• Journal of the Korea Institute of Military Science and Technology
• /
• v.18 no.4
• /
• pp.478-483
• /
• 2015

Decontamination of biological agents utilizes hydrogen peroxide($H_2O_2$) for its effectiveness and safeness. Bacillus anthracis is a major target for $H_2O_2$ decontamination. To assess the effect of $H_2O_2$ on B. anthracis and identify biomarkers for decontamination, whole transcriptomic profiling of $H_2O_2$-treated B. anthracis was performed. Here we identified deregulation in stress response genes, transcription factors and cellular homeostasis genes. We also found that expression of antisense RNAs increased in B. anthracis during decontamination. We postulate that B. anthracis prioritizes survival and adaptation in response to $H_2O_2$ treatment by changing its gene expression pattern.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
• /
• v.1
• /
• pp.9-14
• /
• 2006

This paper develops a complete methodology for the mitigation of ionosphere spatial anomalies by GBAS systems fielded in the Conterminous U.S. (CONUS). It defines an ionosphere anomaly threat model based on validated observations of unusual ionosphere events in CONUS impacting GBAS sites in the form of a linear ‘wave front’ of constant slope and velocity. It then develops a simulation-based methodology for selecting the worst-case ionosphere wave front impact impacting two satellites simultaneously for a given GBAS site and satellite geometry, taking into account the mitigating effects of code-carrier divergence monitoring within the GBAS ground station. The resulting maximum ionosphere error in vertical position (MIEV) is calculated and compared to a unique vertical alert limit, or $VAL_{H2,I}$, that applies to the special situation of worst-case ionosphere gradients. If MIEV exceeds $VAL_{H2,I}$ for one or more otherwise-usable subset geometries (i.e., geometries for which the 'normal' vertical protection level, or $VPL_{H0}$, is less than the 'normal' VAL), the broadcast ${\sigma}_{pr_{-}gnd}$ and/or ${\sigma}_{vig}$ must be increased such that all such potentially-threatening geometries have VPL$_{H0}$ > VAL and thus become unavailable. In addition to surveying all aspects of the methods used to generate the required ${\sigma}_{pr_{-}gnd}$ and ${\sigma}_{vig}$ inflation factors for CONUS GBAS sites, related methods for deriving similar results for GBAS sites outside CONUS are suggested.

• Journal of the Korea Institute of Information and Communication Engineering
• /
• v.13 no.12
• /
• pp.2633-2640
• /
• 2009

We design a small-area, low-power, and high-speed EEPROM for touch screen controller IC. As a small-area EEPROM design, a SSTC (side-wall selective transistor) cell is proposed, and high-voltage switching circuits repeated in the EEPROM core circuit are optimized. A digital data-bus sensing amplifier circuit is proposed as a low-power technology. For high speed, the distributed data-bus scheme is applied, and the driving voltage for both the EEPROM cell and the high-voltage switching circuits uses VDDP (=3.3V) which is higher than the logic voltage, VDD (=1.8V), using a dual power supply. The layout size of the designed 128-KBit EEPROMIP is $662.31{\mu}m{\times}1314.89{\mu}m$.

• The Transactions of the Korea Information Processing Society
• /
• v.4 no.7
• /
• pp.1665-1680
• /
• 1997

The Entity-Relationship (E-R) model is widely used not only to increase understanding between user and designer, but also to model the relationship of real world data appropriately when designing database system in many application areas. It should be then transformed into an Object-Oriented database model which gives good merits to represent and manipulate data efficiently. Therefore, a method of transforming an E-R model into an Object-Oriented database model should be studied, but without losing any semantics of concept for the E-R model. This paper not only deals with transformation rules taking as input the elements of E-R model and delivering the elements of an Object-Oriented database model, but also improves the concept of generalization and aggregation inheritance. The paper also presents a method of transformation of relationship depending on these rules. The proposed method that obtains Object-Oriented database schema from an E-R model with preserving the properties of the E-R model is shown with examples. The method presented is able to be used to the logical database design.

• Journal of the Korean Geosynthetics Society
• /
• v.3 no.1
• /
• pp.43-52
• /
• 2004

Laboratory model tests were conducted to assess the behavior characteristics of geogrid reinforced earth walls using fiber-mixed soil backfill with different surcharge loads and reinforcement spacing. The models were built in the box having dimensions, 100cm tall, 140cm long, and 100cm wide. The reinforcements used were geonet(tensile strength, 0.79t/m) and geogrid(tensile strength, 2.26t/m). Decomposed granite soil(ML) with or without polypropylene fiber was used backfill material. Strain gauges and LVDTs were installed on the retaining walls to measure the strain of the reinforcements and the displacements of the wall facings.

• Journal of Korean Society of Coastal and Ocean Engineers
• /
• v.23 no.3
• /
• pp.248-257
• /
• 2011

In the present study, we proposed a new numerical wave tank model to analyze the vertical tension-leg circular floating bodies, using a 2-D Navier-Stokes solver. An IBM(Immersed Boundary Method) capable of handling interactions between waves and moving structures with complex geometry on a standard regular Cartesian grid system is coupled to the VOF(Volume of Fluid) method for tracking the free surface. Present numerical results for the motions of the floating body were compared with existing experimental data as well as numerical results based on FAVOR(Fractional Area Volume Obstacle Representation) algorithm. For detailed examinations of the present model, the additional hydraulic experiments for floating motions and free surface transformations were conducted. Further, the versatility of the proposed numerical model was verified via the numerical and physical experiments for the general rectangular floating bodies. Numerical results were compared with experiments and good agreement was archived.

• Journal of Korean Society of Coastal and Ocean Engineers
• /
• v.30 no.4
• /
• pp.153-165
• /
• 2018

In this study, the performance of irregular wave field generation of olaFlow is first verified by comparing the frequency spectrum of the generated waves by the wave-source using olaFlow and the target wave. Based on the wave performance of irregular waves of olaFlow, the characteristics of the velocity field including the average flow velocity, longshore current and turbulent kinetic energy around the three-dimensional permeable submerged breakwaters, which act as the main external forces of the salient formation, are numerically investigated. The numerical results show that as the gap width between breakwaters decreases, the wave height in the center of the gap increases and as the gap width between breakwaters increases, the longshore currents become stronger. As a result, it is possible to understand the formation of the salient formed behind the submerged breakwaters.

• Textile Coloration and Finishing
• /
• v.8 no.3
• /
• pp.36-51
• /
• 1996

In order to investigate the degradation behavior of PET fabrics, sodium diethylene glycolate (SDEG)-diethylene glycol (DEG) solutions were prepared and PET fabrics were treated in the solution. The dissolution rate constant and apparent activation energy of the PET fabrics were calculated by Eyring's and Arrhenius's equation respectively and measured dyeing properties, moisture and antistatic properties. Then compared SDEG-treated fabrics with NaOH-treated. The results were as follows; 1. PET fabrics decreased their weight in SDEG-DEG solution, and the decreasing rate showed a linear relationship to the treating time at constant temperature and concentration of SDEG-DEG solution. 2. The dissolution rate constant showed a linear relationship to the concentration of SDEGDEG solution and an exponential relationship to treating temperature. 3. Apparent activation energy of dissolution was 23.45 kcal/mol. 4. The K/S values and the ΔL values of fabrics treated with SDEG-DEG solution are higher and lower respectively than fabrics treated with NaOH. 5. SDEG-DEG solution treatment improved fabric's moisture regain and it reached almost maximum at about 40% weight loss. 6. In the both reagent the light, wet and sublimation fastness of fabrics are similar. 7. SDEG-DEG solution gave more electrical discharge effect to the fabrics than that of NaOH. 8. NaOH treated PET microfiber have crater-like surface, while SDEG-DEG solution give bathochromic effect to the PET microfiber because which has wrinkles on the surface.

• Journal of Microbiology and Biotechnology
• /
• v.7 no.2
• /
• pp.144-150
• /
• 1997

We developed a simple and efficient method to prepare a Pseudomonas vaccine of outer membrane (OM) proteins free from lipopolysaccharide (LPS). A three step purification process including extraction, ultrafiltration and ultracentrifugation effectively removed LPS from the OM protein fraction. Approximately 2 mg of the OM proteins was obtained from 1 g of wet cell. LPS contaminant in the vaccine preparation was less than 0.003% (w/w) of protein and protease activity was not detectable. To achieve a wide range of protection, OM proteins prepared from four attenuated P. aeruginosa strains were mixed in equal amounts and used as a vaccine, which elicited in rabbits a high titer of antibody reactive to all of the seven Fisher types. The antisera from the immunized rabbit had a strong reactivity to vaccine proteins larger than 25 kDa. In a burned mouse infection model, immunization with the vaccine significantly enhanced bacterial clearance in the Pseudomonas infected skin. The vaccination also provided mice an excellent protection against Pseudomonas infection (11, 16). Data on antigenicity, mutagenicity, acute, subacute toxicity and pharmacological tests confirmed the safety of the vaccine (1, 3, 10, 12, 17). These data demonstrate that this method can be applied to manufacture a bacterial vaccine of OM proteins with safety and prophylactic efficacy at a practical low cost.

• Journal of Microbiology and Biotechnology
• /
• v.25 no.8
• /
• pp.1275-1280
• /
• 2015

Previously, we performed de novo RNA sequencing of Scolopendra subspinipes mutilans using high-throughput sequencing technology and identified several antimicrobial peptide candidates. Among them, a cationic antimicrobial peptide, scolopendrasin VII, was selected based on its physicochemical properties, such as length, charge, and isoelectric point. Here, we assessed the anticancer activities of scolopendrasin VII against U937 and Jurkat leukemia cell lines. The results showed that scolopendrasin VII decreased the viability of the leukemia cells in MTS assays. Furthermore, flow cytometric analysis and acridine orange/ethidium bromide staining revealed that scolopendrasin VII induced necrosis in the leukemia cells. Scolopendrasin VII-induced necrosis was mediated by specific interaction with phosphatidylserine, which is enriched in the membrane of cancer cells. Taken together, these data indicated that scolopendrasin VII induced necrotic cell death in leukemia cells, probably through interaction with phosphatidylserine. The results provide a useful anticancer peptide candidate and an efficient strategy for new anticancer peptide development.