• 한국염색가공학회지
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• 제21권1호
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• pp.10-20
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• 2009

The objective of this study is to investigate the efficacy of leaf powder colorants as substitutes for traditional fresh juice extract dyeing. Three kinds of leaf powder colorants were prepared by freeze drying method with or without deep freezing as pre-treatment: one powder colorant from fresh leaf juice with deep freezing; two kinds of powder colorant from fresh leaves with and without deep freezing. Their dyeing properties and storage stabilities were studied and compared with the traditional fresh juice extract dyeing. The presence of indigo in the powder colorants was confirmed by UV/Visible absorption spectra. They showed absorption peak at 602nm which was same with indigo absorption peak. Dyeing was done at low temperature around 6$^{\circ}C$. All three powder colorants produced B colors on silk fabrics, showing similar color to the one dyed traditionally with fresh juice extract. The powder colorants from leaves gave higher color strength than the powder from leaf juice. The powder colorant prepared from leaves with deep freezing was the most stable for long term storage as its color and color strength were not changed after 360 days. So, this was used for further dyeing to study the effects of concentration and repeat dyeing on color strength and colorfastness. Fastnesses to dry cleaning and rubbing were fairly good above 4 rating. Further study is needed to improve light fastness. It was concluded that the leaf powder colorant with deep freezing could be used as a substitute for traditional juice extract dyeing at all seasons.

• 한국염색가공학회지
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• 제21권4호
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• pp.23-32
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• 2009

The objective of this study is to investigate the efficacy of leaf powder colorants as substitutes for traditional indigo dyeing. Leaf powder colorants were prepared by hot air($50^{\circ}C$) and room temperanrre($25^{\circ}C$) drying methods from fresh leaves. The presence of indigo in the leaf powder colorants was confirmed by UV/Visible absorption spectra. All the powder colorants showed broad absorption at 602 nm as same as synthetic indigo. Dyeing was done by reduction method with sodium hydrosulfite and sodium hydroxide. Leaf powder colorants produced blue color on silk fabrics, showing similar color to the one dyed traditionally with fresh juice extract. The powder colorants prepared at room temperature drying were more stable for long term storage than that prepared by hot air drying. Thus, the powder colorants prepared by room temperature drying was reduced and dyed in one-step process without sodium hydroxide in the dyebath for further investigate dyeing properties. K/S value of the fabric dyed without sodium hydroxide was much higher than one dyed with sodium hydroxide. Regardless of the addition of sodium hydroxide, rubbing fastness was fairly good showing above 4 rating. Fastness to dry cleaning and light of the fabrics dyed without sodium hydroxide were mote higher than that dyed in alkaline condition.

• Journal of Ginseng Research
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• 제13권2호
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• pp.158-164
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• 1989

인삼엽소병(leaf-burning disease) 원인과 light-harvesting chlorophyll-protein(LHCP) complex의 solar energy 분배능력과의 상호 연관성을 조사하기 위한 기초 연구로써 인삼 thylakoid의 chlorophyll-protein(CP) complex의 조성 및 특징을 조사하였다. 인삼의 CP-complex는 non-denaturing SDS-PAGE 방법에 의해 4개 bands로 분리되었으며 각 band는 Bassi와 Dunahay의 결과에 따라 CPI(PSI의 reaction center와 LHCP I antennae), CP I(PSI reaction center), LHCP II(LHCP II)의 oligoform), 그리고 LHCP II(PS II antennae; CP29, CP26)로 확인되었다. 인삼의 LHCP II 는 양지식물인 spinach, soybean과 비교해 볼 때 오히려 인삼의 band intensity가 더 높았으며, CP I band는 인삼에서만 분리되었다. 인삼 CP-complex band의 absorption 및 fluorescence spectra, chlorophyll a.b ratio 에서도 비교식물과 차이를 나타내었다. Thylakoid membrane의 polypeptide 함량은 인삼에서 비교식물에 비해 현저히 낮은 polypeptide 함량은을 나타내었다. SDS-PAGE에 의한 polypeptide pattern은 band의 수나 band intensity에서 비교식물과 차이를 나타내었으며, 특히 29-35 kD, 55 kD과 60 kD 근치에서 현저한 band intensity 차이를 확인하였다. Specific $^1O_2$에 의해 chl. a가 60%, chl.b는 90%, 그리고 carotenoid는 70%가 파괴되는 것으로 확인되었다.

• 한국약용작물학회지
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• 제14권4호
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• pp.244-249
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• 2006

다양한 광환경에 대한 음나무 유묘의 생장특성과 엽록소 함량, 파장별 흡광도, 광합성 속도 등 광합성 특성을 조사하였다. 엽면적, 엽병길이 , RGR은 낮은 광조건에서 생장시킨 개체에서 크게 나타났고, SLW는 강한 광조건에서 각각 생장한 개체에서 높았다. 엽록소 함량은 염록소 a, 엽록소 b, 엽록소 a+b 함량 등은 낮은 광조건에서 생장시킨 개체에서 높았고, 엽록소 a/b 율은 강한 광조건에서 각각 생장한 개체에서 높았다. 광합성 유효파장역에서 평균 흡광값은 음엽이 0.75로 양엽의 0.70보다 높았다. 광합성 속도는 강한 광조건에서 생장한 개체가 높은 광합성 속도를 나타내었다. 광포화점은 전광조건 하에서 생육시킨 유묘에서 $700\;{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, 임내광 조건에서 생육시킨 조건에서 $300\;{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$이었고, 생장상의 강광조건에서는 $500{\;}{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, 약광조건에서는 $300{\;}{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$이었다.

• 한국환경농학회지
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• 제13권2호
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• pp.175-182
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• 1994

색비름(Amaranthus tricolor L.) 유식물에 인공산성비 pH 2.0, 3.0, 4.0, 5.0 및 수도물(pH 6.5)을 엽면처리하여 생장, 색소 및 엽표면의 형태에 미치는 영향을 조사하였다. pH 3.0 이하에서는 지상부 및 근부가 다같이 생장이 심하게 억제되었으며 잎에는 괴사반점이 나타났다. 색소의 흡광 spectrum은 7개의 peak가 나타났는데 Early splendor는 473nm과 535nm에 peak가 없었고, Tricolor는 476nm 및 546nm에 peak가 보이지 않았다. 강산성비는 식물색소 발현에 관여하였고 엽록소는 pH가 낮을수록 증가하였다. 주사현미경에 의한 엽표면 관찰에서는 pH 5에서도 이상이 관찰되기 시작하고 pH 4 이하에서는 괴사가 급격히 진행되고 있었다.

• 한국토양비료학회지
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• 제12권2호
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• pp.109-116
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• 1979

Ammonia 가스를 $0.15mg/{\ell}$, $0.25mg/{\ell}$, $0.5mg/{\ell}$로 대두(大豆)의 영양생장기에 각(各) 1시간(時間)씩 접촉(接觸)시켰고, $0.2mg/{\ell}$의 Ammonia 가스를 1일중시각별(日中時刻別)로 06:00-07:00, 10:00-11:00, 14:00-15:00, 18:00-19:00, 22:00-23:00시(時)에 각각(各各) 1시간(時間)씩 접촉(接觸)시켜 대두(大豆)의 수량(收量), 피해증상, 피해엽율(被害葉率), 체내질소 및 엽록소 함량(含量)을 조사(調査)한 결과(結果)는 다음과 같다. 가. Ammonia 가스농도(濃度)가 증가함에 따라서 유의성(有意性)있는 피해엽율(被害葉率) 및 감수율증가를 가져왔고, 주간(晝間)에 접촉(接觸)한 것이 야간(夜間)에 접촉(接觸)한 것보다 감수(減收)가 컸다. 나. 피해엽율(被害葉率)과 수량간(收量間)에는 고도(高度)의 유의성(有意性) 있는 부(否)의 상관($r=-0.9553^{**}$)이 있었다. 다. $0.2mg/{\ell}$의 Ammonia 가스는 14:00-15:00시(時)에 접촉(接觸)한 것이 피해엽율(被害葉率)이 가장 컸으며 22:00~23:00시에 접촉(接觸)한 것이 가장 적었다. 라. Ammonia 가스농도증가에 따라 식물체내 전질소(全窒素) 및 수용성질소함량(水溶性窒素含量)은 증가하는 경향(傾向)이고 엽록소함량은 감소(減少)하는 경향(傾向)이었으며 엽록소 a는 b보다 감소정도(減少程度)가 컸다. 마. 체내질소함량은 오후(午後)에 접촉(接觸)한 것이 오전(午前)에 접촉(接觸)한 것보다 많았으며 엽록함량은 야간(夜間)에 접촉(接觸)한 것이 주간(晝間)에 접촉(接觸)한 것보다 많았다. 바. 엽록체의 Spectra는 10~11시(時)에 가장 감소(減少)가 컸고 22~23시(時)에 가장 감소(減少)가 적었다.

• 한국염색가공학회지
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• 제20권3호
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• pp.18-24
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• 2008

The purpose of this study is to develop the dyeing method with dry leaves of indigo plant. Coloring matter was extracted from dry leaves of indigo plant with hot sodium hydroxide solution. The extract was reduced with sodium dithionite, and it was used for dyeing cotton fabrics under various conditions. UV-visible absorption spectra of extract, reduction rate of extracts by reducing agent, and the surface color of dyed cotton, lightfastness were examined. For the initial 20 minutes, the absorbance of indigo solution rapidly decreased. However, several hours later, the decreasing rate retarded. By repeating the dyeing process, the shade looked deeper and deeper. At $30-40^{\circ}C$, the value of K/S reached the highest point. The concentration of indigo solution in dye bath seemed to playa critical role for the reaction of the reducing agent. It was observed that the surface color of cotton fabrics was getting bluish and its degree of value and chroma seemed slightly decreased as the K/S value was increasing. The lightfastness was clearly enhanced by increasing the K/S value.

• Journal of Plant Biology
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• 제25권3호
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• pp.135-151
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• 1982

This investigation was carried out to clarify the changes of pigments and soluble protein, and photosystem II activity in the leaves of barley (${SO}_2$-sensitive) and corn (${SO}_2$-resistant) seedlings induced by the ${SO}_2$ fumigation (10, 50ppm). The pH changes of the leaf extract, the content of sulfite and sulfate, the activities of catalase, peroxidase, and polyphenoloxidase were compared in the leaves of barley and corn seedlings induced by ${SO}_2$ fumigation. The results are summarized as follows: An appreciable effect of pH change of leaf extract by ${SO}_2$ fumigation was observed in barley leaves (pH 6.10 to 5.18), but only a small change occurred in corn leaves (pH 5.66 to 5.50). The same pattern of pH changes was recorded when the solution of 0.2N HCl was added to leaf extract, providing lower buffering capacity of the barley leaves than corn leaves. After 2 hours of exposure to 10 ppm ${SO}_2$, the contents of ${SO}^{2-}_3$ and ${SO}^{2-}_4$ were increased in barley leaves, while only ${SO}^{2-}_4$ increased in corn leaves. After fumigation with 10ppm ${SO}_2$ for 2 hours, barley leaves showed significant decreases in activities of catalase, to 17% peroxidase, to 58%, and polyphenoloxidase, to 88%. Corn leaves showed increases in activities of peroxidase, to 136%, and polyphenoloxidase, to 128%. Absorption spectra of pigments obtained from ${SO}_2$-fumigated leaves were gradually decreased with the fumigation time increases, but the decrease was more significant in barley leaves. Fumigation with 50ppm ${SO}_2$ for 2 hours induced the greatest decomposition in carotenoid, followed by chlorophyll a and then chlorophyll b in barley leaves. The ratio of chlorophyll a/b was decreased from 4.1 to 3.6 in barley leaves, but in corn leaves it was maintained almost a constant level(4.9-4.8). The rate of decomposition of chlorophyll and carotenoid in corn leaves was very slow than those in the barley leaves. Fumigation with 50 ppm ${SO}_2$ for 2 hous, decreased the protein content of barley leaves to 59%, and that of corn leaves to 89%, and the extent of decrease in protein content was greater than that of pigments in barley and corn leaves. The rate of DCIP9dichlorophenol indophenol) photoreduction in ${SO}_2$-fumigated leaves was decreased to 18 and 67% in barley and corn leaves, respectively. However, DCIP photoreduction was considerably recovered about 32 and 92% with the addition of DPC(diphenylcarbazide) as an exogenous electron donor in barley and corn leaves, respectively.