• Title/Summary/Keyword: Lee Sam

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Phytochemical Studies on Lonicera Caulis (1) - Sterols and Triterpenoids (인동의 성분연구 (1) - Sterol 및 Triterpenoid 화합물)

  • Kim, Ju-Sun;Yean, Min-Hye;Lee, So-Young;Lee, Je-Hyun;Kang, Sam-Sik
    • Korean Journal of Pharmacognosy
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    • v.40 no.4
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    • pp.319-325
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    • 2009
  • Twelve compounds were isolated from the 70% ethanol extract of Lonicera Caulis (Caprifoliaceae) and their structures were identified as six triterpenoids [(24S)-cycloart-25-en-$3{\beta}$,24-diol (1), pomolic acid (7), ursolic acid (8), euscaphic acid (9), hederagenin (10), and 23-hydroxytormentic acid (12)] and six sterols [obtusifoliol (2), gramisterol (3), citrostadienol (4), ${\beta}$-sitosterol (5), ergosterol peroxide (6) and ${\beta}$-sitosterol glucoside (11)]. The chemical structures of these compounds were identified on the basis of spectroscopic methods and comparison with literature values. All the compounds were isolated from this plant parts for the first time.

Isolation of Two cDNAs Encoding a Putative Peptidohlycan Recognition Protein Gene from the Domestic Silkworm, Bombyx mori

  • Kim, Sang-Hyun;Lee, Heui-Sam;Kim, Jin-Won;Lee, Young-Sin;Kim, Iksoo
    • International Journal of Industrial Entomology and Biomaterials
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    • v.4 no.1
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    • pp.31-36
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    • 2002
  • Peptidohlycan recognition protein (PGRP) is one of the pattern recognition proteins in innate immunity of insect. We isolated differentially expressed two cDNAa, BTL-LPI and BTL-LP2, in the fat body of Bombyx mori larvae injected with bacteria by subtractive hybridization method. These two clones showed amino acid sequence divergence of 30.4%. In the comparison with other insect PGRP genes, BTL-LP2 showed 48.8% and 45.2% of sequence homology to the known PGRP genes of Bombyx mori and Tricoplusia ni, respectively, and BTL-LP2 was 31.8% and 30.9% , respectively. Phylogenetic analysis showed relatively close relationship of the BTL-LP2 to the known insect PGRP, unlike BTL-LPI, which was equidistant both to insect and mammals, suggesting a divergent relationships of the two newly cloned B. mori PGRP genes. Northern blot analyses confirmed an induction of the expression of BTL-LP2 by the bacterial infection in the Int body of B. mori, suggesting the involvement of the gene in the insect immunity.

Estimation of Precise Relative Position using INS/Vision Sensor Integrated System (INS/비전 센서 통합 시스템을 이용한 정밀 상대 위치 추정)

  • Chun, Se-Bum;Won, Dae-Hee;Kang, Tae-Sam;Sung, Sang-Kyung;Lee, Eun-Sung;Cho, Jin-Soo;Lee, Young-Jae
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.36 no.9
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    • pp.891-897
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    • 2008
  • GPS can provide precise relative navigation information. But it needs a reference station in a close range and is effected by satellite observation environment. In this paper, we propose INS and Vision sensor integrated system with a known landmark geometry. This system is supposed to overcome problems of GPS only system. Using the proposed method, a relative navigation is available without a GPS reference station. The only need for the proposed system is a landmark image which is drawn on the ground. We conduct simple simulation to check the performance of this method. As a result, we confirm that it can improve the relative navigation information.

Fine Needle Aspiration Cytology of Pilomatrixoma - Two Cases Report - (모기질세포종의 세침흡인 세포학적 소견 - 2예 보고 -)

  • Paik, Seung-Sam;Kim, Dong-Hoon;Lee, How-Jin;Jang, Se-Jin;Park, Moon-Hyang;Lee, Jung-Dal
    • The Korean Journal of Cytopathology
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    • v.8 no.2
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    • pp.155-159
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    • 1997
  • Pilomatrixoma is a benign skin appendage tumor of hair matrix cell origin. The cytological appearance of this tumor may cause problems when attempting to establish a differential diagnosis with other neoplasms. Herein we report the aspiration cytology findings of two cases of pilomatrixoma. One case was diagnosed as atypical cell clusters initially and the other was diagnosed as pilomatrixoma. On cytologic examination, both cases showed clusters of tightly arranged basaloid cells with an uniform appearance, scanty cytoplasm and round hyperchromatic nuclei. There were histiocytes and multinucleated foreign body giant cells merged with the amorphous opaque orangeophilic materials of "ghost cells". The differential diagnosis with other neoplasms is discussed.

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Membrane Perturbation Induced by Papiliocin Peptide, Derived from Papilio xuthus, in Candida albicans

  • Lee, June-Young;Hwang, Jae-Sam;Hwang, Bo-Mi;Kim, Jin-Kyoung;Kim, Seong-Ryul;Kim, Yang-Mee;Lee, Dong-Gun
    • Journal of Microbiology and Biotechnology
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    • v.20 no.8
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    • pp.1185-1188
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    • 2010
  • Previously, papiliocin was isolated from the swallowtail butterfly Papilio xuthus and its antimicrobial activity was suggested. In this study, the antifungal mechanism of papiliocin against Candida albicans was investigated. Confocal laser scanning microscopy (CLSM) and 1,6-diphenyl-1,3,5-hexatriene (DPH) fluorescence analysis indicated that papiliocin disturbed the fungal plasma membrane. Moreover, the assessment of the release of FITC-dextran (FD) from liposomes further demonstrated that the antifungal mechanism of papiliocin could have originated from the pore-forming action and that the radius of the pores was presumed to be anywhere from 2.3 to 3.3 nm.

A Study on the Characteristic of the $^6Li$ Neutron Spectrometer ($^6Li$ 중성자분광계 특성 연구)

  • Choe, Seong-Ho;Kang, Sam-Woo;Lee, Kwang-Pill;Lee, Kyung-Ju;Hwang, Sun-Tae
    • Analytical Science and Technology
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    • v.5 no.1
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    • pp.57-61
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    • 1992
  • For the neutron spectrum measurement, $^6Li$ neutron spectrometer system is installed. The characteristic of the $^6Li$ detector are investigated using a $^{137}Cs$ and $^{207}Bi$ point source, and the neutron capture peaks and the pulse height spectrum using an $^{214}Am-Be$ neutron source are measured. Furthermore, the pulse height spectrum for the irradiation time variation from the (214)^Am-Be neutron source, and for the distance variation between detector and source, and the threshold variation of discriminator are measured.

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Estimation of optimal phosphorus and calcium levels on the growth and cast production of earthworm(Eisenia foetida) (지렁이의 생육과 분립생산을 위한 적정 인과 칼슘 수준의 추정)

  • Lee, Ju-Sam;Lee, Pil-Won
    • Journal of the Korea Organic Resources Recycling Association
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    • v.10 no.4
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    • pp.96-102
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    • 2002
  • The purpose of this study was estimate the optimal phosphorus and calcium levels of Korean native cow manure on the growth and cast production of earthworm(Eisenia foetida). Phosphorus and calcium levels supplemented to cow manure were 0%(without earthworm), 2% and 4%, and 0.01%, 0.02%, 0.04% and 0.06% of the cow manure by weight, respectively. The maximum fresh weight and increasing rate of earthworm were obtained with 2% phosphorus level, but there was not significant differences between calcium levels. The ratios of available phosphorus to calcium contents of cast were 1.04-1.20:1 with 2%, and 1.28-1.37:1 with 4% phosphorus levels. Conversion rate of organic matter to earthworm tissue were 0.35-0.41% with 2%, and 0.25-0.40% with 4% phosphorus levels.

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Cloning, Sequencing and Characterization of Acyltransferase Gene Involved in Exopolysaccharide Biosynthesis of Zoogloea ramigera 115SLR

  • Lee Sam-Pin;Troyano Esperanza;Lee Jin-Ho;Kim Hyun-Soo;Sinskey Anthony John
    • Journal of Microbiology and Biotechnology
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    • v.16 no.7
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    • pp.1163-1168
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    • 2006
  • The recombinant plasmid pLEX2FP complements the mutation in Zoogloea ramigera 115MM1, and the complemented mutant produces an exopolysaccharide that shows higher affinity for the calcofluor dye than the exopolysaccharide from Z. ramigera 115SLR, resulting in higher fluorescence intensity under UV light. A compositional and structural analysis of the exopolysaccharide from Z. ramigera 115MM1 showed that the different fluorescent properties were due to a lower content of acetyl groups when compared with Z. ramigera 115SLR exopolysaccharide. These results were in agreement with a sequence analysis of the gene carried in the plasmid pLEX2FP, which appeared to encode an O-acyltransferase highly homologous to the 3-O-acyltransferase of Streptomyces mycarofaciens. The gene encoding the acyltransferase from Z. ramigera 115SLR was expressed as a GST-fusion protein with 70,000 daltons in E. coli.

Effect of Aquatic Exercise Program on Lower Extremity Muscle Strength, Balance and Gait Activity in Elderly Women (수중운동 프로그램 참여가 여성노인의 하지근력과 균형 및 보행능력에 미치는 영향)

  • Oh, Sang Boo;Lee, Hyun Chul;Lee, Sam Cheol
    • Journal of Korean Physical Therapy Science
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    • v.20 no.1
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    • pp.89-98
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    • 2013
  • Purpose : The purpose of this study was to investigate the effect of the aquatic exercise program on lower muscle strength, balance and gait activity on 12 weeks aquatic exercise program in the elderly women. Methods : The Subjects in this study were 20 aged 70s who were long-term residents admitted to elderly nursing home of S, Gyeongsangnam province. They performed aquatic exercise programs 60-minute sessions, 3 times a week for 12 weeks. The physical evaluation was used for measuring lower muscle strength, balance activity was used for measuring balance testing, and gait activity was used for measuring gait velocity and maximal step length evaluation. All data were analyzed by paired t-Test by SPSS(version18.0). P-values of <.05 were considered significant. Results : Lower extremity muscle strength, static dynamic balance and gait activity were significantly increased both in the exercise group and between groups. Conclusion : This study suggests that aquatic exercise program has an effect on lower extremity muscle strength, balance and gait activity of the elderly women. Therefore, it is considered as a safe and helpful self-support activity for the elderly people.

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A Design of Navigation System Using Stratospheric Airships in South Korea

  • Lee, Eun-Sung;Chun, Se-Bum;Lee, Young-Jae;Hur, Jung;Kang, Tae-Sam
    • International Journal of Aeronautical and Space Sciences
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    • v.7 no.2
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    • pp.56-69
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    • 2006
  • For a relatively small country like Korea, a radionavigation system using airships can be considered, which is to provide the navigation service utilizing the stratospheric airships that are deployed in the stratosphere at the altitude of around 20-23km, and which is an independent or a back-up radionavigation system other than current GPS or GLONASS. In this paper, a feasibility study on the constellation of stratospheric airships for the navigation system has been performed. A measure of a geometrical condition between a receiver and navigation transmitters. called the DOP (Dilution of Precision), determines the resulting positioning error of the navigation system, if the error of range measurement is predictable. Therefore, with assumption that the range measurement error of the stratospheric airship navigation system is quite similar to GPS. the several DOP values have been used to evaluate the performance of the navigation system with comparing with the DOP values of GPS as the reference values. To provide the position information of the navigation transmitters to users, a receiver cluster system fixed on the ground, called an IGPS (inverted GPS), is proposed, and the error is also evaluated using the DOP values. Five areas around five major cities in South Korea have been selected, and then by numerical simulations the DOP values are compared those of GPS to assess the performance of the proposed navigation system using stratospheric airships. The possible frequency bands have been proposed. and then link budget of the navigation transmitter has been analyzed for the proposed navigation system.