• Journal of Life Science
• /
• v.16 no.5
• /
• pp.723-728
• /
• 2006

The species of Grateloupia filicina, Grateloupia divaricata in East Sea were investigated taxonmically in order to clarify taxonomic position. The ecological character, external morphology, anatomy of vegetative structure. Blade length are $15{\sim}40\;cm$, erect from discoidal holdfast of $3{\sim}10\;mm$ in diameter. Stipe $1{\sim}2.5\;cm$ long, narrowly cylindrical below, compressed above Grateloupia filicina. Main axis are long and compressed, $3{\sim}7\;mm$ broad in broadest part. Colors are scarlet to light red. Blade length are $10{\sim}25\;cm$, erect from discoidal holdfast of $3{\sim}8\;cm$ in diameter Grateloupia divaricata. Stipe are single and simple $2{\sim}5\;mm$ broad. Thallus composed of cortex and medulla in section ; cortex composed of $9{\sim}10$ layers of anticlinally arranged cortical cell, divided into outer, middle and inner parts. Partial fragments of nuclear 18S rDNAs from the two species of Grateloupia (Rhodophyta) were amplified using the PCR reaction and sequenced to compare their similarity. The partial sequences showed 98.9% similarity each other. Grateloupia filicina has 371 bp sizes and Grateloupia divaricata has 372 bp size. The G+C contents of Grateloupia filicina is 54.3% and Grateloupia divaricata is 53.64%.

• Journal of Food Hygiene and Safety
• /
• v.24 no.4
• /
• pp.385-390
• /
• 2009

This survey was conducted to monitor the total sugar, sodium, and artificial sweetener contents of light meals from the school zone in Gwangju, from November, 2008 to April, 2009. A total of 100 samples were tested. HPLC/ELSD was used for the determination of total sugar contents and AAS for sodium contents. Total sugar amounts were the sum of both mono-and disaccharide according to nutritional information standard defined by the Korea Food and Drug Administration. The results were as follows by the form of total sugar content(sodium content): $5.7\;{\pm}\;1.9%$($4.6\;{\pm}\;1.6\;mg/g$) for Tteokbokki, $7.6\;{\pm}\;5.1%$($5.4\;{\pm}\;2.7\;mg/g$) for Chicken(skewed), $15.4\;{\pm}\;3.2%$($3.0\;{\pm}\;0.9\;mg/g$) for Bun(fish-shaped), $0.6\;{\pm}\;0.3%$($3.7\;{\pm}\;1.0\;mg/g$) for Sundae, $0.9\;{\pm}\;0.3%$($7.4\;{\pm}\;1.4\;mg/g$) for Oden(with broth), $20.5\;{\pm}\;6.2%$($2.9\;{\pm}\;0.6\;mg/g$) for Waffle, $6.8\;{\pm}\;2.2%$($4.7\;{\pm}\;1.0\;mg/g$) for Hotdog, $14.2\;{\pm}\;2.8%$($3.1\;{\pm}\;1.6\;mg/g$) for Hotteok, $6.6\;{\pm}\;2.1%$($3.9\;{\pm}\;0.6\;mg/g$) for Toast, $10.1\;{\pm}\;3.7%$($2.3\;{\pm}\;0.7\;mg/g$) for Fry(sweet potato), $1.6\;{\pm}\;0.7%$($4.0\;{\pm}\;0.7\;mg/g$) for Fry(etc), and $9.3\;{\pm}\;2.4%$($4.0\;{\pm}\;0.6\;mg/g$) for Doughnut. In addition, sodium saccharin, one of the artificial sweeteners, was tested. Among 60 samples from February to April, 2009, 11 samples had sodium saccharin(6.7~101.0 mg/kg).

• Journal of the Korean Society of Fisheries and Ocean Technology
• /
• v.30 no.3
• /
• pp.172-181
• /
• 1994

The mesh selectivity of diamond and suare mesh cod-ends at the Southern Korean Sea and the East China Sea were compared for Pampus argenteus, Trachurus japonicus, Trichiurus lepturus. Selection trials were carried out using diamond and square mesh cod-end by trouser type cod-end with cover net. of which the mesh cod-end has four types : A(51.2mm), B(70.2mm), C(77.6mm), D(88.0mm). Selection curves and selection parameters were calculated using a logistic model. The results obained are summarized as follows : 1. Harvest fish : In B. C and D type selection range and fifty percent selection length of the square mesh were about 21mm, 11mm : 12mm, 18mm and 34mm, 5mm higher than those of the diamond mesh, respectively. Selection factor of master curve for the diamond mesh was 1.54 and for the square mesh was 1.68. The optimum mesh size for the diamond mesh was 97.4mm and for the square mesh was 89.3mm, the difference was 8.1mm. 2. Horse mackerel : In A type, selection range was nearly the same for the diamond and the square mesh, but fifty percent selection length of the square mesh was 43mm higher than the diamond mesh. In B. C and D type, selection range and fifty percent selection length of the square mesh were about 6mm, 3mm : 24mm, 21mm and 11mm, 42mm higher than those of the diamond mesh, respectively. Selection factor of master curve for the diamond mesh was 2.37, for the square mesh was 2.77. The optimum mesh size for the diamond mesh was 78.1mm and for the square mesh was 66.8mm, the difference was 11.3mm. 3. Hair tail : In A, B and C type, selection range of the square mesh was about 34mm, 8mm, 60mm higher than those of the diamond mesh. Fifty percent selection length for the diamond mesh was about 5mm, 7mm, 8mm higher than that of the square mesh. Selection factor of master curve for the diamond mesh was 3.11, for the square mesh was 3.48. The optimum mesh size for the diamond mesh was 64.3mm and for the square mesh was 57.5mm, the difference was 6.8mm.

• The Journal of the Korean Society for Microbiology
• /
• v.13 no.1
• /
• pp.1-5
• /
• 1978

Listeria monocytogenes infection was considered a rather rare disease and occurs mostly either in newborn babies or in young children. However, there has been increasing reports of this infection in elderly person with various underlying disease. Recently we have experienced two cases of Listeria meningitis; one in a 4-year-old male with an acute lymphoblastic leukemia, and the other in a 43-year-old female with a breast cancer. Both were on various chemotherapeutic agents for their primary diseases when the organism, L. monocytogenes was found in their celebospinal fluid(CSF). The degree of CSF pleocytosis were quite different by cases. The former case showed a marked increase, $3,350/mm^3$, and the latter slight, $410/mm^3$, Both showed a slight decrease of CSF glucose ranging 39 to 43mg/100ml. It seems that a routine CSF analysis bears a limitted value in the diagnosis or Listeria meningitis. A direct smear of CSF with Gram's stain revealed gram-positive bacilli in one case, but none in the other. Bacterial culture of CSF yielded plenty colonies in one case, but a few in the other. It seems that isolation of L. monocytogenes must not be considered very easy, and a negative direct smear does not necessarily mean a negative culture. The two isolates we obtained showed the typical cultural and biochemical characteristics of L. monocytogenes and were found to belong to serotypes 1b and 4b. It was our experience that the identification of this organism was not very much matter because of its distinct characteristics, but the most important matter was how to think of the possibility of this organism at the begining. The two isolates were both susceptible to cephalothin, chloramphenicol, erythromycin, tetracycline and gentamicin; intermediate to ampicillin, penicillin and kanamycin; and resistant to cloxacillin.

• Journal of the Korean Society of Fisheries and Ocean Technology
• /
• v.30 no.3
• /
• pp.161-171
• /
• 1994

Mesh selection analysis for the trawl net were carried out at the Southern Korean Sea and the East China Sea during the 1992-1994 years by the training ship Seabada of the National Fisheries University of Pusan, using A type (51.2mm), B type (70.2mm), C type (77.6mm), D type (88.0mm) square mesh cod-ends. The fishing trials were made using bottom trawl of the trouser type cod-end with cover net. Selection curves and selection parameters were calculated by a logistic model for Sphyraena pinguis, Pampus argenteus, Trachurus japonicus, Pagrus major, Callanthias japonicus, Trichiurus lepturus. The results obtained are summarized as follows: 1. Red barracuda : Selection range and fifty percent selection length in the A type was 115.8mm, 292.8mm, respectively. 2. Harvest fish : Each selection range and fifty percent selection length in the B. C. and D type was 37.7mm, 113.8mm : 40.1mm, 131.7mm and 64.8mm, 148.6mm, respectively. Selection parameters of master curve were : slope, 3.81 : intercept, -6.4. Optimum mesh size was 89.3mm. 3. Horse mackerel : Each selection range and fifty percent selection length in the A, B, C and D type was 43.0mm, 148.3mm : 60.7mm, 183.2mm, 214.5mm and 91.4mm, 254.9mm, respectively. Selection parameters of master curve were : slope 2.30 : intercept, -6.4. Optimum mesh size was 66.8mm. 4. Red seabrem : Selection range and fifty percent selection length in the D type was 42.7mm, 203.4mm, respectively. 5. Yellowsail red bass : Selection range and fifty percent selection length in the A type was 84.0mm, 110.6mm, respectively. 6. Hair tail : Each selection range and fifty percent selection length in the A, B and C type was 59.7mm, 176.0mm : 100.9mm, 250.7mm and 178.6mm, 307.0mm, respectively. Selection parameters of master curve were : slope, 1.54 : intercept, -5.4. Optimum mesh size was 57.5mm.

• The Journal of the Korean Society for Microbiology
• /
• v.35 no.2
• /
• pp.97-108
• /
• 2000

Two-dimensional gel electrophoresis (2-DE) is an essential tool of proteomics to analyse the entire set of proteins of an organism and its variation between organisms. Helicobacter pylori was tried to identify differences between strains. As the first step, whole H. pylori was lysed using high concentration urea contained lysis buffer [9.5 M Urea, 4% CHAPS, 35 mM Tris, 65 mM DTT, 0.01% SDS and 0.5% Ampholite (Bio-Rad, pH 3-10)]. The extract ($10\;{\mu}g$) was rehydrated to commercially available immobilised pH gradient (IPG) strips, then the proteins were separated according to their charges as the first dimensional separation. The IPG strips were placed on Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) to separate according to molecular mass of the proteins as the second dimension. The separated protein spots were visualised by silver staining in order to compare different expression of proteins between strains. Approximately 120 spots were identified in each mini-protein electrophoresised gel, furthermore about 65 to 75 spots were regarded as identical proteins in terms of pI value and molecular weight between strains used. In addition, distinct differences were found between strains, such as 219-1, Y7 and Y14, CH150. Two representative strains were examined using strips which had pH range from 4 to 7. This strips showed a number of isoforms which were considered large spots on pH range 3-10. Furthermore, the rest of spots on pH 4-7 IPG strips appeared very distinctive compared to broad range IPG strips. 2-DE seems to be an excellent tool for analysing and identifying variations between H. pylori strains.

• Applied Biological Chemistry
• /
• v.44 no.4
• /
• pp.262-268
• /
• 2001

Two commercial wood vinegar liquors prepared from Cryptomeria japonica and Quercus sp., which are used as a mineral fertilizer in Korea, were extracted using dichloromethane as a solvent, respectively. The extracts were separated into acidic, phenolic, neutral and basic fraction by acid or alkali treatment, and the compositions of each fraction were analyzed by means of GC and GC-MS. A total of 103 compounds including 26 acids, 32 phenols and 45 neutral compounds were identified. The major components were acetic, propionic and n-butyric acid, representing of $41{\sim}58%$ of the acidic fraction, guaiacol, 4-methylguaiacol and phenol, repersenting of $53.2{\sim}63.9%$ of the phenolic fraction, and furfural, 3-methyl-2-cyclopenten-2-one, 2,3-dimethyl-2-cyclopenten-1-one and 5-methyl-2-furfural in the neutral fraction. In addition to these compounds, phenolic fraction in dichloromathane extract from wood vinegar liquor of C. japonica included large amounts of vanillin, acetovanillone and tentatively identified ethylvanillyl ether while that of Quercus sp. included some amounts of syringol and 4-methylsyringol.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.14 no.4
• /
• pp.1576-1581
• /
• 2013

In the present study, the performance of a PF heat exchanger for heat pump dryer was investigated. Capacity and dehumidification amount of the PF heat exchangers(PF1, PF2, PF3) by different inclination angles($0^{\circ}$, $30^{\circ}$, $60^{\circ}$) were studied. Experimental conditions were an air velocity crossing to the heat exchanger(0.5m/s), an air dry-bulb temperature($60^{\circ}C$) and relative humidity(70%). The experimental results have shown that the performance of the inclined PF heat exchangers was better than that of the vertically installed one. PF3 showed better performance compared to PF1 and PF2 due to the large pin pitch which are leading to more draining for dehumidified water. But, capacity and dehumidification amount of the PF heat exchanger at the inclination angles of $60^{\circ}$ was decreased due to pressure drop. Also, to predict the experimental data of the PF heat exchanger, the performance program was developed for the inclination angles of $0^{\circ}$. PF heat exchanger performance between experiment data and calculation data was satisfied within the maximum 2% for capacity and 3% for dehumidification amount.

• Archives of Plastic Surgery
• /
• v.35 no.1
• /
• pp.36-41
• /
• 2008

Purpose: For facial reconstruction, skin color match is crucial to achieve great aesthetic result. Forehead flap and full thickness skin graft have been used for many years to reconstruct facial defect. Their results are aesthetically valuable with remarkable resemblance and harmony of the skin color between donor and recipient sites. The purpose of this study is to evaluate and compare the aesthetic outcome of the two methods as the analysis of skin color match. Methods: From January 1995 to December 2005, ten forehead flaps and ten full thickness skin grafts were performed. The reconstructed areas of forehead flaps were five noses and five eyelids. Recipient sites of full thickness skin grafts were seven eyelids, two noses and one forehead. In order to obtain the objective validity, the skin color of flap(or graft) and the recipient sites were measured by chromameter. The skin colors were quantified according to a three-dimensional coordinate system used in chromameter, L*(brightness), a*(redness), and b*(yellowness).Results: There was no significant color difference between forehead flap site and adjacent skin in all color values. On the other hand, the L* and b* values of graft sites were significantly lower than those of the adjacent skins. The a* values of graft sites were higher than those of the adjacent skins. Conclusion: This study reveals that skin color match of forehead flap is greater than that of full thickness skin graft. As forehead flap has adequate volume and great color match, it can be useful to reconstruct deep facial defect such as nasal defect. On the other hand, full thickness skin graft can be used for superficial defect like partial eyelid defect.

• Journal of radiological science and technology
• /
• v.38 no.4
• /
• pp.355-363
• /
• 2015

Advancement in the medical field provides an opportunity for the development of medical equipment and also enable accurate diagnosis for the inspection and the treatment of diseases. The aging of equipment due to the frequent operation produce uncertainty in the patient exposure dose, so a quality control was implemented by establishing a safety management system on a regular basis. The x-ray tube voltage (kVp), which is directly involved in the patient exposure dose and the life of the equipment, needs periodic performance for the quality control, but it has a limitation due to the time and the cost. In this study, we proposed a new method for measuring the kVp with ease by using the Y and Cu to solve the problem of the time and cost. We also evaluated the usefulness and the effectiveness of the new method and its accuracy through the kVp measurement. After securing the condition, the amount of the tube current and evaluating the usefulness and effectiveness of kVp measured using Y and Cu. The density range used was at 0.5-1.0, kVp was in the range of ${\pm}10%$, then, we recorded the change in concentration as % of Y and Cu. This experiment showed that when Cu and Y was at 75 kVp, concentration was the same, and when the kVp was changed to 65 ~ 85 %, a gradual increase in concentration to 85 ~ 110 % was noted.