• Title/Summary/Keyword: Leaf weight

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Chitosan Stimulates Calcium Uptake and Enhances the Capability of Chinese Cabbage Plant to Resist Soft Rot Disease Caused by Pectobacterium carotovorum ssp. carotovorum

  • Jang, Eun-Jung;Gu, Eun-Hye;Hwang, Byoung-Ho;Lee, Chan;Kim, Jong-Kee
    • Horticultural Science & Technology
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    • v.30 no.2
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    • pp.137-143
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    • 2012
  • Chinese cabbage plant was grown hydroponically for 4 weeks in order to examine the temporal relationship of calcium concentration of the nutrient solution with calcium content in the leaf tissue and susceptibility of the tissue to soft rot disease by $Pectobacterium$ $carotovorum$ ssp. $carotovorum$ (Pcc). Calcium concentration from 0.5 to 32.0 mM was maintained for 1 week using Hoagland & Arnon solution. The calcium content of the leaf was proportionally increased to the concentration of the nutrient in the solution (r = 0.912). In contrast, the severity of soft rot symptom in the young leaves was inversely related with the amount of calcium supplied to the nutrient solution (r = 0.899). Water-soluble chitosan, prepared by hollow fiber filtration (> 100 kDa) was applied into the nutrient solution from 0.0 to 5,000 ppm. The chitosan of 10 ppm was the most effective to promote calcium uptake of the leaf, showing 155% of the control. The same chitosan solution prohibited most soft rot development of the leaf by Pcc, exhibiting only 53% of the control. Among different molecular weight fractions, chitosan fraction obtained from 30-100 kDa molecular weight cut-off promoted calcium uptake the most up to 163% of the control, and reduced the development of soft rot disease recording merely 36% of the control of the leaf tissue. The results obtained in the present study suggest that large scale production of water-soluble chitosan with an optimum molecular weight and its commercial application to Chinese cabbage production will be important to improve yield and quality of the crop.

The Effects of Mulberry Leaf-Jeolpyun on the Serum Lipid Level in Male University Students (뽕잎분말 첨가 절편 섭취가 남자 대학생의 혈청 지질 수준에 미친 효과)

  • 김애정;김명희;김성수;곽한병
    • Journal of the East Asian Society of Dietary Life
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    • v.10 no.5
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    • pp.387-393
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    • 2000
  • Recently, it appears to have a trend of adult having weight increase in Korea. The number of obese people is increasing in Korea due to the changes of meal pattern and the lack of physical activities. Currently, obesity is the prevalent health problem in the world. The purpose of this study was to investigate the effect of mulberry leaf-Jeolpyun on the serum lipids of overweighted male university students. After 3 days of education and adaptation on this study, twenty male university students with 20.9yrs old average were fed a uniformed dormitory foodservice, and they ate constant amount of 9% mulberry leaves added-rice cake, Jeolpyun(100g) instead of boiled rice(l00g) , on each meal. Experiment was conducted during 14 days period. All meals were provided promptly. and the subejects were required to have breakfast, lunch, and dinner on weekdays. Mean height, weight, and BMI were 176.55cm, 90.OOkg, and 28.73, respectively. Although it was not very significant, body weight of the subjects appeared to be reduced after consuming the 100g substitute diet of mulberry leaf-Jeolpyun. Daily energy Intake of the subjects was 2,360.6kcal, which represents 94% of the Korean RDA. The ratio of energy obtained from carbohydrate, protein, and fat was 55: 17'27 As compared with the Korean RDA, 65 : 15 20, carbohydrate consumption was decreased but protein and fat consumption was a little bit increased. The ratio of animal-protein source was 58%, and animal-fat intake was 42%. Triglyceride concentrations decreased. but HDL-cholesterol was in the reversed manner The concentrations of HDL-cholesterol increased significantly after the 2wk-lOOg subtitute diet of mulberry leaf-Jeolpyun (p<0.05) . However, there were no significant differences in total cholesterol and LDL-cholesterol con concentrations .

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Effects of Coriandrum Sativum L. on Lipid Metabolism in Rats with Hypertriglyceridemic Diet

  • Hwang, Geum-Hee;Heo, Young-Ran;Lee, Heung-Jae;Park, Ok-Ja;Kang, Seong-Koo;Kim, Yong-Doo
    • Nutritional Sciences
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    • v.4 no.1
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    • pp.13-19
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    • 2001
  • This study was designed to investigate the effects of Coriandrum Sativum L. on lipid metabolism in rats with hypertriglyceridemia. Also, we compared the effects of the leaf, seed and root of Coriandrum Sativum L. on lipid metabolism in rats with hypertriglyceridemia. Hypertriglyceridemia was induced in 32 male Sprague-Dawley rats weighting 108$\pm$13 g through the feeding of high-fat diets containing 20% fat and 5% cellulose for ten days. The rats were divided into four groups as follows : control (C), leaf (L), seed (S) and root (R) groups. For the five-week experimental period, the C group was fed the above diet an the L, S and R groups were fed Coriandrum Sativum L. diets containing 5% dry leaf, seed and root of Coriandrum Sativum L., respectively. Intake of diet and weight gain were significantly lower in the C group than in the L, S and R groups. But there was no significant difference among the L, S and R groups. Because of weight differences among the groups, all obtained data was adjusted to weight. The level of plasma total cholesterol was not significantly different among the four groups. But after adjusting to weight differences, the level of plasma triglyceride was significantly higher in the C groups than in the L and S groups. These results suggest that dietary Coriandrum Sativum L. may increase appetite and have an inhibitory effect on the lipid metabolism of rats with hypertriglyceridemia. Also, those effects may be partly different (leaf, root and seed) from those of Coriandrum Sativum L.

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Effect of Water and Ethanol Extracts of Persimmon Leaf and Green Tea Different Conditions on Lipid Metabolism and Antioxidative Capacity in 12-month-old Rats (추출 조건을 달리한 감잎과 녹차의 물 및 에탄올 추출물이 노령쥐의 지방대사와 항산화능에 미치는 영향)

  • 김성경;이혜진;김미경
    • Journal of Nutrition and Health
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    • v.34 no.5
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    • pp.499-512
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    • 2001
  • This study was performed to investigate effects of dried leaf powders, water, 75% and 95% ethanol extracts of persimmon leaf and green tea on lipid metabolism, lipid peroxidation and antioxidative enzyme activity in 12-month-old rats. Fifty-four male Sprague-Dawley rats weighing 542$\pm$4.5g were blocked into groups according to their body weight and were raised for four weeks with the diets containing 5%(w/w) dried leaf powders of persimmon(Diospyros kaki Thunb) and green tea(Camellia Sinensis O. Ktze), water or 75% and 95% ethanol extracts from same amount of each dried tea powder. Food intake was not significantly different among all groups, but weight gain of green tea powder group was significantly lower than that of control group. Plasma and liver lipid levels of all the tea diet groups were lower than those of control group. Especially, 75% ethanol extract of persimmon leaf decreased total lipid and triglyceride concentrations in plasma and 95% ethanol extract of persimmon leaf decreased liver total lipid level. However, there was no difference between 75% ethanol extracts groups and 95% ethanol extracts groups in lipid metabolism. Superoxide dismutase(SOD) and catalase activities in erythrocyte were remarkably increased by all the green tea diets. SOD, catalase and glutathione peroxidase activities in liver were increased by the feeding of ethanol extracts from green tea and persimmon leaf powder. Liver xanthine oxidase activity was not different among all groups. Plasma Thiobarbirutic acid reactive substance(TBARS) concentrations of all the green tea diet groups were significantly low. It was thought that high flavonoids in green tea inhibited plasma lipid peroxidation by promoting SOD, catalase activities in erythrocyte. 95% ethanol extract of persimmon leaf also inhibited plasma lipid peroxidation by high vitamin E and beta-carotene. Persimmon leaf powder decreased liver TBARS concentration by vitamin E, betacarotene and vitamin C and by increasing activities of antioxidative enzymes with flavonoids. In conclusion, dried leaf powders, water, 75% and 95% ethanol extracts of persimmon leaf and green tea were effective in lowering lipid levels and inhibiting lipid peroxidation in 12-month-old rats. Above all, ethanol extracts of persimmon leaf decreased plasma and liver lipid levels and persimmon leaf powder effectively inhibited liver lipid peroxidation. Extracts of green tea leaf inhibited plasma lipid peroxidation. In lowering lipid levels and inhibiting lipid peroxidation, ethanol extracts were more effective than water extracts, but there was no difference between 75% ethanol extracts and 95% ethanol extracts in lipid metabolism. (Korean J Nutrition 34(5) : 499~512, 2001)

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Anticancer Effect of Persimmon Leaf Extracts on Korean Gastric Cancer Cell (감잎의 물 및 에탄올 추출물이 한국인 위암 세포주에 미치는 항암효과)

  • Kim, Ho-Jung;Kim, Mi-Kyung
    • Journal of Nutrition and Health
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    • v.36 no.2
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    • pp.133-146
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    • 2003
  • This study was performed to investigate the in vitro and in vivo anticancer effects of persimmon leaf extracts on human gastric cancer cells. In vitro anticancer effects of persimmon leaf extracts (water extract at 8$0^{\circ}C$ for 3 hours, water extract at room temperature for 48 hours, 50% ethanol extract at 8$0^{\circ}C$ for 3 hours, 50% ethanol extract at room temperature for 48 hours, 75% ethanol extract at 8$0^{\circ}C$ for 3 hours and 75% ethanol extract at room temperature for 48 hours) on SNU16 (Korean gastric cancer cell) were investigated by MTT assay. Persimmon leaf extracts exhibited strong in vitro anticancer effects. We found that the higher the ethanol content of the solvent, the stronger the in vitro anticancer effects. Extraction yields, contents of flavonoids, vitamin A, vitamin C and vitamin E were measured. We found that the higher the ethanol content of the solvent, the higher the extraction yields and the contents of flavonoids, vitamin A and vitamin E. Among persimmon leaf extracts, 75% ethanol 8$0^{\circ}C$ extract showed the highest extraction yield, the highest contents of flavonoids, vitamin A and vitamin E and exhibitied the strongest in vitro anticancer effect on SNU16. Therefore, 75% ethanol 8$0^{\circ}C$ extract was chosen as the material to investigate in vivo anticancer effects. In vivo anticancer effect of persimmon leaf 75% ethanol 8$0^{\circ}C$ extract was investigated in SNU16 transplanted nude mice. Twenty five female nude mice (BALB/c) were blocked into five groups according to body weight and raised for 4 weeks with diets containing 4% (w/w), 8% (w/w) persimmon leaf 75% ethanol 8$0^{\circ}C$ extract, with IT (intratumoral) injection treatment with 1.65 mg/100 $\mu$1, 3.3 mg/100 $\mu$1 concentration every other day 3 weeks after SNU16 was transplanted. Persimmon 75% ethanol 8$0^{\circ}C$ extract significantly lowered tumor weight and tumor volume in SNU16 transplanted nude mice. Tumor weight and tumor volume in all experimental groups were significantly lower than those in the control group. Helper T cell (CD4) levels of mice injected with 3.3 mg/100 $\mu$1 extract significantly increased. Cytotoxic T cell (CD8) levels in all experimental groups significantly increased and helper/cytotoxic T cell ratios in all experimental groups significantly decreased. Natural killer cell and MHC class II molecule in all experimental groups significantly increased. In conclusion, persimmon leaf 75% ethanol 8$0^{\circ}C$ extract exhibited strong in vitro and in vivo anticancer effects against SNU16 cells and it increased cytotoxic T cell, natural killer cell and MHC classII molecule in experimental groups in SNU16 transplanted nude mice.

Effects of Planting Density and Fertilizer Level on the Growth, Yield, Quality and Nitrogenous Compounds of Burley Tobacco (재식밀도 및 시비량이 버어리종 잎담배의 생육, 수량, 품질 및 질소화합물에 미치는 영향)

  • Kim, Sang-Beom;Han, Chul-Soo;Ryu, Ik-Sang
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.32 no.1
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    • pp.40-47
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    • 1987
  • Under the different conditions of planting density and compound fertilizer level, some agronomic and chemical characteristics of burley tobacco were investigated from 1982 to 1984. Leaf area and dry leaf weight per plant, crop growth rate, relative growth rate and net assimilation rate for 40-60 days after transplanting were higher with increasing plant spacing and fertilizer, but leaf area index was lower with increasing plant spacing. At topping stage, the leaf size was increased with increasing plant spacing and amount of fertilizer applied and the stem diameter was increased by increasing plant spacing. Leaf area, leaf weight per plant and weight per unit leaf area of harvested leaf were higher when plant spacing and fertilizer increased. It was estimated that the optimum plant spacing was 105cm x 34cm and level of com-pound fertilizer (N-P$_2$ O$\sub$5/ -K$_2$O =10-10-20) was 263kg/l0a for high yield. There were trends toward increase the contents of total alkaloid and total nitrogen with increasing plant spacing and fertilizer application. There was significant positive correlation between plant spacing and total nitrogen, and between fertilizer application and total nitrogen. The plant spacing of 105cm x 35 to 40cm and 227.5kg/l0a of fertilizer level may be profitable for farm economy and the low nitrogenous leaf.

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Studies on Dry Matter Production and Variation of Agronomic Characteristics of Determinate and Indeterminate Types of Soybean Cultivars (Glycine max L.) Under Different Growing Condition (유ㆍ무한형대두품종의 재배조건에 따른 건물생산 및 형질변이에 관한 연구)

  • Keun-Yong Park
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.17
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    • pp.45-78
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    • 1974
  • To provide useful information for developing new high yielding soybean varieties and for improving cultural practices, an investigation was made on variation of dry matter production and on relationship among several agronomic characters of soybean plants grown under different planting times and densities as well as under different fertilizer levels, using Kwang-kyo, Dong puk-tae, and Suke # 51 as determinate types and Shelby, SRF-300 and Harosoy as indeterminate types at the Crop Experiment Station during the period of 1972 and 1973. The results obtained were summarized as follow: 1. The dry weight, CGR and LAI at the initial flowering stage were high in the high plant population irrespective of varieties, planting times, and fertilizer levels. However, those characters of the indeterminate type were lower than those of the determinate types. The same characters of the indererminate type at the terminal leaf stage were either same or higher than those of the determinate types. 2. The dry weight of the determinate type at the initial flowering stage was similar to the indeterminate, type, when planting times were May 21 or June 15. The dry weights of both types of varieties were low when planted on July 10. When fertilizer levels were increased, the CGR, dry weight and LAI at the initial flowering stages were also increased. 3. Even though significant differences of LAI were obtained among the varieties within the same plant type, the indeterminate type was in general lower than that of the determinate type regardless of planting time and densities, or fertilizer levels, while the yield of the indeterminate type was comparable to the yield of the determinate type. 4. The high degree of leaf- and petiole-fall at the greenbean stage was highly associated with early planting and high levels of fertilizers. However, less amount of leaf- or petiole-fall was found when planted on July 10 or under low plant population. 5. The percent of stem weight was high under higher plant population, while the percent of leaf weight was high under lower plant population. When planting time was late, the percent of stem and petiole weight were reduced, while the leaf weight was increased. 6. The percent of pod weight of the determinate type at the terminal leaf stage was about 2% when planted on May 21, about 8% when planted on June 15, and about 9% when planted on July 10. The percent of pod weight of the indeterminate type at the terminal leaf stage were about 6 % when planted on May 21, 14% when planted on June 15 and 21% when planted on July 10. 7. Kwang kyo showed less degree of leaf-fall even when lodged due to high levels of fertilizer applied, while SRF-300 showed great damage due to lodging. 8. High yields were obtained when planted on May 21, but there were little yield differences between yields from May 21 and June 15 plantings. The reduction of yield due to late planting of July 10 was less apparent in the determinate type of varieties, while it was high in the indeterminate type. 9. The optimum plant population per are for high yield was 1, 250 to 2, 500 plants when planted on May 21, 2, 500 plants when planted on June 15, and 3, 333 plants when planted on July 10. 10. High correlation coefficients were obtained between dry matter weight and LAI at the terminal leaf stages, and between the dry matter weight and yield at the greenbean stages. The optimum dry weight for high yield in the determinate type was expected to be 25 kg. per are at the initial flowering stage and 50 kg. per are at the terminal leaf stage. In the indeterminate type the LAI and dry weight at the greenbean stage were 4 to 5 and 80 kg. per are, respectively. 11. Under the high plant population plant height was increased, while the stem diameter and the number of nodes and branches were reduced. Consequently, the percent of mainstem to main stem plus branches were increased, and the length of internode was also elongated. The ratios of stem weight, number of nodes and pods, and yield of main stem were increased when high plant population was associated with the early planting. The percent of main stem to branches for the indeterminate type was higher than that of the determinate type. 12. Under the high plant densities and late planting, the percent of the pod number and yields of main stem were increased, indicating that varieties with no or less branches were better adaptable under such conditions. 13. High degree of simple correlation coefficients was obtained between the LAI at the initial flowering stage and terminal leaf stage, and the total node number, dry matter and dry stem weight of both determinate and indeterminate types. Even though no significant correlation was found between the LAI at the initial flowering stage of the determinate type and the stem length and pod number per are, highly significant correlation coefficients were obtained between such characters in the indeterminate type of varieties. 14. The dry matter was positively correlated with the LAI, CGR, stem length, and pod number, node number and dry stem weight per are, while no significant correlation was found between the dry matter and stem diameter. 15. The correlation coefficients between lodging index and the LAI, dry weight, stem length and dry stem weight were highly significant. Negative correlation was obtained for the indeterminate type between the stem diameter and lodging index. The correlation coefficient between the stem diameter and lodging index was non-significant for the determinate type, while positive correlation was obtained between the yield and lodging index in the determinate type. The lodging index was also positively correlated with average length of internode of main stem. 16. The 100 seed weight appeared to be lowered under the high plant population and no fertilizer condition, and when planted late. Apparent differences of 100 seed weight were found between main stem and branches, being higher for the main stem than for the branches. 17. No variation of protein content was found due to different cultural practices. However, the oil content was apparently reduced when planted late.

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Varietal Difference in Feed Value of Rice Straw and Its Relationship with Agronomic Traits (볏짚 사료가치의 품종간 차이 및 생육형질과의 관련성)

  • Kim Chang-Ho
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.49 no.6
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    • pp.516-521
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    • 2004
  • The straw of thirty one rice varieties were evaluated for their feed value and related agronomic traits. The rice straw were hand-harvested, dried to constant weight at $75^{\circ}C$ and ground through a 20 mesh seive in a Wiley mill, analyzed with crude protein (CP), acid detergent fiber (ADF) and neutral detergent fiber (NDF). Relative feed value (RFV) was calculated from NDF and ADF. The sum of standardized score was estimated by dry weight of rice straw, content of CP, ADF and NDF. The straw yield of Daeanbyeo was 725.9 kg/10a, showed heighest value among the varieties and remainder was in the order of Keumnambyeo, Donginbyeo #1 and Chucheongbyeo. Crude protein (CP) content in a Dasanbyeo was higher than those in other varieties. The content of ADF in a Junghwabyeo and NDF in a Sobaegbyeo were $34.3\%$ and $63.8\%$, respectively, showed lowest value among the varieties. The rice straw of Dunnaebyeo, Obongbyeo, Seoanbyeo, Keumobyeo, Hwaseongbyeo, Noganbyeo and Gyehwabyeo belonged to the high feed value varieties by estimation of cluster analysis, sum of standardized score and RFV. The content of CP was found to be positively related with dry weight of leaf and grain, but negatively related with heading days after seeding, culm length, specific leaf weight (SLW) and dry weight of stem. ADF and NDF were found to be positively related with heading days after seeding, culm length, SLW and dry weight of leaf, but negatively related with dry weight of stem. The sum of standardized score and RFV were the only positive relationship with dry weight of stem and negative relationship with other traits.

A Study on Factors Affecting Cigarette Weight (I) (제조담배의 중량변화 요인에 관하여(I))

  • 진학용;이태호
    • Journal of the Korean Society of Tobacco Science
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    • v.1 no.1
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    • pp.71-77
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    • 1979
  • The weight of domestic cigarette brands were examined by the three affecting factors, the standard leaf blending and the filling value of each leaf, the length of tobacco rod, and the apparent quality conditions. The calculated cigarette weight we obtained was 760mg, whereas the operation standard weight was established as 790mg ~820mg for the 60mm tobacco rod. In the case of the 64mm rod this value was estimated to be 820mg compared with the operation standard weight of 870mg ~ 900mg. For the 56mm rod it was calculated as 720mg being compared to the standard value of 770mg~ 780mg. These results means that the operation standard weight of the domestic brands can cut down by 5-9 per cent in weight.

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Effects of Media, Hormones, and PFP on the Proliferation of Red Callus in Leaf Tissue Cultures of Garden Orach(Atriplex hortensis L.) (Garden Orach 조직배양에서 적색 캘러스의 증식에 미치는 배지, 호르몬 및 PFP의 영향)

  • 이주경
    • Korean Journal of Plant Resources
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    • v.7 no.2
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    • pp.171-175
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    • 1994
  • The effects of medium, hormones, and PFP on the proliferation of red callus in leaf tissue cultures of Garden orach(Atriplex hortensis L.) was investigated. As a result,88% of leaf tissues formed eallus on MS nledium containing 2mg/$\ell$ 2,4-D. Fresh weight of callus was higher on MS medium than on Bsand NN media. It was also found that 2, 4-D was more effective than Dicamba and Picloram. The op-timum concentrations of hormones for callus proliferation depended on culture media. Isolated red cal-lus grew markedly both on MS medium supplemented with 1-2mg/$\ell$ 2, 4-D and Bs medium contain-ing 2-4111g/$\ell$ 2,4D. Callus proliferated on B5 and NN media containing Dicabma Img/$\ell$ as well as onthe same media containing 2mg/$\ell$ Picioram. The addition of PFP concentrations of 2, 5, and 40mg/ $\ell$rcspectiely to culture medium caused increase of callus fresh weight, especially under light condition.

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