Biomedical Science Letters (대한의생명과학회지)

The Korean Society for Biomedical Laboratory Sciences (대한의생명과학회)
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Comparison of Virulence Factors, Phylogenetic Groups and Ciprofloxacin Susceptibility of Escherichia coli Isolated from Healthy Students and Patients with Urinary Tract Infections in Korea

  • Park, Min (Department of Biomedical Laboratory Science, College of Health Science, Yonsei University) ;
  • Park, Soon-Deok (Department of Laboratory Medicine, Yonsei University Wonju College of Medicine) ;
  • Kim, Sa-Hyun (Department of Biomedical Laboratory Science, College of Health Science, Yonsei University) ;
  • Woo, Hyun-Jun (Department of Biomedical Laboratory Science, College of Health Science, Yonsei University) ;
  • Lee, Gyu-Sang (Department of Biomedical Laboratory Science, College of Health Science, Yonsei University) ;
  • Kim, Hyun-Woo (Department of Biomedical Laboratory Science, College of Health Science, Yonsei University) ;
  • Yang, Ji-Young (Department of Biomedical Laboratory Science, College of Health Science, Yonsei University) ;
  • Cho, Eun-Hee (Department of Biomedical Laboratory Science, College of Health Science, Yonsei University) ;
  • Uh, Young (Department of Biomedical Laboratory Science, College of Health Science, Yonsei University) ;
  • Kim, Jong-Bae (Department of Biomedical Laboratory Science, College of Health Science, Yonsei University)
  • Received : 2012.03.08
  • Accepted : 2012.06.04
  • Published : 2012.06.30
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Abstract

Urinary tract infection (UTI) is one of the most common bacterial infections and is predominantly caused by uropathogenic Escherichia coli (UPEC). UPEC strains generally possess several genes encoding virulent factors, which are mostly adhesins, toxins, bacteriocin and siderophores. E. coli is composed of four main phylogenetic group (A, B1, B2, D) and virulent extra-intestinal strains mainly belong to groups B2 and D. Prescription of ciprofloxacin, a kind of fluoroquinolone group antibiotics, is increasing now a days, but resistance to this drug is also increasing. A total of 188 strains of E. coli were collected. Thirteen strains were collected from healthy students in 2011 and 175 strains from patients with urinary tract infection in 2010. Virulence factor genes (papC, fimG/H, sfaD/E, hlyA, cnf1, and usp) were amplified by polymerase chain reaction (PCR) methods for phylogenetic group (A, B1, B2, D) detection. Ciprofloxacin susceptibility test was performed by disk diffusion method. The identified virulence factors (VFs), phylogenetic groups and ciprofloxacin resistance in 13 E. coli strains isolated from healthy students were papC (15.4%), fimG/H (76.9%), sfaD/E (30.8%), hlyA (23.1%), cnf1 (23.1%), usp (7.7%), phylogenetic group A (23%), B1 (8%), B2 (46%), D (23%) and ciprofloxacin resistance (7.7%), while those of in 175 E. coli strains isolated from patients with UTI were papC (41.1%), fimG/H (92.5%), sfaD/E (30.3%), hlyA (10.3%), cnf1 (30.3%), usp (27.4%), phylogenetic group A (9.1%), B1 (5.1%), B2 (60.6%), D (25.1%) and ciprofloxacin resistance (29.7%). In this study, 10 out of 13 E. coli strains (76.9%) from healthy students were found to possess more than one virulence factor associated with adhesion. In addition, one E. coli strain isolated from healthy students who had never been infected with UPEC showed ciprofloxacin resistance. According to these results between the virulence factors and phylogenetic groups it was closely associated, and UPEC strains isolated from patients showed high level of ciprofloxacin resistance.

Keywords

References

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