• Mycobiology
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• v.36 no.3
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• pp.183-189
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• 2008

The production of aerial conidia of Lecanicillium lecanii 41185, a highly virulent fungus, by solid-state fermentation was studied for use as a biocontrol agent against aphids. Among several agro-industrial solid media, steamed polished rice was found to produce the highest amount of aerial conidia. The optimal conditions for aerial conidia production were determined to be a 28.5% moisture content in the rice, 25$^{\circ}C$ culture temperature, rice pH of 6.0, 75% ambient relative humidity, 4-dold seeding culture, 0.6% $KNO_3$, and 12 d of culture time. The conidia yield increased from $5.7\times10^9$ conidia/g polished rice to 18.2 $18.2\times10^9$ conidia/g polished rice following application of these optimized conditions.

• Korean journal of applied entomology
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• v.49 no.4
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• pp.417-422
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• 2010

Cultural characteristics Lecanicillium lecani Btab01 and its insecticidal activity against tobacco whitefly (Bemisia tabaci) were investigated. On potato dextrose agar, tryptic soy agar and SDA+Y media, mycelial growth of L. lecani Btab01 was best at $20{\sim}25^{\circ}C$ and suppressed above $28^{\circ}C$. Both solid culture and liquid culture of L. lecani Btab01 showed high insecticidal activity, 93.9 and 98.3% respectively, against nymph of tobacco whitefly, but there is no significant difference. When culture of L. lecani Btab01 was treated at the concentration of $10^5$, $10^6$, $10^7$ and $10^8$ cfu/ml, their insecticidal activity were 5.8%, 33.8%, 77.3% and 98.5% respectively, and $LT_{50}$ values were 16.1 days, 7.3 days, 5.1 days and 3.5 days respectively. When nymphs were treated by the cultures of L. lecani Btab01 and maintained under saturated condition for zero hour, 24 hours and 168 hours, their control activities were 0%, 20.3% and 100% respectively. Spore germination of L. lecani Btab01 was increased about two times by adding edible oil. When L. lecani Btab01 was treated to control nymph with 0.1% edible oil, it showed high control activity(98.6%) compared to single treatment of L. lecani Btab01 (79.9%).

• International Journal of Industrial Entomology and Biomaterials
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• v.14 no.2
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• pp.143-146
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• 2007

Concerns of entomopathogenic fungi as alternative pest control agents are increasing even though chemical pesticides have been used as the main control agents for pests and diseases in crop production. This study was conducted to test the influence of fungicides and insecticides on an isolate of Lecanicillium attenuatum that was reported to have the pathogenicity against cotton aphid, because fungicides and/or insecticides can apply with mycopesticides simultaneous, before and/or after. Fungicides fenbuconazole+thiram and propineb inhibited the spore germination and mycelial growth of L. attenuatum CS625; dimethomorph and procymidone did not affect spore germination or mycelial growth. The insecticide abamectin, deltamethrin, imidachropride, and spinosad had no detrimental effects on spore germination or mycelial growth. Therefore, these results demonstrated that careful selection of pesticides and fungicides can be applied to the integrated pest and disease control with microbial pesticide.