• Title/Summary/Keyword: Lee Gyu Jun

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Low-cost Assessment of Canopy Light Interception and Leaf Area in Soybean Canopy Cover using RGB Color Images (RGB 컬러 이미지를 이용한 콩의 군락 피복과 엽면적에 대한 저비용 평가)

  • Lee, Yun-Ho;Sang, Wan-Gyu;Baek, Jae-Kyeong;Kim, Jun-Hwan;Cho, Jung-Il;Seo, Myung-Chul
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.22 no.1
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    • pp.13-19
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    • 2020
  • This study compared RGB color images with canopy light interception (LI) and leaf area index (LAI) measurements for low cost and low labor. LAI and LI were measured from vertical gap fraction derived from top of digital image in soybean canopy cover (cv Daewonkong, Deapongkong and Pungsannamulkong). RGB color images, LAI, and LI were collected from V4.5 stage to R5stage. Image segmentation was based on excess green minus excess red index (ExG-ExR). There was a linear relationship between LAI measured with LI (r2=0.84). There was alinear relation ship between LI measured with canopy cover on image (CCI) (r2=0.94). There was a significant positive relationship(r2=0.74) between LAI and CCI at all grow ingseason. Therefore, it is expected that in the future, the RGB color image could be able to easily measure the LAI and the LI at low cost and low labor.

Study of six different commercial Koran-native chicken crossbreeds from hatch to twelve weeks of age

  • Shin, Taeg Kyun;Wickramasuriya, Samiru Sudharaka;Kim, Eun Joo;Cho, Hyun Min;Hong, Jun Seon;Lee, Hyun Gyu;Heo, Jung Min;Yi, Young-Joo
    • Korean Journal of Agricultural Science
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    • v.44 no.4
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    • pp.531-540
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    • 2017
  • A study was conducted to compare growth performance of six female commercial Korean native chicken (KNC) crossbreeds from hatching to twelve weeks of age. Three hundred and twelve, 1-day-old female commercial KNC were used within 1 paternal line and 6 maternal lines. The chickens were allocated to 24 battery cages to give 4 replicates per strain with 13 chickens per cage. The chickens were reared under continuous lighting (24 h) and water was available at all times. Ad-libitum feeding was practiced throughout the experimental period. Among the six different strains, 2A had the greatest bodyweight (BW) at 42 days after hatching (p < 0.05). No BW difference between six crossbreed strains (p > 0.05) was found thereafter. Crossbreed 1A had the higher average daily gain (ADG) than crossbreed 2A and 3A chickens (p < 0.05), whereas crossbreed 4A, 5A, and 6A had similar ADGs to that of crossbreed 1A (p > 0.05) at 84 days after hatching. Furthermore, crossbreed 4A had a great average daily feed intake (ADFI) from hatching to 84 days (p < 0.05). Nonetheless, there was no difference in the feed conversion ratio (FCR) and uniformity between six crossbreed strains for the experimental period (p > 0.05). Despite that 1A, 4A, and 6A had the higher viability (p < 0.05) than crossbreed 2A and 5A, they had a similar viability than crossbreed 3A (p > 0.05). With this in mind, crossbreed 2A had greater BW, ADG, and FCR than other chicken crossbreeds from hatching to 84 days, although they had a lower viability than others.

A Study of Shrinkage Characteristics of Low Shrinkage Normal Strength Concrete With Boundary Restraint Condition (4변 구속조건을 갖는 초저수축 일반강도 콘크리트의 수축특성 연구)

  • Jeong, Jun-Young;Min, Kyung-Hwan;Lee, Dong-Gyu;Choi, Hong-sik
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.17 no.6
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    • pp.693-699
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    • 2016
  • In this study, the replacement effects of cementitious materials (fly ash, blast furnace slag, and blended mixtures) were assessed for normal strength concrete with very low shrinkage properties under $350{\mu}{\varepsilon}$ strain using a powder type shrinkage reducing agent. In addition, through mock-up tests of actual size walls restrained with four sides, the shrinkage characteristics using the power type shrinkage reducing agent were measured and the crack reducing ability was assessed. The slump and air contents were measured as the properties of fresh concrete, and the length changes of the prismatic specimens, $100{\times}100{\times}400mm$ in size, were measured for the shrinkage characteristics. To reduce the shrinkage of concrete, the maximum replacing ratio of the fly ash is effective to 20 percent; however, the use of blast furnace slag and ternary mixtures did not reduce the shrinkage.

Inhibitory Effect of Aqueous Extract from Lonicera japonica Flower on LPS-induced Inflammatory Mediators in RAW 264.7 Macrophages. (금은화 수용성 추출물의 LPS 유도 염증매개물 억제 효과)

  • Yun, Young-Gab;Kim, Gyu-Min;Lee, Sung-Jun;Ryu, Seong-Hun;Jang, Seon-Il
    • The Korea Journal of Herbology
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    • v.22 no.3
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    • pp.117-125
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    • 2007
  • Objective : Lonicera japonica (Caprifoliaceae) has long been used for treatment of infectious diseases in oriental countries. The aim of this study was to investigative the effect by which the aqueous extract from flower of L. japonica (LJFAE) inhibited the lipopolysaccharide (LPS)-induced inflammatory mediators in murine macrophages, RAW 264.7 cells Methods : The dried flowers of L. japonica were extracted with distilled water at $100^{\circ}C$ for 7 h. The extract was filtered through 0.45 ${\mu}m$ filter, freeze-dried. The dried extract was dissolved in Hank's balanced salt solution (HBSS) and filtered through 0.22 ${\mu}m$ filter before use. Accumulated nitrite, an oxidative product of nitric oxide (NO), was measured in the culture medium by the Griess reaction. The levels of prostaglandin E2 (PGE2), tumor necrosis factor-$\alpha$ (TNF-$\alpha$), interleukin-1$\beta$ (IL-1$\beta$), and IL-6 production, inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression were measured by enzyme-linked immunosorbent assay and Western blot analysis. Results: LJFAE (10-400 ${\mu}g$/ml) per se had no cytotoxic effect in unstimulated macrophages, but LJFAE concentration-dependently reduced NO, PGE2, TNF-, IL-l, and IL-6 production and COX-2 activity caused by stimulation of LPS. The levels of iNOS and COX-2 protein expressions were markedly suppressed by the treatment with LJFAE in a concentration dependent manner. Conclusions : These results suggest that LJFAE suppress the NO and PGE2production in macrophages by inhibiting iNOS and COX-2 expression and these properties may contribute to the anti-inflammatory activity of Lonicera japonica.

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Effects of Dietary Arachidonic Acid (20:4n-6) Levels on Growth Performance and Fatty Acid Composition of Juvenile Eel, Anguilla japonica

  • Bae, Jun-Young;Kim, Dae-Jung;Yoo, Kwang-Yeol;Kim, Sun-Gyu;Lee, Jeong-Yeol;Bai, Sungchul C.
    • Asian-Australasian Journal of Animal Sciences
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    • v.23 no.4
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    • pp.508-514
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    • 2010
  • This study was conducted to evaluate the effects of dietary arachidonic acid (AA, 20:4n-6) levels on growth performance and body composition in juvenile eel, Anguilla japonica. Six semi-purified experimental diets were formulated to be isonitrogenous and iso-caloric containing 55.0% crude protein and 15% crude lipid (18.3 kJ of available energy $g^{-1}$). Six different levels of AA were added to the basal diet, with 0, 0.2, 0.4, 0.6, 0.8 or 1.2% on a dry matter (DM) basis, respectively ($AA_{0.07},\;AA_{0.22},\;AA_{0.43},\;AA_{0.57},\;AA_{0.78}\;or\;AA_{1.23}$). After a conditioning period, fish initially averaging 27${\pm}$0.5 g (mean${\pm}$SD) were randomly distributed into each aquarium as triplicate groups of 20 fish each. One of six experimental diets was fed on a DM basis to fish in three randomly selected aquaria at a rate of 2-3% of total body weight twice a day. At the end of the 12-week feeding trial, weight gain (WG) and feed efficiency (FE) of fish fed $AA_{0.78}$ and $AA_{1.23}$ diets were significantly higher than of fish fed $AA_{0.07},\;AA_{0.22},\;AA_{0.43}$ diets (p<0.05). Specific growth rate (SGR) of fish fed the $AA_{0.78}$ diet was significantly higher than of fish fed $AA_{0.07},\;AA_{0.22},\;AA_{0.43}$ diets (p<0.05). However, there were no significant differences in WG, SGR and FE among fish fed $AA_{0.57},\;AA_{0.78}\;or\;AA_{1.23}$ diets (p>0.05). Whole body AA deposition of fish fed the $AA_{1.23}$ diet was significantly higher than for the other diets (p<0.05). Broken-line model analysis on the basis of WG and SGR indicated that the dietary AA requirement could be greater than 0.69% but less than 0.71% of the diet in juvenile eel. The growth-promoting activity of AA observed in the present study provides strong support for the contention that dietary AA is essential for juvenile eel.

Effects of the Extracts from Gyrophora esculenta and Coriolus versicolor judae Mycelia on the Growth of Intestinal Bacteria (석이버섯과 운지버섯 균사체 추출물이 장내 세균의 생육에 미치는 영향)

  • Park, Kyoung-Ran;Lee, Woon-Jong;Cho, Min-Gyu;Park, Eui-Seok;Jeong, Jun-Young;Kwon, Oh-Sung;Yoon, Hyang-Sik;Kim, Kwang-Yup
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.39 no.6
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    • pp.820-825
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    • 2010
  • The functional food components from various Basidiomycota were investigated to improve human intestinal microflora, especially associated with obesity. EtOH extract from Gyrophora esculenta fruit body and Coriolus versicolor judae mycelia showed antimicrobial activities on Eubacterium limosum, Clostridium perfrigens, Clostridium paraputrificum, Clostridium difficile and Clostridium ramosum, and on Bacteroides fragilis, respectively. Although the 80% EtOH extract from G. esculenta fruit body and hot-water extract from C. versicolor judae mycelia did not reduce weight of the rats in the high fat diet, these extracts showed stability at high temperatures and at wide pH ranges. In the rat group of feeding 80% EtOH extract from G. esculanta fruit body, Bifidobacterium spp. were increased and Clostridium spp. and Eubacterium spp. were decreased compared to the high fat feeding group. Also sensory evaluation was carried out for the development of prototype drink product. These results demonstrated the possibilities of C. versicolor judae and G. esculenta as a functional food components to control intestinal microbial flora.

Reconstruction of the Thumb, the Second and Third Finger in Patient with Amputation of Right Five Fingers (우측 수부의 모든 수지 절단 환자에서 무지와 제 2, 3 수지 재건술)

  • Lee, Jun-Mo;Kim, Gyu-Hyung
    • Archives of Reconstructive Microsurgery
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    • v.10 no.2
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    • pp.143-148
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    • 2001
  • The hand with amputation of all fingers is useless for activities of daily living and traumatic amputation of some of the fingers can result in the diminished ability to perform power grip and precision grip which is vital to maintain normal function of the hand. Precision grip is used to hold an object between the opposable thumb and flexed fingers. In power grip the object is held between the flexed fingers and the palm while the thumb applies the necessary counterpressure to maintain the grip on the object. A 35 year old male lost his right all fingers including thumb at the level of proximal phalanx from the pressure machinary accident. Thumb was reconstructed using wrap around flap and the second and third fingers were reconstructed using the second and third toe transplantation. Seven years after reconstruction, he uses the reconstructed thumb and the second and the third fingers for eating meals, writing down a paper with a pencil and putting on socks.

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Effect of Methanol Extract of Dryopteris Crassirhizoma in Human Oral Cancer Cells (관중 메탄올추출물이 구강암세포주에 미치는 영향)

  • Jang, Boonsil;Oh, Se-Jun;Shin, Ji-Ae;Lee, Hang-Eun;Jeon, Jae-Gyu;Cho, Sung-Dae
    • Journal of Food Hygiene and Safety
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    • v.29 no.3
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    • pp.248-251
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    • 2014
  • Dryopteris crassirhizoma is one of the naturally occurring substance wood ferns and is known for having anti-inflammatory, antiviral and anthelmintic activities. However, there is less report about its anticancer effect in human cancer cell lines. In the present study, the effect of methanol extract of dryopteris crassirhizoma (MEDC) on apoptosis in human oral cancer cell lines (MC3 and HN22 cells) was investigated. MEDC inhibited cell viability and induced apoptosis. MEDC significantly increased Bak and truncated Bid proteins in MC3 cells and elevated only truncated Bid compared to the control while other Bcl-2 family proteins were not altered. MEDC has anticancer activity by inducing apoptotic cell death through the regulation of either Bak or Bid. These findings suggest that its extract possibly may be used for treating oral cancer.

Effect of Gamma Irradiation on Shelf-life Extension and Sensory Characteristics of Dak-galbi (Marinated Diced Chicken) during Accelerated Storage

  • Yoon, Yo-Han;Cho, Won-Jun;Park, Jin-Gyu;Park, Jae-Nam;Song, Beom-Seok;Kim, Jae-Hun;Byun, Myung-Woo;Kim, Cheon-Jei;Sharma, Arun K.;Lee, Ju-Woon
    • Food Science of Animal Resources
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    • v.29 no.5
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    • pp.573-578
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    • 2009
  • This study examined the effect of gamma irradiation on shelf-life extension and sensory characteristics of dak-galbi. Commercial dak-galbi sauce was gamma-irradiated at 0, 5, 10, 15, 20, 25, and 30 kGy. The dak-galbi sauce (200 g) was then added to diced chicken (800 g) for cooking, and the cooked dak-galbi samples in vacuum bags were stored at $35^{\circ}C$ for 5 d. Dak-galbi samples were analyzed on d 0, 1, 2, 3, 4, and 5 for microbial analysis (plate count agar), thiobarbituric acid (TBARS) and volatile basic nitrogen (VBN) measurements, and on d 0 for sensory evaluation. On d 0, total bacterial populations were below detection limit after dak-galbi marinated with the gamma-irradiated (${\geq}15\;kGy$) sauces were cooked, and the samples marinated with higher dose irradiated dak-galbi sauce had lower (p<0.05) bacterial populations during storage. TBARS values of the dak-galbi samples marinated with non-irradiated sauce were not different ($p{\geq}0.05$) with those marinated with irradiated sauces on d 0, and the TBARS values increased (p<0.05) during storage at $35^{\circ}C$, regardless of irradiation dose. In the VBN analysis, there was no difference ($p{\geq}0.05$) in VBN values among irradiation doses on d 0, but VBN values decreased (p<0.05) as irradiation dose increased during storage. Moreover, there were no significant differences ($p{\geq}0.05$) in sensory characteristics among irradiation doses. These results indicate that use of gamma irradiation on dak-galbi sauce may be useful in shelf-life extension without compromising the sensory characteristics of dak-galbi.

Comparison of Virulence Factors, Phylogenetic Groups and Ciprofloxacin Susceptibility of Escherichia coli Isolated from Healthy Students and Patients with Urinary Tract Infections in Korea

  • Park, Min;Park, Soon-Deok;Kim, Sa-Hyun;Woo, Hyun-Jun;Lee, Gyu-Sang;Kim, Hyun-Woo;Yang, Ji-Young;Cho, Eun-Hee;Uh, Young;Kim, Jong-Bae
    • Biomedical Science Letters
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    • v.18 no.2
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    • pp.146-151
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    • 2012
  • Urinary tract infection (UTI) is one of the most common bacterial infections and is predominantly caused by uropathogenic Escherichia coli (UPEC). UPEC strains generally possess several genes encoding virulent factors, which are mostly adhesins, toxins, bacteriocin and siderophores. E. coli is composed of four main phylogenetic group (A, B1, B2, D) and virulent extra-intestinal strains mainly belong to groups B2 and D. Prescription of ciprofloxacin, a kind of fluoroquinolone group antibiotics, is increasing now a days, but resistance to this drug is also increasing. A total of 188 strains of E. coli were collected. Thirteen strains were collected from healthy students in 2011 and 175 strains from patients with urinary tract infection in 2010. Virulence factor genes (papC, fimG/H, sfaD/E, hlyA, cnf1, and usp) were amplified by polymerase chain reaction (PCR) methods for phylogenetic group (A, B1, B2, D) detection. Ciprofloxacin susceptibility test was performed by disk diffusion method. The identified virulence factors (VFs), phylogenetic groups and ciprofloxacin resistance in 13 E. coli strains isolated from healthy students were papC (15.4%), fimG/H (76.9%), sfaD/E (30.8%), hlyA (23.1%), cnf1 (23.1%), usp (7.7%), phylogenetic group A (23%), B1 (8%), B2 (46%), D (23%) and ciprofloxacin resistance (7.7%), while those of in 175 E. coli strains isolated from patients with UTI were papC (41.1%), fimG/H (92.5%), sfaD/E (30.3%), hlyA (10.3%), cnf1 (30.3%), usp (27.4%), phylogenetic group A (9.1%), B1 (5.1%), B2 (60.6%), D (25.1%) and ciprofloxacin resistance (29.7%). In this study, 10 out of 13 E. coli strains (76.9%) from healthy students were found to possess more than one virulence factor associated with adhesion. In addition, one E. coli strain isolated from healthy students who had never been infected with UPEC showed ciprofloxacin resistance. According to these results between the virulence factors and phylogenetic groups it was closely associated, and UPEC strains isolated from patients showed high level of ciprofloxacin resistance.