• Journal of Pharmaceutical Investigation
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• v.37 no.5
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• pp.315-321
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• 2007

The purpose of the present study was to evaluate the bioequivalence of two lercanidipine hydrochloride tablets, Zanidip tablet (LG Life Sciences Ltd., Korea, reference drug) and Samchundang Lercanidipine tablet 10 mg (Sam Chun Dang Pharm. Co. Ltd., Korea, test drug), according to the guidelines of Korea Food and Drug Administration (KFDA). After adding an internal standard (amlodipine maleate) to human serum, serum samples were extracted using hexan-isoamyl alcohol (100:1, v/v). Compounds were analyzed by liquid chromatography/tandem mass spectrometry. This method showed linear response over the concentration range of 0.05-20 ng/mL with correlation coefficient of 0.9999. The lower limit of quantitation using 0.5 mL of serum was 0.05 ng/mL which was sensitive enough for pharmacokinetic studies. Thirty healthy male Korean volunteers received each medicine at the lercanidipine hydrochloride dose of 20 mg in a $2\;{\times}\;2$ crossover study. There was a one-week washout period between the doses. Serum concentrations of lercanidipine were monitored by an LC/MS/MS fer over a period of 24 hr after the administration. $AUC_t$ (the area under the serum concentration-time curve from time 0 to 24 hr) was calculated by the linear trapezoidal rule method. $C_{max}$ (the maximum serum drug concentration) and $T_{max}$ (the time to reach $C_{max}$) were compiled from the serum concentration-time data. Analysis of variance was carried out using logarithmically transformed $AUC_t$ and $C_{max}$. No significant sequence effect was found for all of the bioavailability parameters, indicating that the crossover design was properly performed. The 90% confidence intervals of the $AUC_t$ ratio and the $C_{max}$ ratio for Samchundang Lercanidipine/Zanidip were log 0.9505-log 1.2258 and log 0.9987-log 1.2013, respectively. These values were within the acceptable bioequivalence intervals of log 0.80-log 1.25. Thus, the criteria of the KFDA guidelines for the bioequivalence was satisfied, indicating Samchundang Lercanidipine tablet 10 mg and Zanidip tablet are bioequivalent.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.24 no.1
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• pp.58-65
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• 2012

In this study, to reduce the motion of the vessels resulting from resonance and secondary undulation by long-period waves, numerical review on the control performance of resonator was carried out by attaching the resonator to the established harbor of real waters. In the numerical analysis, CGWAVE MODULE of commercial software SMS(Surface water Modeling System), a finite element model based on 2-dimensional elliptical mild slope equation was applied, and through comparative analysis of the existing experiments and analysis results on the rectangular model ports, the validity of the friction coefficients in which validity and effectiveness of SMS on the secondary undulation analysis is applied was verified. Based on this, the control performance of resonator was confirmed through comparative review of the secondary undulation according to whether or not to attach the resonator to rectangular harbor. In addition, to reduce long-period motion of the moored vessels and the secondary undulation which may occur in Pohang new port, the method to move the resonant period which causes abnormal motion of the vessels to long-term one was discussed through application of the resonators with various sizes, thereby identifying the availability.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.28 no.6
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• pp.361-374
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• 2016

When the seabed around and under gravity structures such as submerged breakwater is exposed to a large wave action long period, the excess pore pressure is generated significantly due to pore volume change associated with rearrangement soil grains. This effect leads a seabed liquefaction around and under structures as a result from decrease in the effective stress, and the possibility of structure failure is increased eventually. These facts shown above have been investigated in the previous studies related to regular and irregular waves. This study suggested a concrete mat for preventing the seabed liquefaction near the submerged breakwater. The concrete mat was mainly used as a countermeasure for scouring protection in riverbed. According to installation of the concrete mattress, the time and spatial series of the deformation of submerged breakwater, the pore water pressure, and the pore water pressure ratio in the seabed were investigated. Their results were also compared with those of the seabed unprotected with the concrete mat. The results presented were confirmed that the liquefaction potential of seabed under the concrete mattress is significantly reduced under regular wave field.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.28 no.6
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• pp.332-349
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• 2016

OLAFOAM is the powerful CFD code and is an expanded version of $OpenFOAM^{(R)}$, for wave mechanics simulation. The $OpenFOAM^{(R)}$ does provide many solvers to correspond to each object of the numerical calculation in a variety of fields. OLAFOAM's governing equation bases on VARANS (Volume-Averaged Reynolds-Averaged Navier-Stokes) equation, and the finite volume method is applied to numerical techniques. The program is coded in C++ and run on the Linux operating system. First of all, in this study, OLAFOAM was validated for 1) wave transformation inside porous structure under bore and regular wave conditions, 2) wave transformation by submerged breakwater under regular wave condition, and 3) regular wave transformation and resultant vertical velocity distribution under current by comparison with existing laboratory measurements. Hereafter, this study, which is almost no examination carried out until now, analyzed closely variation characteristics of water surface level, wave height, frequency spectrum, breaking waves, averaged velocity and turbulent kinetic energy around porous submerged breakwater in the wave and current coexisting field for the case of permeable or impermeable rear beach. It was revealed that the wave height fluctuation according to current direction(following or opposing) was closely related to the turbulent kinetic energy, and others.

• Korean Journal of Clinical Laboratory Science
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• v.36 no.2
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• pp.110-114
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• 2004

Apolipoprotein E is the major lipid-carrier protein in the brain, and several studies provided evidence that apolipoprotein E(ApoE) epsilon4 allele can be considered a genetic risk factor for Alzheimer's disease(AD). Inheritance of the APOE gene has three alleles: ${\varepsilon}2$, ${\varepsilon}3$ and ${\varepsilon}4$. There are six possible genotypes: ${\varepsilon}2/{\varepsilon}2$, ${\varepsilon}3/{\varepsilon}3$, ${\varepsilon}4/{\varepsilon}4$, ${\varepsilon}2/{\varepsilon}3$, ${\varepsilon}2/{\varepsilon}4$, ${\varepsilon}3/{\varepsilon}4$. AD is characterized by a progressive loss of function and death of nerve cells in several areas of the brain. The ${\varepsilon}4$ allele is associated with a risk for developing AD. People with the ${\varepsilon}4/{\varepsilon}4$ genotype have the highest risk, but people with the ${\varepsilon}2/{\varepsilon}4$ or ${\varepsilon}3/{\varepsilon}4$ genotypes are also likely to develop the disease. 64.3% of people carry the is ${\varepsilon}3/{\varepsilon}3$ genotype, 22.1% carry the second ${\varepsilon}3/{\varepsilon}4$ genotype but, ${\varepsilon}2/{\varepsilon}2$ genotype is not usually found of people carry the 3.6% is ${\varepsilon}4/{\varepsilon}4$ genotype in a total of a test group of 140 people. The ratio of ${\varepsilon}4/{\varepsilon}4$ genotype related directly with AD is less than the ${\varepsilon}3/{\varepsilon}3$ genotype, but the ${\varepsilon}2/{\varepsilon}4$ and ${\varepsilon}3/{\varepsilon}4$ genotype ratio of indirect AD risk is 25.7% in the group of people, regardless. Thus, we have referred to the benefit from the treatment of AD through apoE genotype diagnosis.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.48 no.4
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• pp.322-336
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• 2012

To study the catching efficiency of biodegradable trammel net for swimming crab (Portunus trituberculatus), three types (biodegradable, monofilament and multifilament) of trammel nets were used in the field test, and the tests were carried out 16 times with two different mesh sizes (105mm and 160mm) in the Yeonpyeong fishing ground of Korea, 2009~2011. The catching efficiency of three type nets was analyzed by catch in number, catch in weight and average weight per individual of small and large size swimming crab by net types and mesh sizes. Statistical T-test was also carried out to verify the efficiency between the three types of nets. The results are as follows. The catch in number of swimming crab was 24,667 and formed about 81.0% of total catch. Of all swimming crab catch, small swimming crabs with less than 64mm in carapace length which is a prohibited landing size by law formed 48.1%, larger swimming crabs with more than 64mm in carapace length which is a landing size formed 51.9%. In 105mm mesh size trammel net test, the catch share in number of small size swimming crab by biodegradable trammel net was 47.5% in comparison with multifilament trammel net and 74.2% in comparison with monofilament trammel net, so biodegradable trammel net has more protective effects on small size swimming crab than other types of trammel nets. The protective effects for small size swimming crab by biodegradable trammel net was 25.8~52.5% in comparison with other types of trammel nets. The catch share in weight of large size swimming crab by biodegradable trammel net was 98.3% in comparison with multifilament trammel net and 92.3% in comparison with monofilament trammel net, so biodegradable trammel net has a similar catch efficiency to multifilament trammel net. The results of 160mm mesh size trammel net test have shown similar results of 105mm mesh size trammel net test. This study shows that biodegradable trammel net is a more useful fishing gear than multifilament and monofilament trammel net because biodegradable trammel net has lower catch rate than other types of nets in small size swimming crab and similar catch rate than multifilament trammel net which is a well used net by fishermen.

• Horticultural Science & Technology
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• v.28 no.6
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• pp.942-947
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• 2010

In the present study, pollen tube growth and fruit set in Asian pear ($Pyrus$ $pyrifolia$) were examined upon the mechanical damage on the stigma and style following the artificial pollination. When the upper middle part of style was cut off at 48 h after the artificial pollination, pollen tube grew to the middle of the style and the subsequent pollen tube elongation and fruit set normally occurred. When the style was cut off before the pollen tube reached the middle of the style, however, no pollen tube elongation and fruit set occurred. With the pollen tube elongation through the style, callose plugs at the basal part of the pollen tube were formed to the direction of the elongation at regular intervals. When the upper part of pollen tube attached pollen grain was cut off, however, callose plugs were formed defectively in the pollen tube, and the pollen tube opened and lost their turgor pressure. These facts might be the reason for the ceased or delayed pollen tube elongation and ultimately the failure of the fertilization.

• Korean Journal of Food Science and Technology
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• v.44 no.2
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• pp.216-223
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• 2012

Dextran was produced by $Leuconostoc$ $mesenteroides$ SM with various contents of yeast extract and its rheological properties were modulated, via an addition of hydroxypropyl methylcellulose (HPMC) of different viscosities. The conversion yield reached 90% after adding 3% yeast extract, which remained constant, thereafter. The acidity of the cultures was approximately 1.4 and 0.9% after fermentation for 24 h at 25 and $30^{\circ}C$, respectively. The total dextran content (107.3 g/kg) was the highest in the presence of the 3% yeast extract. Under the same conditions, the consistency, viscous modulus (G"), and elastic modulus (G') of the cultures were $37.6\;Pa{\cdot}s^n$, 38 Pa, and 50 Pa, respectively. The rheological properties of the culture were changed drastically by the fortification with HPMC of higher concentration and viscosity. The addition of 10% HPMC (4,000 cp) resulted in a significant increase in G" to 1,950 Pa. Furthermore, adding HPMC to a viscous culture resulted in a remarkable increase in both hardness and firmness.

• Korean Journal of Food Science and Technology
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• v.46 no.2
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• pp.165-172
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• 2014

Mixed fermentation of cow milk was performed by sequential co-cultures with Bacillus subtilis and Lactococcus lactis. After a first fermentation step with B. subtilis for 6 h, the number of viable cells increased to $2.5{\times}10^8$ CFU/mL. The second fermentation step with L. lactis resulted in increased viable cells $1.09{\times}10^{10}$ CFU/mL for 3 days and increased acidity. However, the number of viable B. subtilis cells was decreased greatly to $5{\times}10^1$ CFU/mL following fermentation with L. lactis. Milk proteins were markedly hydrolyzed by the first fermentation after 2 h, and the second fermentation induced curd formation in milk. However, after 4 h, the first fermentation resulted in higher whey separation and 80 mg% tyrosine content. Gamma-aminobutyric acid (GABA) production was dependent upon the degree of protein hydrolysis by first fermentation. Second fermentation resulted in 0.14% GABA. The milk fermented by B. subtilis indicated the rough surface of yogurt depended upon the degree of protein hydrolysis. In conclusion, set-type yogurt was efficiently produced by co-culturing of milk, and fortifying with peptides, GABA, and probiotics.

• Korean Journal of Food Science and Technology
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• v.45 no.4
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• pp.434-440
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• 2013

Functional properties of Cheonnyuncho fruit paste (CFP) were enhanced by fermentation, using Leuconostoc mesenteroides SM. The stability of CFP pigment was maintained and microorganisms in crude CFP were sterilized by heating at $80^{\circ}C$ for 30 min. The production of dextran in CFP was increased by increasing the fermentation time and sugar content, resulting in an increase of consistency. The CFP fermented with 20% sucrose contained 4.8% mucilage after fermentation for 2 days. In particular, the production of mucilage was greatly enhanced by fortification with 3% sodium citrate in CFP, resulting in mucilage production of 7%. In addition, the viscous modulus of fermented CFP showed a greater increase than that of the elastic modulus. Thus, it was concluded that CFP fermented by L. mesenteroides SM can be fortified with functional ingredients, such as mucilage and probiotics, with enhanced rheological properties. Therefore fermented CFP can be utilized as the functional ingredients for functional foods.