International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
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Isolation of Two cDNAs Encoding a Putative Peptidohlycan Recognition Protein Gene from the Domestic Silkworm, Bombyx mori

  • Kim, Sang-Hyun (Department of Sericulture and Entomology, National Institute of Agricultural Science and Technology, Rural Development Administration) ;
  • Lee, Heui-Sam (Department of Sericulture and Entomology, National Institute of Agricultural Science and Technology, Rural Development Administration) ;
  • Kim, Jin-Won (Department of Sericulture and Entomology, National Institute of Agricultural Science and Technology, Rural Development Administration) ;
  • Lee, Young-Sin (Department of Sericulture and Entomology, National Institute of Agricultural Science and Technology, Rural Development Administration) ;
  • Kim, Iksoo (Department of Sericulture and Entomology, National Institute of Agricultural Science and Technology, Rural Development Administration)
  • Published : 2002.03.01
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Abstract

Peptidohlycan recognition protein (PGRP) is one of the pattern recognition proteins in innate immunity of insect. We isolated differentially expressed two cDNAa, BTL-LPI and BTL-LP2, in the fat body of Bombyx mori larvae injected with bacteria by subtractive hybridization method. These two clones showed amino acid sequence divergence of 30.4%. In the comparison with other insect PGRP genes, BTL-LP2 showed 48.8% and 45.2% of sequence homology to the known PGRP genes of Bombyx mori and Tricoplusia ni, respectively, and BTL-LP2 was 31.8% and 30.9% , respectively. Phylogenetic analysis showed relatively close relationship of the BTL-LP2 to the known insect PGRP, unlike BTL-LPI, which was equidistant both to insect and mammals, suggesting a divergent relationships of the two newly cloned B. mori PGRP genes. Northern blot analyses confirmed an induction of the expression of BTL-LP2 by the bacterial infection in the Int body of B. mori, suggesting the involvement of the gene in the insect immunity.

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References

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