• 한국식품영양과학회지
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• 제33권3호
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• pp.560-565
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• 2004

배추김치로부터 식품유해균인 Listeria monocytogenes를 저해하는 박테리오신을 생산하는 유산균, Lactobacillus sakei P3-1이 분리되었다. 형태학적, 생화학적 특성조사와 최종적으로 PCR로 증폭하여 얻은 16S rDNA 염기서열 결정을 통해서 L. sakei로 동정되었다. L. sakei P3-1이 분비하는 박테리오신은 여러 그람 양성 및 음성균들 중에서 단지 L. monocytogenes만을 저해하는 그래서 저해범위가 매우 좁은 박테리오신으로 확인되었다. 이온교환 크로마토그래피에 의해서 박테리오신은 부분 정제되었으며 박테리오신의 열처리 안정성을 조사한 결과 121$^{\circ}C$에서 15분간 그리고10$0^{\circ}C$에서 10분간 열처리 후에도 각각 12.5%와 50%의 역가가 잔존하여 상당한 열안정성을 지니고 있음을 알 수 있었다. MRS배지에서 배양중 배양온도가 박테리오신 역가에 미치는 영향을 조사한 결과 3$0^{\circ}C$에서 배양할 때 그리고 18시간 이상 배양에서 가장 높은 1,000 AU/mL 역가를 보였다. 한편 SDS-PAGE 및 activity staining에 의해 측정된 박테리오신의 분자량은 4,000이었다. L. monocytogenes 생육 억제능, 작은 분자량 및 높은 열안정성 등의 성질들을 종합적으로 고려할 때 L. sakei P3-1이 생산하는 박테리오신은 박테리오신들 중에서 class II-a에 속하는 것으로 추정된다.

• Journal of Dairy Science and Biotechnology
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• 제36권3호
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• pp.146-154
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• 2018

There are more than 25 species of Mentha plants, which are aromatic perennial herbs. Currently, these species are being widely used with great interest because of various clinical findings regarding their health benefits. This is due to the abundance of volatile compounds that could expedite environmental interactions such as protection against herbivores, parasites, pathogens, and so on. Therefore, in this study, the antimicrobial effect of Mentha piperita (peppermint) oil on Bacillus cereus, Staphylococcus aureus, Cronobacter sakazakii, and Salmonella Enteritidis were investigated using the spot-on-lawn method. The results show that Mentha piperita (peppermint) oil exhibited antimicrobial activities against Bacillus cereus, Staphylococcus aureus, and Cronobacter sakazakii; however, it did not inhibit the growth of Salmonella Enteritidis. This shows that the antimicrobial effect of Mentha piperita (peppermint) oil is effective against both Gram-positive and Gram-negative bacteria. Hence, in the present study, Mentha piperita (peppermint) oil was shown to have strong antimicrobial activities; it could be used as a potential food additive for improving the quality of various milk-based products due to its various bioactive properties. Future studies should be conducted for manufacturing functional dairy products with the addition of peppermint oil to prevent and/or alleviate specific diseases.

• Journal of Dairy Science and Biotechnology
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• 제36권3호
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• pp.155-163
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• 2018

In this study, the antibacterial activity of Aronia melanocarpa (black chokeberry) ethanol extract against Bacillus cereus, Staphylococcus aureus, Cronobacter sakazakii, and Salmonella Enteritidis was investigated using the spot-on-lawn assay. The results showed that this extract exhibited antibacterial activities against Bacillus cereus (complete inhibition) and Staphylococcus aureus (partial inhibition), but did not inhibit the growth of Cronobacter sakazakii and Salmonella Enteritidis. This study shows that the Aronia melanocarpa (black chokeberry) ethanol extract was more effective against Gram-positive bacteria than Gram-negative bacteria. Hence, it is suggested that Aronia melanocarpa could be a useful food supplement, and could be utilized as a naturally derived additive for maintaining the safety of various dairy products. Furthermore, future research should be conducted to examine the possibility of using such products as functional ingredients for improving the anti-oxidative and anti-inflammatory activities of food products.

• 한국임상수의학회지
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• 제9권1호
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• pp.273-276
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• 1992

Present study was undertaken in order to evaluate the lead contents of various feedstuffs produced in Chonbuk area. Fifty-five samples were collected from 5 regions. All of the samples were dry-ashed and analyzed for lead content using atomic absorption spectrophotometer. The results obtained were summarized as followed : 1. The lead content in $\mu\textrm{g}$/g of agriculture by-products were measured with the following mean values and ranges : soybean pod 7.01${\pm}$2.49(4.71∼10.66), rice bran 4.56${\pm}$0.90(3.17∼5.65), rice straw 3.89${\pm}$2.01(1.29∼6.33), rice hull 1.92${\pm}$1.77(0∼3.63). 2. The lead content in $\mu\textrm{g}$/g of pasture plants were measured with the following mean values and ranges : kudzu 20.29${\pm}$4.75(14.84∼25.34), clover 13.92${\pm}$2.68(10.17∼16.44), lespedeza 10.91${\pm}$9.00 (3.35∼23.54), sagebrush 10.75${\pm}$3.28(5.14∼13.18), Korean lawn grass 5.05${\pm}$2.29(2.57∼7.79), cornstalk 3.91${\pm}$2.55(0.50∼7.22), reed 2.92${\pm}$1.88(0.50∼5.73). 3. Seventeen samples (30.9%) out of 55 samples showed lead content of more than 10$\mu\textrm{g}$/g which are said to be the upper limit value for all animals. 4. The regional mean v짓ues for 리1 kinds of samples were Wanju-gun 10.02${\pm}$8.55$\mu\textrm{g}$/g, Mooju-gun 8.16${\pm}$4.91$\mu\textrm{g}$/g, Namwon-gun 7.09${\pm}$4.59$\mu\textrm{g}$/g, Okgu-gun 7.06${\pm}$7.11$\mu\textrm{g}$/g, Jeongup-gun 6.35${\pm}$6.50$\mu\textrm{g}$/g.

• 한국임상수의학회지
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• 제9권1호
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• pp.267-271
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• 1992

Present study was undertaken in order to evaluate the cadmium contents of various feedstuffs produced in Chonbuk area. Fifty-five samples were collected from 5 regions. All of the samples were dry-ashed and analyzed for cadmium content using atomic absorption spectrophotometer. The results obtained were summarized as followed : 1. The cadmium content in $\mu\textrm{g}$/g of agriculture by-products were measured with the following mean values and ranges : soybean pod 3.15${\pm}$0.51(2.40∼3.81), rice bran 2.87${\pm}$0.23(2.50∼3.09), rice straw 1.66${\pm}$0.85(1.08∼3.14), rice hull 0.86${\pm}$0.45(0.41∼1.35). 2. The cadmium content in $\mu\textrm{g}$/g of pasture plants were measured with the following mean values and ranges : kudzu 5.65${\pm}$1.00(4.87∼7.29), sagebrush 4.86${\pm}$1.24(2.89∼6.24), clove, 4.28${\pm}$0.75(2.99∼4.86), lespedeza 3.99${\pm}$1.21(2.38∼4.94), Korean lawn grass 2.24${\pm}$1.04(1.33∼3.44), reed 1.99${\pm}$0.95(0.88∼2.91), cornstalk 1.61${\pm}$0.99(0.38∼3.12). 3. The regional mean values for all kinds of samples were Okgu-gun 3.19${\pm}$2.09$\mu\textrm{g}$/g, Mooju-gun 3.15${\pm}$1.73$\mu\textrm{g}$/g, Wanju-gun 3.05${\pm}$1.76$\mu\textrm{g}$/g, Namwon-gun 2.85${\pm}$1.70$\mu\textrm{g}$/g, Jeongup-gun 2.85${\pm}$1.25$\mu\textrm{g}$/g.

• 한국잡초학회지
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• 제31권2호
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• pp.199-204
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• 2011

본 연구는 예초 직후에 prohexadione-calcium을 처리하여 잔디의 생장 상태를 조사하였다. 한국잔디로 조성된 잔디밭이나 골프장을 효과적으로 관리하기 위하여 한국잔디의 예초 직후 prohexadionecalcium의 농도를 달리하여 엽면 처리한 결과 무처리와 비교하여 잎의 생장이 현저히 감소되는 것으로 나타났으며 prohexadione-calcium의 1회 처리로도 그 효과가 처리후 50일까지 잎의 생장이 46~50%정도 감소되는 것으로 나타났고, 엽폭도 처리농도가 높을수록 무처리에 비하여 좁아졌다. Prohexadionecalcium 처리후 건물중의 변화를 보면 처리후 2주후 부터 무처리에 비하여 현저하게 감소하는 것으로 나타났는데, 처리 농도가 높을수록 건물중이 작았으며 처리 40일까지도 건물중의 증가가 무처리에 비하여 유의하게 감소하는 것으로 나타났다. 또한 엽색도는 prohexadione-calcium의 처리구가 무처리구에 비하여 높았다.

• Journal of Microbiology and Biotechnology
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• 제24권6호
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• pp.740-747
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• 2014

Bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae (Xoo) is a very serious disease in rice-growing regions of the world. In spite of their economic importance, there are no effective ways of protecting rice plants from this disease. Bacteriophages infecting Xoo affect the population dynamics of the pathogen and consequently the occurrence of the disease. In this study, we investigated the diversity, host range, and infectivity of Xoo phages, and their use as a bicontrol agent on BLB was tested. Among the 34 phages that were isolated from floodwater in paddy fields, 29 belonged to the Myoviridae family, which suggests that the dominant phage in the ecosystem was Myoviridae. The isolated phages were classified into two groups based on plaque size produced on the lawn of Xoo. In general, there was a negative relationship between plaque size and host range, and interestingly the phages having a narrow host range had low efficiency of infectivity. The deduced protein sequence analysis of htf genes indicated that the gene was not a determinant of host specificity. Although the difference in host range and infectivity depending on morphotype needs to be addressed, the results revealed deeper understanding of the interaction between the phages and Xoo strains in floodwater and damp soil environments. The phage mixtures reduced the occurrence of BLB when they were treated with skim milk. The results indicate that the Xoo phages could be used as an alternative control method to increase the control efficacy and reduce the use of agrochemicals.

• 한국조경학회지
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• 제33권5호통권112호
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• pp.15-30
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• 2005

A deserted town once vibrant with active commercial activities around a railroad station now tries to find a way to escape from depression and revive its life with a renewed civic pride. An open space adjacent to the Main Street, the commercial district of Buzzards Bay, Massachusetts, is waiting to be transformed and reconfigured to be a new ecological park to boost the economy of the community. Bridge Park is 26-acre land abutting the Cape Cod Canal with a railroad bridge as a backdrop. The existing condition of the site with a small salt marsh, woodland, lawn, and the vestige of old railroad easement along with the proximity to the commercial district poses an interesting question of how to make a medium scaled ecological park within an urban context. This paper examines the winning design proposals for the Bridge Park submitted to the International Design Competition held in April, 2005. Six winning proposals were introduced and discussed in terms of categories related to the trend of contemporary landscape design such as; 1) ecological ordinariness and geometric figures, 2) topography and spatial imagination, 3) minimal programs and open put 4) time and process oriented design, 5) park and economic effects and 6) diagrammatic plan and photo montage. Bridge Park Design Competition confirms the complex characteristics representing the contemporary landscape design overcoming the dichotomy between nature and culture and the 'pastoral ecological design' and 'landscape as an art'. The Park becomes the activating agent for the community rejecting the conventional and passive role as a romantic picturesque landscape. Bridge Park International Design Competition is a meaningful event to test the idea of new ecological urban park, and to fine-tune the trend of the contemporary urban park design.

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2008년도 학술발표회 논문집
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• pp.1639-1643
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• 2008

국내에서의 범람홍수 및 범람으로 인한 제방붕괴 특성은 그 해석상의 어려움으로 인해 주로 과거 범람상황 및 지형도를 통한 경험적 추정 등에 의존해 오고 있는 실정으로 정교한 물리학적 이론에 근거하지 못하였으므로 범람홍수량, 제방의 붕괴폭 등을 정확하게 예측하기에는 미흡한 점이 많았다. 도시하천과 같이 제방 인접한 곳에 주거, 산업시설 등이 밀집하고 있는 지역에서의 제방붕괴로 인한 홍수류의 범람은 막대한 인명 및 재산피해를 발생시키는 큰 재해가 될 수 있다. 본 연구를 통해서 홍수시 제방월류로 인한 제방붕괴 양상을 예측하고 이를 통해 범람유량의 정확한 예측을 통하여 도시하천 제방 계획시 홍수피해와 투자액을 감안한 경제적인 하천 제방단면 결정을 위한 기본자료로 활용될 수 있고, 도시 중요시설물의 입지 선정시 홍수피해에 대한 판단자료로 이용될 수 있을 것이다. 본 연구에서 개발된 모형을 이용하여 Teton 댐, Mantaro landslide 댐, Spirit Lake 장애물 댐 등에 적용하여 개발된 모형의 정확성을 입증하였다. 적용결과 개발된 모형은 기존의 모형과 같은 결과를 나타내어 국내 계산환경에 적용가능함을 보여 앞으로 이용가능성이 큰것을 알 수 있었다. 적용결과 파이핑의 발생위치의 영향은 대체로 파이핑의 위치가 제체의 윗부분에 위치할수록 최대 유출량은 커지는 것으로 나타났으나 Lawn Lake 댐의 경우에는 사면의 식생의 영향으로 다른 결과를 나타내었다. 점착력은 경우에는 점착력이 클수록 유출량의 크기는 감소하는 것으로 나타났으며 마찰각은 최대 붕괴폭의 크기 및 발생시간, 댐의 높이와 폭, 저수용량 등과 유출량이 연관있어 좀 더 많은 계산결과를 이용하여 심도있는 고찰이 요구되었다. 입자의 중간크기, 공극률, 균일성의 경우 적용된 댐들 모두 유사한 결과를 나타내었고 변수들이 커질수록 유출량도 증가하였다. 댐 사면 경사의 경우에는 상,하류 사면 모두 경사가 완만할수록 유출량이 감소하였다. 위의 결과를 통해 개발된 제방붕괴 해석모형은 좀 더 많은 연구와 적용을 통해 개선과 검증이 이루어진다면 국내 제방붕괴해석에 필요한 해석모형이 될 수 있다고 판단되었다.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2018년도 추계학술대회
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• pp.100-100
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• 2018

Zoysia japonica Steud.(Zj) is a typical warm-season Korean lawn grass, which is used in many places such as river banks, roadside and soccer fields in Korea. Recently, it has also been used in school yards and the Saemangeum reclaimed land to reduce water pollution. Although the cultivated area of turfgrass is steadily increasing worldwide, it grows fast requiring frequent mowing and is difficult to grow in shady areas and the cold region. Therefore this study aims searching for useful gene(s) to develop abiotic stress tolerant and dwarf zoysiagrass. We isolated Ycf4 gene based on the sequence from Oryza sativa Japonica through RT-PCR and RACE PCR. Ultimately, open reading frame (ORF) of ZjYcf4 was 558bp long, encoding a protein of 186 amino acid residues. NCBI blast results showed that the ZjYcf4 protein is evolutionarily closely related to Ycf4 protein from Zoysia macrantha and Setaria italica (100% and 98%, respectively). To determine whether ZjYcf4 was involved in environmental stress in wild-type zoysiagrass, expression patterns of the gene were analyzed by real-time PCR under salt, cold and dark conditions. They were analyzed after each stress treatment for 3 hours. In salt and cold stresses, the expression was higher compared to control (3-fold and 1.5-fold, respectively), although there was a 1.6-fold decrease in expression under dark stress treatment. As reported previously, we suggest that ZjYcf4 gene affects abiotic stress such as salt, cold and dark.