• Korean Journal of Weed Science
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• v.18 no.3
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• pp.205-213
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• 1998

This experiment was carried out to produce herbicide resistant potatoes hawing only chimeric phosphinothricin acetyltransferase (PAT) genes without using antibiotic selectable marker. The pDY502 vector having only PAT gene was reconstructed for transformation of potato. The reconstructed vector was introduced to Agrobacterium tumefaciens MP90 disarmed, and they were used for potato transformation. Hormonal requirement for plant regeneration from leaves and stem explants of potato was investigated. From this experiment, MS medium treated with IBA 0.1 mg/L + BA 0.5 mg/L was the best for potato regeneration, and the ratio of shoot regeneration was 54% for leaf and 46% for stem in that condition. For transformation, explants of potato leaves and stems were cocultured with A. tumefaciens MP90 containing reconstructed vector harvoring only PAT gene. When the potato explants were placed on various concentrations of bialaphos and all the potato explants were dead on medium with over 5.0mg/L bialaphos. By this selection methods, the explants cocultured with Agrobacterium produced the putative transgenic shoots on medium with 5mg/L bialaphos treatment after 3-4 weeks. Second selection was performed by transferring the shoot tips of putative transgenic to medium containing 20mg/L of bialaphos. The shoot tips grew well on the second selection medium, indicating the production of successful transgenic plants. But normal shoots were dead in same cytotoxic medium. Incorporation of the PAT gene into transgenic potatos were confirmed by PCR analysis of DNA and Southern hybridization. These results show that the PAT gene can serve as a selectable marker and herbicide resistant genes for transformation of potato.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.32 no.2
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• pp.201-207
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• 1987

This experiment was conducted to identify the superior corn hybrids for silage production and to determine the combining ability of their parental inbred lines. A 7-entry diallel cross was evaluated for grain and stover yields, and agronomic characters under 3 different seasons. Hybrids Hi34 x Tx601, Hi26 x Hi34, and Hi29 x Hi34 showed high total dry matter yields. In general, late maturing hybrids had higher grain and silage yields than early maturing hybrids. Leaf area index was correlated with grain and stover yields. Rust rating was negatively correlated with yields and all characters, indicating that rust (Puccinia sorghi) was one of the major factors affecting yields. Diallel analysis showed that inbred Hi34 which was late in maturity and resistant to rust was the best combiner for both grain and silage yields. General combining ability (GCA) and specific com-bining ability (SCA) effects for yields and other characters were significant, suggesting that both additive and non-additive gene effects are involved for those characters. Ratio of GCA/SCA mean squares showed that GCA effects were more important than SCA effects for yields and other characters. GCA x season and SCA x season interactions were significant for grain and stover yields, revealing that gene effects were not stable for those yields under dissimilar environments.

• Korean Journal of Agricultural and Forest Meteorology
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• v.17 no.3
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• pp.217-226
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• 2015

To investigate the distributions of BVOCs (Biogenic Volatile Organic Compounds) from mountain near mega city and their role in forest atmospheric, BVOCs and their oxidized species were measured at a 41 m tower in Mt. Taehwa during May, June and August 2013. A proton transfer reaction-mass spectrometer (PTR-MS) was used to quantify isoprene and monoterpenes. In conjunction with BVOCs, $O_3$, meteorological parameters, PAR (Photosynthetically Active Radiation) and LAI (Leaf Area Index) were measured. The average concentrations of isoprene and monoterpenes were 0.71 ppbv and 0.17 ppbv, respectively. BVOCs showed higher concentrations in the early summer (June) compared to the late summer (August). Isoprene started increasing at 2 PM and reached the maximum concentration around 5 PM. In contrast, monoterpenes concentrations began to increase 4 PM and stayed high at night. The $O_3$ maximum was generally found at 3 PM and remained high until 5 PM or later, which was concurrent with the enhancement of $O_3$. The concentrations of BVOCs were higher below canopy (18 m) than above canopy, which indicated these species were produced by trees. At night, monoterpenes concentrations were negatively correlated with these of $O_3$ below canopy. Using MEGAN (Model of Emissions of Gases and Aerosols from Nature), the emissions of isoprene and monoterpenes were estimated at 1.1 ton/year and 0.9 ton/year, respectively at Mt. Taehwa.

• Journal of Bio-Environment Control
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• v.14 no.4
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• pp.284-288
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• 2005

This study was conducted to clarify the effects of supplying methods of selenium on the growth and Se uptake of hydroponically grown tomato plants. Tomato seeds (Lycopersicum esculentum Mill. cv. Momotaro T-93, Daki Seed Co.) were sown in plug tray with fifty holes, and raised for sixty days. Tomato seedlings transplanted to coco fiber slabs were supplied with the nutrient solutions adjusted to EC $2.3dS{\cdot}m^{-1}$ and pH $5.8\~6.2$ recommended by the Japanese Horticultural Experiment Station. Selenium forms used were inorganic $SeO_2$ (here in after referred to Se) and organic selenium chlenium with sugar fatty acid ester (here in after referred to chelated-Se). 10 ppm selenium solutions were treated to tomato plants with foliar applications, drenching, and foliar application plus drenching. Growth characteristics in terms of plant height, number of leaves, leaf area and chlorophyll content were significantly increased in the plot of foliar application ot Se, and in the plot of foliar application plus drenching of chelated-Se than other plots, respectively. Transported contents of selenium into the tomato fruits were highest as 0.302 ppm in the plot of foliar application plus drenching of chelated-Se. Also, it had tended to be higher in the plot of foliar application plus drenching than in the plots of foliar application or drenching in both of Se and chelated-Se. Foliar application and drenching of organic chelated-Se were effective to produce the functional tomato fruits.

• Korean Journal of Food Science and Technology
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• v.52 no.3
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• pp.226-236
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• 2020

This study was designed to evaluate the biological activities and main constituents of different parts (fruit, leaf, and stem) of aronia (Aronia melanocarpa). The total phenolic and flavonoidcontents, DPPH and ABTS⁺ radical-scavenging activity, reducing power, and ferric reducing/antioxidant power were observed to follow the order of: leaves > stems > fruits, regardless of extraction solvents. The inhibitory activity against lipopolysaccharide-induced NO production in Raw 264.7 cells was significantly higher in the aronialeaf extract-treated group than in the groups treated with stem and fruit extracts. The ultra-performance liquid chromatography (UPLC) analysis was mainly composed of routine. In addition, the highest content level was measured in the case of the catechinmemberepigallocatechin witha higher value than that found in green tea. Theresults of this studyprovide useful information for understanding the chemical constituents and biological activities of aroniafruits and byproducts.

• Horticultural Science & Technology
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• v.28 no.5
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• pp.728-734
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• 2010

We studied the accumulation and partitioning of dry weight (DW) and nitrogen (N) in different parts of field-grown 'Fuyu' persimmon to elucidate that the foliar applications of supplemental N in June or September compared favorably with the traditional soil application in securing leaf area and fruit production. We also estimated the proportion of N permanently removed from the tree at the end of a growing season. Urea was applied either to leaves in June and/or September or to the soil in June and September for three consecutive years, and the trees were excavated in November for analyses. Total DW ranged from 4.2-4.8, 8.7-9.2, and 17.1-21.5 kg in a 4-, 5-, and 6-year-old tree, respectively, without statistical difference among the four treatments. Of the total DW, 3.3-10.2% was in shoots, 5.7-10.5% in leaves, 8.3-31.4% in aerial woods, 13.0-27.0% in root, and 28.0-59.3% in fruits. As the trees became more productive, DW proportion of fruits significantly affected that of the root: in 6-year-old trees, root DW accounted for only 10.6-15.8% of the tree total when fruit DW accounted for 50-60%. N contents ranged from 24.6-28.3, 48.3-53.5, and 98.3-122.6 g in a 4-, 5-, and 6-year-old trees, respectively, without statistical difference among the treatments. Of the total N, 6.2-11.5% was in shoots, 16.7-24.3% in leaves, 17.6-23.5% in aerial woods, 17.2-37.5% in roots, and 16.9-34.4% in fruits. As in DW, the increase in the proportion of N in fruits decreased in the root most significantly. Application methods for supplemental N did not affect the proportion of DW and N removed from the tree through abscising leaves and harvested fruits. Percentage of DW removal was 41 in 4- and 5-year-old trees, but it was 61 in more productive 6-year-old trees; that of N was 39, 43, and 49%, respectively. No significant changes in the contents of DW and N in field-grown trees, as well as their percentages removed from the tree at the end of the season, demonstrated that foliar application of supplemental N was as good as soil applications with much less N.

• Korean Journal of Plant Resources
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• v.25 no.1
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• pp.156-168
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• 2012

Chrysanthemum is a cut flower species that normally lasts for 1 to 2 weeks, in some cases 3-4 weeks. This has been attributed to low ethylene production during senescence. Reduction in cut flower quality has been attributed to the formation of air embolisms that partially or completely blocks the water transport from the vase solution to the rest of the cut flower stem, increasing hydraulic resistance which may cause severe water stress, yellowing, wilting of leaf, and chlorophyll degradation. Standard type chrysanthemum can be harvested when buds were still tightly closed and then fully opened with the simple bud-opening solution. Standard type chrysanthemum can also be harvested when the minimum size of the inflorescence is about 5-6 cm bud which opened into the first flower full-sized flower. While spray varieties can be harvested when 2-4 most mature flowers have opened (40% opening). Cut flowers are sorted by stem length, weight, condition, and so on. Standard chrysanthemum is 80 cm length for standard type and 70cm for spray type. Pre-treatment with a STS, plant regulator such as GA, BA, 1-MCP, chrysal, germicide, and sucrose, significantly improved the vase life and quality of cut flowers. It is well established that vase solutions containing sugar can improve the vase life of cut chrysanthemum. Chrysanthemum is normally packed in standard horizontal fiberboard boxes. Chrysanthemum should normally be stored at $5{\sim}7^{\circ}C$. Precooling resulted in reduction in respiration, decomposition, and transpiration activities as well as decoloration retardation. There was significant difference between "wet" storage in 3 weeks and "dry" storage in 2 weeks. In separate pulsing solution trials, various germicides were tested, as well as PGRs to maintain the green color of leaves and turgidity. Prolonging vase life was attained with the application of optimal solution such as HQS, $AgNO_3$, GA, BA and sucrose. This also retarded senescence in leaves of cut flower stems. Fresh cut chrysanthemum can be transported using a refrigerated van with $5{\sim}7^{\circ}C$. Increasing consumption and usage of cut chrysanthemum of various cultivars would require efficient transport system, and effective information exchange among producer, wholesaler, and consumer.

• Journal of Bio-Environment Control
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• v.17 no.4
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• pp.288-292
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• 2008

This study was conducted to identify the effects of irrigation amount to produce high quality melon fruit in fertigation culture. Irrigation amount of during fruit harvesting period was doubled at the low irrigation point ($(-45{\sim}50\;kPa$) treatment as 115 mm as than that of the high irrigation point ($-20{\sim}25\;kPa$) treatment. The plant growth rates such as stem length, leaf weight and plant height were a little diminished at the low irrigation point ($-45{\sim}50\;kPa$) than those of the other treatments. Internode length was however not affected by irrigation amount. Fruit weight was lighter at the low irrigation point ($-45{\sim}50\;kPa$) than that of at the high irrigation point and fruit height was shorter, but fruit diameter was not affected by irrigation amount. Fruit soluble solid was $0.9^{\circ}Bx$ higher at the low irrigation point ($-45{\sim}50\;kPa$) than at the high irrigation point ($-20{\sim}25\;kPa$) and net index was higher. Total marketable yield was highest by 3,937 kg/10a at the high irrigation point ($-20{\sim}25\;kPa$), but the excellent marketable yield was highest by 2,531 kg/10a at the low irrigation point ($-45{\sim}50\;kPa$). Inorganic contents of the soil N, K, Ca and Mg were not affected by irrigation amount. It was therefore thought that optimum irrigation point to produce high quality melon fruit by fertigation culture was $-45{\sim}50\;kPa$ at ripening stage.

• Korean Journal of Environmental Agriculture
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• v.6 no.1
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• pp.67-75
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• 1987

The major objectives of this study were to find out the growth and yield of two ecotypes of Korean garlic in main garlic producing districts in Korea. The data were collected by the field survey which had been conducted at 270 township in 58 major garlic production countries throughout the country on 10th, 20th, and 30th day of every month from 1982 to 1984. The results of this study were as follow: 1) Sowing period of garlic of southern and northern ecotype were around September 20 and October 20, respectively. 2) Average number of plants per $3.3m^2$ of the southern and northern ecotype were 123 and 100, respectively. 3) Leaf emergence time of southern ecotype was before the beginning of winter (November) and that of northern ecotype was from early February till April. 4) Stepwise multiple regression analysis showed that the plant heights measured on November 30 for southern ecotype and on June 30 for northern ecotype most adequately predicted the yield of garlic. The relationship between yield and plant height were as follows: Southern ecotype; Y=571.56+5.34X Northern ecotype; Y=251.81+5.45X where Y is yield expressed in Kg/l0a and X is height in cm at the respective date. 5) The number of leaves increased until harvest in both ecotypes. At harvest, the number of leaves in southern and northern ecotype were 10 and 8, respectively. Number of leaves counted on January 20 for southern ecotype and on June 20 for northern ecotype correlated best with the yield of the ecotypes. 6) The highest senescent portion of southern ecotype and northern ecotype were seen on January 30 and May 30, respectively. Stepwise multiple regression analysis showed that the senescent portion of southern ecotype counted on January 30 and that of northern ecotype on June 20 mostly affected the yield. 7) Average yield of southern and northern ecotype at the main garlic producing districts were 771 and 652Kg/10a, respectively.

• Journal of The Korean Society of Grassland and Forage Science
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• v.32 no.1
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• pp.15-28
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• 2012

This study was carried out to know adaptability and growth characteristics, yield, chemical compositions and nutrition yield of corn hybrids for silage at paddy field. The field experiments were conducted at Sangju province for one year (2009). The experimental design was arranged in a randomized block design with three replication. The treatments consisted of eleven corn hybrids. The planting date was on 1 May and harvested at 24 August. Stem diameter, stem hardness and number of ear were higher in P32P75 than other varieties. Ear height, dead leaf and green degree were highest in $NC^+$7117, but number of root system and Brix ($B^{\circ}$) were higher in P3394 than other varieties. Crude protein and crude fat (EE) were highest in P32K61 and P31P41, respectively (P<0.01). NDF and ADF were highest in KPO and KIO, respectively, but no significant differences were found among the varieties. Total mineral contents were the highest in Kwangpyongok (9,775 mg/kg), and P3394 (6,651 mg/kg) was the lowest as compared to other varieties (P<0.01). Crude protein yield, crude fat yield and mineral yield were highest in P3156, P31P41 and KPO, respectively (P<0.01). Total composition amino acid and total fatty acid were the highest in P32K61 and KIO, respectively (P<0.01). Yields of crude protein, fatty acid, composition amino acid and TDN were the highest in P3156 (P<0.01). But yields of crude protein and mineral were the highest in P31P41 and KPO, respectively (P<0.01). Total digestible nutrient (TDN) was higher in order of P3156 > $NC^+$7117 > P31N27 > KPO > P32K61 > P32T83 > P32P75 > P31P41 > P3394 > P32W86 > KIO. Based on the above results, corn hybrid varieties could be recommended in P3156, NC+7117 and P31N27 for growth characteristics, quantitative production and nutrition yield.