• Title/Summary/Keyword: Lee Sam

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Inhibition of Metarhizium anisopliae infection of Protaetia brevitarsis seluensis larvae using several effective microorganisms

  • Kwak, Kyu-Won;Kwon, Soon Woo;Nam, Sung-Hee;Park, Kwan-Ho;Kim, Eun-Sun;Lee, Hee-Sam;Choi, Ji-Young;Han, Myung-Sae
    • International Journal of Industrial Entomology and Biomaterials
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    • v.36 no.1
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    • pp.1-9
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    • 2018
  • The purpose of this study was to determine the best method for minimizing the occurrence of Metarhizium anisopliae infection of Protaetia brevitarsis seluensis during mass breeding on agricultural farms. There is a high demand for the use of P. b. seluensis larvae in animal feed and as food for humans. However, mass breeding results in the entomopathogenic fungal (usually M. anisopliae) infection of P. b. seluensis. A mixture of microorganisms (Bacillus subtilis, Lactobacillus plantarum, and Saccharomyces cerevisiae) delayed fungal infection by M. anisopliae, which infected fewer P. b. seluensis when the microorganism mixture was added to sawdust as feed for P. b. seluensis. When sawdust with the effective microorganisms (EM) was given to P. b. seluensis for 30 d, their mortality rate was approximately 35 % less than that of the control group, which was fed sawdust without the EM. In addition, the growth of M. anisopliae on agar media spread with each bacterium as inhibited by up to 80 % more than those spread with 4 % sodium hypochlorite, which is a harmless fungal inhibitor generally used in agricultural farms for disinfection.

Development of Model Estimating Fertility Rate for Korea (출산율 예측 모형 개발)

  • Lee, Sam-Sik;Choi, Hyo-Jin
    • Korea journal of population studies
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    • v.35 no.1
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    • pp.77-99
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    • 2012
  • This study aimed at developing a model for estimating fertility rates for Korea under some conditions. The model is expected to provide the basic information for establishing and evaluating the polices in prompt and adequate response to low fertility and population ageing. The model was established on the basis of experiences by some OECD countries in Europe, having experienced the fertility increase trend and being economically well-developed, because Korea has never experienced the steady increase in fertility rate since 1960. This study collected about 20 years' time series data for each of selected countries and applied to the regression model, which is called a 'panel analysis' to take into considerations both cross-sectional and longitudinal aspects of fertility change simultaneously. Simulation of the model for Korea and some panel countries showed a very small difference, less than 0.1, between the estimated rate and the observed rate for each year during 2006~2010. Thus, the model, as established in this study, is evaluated as accurate or well-fitted to a considerable extent.

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The effect of Dynamic and Static PNF stretching on lower back flexibility (동적, 정적, PNF 스트레칭이 요부 유연성에 미치는 효과)

  • Choi, Won-Jye;Cho, Nam-Jung;Kang, Hee-Kwon;Kang, Soo-Kyung;Kim, Min-Jung;Kim, Bung-Oh;Heo, Soo-Yong;Yoo, Byung-Kook;Lee, Sam-Cheol;Son, Kyung-Hyun
    • PNF and Movement
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    • v.7 no.2
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    • pp.11-20
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    • 2009
  • Purpose : The purpose of this study is to find out which method is efficient to enhance the flexibility of lower back between PNF(Proprioceptive Neuromuscular Facilitation), static and dynamic stretching. Methods : Subjects were 30 young people between the ages 17 and 19. They were randomly divided into three groups; static group(n=10) performed a static stretching, dynamic group(n=10) performed a dynamic stretching, and PNF group(n=10) performed a PNF stretching. Intervention was provided 5 days per week for 4 weeks. For each case, Trunk flexion forward, trunk flexion backward, trunk left lateral bending, trunk right lateral bending, trunk flexion forward a measuring instrument and tapeline were performed to measured the flexibility of lower back at different times(before starting the exercise, after 4 weeks). Results : The results of the study reveal that the lower back flexibility was a statistically significant difference in all groups(p<.05). There was statistically significant difference between PNF group and static group, PNF group and dynamic group. Conclusion : The finding indicated that PNF may be preferred technique for improving flexibility, and that flexibility training results in an increased consistency of flexibility scores.

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Effect on fruit growth and quality as artificial pollination time of Dragon fruit(Hylocereus undatus) in heating plastic house cultivation (열대과수 용과 가온하우스 재배의 인공수분 시간이 과실비대에 미치는 영향)

  • Park, Jae-ok;Lee, Somi;Cho, Kyung-chul;Kwon, Hye-young;Kim, Byung-sam;Hwang, In-taek
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2018.10a
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    • pp.79-79
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    • 2018
  • 용과(Hylocereus undatus)는 멕시코 및 과태말라가 원산지이며 아열대 및 열대지역에서 재배되고 있으며 아시아는 베트남 타이완 중국 일본 등 국가에서 재배하고 있다. 가지에 열매가 있는 모습이 용이 여의주를 물고 있는 형과 닮았다하여 붙여진 이름이다. 지구온난화로 인한 기온상승으로 전남지역 재배에 적합한 대체 열대 및 아열대과수 개발이 필요하다. 용과는 열대과수 중 비교적 낮은 온도($10^{\circ}C$이상) 조건에서 재배 가능하여 남부지역에서 시설내 가온재배하고 있다. 국내 시설재배 중 문제점은 용과는 밤에 꽃을 피기 때문에 밤에 활동하는 매개곤충이 없어서 인공수분으로 수분문제를 해결해야한다. 인공수분의 한계시간과 수분할 경우 과실비대에 미치는 영향을 알아보고자 인공수분 시간을 개화 1시간 이내, 4~5시, 11~12시 및 무 처리(방임)로 처리하였다. 개화시기는 1차 7월 13일, 2차 8월 8일, 3차 8월 26일로 3회에 걸쳐 개화가 이루어졌으며 이시기의 화분량는 4~5시가 0.68g, 11~12시 0.47g로 1시간 이내 0.16g보다 많았고 과실비대에서 1과중은 개화11~12시가 421g, 4~5시 400g, 1시간 이내가 377g으로 처리 간에 차이는 없었지만 무처리는 185g으로 처리구와 192~236g의 유의차가 있었다. 용과의 인공수분한계시간은 다음날 아침 7시(개화 11~12시)까지 인공수분 할 경우 정상적인 착과가 이루어졌으며 과실비대를 위해서 인공수분은 필수적으로 실시해야 한다.

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Effect on fruit coloration for double harvesting by flowering period of passionfruit(Passiflora edulis) in non-heating plastic house cultivation (패션프루트 무가온하우스 재배의 후기 수분시기가 착색에 미치는 영향)

  • Park, Jae-ok;Lee, Somi;Cho, Kyung-chul;Kim, Byung-sam;Hwang, In-taek
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2018.10a
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    • pp.78-78
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    • 2018
  • 패션프루트(Passiflora edulis)는 브라질이 원산지이며 아열대와 열대지역에서 재배되고 있는 다년생 상록덩굴식물로 시계꽃과(Passifloraceae) 시계꽃속(Passiflora)에 속한다. 전 세계적으로 500여종이 분포하고 그 중 10여종은 식용, 나머지는 관상용으로 이용되고 있다. 지구온난화로 인한 기온상승으로 전남지역 재배에 적합한 대체 아열대과수 개발이 필요하다. 패션프루트는 겨울 최저온도가 $3^{\circ}C$이상 조건에서 재배 가능하여 남부 지역는 무가온 하우스재배를 하고 있지만 시설내 6월 하순부터 8월 중순까지 $35^{\circ}C$ 이상 고온이 지속되어 과실을 볼 수 없다. 8월 하순부터 피는 꽃은 인공수분으로 착과되어 11월부터 수확이 가능하지만 이후에 낮은 온도($10^{\circ}C$이하)로 착색이 진행되지 않아서 동해피해로 수확을 포기해야 한다. 어느 시기까지 인공수분을 해야 당년에 안정적인 수확이 가능한지를 알아보고자 패션프루트의 후기(2차) 개화시기 중에서 8월 30일, 9월 10일, 9월 20일, 9월 30일에 인공수분 하여 11월 하순에 착색이 정상적으로 진행되는 처리를 조사한 결과 8월 30일 처리구만 11월 21일 착색이 되었다. 미착색과에서 내용물이 진노랑색으로 진행되면 후숙하여 상품과로 판매가 가능하기 때문에 진노랑색에 도달한 시기를 조사하였다. 조사에서 9월 10일까지 인공수분 처리한 과실은 후숙하여 상품과로 판매가 가능하였다.

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Toxic Algal Bloom Caused by Dinoflagellate Alexandrium tamarense in Chindong Bay, Korea

  • Yoo Jong Su;Fukuyo Yasuwo;Cheun Byeungsoo;Lee Sam Geun;Kim Hak Gyoon
    • Fisheries and Aquatic Sciences
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    • v.3 no.1
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    • pp.26-32
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    • 2000
  • Monospecific red tide by a toxic dinoflagellate belonging to the genus Alexandrium occurred at Chindong Bay in the southern coast of Korea and continued from April 6th to 15th in 1997. The ratio of its cell number to total phytoplankton cell number was much higher than $95\%$. This organism was identified as Alexandrium tamarense, although slight morphological differences were found comparing to the original and successive descriptions of the species. We found neither anterior nor posterior attachment pores in these cells of the bloom population. The occurrence of red tide caused by A. tamarense was first reported in Korea. Its plate formula is Po, Pc, 4', 6"c, 8s, 5"' and 2"". Thecal plates are thin with pore-like ornamentation. In those plates, the anterior part of the first apical plate (1') is narrower and its posterior end has sometimes a block-like accessory, but this variation was considered within the range of the morphological variability of this taxon. The cell density during the red tide exhibited a wide range of variation by the depth of water column, ranging from $2\times10^6$ cells$l^{-1}$ to $5\times10^6$ cells·$l^{-1}$. Water temperature varied from 11.8 to $12.3^{\circ}C$. Toxicity of A. tamarense during red tide was measured as $8.8\times10^5$. $MU\;\cdot\;cell^{-1}$ by mouse bioassay.

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Reliable extraction of moving edge segments in the dynamic environment (동적인 입력환경에서 신뢰성이 있는 이동 에지세그먼트 검출)

  • Ahn Ki-Ok;Lee June-Hyung;Chae Ok-Sam
    • Journal of the Institute of Electronics Engineers of Korea SP
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    • v.43 no.5 s.311
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    • pp.45-51
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    • 2006
  • Recently, the IDS(Intrusion Detection System) using a video camera is an important part of the home security systems which start gaining popularity. However, the video intruder detection has not been widely used in the home surveillance systems due to its unreliable performance in the environment with abrupt illumination change. In this paper, we propose an effective moving edge extraction algerian from a sequence image. The proposed algorithm extracts edge segments from current image and eliminates the background edge segments by matching them with reference edge list, which is updated at every frame, to find the moving edge segments. The test results show that it can detect the contour of moving object in the noisy environment with abrupt illumination change.

AN EXPERIMENTAL STUDY ON THE CHANGE OF THE RADIOSENSITIVITY OF SEVERAL TUMOR CELL LINES AND PRIMARY CULTURED GINGIVAL FIBROBLAST (수종의 암세포주와 치은섬유아세포에서 방사선의 양과 분할조사에 따른 세포활성도와 독성의 변화에 관한 연구)

  • Lee Sam-Sun;You Dong-Soo
    • Journal of Korean Academy of Oral and Maxillofacial Radiology
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    • v.27 no.1
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    • pp.107-122
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    • 1997
  • Radiation sensitivity data was generated for two human cancer cell lines(KB, RPMI 2650) and human primary gingival fibroblast was tested three times using a viable cell number counting with a hemocytometer, MTT(3-[4,5-Dimethylthiazol2-yl]-2,5-diphenyl tetrazolium bromide) assay, and LDH(Lactate dehydrogenase) assay. Single irradiation of 2, 4, 6, 10, 15, 20Gy were applied to the tumor cell lines and the primary cultured gingival fibroblast The two fractions of 4Gy and 10Gy were seperated with a 4 hour time interval. The irradiation was done with 241.5cGy/min dose rate using /sup 137/Cs MK cell irradiator at room temperature. The obtained results were as followed : 1. There was significantly different viable cell numbers as the amount of radiation dose on the tested cells were cell number counted with a hemocytometer. In fractions, there were more viable cells remaining. 2. Phase-contrast microscopically, radiation-induced morphologic changes were pronounced on the tumor cells, however, almost no differences on the gingival fibroblast. 3. There was significantly different absorbance at 2Gy on RPMI 2600, 4Gy on KB and GF in MTT assay. In fractions, the absorbance was significantly higher on KB. 4. The level of extracellular LDH activity in the experimental group was significantly higher in the 2-4Gy than the control group. 5. The total level of extracellular and intracellular LDH activity was decreased as increased amounts of radiation dose was applied.

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A CASE REPORT OF ANEURYSMAL BONE CYST OF THE MANDIBULAR CONDYLE (하악과두에 발생한 동맥류성골낭)

  • Ko Jae-Hee;Lee Sam-Sun;Choi Soon-Chul;Park Tae-Won;You Dong-Soo
    • Journal of Korean Academy of Oral and Maxillofacial Radiology
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    • v.27 no.1
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    • pp.231-241
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    • 1997
  • The aneurysmal bone cyst is a nonmalignant reactive bone lesion. Developing rarely in the craniofacial region, and more commonly affecting the long bones and the spine, the lesion has variable etiopathogenic characteristics. The authors diagnosed a 33-year-old female as aneurysmal bone cyst after undergoing clinical, radiological and histological examinations. The characteristics were as followed: 1. The patient complained of pain and swelling of the right preauricular area. 2. The conventional radiograms showed a relatively well defined radiolucent lesion with partially scalloping margin. The cortical bone of the right condyle was thinned and expanded by the lesion. 3. Bone scintigraphy with ~c demonstrated ring-like or doughnut-pattern accumulation of radioactivity. 4. On Tl-weighted imaging of MRI, the lesion on the right condyle had middle signal intensity. T2-weighted MRI demonstrated multiple high signal intensities seperated by septa which had low signal intensity. Finger in balloon appearance was seen. 5. Histologically, the lesion was composed of large sinusoidal blood spaces lined by fibroblasts and histiocytes. Its fibrous stroma consisted of fibroblstic element, multinucleated giant cells, extravasated erythrocytes and focal hemosiderin pigmentation. New bone formation was also observed around larger sinusoidal spaces.

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THE EXPERIMENTAL STUDY OF THE BONE REGENERATION ON ${\beta}$-TCP IN RABBIT CRANIAL BONE (가토 두개골에서 ${\beta}$-TCP와 자가골 이식에 관한 실험적 연구)

  • Lee, Sung-Hoon;Song, Seung-Il;Han, Ji-Young;Hwang, Kyung-Gyun;Paik, Sung-Sam;Shim, Kwang-Sup
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.30 no.4
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    • pp.282-291
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    • 2004
  • Pure-phase beta-tricalcium phosphate(${\beta}$-TCP) proved to be a bone regeneration material, providing the patient with vital bone at the defect site in a reasonable time, making a second surgical procedure for bone harvesting unnecessary. This study compares bone healing and BMP 2/4 expression in cranial defects in rabbits grafted with autogenous bone and ${\beta}$-TCP. Thirty New Zealand White rabbits was divided into 3 group of 10 animals each. Bilateral calvarial defects were made in the parietal bones of each animal. ${\beta}$-TCP placed in one defect and the other defects was filled with autogenous bone. The animal were sacrificed at 4, 8 and 12 weeks. Immunohistochemical analysis was used to investigate the expression of BMP 2/4. 1. The new bone formation around autogenous bone from 4 weeks and ${\beta}$-TCP from 8 weeks. 2. In autogenous bone graft, BMP 2/4 expression was decreased from 4 to 12 weeks. 3. In ${\beta}$-TCP graft, BMP 4 expression was increased from 8 to 12 weeks. But, BMP 2 was observed from 12 weeks. This study showed that bone healing, regeneration and, BMP 2/4 expression are delayed in grafted ${\beta}$-TCP than autogenous bone.