Abstract
This experiment was conducted to find out the effects on soybean plant exposed with various concentration and different fumigation periods of ammonia gas ($0.2mg/{\ell}$). The yield of soybean, nitrogen and, chlorophyll contents in leaves, and percentage of destroyed leaf area were investigated. The results were summarized as: 1. The soybean yield losses and percentage of destroyed leaf area were positively correlated with concentration of inflicting ammonia gas respectively. The yield losses was higher at noon exposure time than at night exposure time. 2. The soybean yield was negatively correlated with the percentage of destroyed leaf area. 3. The highest percentage of destroyed leaf area had at afternoon exposure time (14:00-15:00 o'clock) by $0.2mg/{\ell}$ ammonia gas fumigation, and the lowest one had at midnight exposure time (22:00-23:00 o'clock). 4. According to the increasing concentration of ammonia gas, the total and water soluble nitrogen contents in soybean leaves were increased, but the contents of chlorphyll b on decreasing rate. 5. The nitrogen contents in plant were higher in the afternoon exposure than in the morning, but the contents of chlorophyll were higher at night time exposure than at day time. 6. The highest decreasing of absorption spectra of chlorohpyll in chloroplast had at 10:00-11:00 o'clock on fumigation time and the lowest one had at 22:00-23:00 o'clock.
Ammonia 가스를 $0.15mg/{\ell}$, $0.25mg/{\ell}$, $0.5mg/{\ell}$로 대두(大豆)의 영양생장기에 각(各) 1시간(時間)씩 접촉(接觸)시켰고, $0.2mg/{\ell}$의 Ammonia 가스를 1일중시각별(日中時刻別)로 06:00-07:00, 10:00-11:00, 14:00-15:00, 18:00-19:00, 22:00-23:00시(時)에 각각(各各) 1시간(時間)씩 접촉(接觸)시켜 대두(大豆)의 수량(收量), 피해증상, 피해엽율(被害葉率), 체내질소 및 엽록소 함량(含量)을 조사(調査)한 결과(結果)는 다음과 같다. 가. Ammonia 가스농도(濃度)가 증가함에 따라서 유의성(有意性)있는 피해엽율(被害葉率) 및 감수율증가를 가져왔고, 주간(晝間)에 접촉(接觸)한 것이 야간(夜間)에 접촉(接觸)한 것보다 감수(減收)가 컸다. 나. 피해엽율(被害葉率)과 수량간(收量間)에는 고도(高度)의 유의성(有意性) 있는 부(否)의 상관($r=-0.9553^{**}$)이 있었다. 다. $0.2mg/{\ell}$의 Ammonia 가스는 14:00-15:00시(時)에 접촉(接觸)한 것이 피해엽율(被害葉率)이 가장 컸으며 22:00~23:00시에 접촉(接觸)한 것이 가장 적었다. 라. Ammonia 가스농도증가에 따라 식물체내 전질소(全窒素) 및 수용성질소함량(水溶性窒素含量)은 증가하는 경향(傾向)이고 엽록소함량은 감소(減少)하는 경향(傾向)이었으며 엽록소 a는 b보다 감소정도(減少程度)가 컸다. 마. 체내질소함량은 오후(午後)에 접촉(接觸)한 것이 오전(午前)에 접촉(接觸)한 것보다 많았으며 엽록함량은 야간(夜間)에 접촉(接觸)한 것이 주간(晝間)에 접촉(接觸)한 것보다 많았다. 바. 엽록체의 Spectra는 10~11시(時)에 가장 감소(減少)가 컸고 22~23시(時)에 가장 감소(減少)가 적었다.