The Journal of the Convergence on Culture Technology (문화기술의 융합)

The International Promotion Agency of Culture Technology (국제문화기술진흥원)
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Wrinkle Improvement, Whitening Effect of Boseong Camellia sinensis Vaccum Distilled Extract and Antioxidant, Antibacterial Efficacy of its Hydrothermal Extract

보성녹차 감압증류추출물의 주름개선, 미백 효능과 열수추출물의 항산화, 항균효과

  • Received : 2021.10.27
  • Accepted : 2021.11.04
  • Published : 2021.11.30
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Abstract

In this study, Camellia sinensis Leaf cultivated in Boseong was extracted by vacuum distillation and hydrothermal extraction methods, and we performed in vitro experiments such as antioxidant, anti-wrinkle, whitening, and antibacterial activity test and HPLC analysis. Wrinkle improvement (elastase inhibition assay) and whitening effect (mushroom tyrosinase inhibition assay) of Boseong vacuum distilled extract have excellent results and IC50 values of vacuum distillation extract was measured at a lower concentration than that of the hydrothermal extract. The antioxidant activity of the Boseong hydrothermal extract was excellently measured in DPPH radical and ABTS+ radical scavenging activity, SOD-like activity, and total polyphenol content. In the antibacterial experiment, the Boseong hydrothermal extract formed a clear zone for the Staphylococcus aureus and Cutibacterium acnes strain. As a result of HPLC analysis, EGCG and caffeine in the hydrothermal extract were 40.29 mg/g, 9.94 mg/g respectively, and caffeine in the vacuum distilled extract was 3.40 mg/g. Therefore, we examined that Boseong Camellia sinensis vacuum distilled extract has excellent anti-wrinkle and whitening effect, and its hydrothermal extract has good antioxidant and antibacterial efficacy.

본 연구는 보성에서 재배된 녹차잎을 감압증류와 열수추출법으로 추출하여 항산화, 항주름, 미백, 항균 in vitro 실험과 HPLC 분석을 수행하였다. 보성녹차 감압증류추출물은 주름개선(Elastase 활성 저해 실험)과, 미백(Mushroom tyrosinase 활성 저해 실험) 효능에서 우수한 결과를 나타내었으며, 열수추출물보다 낮은 농도에서 IC50 값이 측정되었다. 보성녹차 열수추출물은 항산화 시험(DPPH, ABTS+ radical 소거능, SOD 유사활성능, 총 폴리페놀 함량)에서 우수한 결과를 나타내었다. 항균 실험에서는 보성녹차 열수추출물에 의해 Staphylococcus aureus, Cutibacterium acnes 균주에 대하여 Clear zone이 형성되었다. HPLC 분석 결과로, EGCG와 Caffeine이 열수추출물에서 40.29 mg/g, 9.94 mg/g 검출되었고, 감압증류추출물에서는 Caffeine이 3.4 mg/g 검출되었다. 따라서 보성녹차 감압증류추출물은 주름개선과 미백효과가 탁월하며, 보성녹차 열수추출물은 항산화, 항균효과가 우수함을 확인하였다.

Keywords

Acknowledgement

본 연구는 2017년도 과기정통부의 재원으로 한국연구재단 바이오·의료기기개발사업의 지원을 받아 수행되었음 (No. 2017M3A9D8048416).

References

  1. M. G. Kim, S. I. Park, G. M. An, S. H. Heo, and M. S. Shin, "Physiological Activity of Supercritical Poria cocos back Extract and Its Skin Delivery Application using Epidermal Penetrating Peptide," Journal of the Korean Applied Science and Technology(KOCS), Vol. 36, No. 3, pp. 766-778, September 2019. DOI: http://dx.doi.org/10.12925/jkocs.2019.36.3.766
  2. Y. Yoo, "Air pollution and childhood allergic disease," Allergy Asthma Respir Dis(AARD), Vol. 4, No. 4, pp. 248-256, July 2016. DOI: http://dx.doi.org/10.4168/aard.2016.4.4.248
  3. H. S. Byun, Y. S. Um, I. H. Hur, S. Y. Sim, and K. J. Kim, "The Quality of Life of Skin Disease Patients, Particularly Acne Patients," Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology(JKOOD), Vol. 19, No. 1, pp. 65-75, April 2006. DOI: http://dx.doi.org/10.6114/jkood.2014.27.3.026
  4. B. G. Lee, J. H. Kim, S. G. Ham, and C. E. Lee, "Study on Biological Activities of Extracts for Cosmeceutical Development from Lagerstroemia indica L. Branch," Korean Journal of Plant Resources(KJPR), Vol. 27, No. 1, pp. 029-034, February 2014. DOI: https://doi.org/10.7732/kjpr.2014.27.1.029
  5. K. H. Shim, H. S. Young, T. W. Lee, and J. S. Choi, "Studies on the Chemical Components and Antioxidative Effect of Solanum lyratum Thunb," Korean Journal of Pharmacognosy(KSP), Vol. 26, No. 2, pp. 130-138, June 1995.
  6. H. J. Lee, and S. N. Park, "Antioxidative Effect and Active Component Analysis of Quercus salicina Blume Extracts," Journal of Society of Cosmetic Scientists of Korea(SCSK), Vol. 37, No. 2, pp. 143-152, June 2011. DOI: https://doi.org/10.15230/SCSK.2011.37.2.143
  7. S. I. Park, G. M. An, M. G. Kim, S. H. Heo, and M. S. Shin, "Physiological Activity of Magnolia kobus A. P. DC. Leaf Extracts and Enhancement of Skin Permeation Using PCL-PEG Polymer Micelle and Cell Penetrating Peptide," Journal of Investigative Cosmetology (KSIC), Vol. 15, No. 3, pp. 255-262, September 2019. DOI: https://doi.org/10.15810/jic.2019.15.3.004
  8. B. S. Hwang, Y. S. Jeong, S. M. Oh, G. C. Kim, Y. S. Cho, and I. G. Hwang, "Catechin Contents of Green Tea Produced in Korea", The korean Society of Food Science and Nutrition(JKSFSN), Vol. 47, No. 8, pp. 857-862, August 2018. DOI: https://doi.org/10.3746/jkfn.2018.47.8.857
  9. J. H. Kang, Y. K. Park, S. T. Chung, and K. H. Row, "Extraction and Purification of EGCG(Epigallocatechin Gallate) from Green Tea," The Korean Society for Biotechnology and Bioengineering(KSBB), Vol. 14, No. 5, pp. 517-522, October 1999.
  10. S. H. Kim, D. S. H, and J. D. Park, "Changes of Some Chemical Compounds of Korean (Posong) Green Tea according to Harvest Periods," Korean Journal of Food Science and Technology(KJFST), Vol. 36, No. 4, pp. 542-546, August 2004.
  11. Z. Wu, L. Xie, Y. Li, Y. Wang, X. Wang, N. Wan, X. Huang, X. Zhang, and M. Yang, "A novel application of the vacuum distillation technology in extracting Origanum vulgare L. essential oils," Industrial Crops & Products(Ind. Crops. Prod.), Vol. 139, No. 32, pp. 1-8, November 2019. DOI: https://doi.org/10.1016/j.indcrop.2019.111516
  12. M. A. Falcao, A. L. B. Fianco, A. M. Lucas, M. A. A. Pereira, F. C. Torres, R. M. F. Vargas, and E. Cassel, "Determination of antibacterial activity of vacuum distillation fractions of lemongrass essential oil," Phytochemistry reviews(Phytochem. Rev.), Vol. 11, No. 4, pp. 405-412, September 2012. DOI: https://doi.org/10.1007/s11101-012-9255-3
  13. K. H. Musa, A. Abdullah, B. Kuswandi, and M. A. Hidayat, "A novel high throughput method based on the DPPH dry reagent array for determination of antioxidant activity," Food Chemistry(Food Chem.), Vol. 141, No. 4, pp. 4102-4106, December 2013. DOI: https://doi.org/10.1016/j.foodchem.2013.06.112
  14. O. P. Sharma, and T. K. Bhat, "DPPH antioxidant assay revisited," Food Chemistry (Food Chem.), Vol. 113, No. 4, pp. 1202-1205, April 2009. DOI: https://doi.org/10.1016/j.foodchem.2008.08.008
  15. S. B. Kedare, and R. P. Singh, "Genesis and development of DPPH method of antioxidant assay," Association of Food Scientists & Technologists (India)(AFST(I)), Vol. 48, No. 4, pp. 412-422, July-August 2011. DOI: https://doi.org/10.1007/s13197-011-0251-1
  16. K. Sirivibulkovit, S. Nouanthavong, and Y. Sameenoi, "Paper-based DPPH Assay for Antioxidant Activity Analysis," The Japan Society for Analytical Chemistry(JSAC), Vol. 34, No. 7, pp. 795-800, July 2018. DOI: https://doi.org/10.2116/analsci.18P014
  17. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, and Riceevans C. "Antioxidant activity applying an improved ABTS radical cation decolorization assay," Free Radical Biology & Medicine(FRBM), Vol. 26, No. 9/10, pp. 1231-1237, May 1999. https://doi.org/10.1016/S0891-5849(98)00315-3
  18. S. MARKLUND, and G. MARKLUND, "Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase," European Journal of Biochemistry(EJB), Vol. 47, No. 3, pp. 469-474, September 1974. DOI: https://doi.org/10.1111/j.1432-1033.1974.tb03714.x
  19. A. R. Kim, "Applied study of functional cosmetics regarding anti-oxidants, the anti-wrinkles and the anti-bacterial effect of coffee grounds extract," Master's Thesis Dissertation, Eulji University, Jung-gu, Daejeon, Republic of Korea, 2016.
  20. M. J. Kim, and E. J. Park, "Feature Analysis of Different In Vitro Antioxidant Capacity Assays and Their Application to Fruit and Vegetable Sample," Journal of the Korean Society of Food Science and Nutrition (JKSFSN), Vol. 40, No. 7, pp. 1053-1062, July 2011. DOI: http://dx.doi.org/10.3746/jkfn.2011.40.7.1053
  21. O. VALLISUTA, V. NUKOOLKARN, A. MITREVEJ, N. SARISUTA, P. LEELAPORNPI SID, A. PHRUTIVORAPONGKUL, and N. SIN CHAIPANID, "In vitro studies on the cytotoxicity, and elastase and tyrosinase inhibitory activities of marigold (Tagetes erecta L.) flower extracts," Experimental and Therapeutic Medicine(Exp. Ther. Med.), Vol. 7, No. 1, pp. 246-250, October 2014. DOI: https://doi.org/10.3892/etm.2013.1373
  22. M. O. Ko, H. J. Kang, J. H. Hwang, and K. W. Yang. "Screening of the antibacterial effects by ethanol extracts from natural plant in Jeju against Propionibacterium acnes," Journal of Society of Cosmetic Scientists of Korea(SCSK), Vol. 44, No. 1, pp. 59-66. March 2018. DOI: https://doi.org/10.15230/SCSK.2018.44.1.59
  23. T. Theppakorn, and S. Wongsakul, "Optimization and Validation of the HPLC-Based Method for the Analysis of Gallic acid, Caffeine and 5 Catechins in Green Tea," Naresuan University Journal(NUJST), Vol. 20, No. 2, pp. 1-11, May-August 2012.
  24. G. M. An, "Epidermal Permeation Experiment and Cosmetic Material Development using Polymer Micelle (PCL-PEG) Formulation of Red pinus densiflora and Petroselinum crispum extract," Master's Thesis Dissertation, Eulji University, Jung-gu, Daejeon, Republic of Korea, 2020.
  25. C. I. Park, and G. H. Park, "Antioxidant and Anti-wrinkling Effects of Extracts from Nelumbo nucifera leaves," The Korea journal of herbology(Kor. J. Herbol.), Vol. 31, No. 4, pp. 53-60, July 2016. DOI: http://dx.doi.org/10.6116/kjh.2016.31.4.53.
  26. M. G. Kim, "A Study on physiological activity and percutaneous permeability Enhancement of Supercritical Centella asiatica Extract obtained by optimal temperature process," Master's Thesis Dissertation, Eulji University, Jung-gu, Daejeon, Republic of Korea, 2020.
  27. I. Jasprica, M. Bojic, A. Mornar, E. Besic, K. Bucan, and M. Medic-Saric, "Evaluation of Antioxidative Activity of Croatian Propoli Samples Using DPPH· and ABTS·+ Stable Free Radical Assays," Molecules(Mol.), Vol. 12, No. 5, pp. 1006-1021, May 2007. DOI: http://dx.doi.org/10.3390/12051006
  28. C. H. Jeong, S. T. Kang, O. S. Joo, S. C. Lee, Y. H. Shin, K. H. Shim, S. H. Cho, S. G. Choi, and H. J. Heo, "Phenolic Content, Antioxidant Effect and Acetylcholinesterase Inhibitory Activity of Korean Commercial Green, Puer, Oolong, and Black Teas," The Korean Society of Food Preservation((KoSFoP), Vol. 18, No. 2, pp. 230-237, April 2009.
  29. E. J. Kim, J. Y. Choi, M. R. Yu, M. Y. Kim, S. H. Lee, and B. H. Lee, "Total Polyphenols, Total Flavonoid Contents, and Antioxidant Activity of Korean Natural and Medicinal Plants," Korean Journal of Food Science and Technology(KJFST), Vol. 44, No. 3, pp. 337-342, June 2012. DOI: https://doi.org/10.9721/KJFST.2012.44.3.337
  30. J. O. Kim, M. J Jung, H. J. Choi, J. T. Lee, A. K. Lim, J. H. Hong, and D. I. Kim, "Antioxidative and Biological Activity of Hot Water and Ethanol Extracts from Phellinus linteus," Journal of the Korean Society of Food Science and Nutrition(JKSFSN), Vol. 37, No. 6, pp. 684-690, June 2008. DOI: http://dx.doi.org/10.3746/jkfn.2008.37.6.684
  31. H. J. Lee, and S. N. Park, "Antioxidative Effect and Active Component Analysis of Quercus salicina Blume Extracts," Journal of the Society of Cosmetic Scientists of Korea(SCSK), Vol. 37, No. 2, pp. 143-152, June 2011. DOI: https://doi.org/10.15230/SCSK.2011.37.2.143
  32. A. Adhikari, H. P. Devkota, A. Takano, K. Masuda, T. Nakane, P. Basnet, and N. Skalko-Basnet, "Screening of Nepalese crude drugs traditionally used to treat hyperpigmentation: in vitro tyrosinase inhibition," International Journal of Cosmetic Science(IJSC), Vol. 30, No. 5, pp. 353-360, October 2008. DOI: https://doi.org/10.1111/j.1468-2494.2008.00463.x
  33. L. P. Xie, Q. X. Chen, H. Huang, H. Z. Wang, and R. Q. Zhang, "Inhibitory Effects of Some Flavonoids on the Activity of Mushroom Tyrosinase," Biochemistry(Moscow)(Biochem. (Mosc.)), Vol. 68, No. 4, pp. 487-491, April 2003. DOI: https://doi.org/10.1023/a:1023620501702
  34. N. Korkmaz, S. O. Sener, S. Akkaya, M. Badem, R. Aliyazicioglu, M. Abudayyak, E. Oztas, and U. Ozgen, "Investigation of antioxidant, cytotoxic, tyrosinase inhibitory activities, and phenolic profiles of green, white, and black teas," Turkish Journal of Biochemistry(TJB), Vol. 44, No. 33, pp. 278-288, July 2019. DOI: https://doi.org/10.1515/tjb-2017-0345
  35. J. J. BIEMER, "Antimicrobial Susceptibility Testing by the Kirby-Bauer Disc Diffusion Method," Annals of Clinical and Laboratory Science(ACLS), Vol. 3, No. 2, pp. 135-140, March-April 1973.