group than other groups. We found that $10\;{\mu}M$ flavonoid treatment can significantly (p<0.05) decrease the apoptotic index and derive high expression of anti-oxidant, anti-apoptotic, cell growth and development marker genes such as Mn-SOD, Survivin, Bax inhibitor, Glut-5, In-tau, compared to control group. In experiment 2, to produce the cloned Jeju Black Cattle, beef quality index grade 1 bull somatic cells were transferred into enucleated bovine MII oocytes and reconstructed embryos were cultured in $10\;{\mu}M$ flavonoid added medium. When the in vitro produced day 7 or 8 SCNT blastocysts were transferred into a number of recipients, $10\;{\mu}M$ flavonoid treatment group presented higher pregnancy rate (10.2%, 6/59) than control group (5.9%, 2/34). Total three cloned Jeju Black calves were born. Also, two cloned calves in $10\;{\mu}M$ flavonoid group were born and both were all healthy at present, while the one cloned calf born in control group was dead one month after birth. In addition, when the result of short tandem repeat marker analysis of each cloned calf was investigated, microsatellite loci of 11 numbers matched genotype between donor cell and cloned calf tissue. These results demonstrated that the flavonoid addition in culture medium may have beneficial effects on in vitro and in vivo developmental capacity of SCNT embryos and pregnancy rate.

• The Korean Journal of Community Living Science
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• v.28 no.1
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• pp.59-68
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• 2017

In this study, two kinds of chestnut-sweet potato (CSP) and pumpkin-sweet potato (PSP) were cooked by three methods: raw, steaming, and roasting. These samples were investigated in food compositions such as moisture, protein, ash, dietary fiber, fat, minerals, and vitamins. As the results of this study, the moisture contents of raw CSP and raw PSP were higher than those of steamed and roasted samples in two cultivars. The contents of protein in raw CSP and raw PSP were 2.57 g/100 g and 3.22 g/100 g, respectively, which were higher than those of other cultivars. The protein contents of roasted CSP and steamed PSP were lower than those of their raw samples. The potassium, phosphorus, magnesium, calcium, sodium, and iron values of PSP were 1,048.46 mg/100g, 152.02 mg/100g, 74.70 mg/100g, 57.22 mg/100g, 22.28 mg/100g, and 1.44 mg/100g, respectively, which were the highest values in tested sweet potato cultivars. The content of dietary fiber in CSP was higher generally than that in PSP. The values of total dietary fiber in cooked sweet potatoes were higher than those of raw sweet potatoes. The contents of vitamins, including thiamine, riboflavin, and niacin, in PSP were higher than those in CSP. Overall, two cultivars of raw, boiled, and roasted sweet potatoes had enhanced food composition. Therefore, these sweet potato cultivars are expected to be highly valuable food items for the development and application of functional foods.