• Title/Summary/Keyword: Lee Sam

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Evaluation of Decomposition Characteristics of Organochlorine Pesticides Using Thermal Method (열적방법을 활용한 유기염소계 폐농약의 분해 특성 평가)

  • Kwon, Eun-Hye;Yoon, Young-Sam;Bea, Ji-Su;Jeon, Tae-Wan;Lee, Young-Ki
    • Journal of Korea Society of Waste Management
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    • v.35 no.8
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    • pp.744-753
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    • 2018
  • The Stockholm Convention, which was adopted in Sweden in 2001 to protect human health and the environment, includes regulations for Persistent Organic Pollutant Rotors such as toxic and bioaccumulatives. Currently, there are 28 types of materials. This prohibits and limits the production, use, and manufacture of products. Korea is a member of the Convention, and it is necessary to prepare management and treatment plans to address the POP trends. Thus, we experimentally investigate whether the environmentally stable incineration is achieved when the sample is thermally treated using the Lab-scale (1 kg/hr). The target samples is pesticides in liquid phase and solid phase. In this study, organic chlorinated pesticides and their thermal characteristics were analyzed. We calculated the theoretical air volume based on the element analysis results. Because the interior of the reactor is small, more than 10 times of the air ratio was injected. The retention time was set to at least 4 seconds using a margin. The incineration temperature was $850^{\circ}C$ and $1100^{\circ}C$. Thus, we experimentally investigated whether the environmentally stable incineration was achieved when the sample was thermally treated using the Lab-scale (1 kg/hr). We analyzed five types of exhaust gas; the 02 concentration was high, but the CO amount decreased. Complete combustion is difficult because of the small size of the furnace due to the nature of Lab-scale. The organic chlorine-containing pesticide had an average decomposition rate of 99.9935%. Considering the decomposition rates of organic chlorine-containing pesticide in this study, the incineration treatment at over 2 ton/hour, which is typical for a conventional incinerator, is possible. Considering the occurrence of dioxins and unintentional persistent organic pollutants, it can operate at more than $1,100^{\circ}C$.

Design and Implementation K-Band EWRG Transceiver for High-Resolution Rainfall Observation (고해상도 강수 관측을 위한 K-대역 전파강수계 송수신기 설계 및 구현)

  • Choi, Jeong-Ho;Lim, Sang-Hun;Park, Hyeong-Sam;Lee, Bae-Kyu
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.24 no.5
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    • pp.646-654
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    • 2020
  • This paper is to develop an electromagnetic wave-based sensor that can measure the spatial distribution of precipitation, and to a electromagnetic wave rain gauge (hereinafter, "EWRG") capable of simultaneously measuring rainfall, snowfall, and wind field, which are the core of heavy rain observation. Through this study, the LFM transmission and reception signals were theoretically analyzed. In addition, In order to develop a radar transceiver, LFM transceiver design and simulation were conducted. In this paper, we developed a K-BAND pulse-driven 6W SSPA(Solid State Power Amplifiers) transceiver using a small HMIC(Hybrid Microwave Integrated Circuit). It has more than 6W of output power and less than 5dB of receiving NF(Noise Figure) with short duty of 1% in high temperature environment of 65 degrees. The manufactured module emits LFM and Square Pulse waveform with the built-in waveform generator, and the receiver has more than 40dB of gain. The transceiver developed in this paper can be applied to the other small weather radar.

Numerical Simulation of Wave Pressure Acting on Caisson and Wave Characteristics near Tip of Composite Breakwater (for One Directional Irregular Waves) (혼성방파제 케이슨에 작용하는 파압과 선단 주변에서 파랑특성에 관한 수치모의(일방향불규칙파에 대해))

  • Jun, Jae-Hyoung;Choi, Goon-Ho;Lee, Kwang-Ho;Kim, Do-Sam
    • Journal of Korean Society of Coastal and Ocean Engineers
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    • v.32 no.6
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    • pp.531-552
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    • 2020
  • In the previous study, both the wave characteristics at the tip of composite breakwater and on caisson were investigated by applying olaFlow numerical model of three-dimensional regular waves. In this paper, the same numerical model and layout/shape of composite breakwater as applied the previous study under the action of one directional irregular waves were used to analyze two and three-dimensional spatial change of wave force including the impulsive breaking wave pressure applied to trunk of breakwater, the effect of rear region, and the occurrence of diffracted waves at the tip of caisson located on the high crested rubble mound. In addition, the frequency spectrum, mean significant wave height, mean horizontal velocity, and mean turbulent kinetic energy through the numerical analysis were studied. In conclusion, the larger wave pressure occurs at the front wall of caisson around the still water level than the original design conditions when it generates the shock-crushing wave pressure in three-dimensional analysis condition. Which was not occurred by two-dimensional analysis. Furthermore, it was confirmed that the wave pressure distribution at the caisson changes along the length of breakwater when the same significant incident wave was applied to the caisson. Although there is difference in magnitude, but its variation shows the similar tendency with the case of previous study.

Hydrolysis of Arabinoxylo-oligosaccharides by α-ʟ-Arabinofuranosidases and β-ᴅ-Xylosidase from Bifidobacterium dentium

  • Lee, Min-Jae;Kang, Yewon;Son, Byung Sam;Kim, Min-Jeong;Park, Tae Hyeon;Park, Damee;Kim, Tae-Jip
    • Journal of Microbiology and Biotechnology
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    • v.32 no.2
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    • pp.187-194
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    • 2022
  • Two α-ʟ-arabinofuranosidases (BfdABF1 and BfdABF3) and a β-ᴅ-xylosidase (BfdXYL2) genes were cloned from Bifidobacterium dentium ATCC 27679, and functionally expressed in E. coli BL21(DE3). BfdABF1 showed the highest activity in 50 mM sodium acetate buffer at pH 5.0 and 25℃. This exo-enzyme could hydrolyze p-nitrophenyl arabinofuranoside, arabino-oligosaccharides (AOS), arabinoxylo-oligosaccharides (AXOS) such as 32-α-ʟ-arabinofuranosyl-xylobiose (A3X), and 23-α-ʟ-arabinofuranosyl-xylotriose (A2XX), whereas hardly hydrolyzed polymeric substrates such as debranched arabinan and arabinoxylans. BfdABF1 is a typical exo-ABF with the higher specific activity on the oligomeric substrates than the polymers. It prefers to α-(1,2)-ʟ-arabinofuranosidic linkages compared to α-(1,3)-linkages. Especially, BfdABF1 could slowly hydrolyze 23,33-di-α-ʟ-arabinofuranosyl-xylotriose (A2+3XX). Meanwhile, BfdABF3 showed the highest activity in sodium acetate at pH 6.0 and 50℃, and it has the exclusively high activities on AXOS such as A3X and A2XX. BfdABF3 mainly catalyzes the removal of ʟ-arabinose side chains from various AXOS. BfdXYL2 exhibited the highest activity in sodium citrate at pH 5.0 and 55℃, and it specifically hydrolyzed p-nitrophenyl xylopyranoside and xylo-oligosaccharides (XOS). Also, BfdXYL2 could slowly hydrolyze AOS and AXOS such as A3X. Based on the detailed hydrolytic modes of action of three exo-hydrolases (BfdABF1, BfdABF3, and BfdXYL2) from Bf. dentium, their probable roles in the hemiceullose-utilization system of Bf. dentium are proposed in the present study. These intracellular exo-hydrolases can synergistically produce ʟ-arabinose and ᴅ-xylose from various AOS, XOS, and AXOS.

Wrinkle Improvement, Whitening Effect of Boseong Camellia sinensis Vaccum Distilled Extract and Antioxidant, Antibacterial Efficacy of its Hydrothermal Extract (보성녹차 감압증류추출물의 주름개선, 미백 효능과 열수추출물의 항산화, 항균효과)

  • Lee, Kwang Won;Hong, Jung Hyun;Chung, Sun Hwa;Kim, Young Kyun;Park, Shinsung;Park, Su In;Shin, Moon Sam
    • The Journal of the Convergence on Culture Technology
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    • v.7 no.4
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    • pp.845-855
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    • 2021
  • In this study, Camellia sinensis Leaf cultivated in Boseong was extracted by vacuum distillation and hydrothermal extraction methods, and we performed in vitro experiments such as antioxidant, anti-wrinkle, whitening, and antibacterial activity test and HPLC analysis. Wrinkle improvement (elastase inhibition assay) and whitening effect (mushroom tyrosinase inhibition assay) of Boseong vacuum distilled extract have excellent results and IC50 values of vacuum distillation extract was measured at a lower concentration than that of the hydrothermal extract. The antioxidant activity of the Boseong hydrothermal extract was excellently measured in DPPH radical and ABTS+ radical scavenging activity, SOD-like activity, and total polyphenol content. In the antibacterial experiment, the Boseong hydrothermal extract formed a clear zone for the Staphylococcus aureus and Cutibacterium acnes strain. As a result of HPLC analysis, EGCG and caffeine in the hydrothermal extract were 40.29 mg/g, 9.94 mg/g respectively, and caffeine in the vacuum distilled extract was 3.40 mg/g. Therefore, we examined that Boseong Camellia sinensis vacuum distilled extract has excellent anti-wrinkle and whitening effect, and its hydrothermal extract has good antioxidant and antibacterial efficacy.

Effects of Microbial Additives and Silo Density on Chemical Compositions, Fermentation Indices, and Aerobic Stability of Whole Crop Rice Silage (미생물 첨가와 사일로 밀도가 총체벼 사일리지의 영양소 함량, 발효특성 및 호기적 안전성에 미치는 영향)

  • Joo, Young Ho;Jeong, Seung Min;Seo, Myeong Ji;Lee, Seong Shin;Choi, Ki Choon;Kim, Sam Churl
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.42 no.2
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    • pp.96-102
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    • 2022
  • The present study investigated effects of microbial additives and silo density on chemical compositions, fermentation indices, and aerobic stability of whole crop rice (WCR) silage. The WCR ("Youngwoo") was harvested at 49.7% dry matter (DM), and ensiled into 500 kg bale silo with two different compaction pressures at 430 kgf (kilogram-force)/cm2 (LOW) and 760 kgf/cm2 (HIGH) densities. All WCR forage were applied distilled water (CON) or mixed inoculants (Lactobacillus brevis 5M2 and Lactobacillus buchneri 6M1) with 1:1 ratio at 1x105 colony forming unit/g (INO). The concentrations of DM, crude protein, ether extract, crude ash, neutral detergent fiber, and acid detergent fiber of whole crop rice before ensiling were 49.7, 9.59, 2.85, 6.74, 39.7, and 21.9%, respectively. Microbial additives and silo density did not affect the chemical compositions of WCR silage (p>0.05). The INO silages had lower lactate (p<0.001), but higher propionate (p<0.001). The LOW silages had higher lactate (p=0.004). The INO silages had higher yeast count (p<0.001) and aerobic stability (p<0.001). However, microbial counts and aerobic stability were not affected by silo density. Therefore, this study concluded that fermentation quality of WCR silage improved by microbial additives, but no effects by silo density.

Effect of Selected Inoculant Applications on Chemical Compositions and Fermentation Characteristics of High Moisture Rye Silage

  • Lee, Seong Shin;Jeong, Seung Min;Seo, Myeong Ji;Joo, Young Ho;Paradhipta, Dimas Hand Vidya;Seong, Pil Nam;Kim, Sam Churl
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.42 no.3
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    • pp.155-161
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    • 2022
  • The aim of this study was to investigate the effect of isolated lactic acid bacteria (LAB) on the quality of high moisture rye silage. Rye forage (Secale cereale L.) was harvested at the heading stage (27.3% of dry matter (DM)) and cut into approximately 3-5 cm lengths. Then, the forage divided into 4 treatments with different inoculants: 1) No additives (CON); 2) Lactobacillus brevis strain 100D8 at a 1.2 × 105 colony-forming unit (cfu)/g of fresh forage (LBR); 3) Leuconostoc holzapfelii strain 5H4 at a 1.0 × 105 cfu/g of fresh forage (LHO); and 4) Mixture of LBR and LHO (1:1 ratio) applied at a 1.0 × 105 cfu/g of fresh forage (MIX). About 3 kg of forage from each treatment was ensiled into a 20 L mini-bucket silo in quadruplicate for 100 days. After silo opening, silage was collected for analyses of chemical compositions, in vitro nutrient digestibilities, fermentation characteristics, and microbial enumerations. The CON silage had the highest concentrations of neutral detergent fiber and acid detergent fiber (p = 0.006; p = 0.008) and a lowest in vitro DM digestibility (p < 0.001). The pH was highest in CON silage, while lowest in LBR and MIX silages (p < 0.001). The concentrations of ammonia-N, lactate, and acetate were highest in LBR silage (p = 0.008; p < 0.001; p < 0.001). Propionate and butyrate concentrations were highest in CON silage (p = 0.004; p < 0.001). The LAB and yeast counts were higher in CON and LHO silages compare to LBR and MIX silages (p < 0.001). However, the mold did not detect in all treatments. Therefore, this study could conclude that L. brevis 100D8 and Leu. holzapfelii strain 5H4 can improve the digestibility and anti-fungal activity of high moisture rye silage.

Suppression of Coupled Pitch-Roll Motions using Quasi-Sliding Mode Control (준 슬라이딩 모드 제어를 이용한 선박의 종동요 및 횡동요 억제)

  • Lee, Sang-Do;Cuong, Truong Ngoc;Xu, Xiao;You, Sam-Sang
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.27 no.2
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    • pp.211-218
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    • 2021
  • This paper addressed the problems of controlling the coupled pitch-roll motions in a marine vessel exposed to the regular waves in the longitudinal and transversal directions. Stabilization of the pitch and roll motions can be regarded as the essential task to ensure the safety of a ship's navigation. One of the important features in the pitch-roll motions is the resonance phenomena, which result in unexpected large responses in terms of pitch and roll modes in some specific conditions. Besides, owing to its inherent characteristics of coupled combination and nonlinearity of restoring terms, the vessel shows various dynamical behaviors according to the system parameters, especially in the pitch responses. Above all, it can be seen that suppression of pitch rate remains the most significant challenge to overcome for ship maneuvering safety studies. To secure the stable upright condition, a quasi-sliding mode control scheme is employed to reduce the undesirable pitch and roll responses as well as chattering elimination. The Lyapunov theory is adopted to guarantee the closed stability of the pitch-roll system. Numerical simulations demonstrate the effectiveness of the control scheme. Finally, the control goals of state convergences and chattering reduction are effectively realized through the proposed control synthesis.

Characteristics of Static Loading and Dynamic Loading Tests for Bridge Capability (교량 내하력 평가를 위한 정적재하시험 및 동적재하시험 특성)

  • Lee, Sang Hun
    • Journal of the Society of Disaster Information
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    • v.16 no.4
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    • pp.638-649
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    • 2020
  • Purpose: The objective of this study is to evaluate the load carrying capacity of a target bridge structure based on the simple slab bridge of concrete over 20 years of public service. Method: By performing static loading test and dynamic loading test, the displacement, strain, impact factor, and natural frequency values were measured and evaluated through analysis method. Result: The main results of this study are as follows. First, the maximum displacement and maximum strain of S1 were assessed at 2.917 mm and 44.720 𝜇ε( tensile) and -13.760 𝜇ε(compression), respectively, with S2 maximum displacement and maximum strain being 2.100 mm and 4.870 𝜇ε(tensile), respectively. Second, the maximum measured impact factor was 0.191 in section S1 A-A, and the maximum measured impact factor was 0.155 in section S2 C-C. Third, the natural frequency was assessed at 6.086 Hz, and the measurement was found to be within the range of 6.152 Hz to 6.738 Hz. Conclusion: The tested bridge may be evaluated to show good behavior and characteristics for the design load.

Biochemical Properties of a Whitening Bioactive Agent Derived from Thrichoderma viridescens SW-1 (Trichoderma viridescens SW-1 미백 기능성소재의 생화학적 특성)

  • Kang, Dong Woo;Kim, Pan Gil;Kim, Sam Woong;Bang, Kyu Ho;Kim, Chul Ho;Lee, Sang Won;Gal, Sang Wan
    • Journal of Life Science
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    • v.31 no.7
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    • pp.654-661
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    • 2021
  • In this study, in order to isolate excellent whitening agents from fungal cultural broth, various fungi were collected from wild areas in South Korea and then screened for tyrosinase inhibition activity, as tyrosinase is a precursor for the biosynthesis of melanin in regulating skin color. A fungus strain that inhibits tyrosinase activity has been identified and confirmed as Trichoderma viridescens (later renamed T. viridescens SW-1) via ITS sequencing. In T. viridescens SW-1, tyrosinase inhibitory activity was strongest on day three of culture. A 5% culture broth showed a tyrosinase inhibitory activity greater than 90% and exhibited high thermostability on day three. At 10% culture broth, the accumulations of intra- and extracellular melanin were inhibited above 27.1% and 7.5%, respectively. In summary, the physical and functional properties of the tyrosinase inhibitory substances of T. viridescens SW-1 included high levels of inhibition of melanin synthesis and antioxidative activity as well as thermostability. Therefore, we suggest that the whitening substance identified from the cultural broth of T. viridescens SW-1 has potential for application as a functional cosmetic ingredient.