• Journal of The Korean Society of Grassland and Forage Science
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• v.38 no.4
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• pp.260-265
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• 2018

Limited researches are available to demonstrate the effect of TMR (Total Mixed Ration) and SF (Separate feeding) system on ruminal methane production. Two experiments were performed at two different levels of daily feed intake (1.8 and 2.4% of BW) to investigate methane production by using a triplicated $2{\times}2$ Latin square design. Each experiment was conducted using six Holstein steers. Animals were provided either TMR or SF containing 73% concentrate and 27% forage, with the same ration of same ingredients. No differences in daily methane productions (MP) and per dry matter intake (DMI) were observed in both experiments. Relationship for daily methane production on DMI was $MP(g/d)=11.5({\pm}1.3){\times}DMI(kg/d)+14.2$, $R_2=0.73$, p<0.001.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.12
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• pp.814-824
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• 2018

This study was conducted to investigate VFA, monosaccharides and metabolites in rumen fluid according to feeding methods. Three castrated Hanwoo steers were used to the $3{\times}3$ Latin square design, 10 day for the diet adaptation period. VFA and monosaccharides which were not detected by HPLC and HPAEC however, those were detected by $^1H-NMR$. Among the metabolites measured by $^1H-NMR$ carbohydrate metabolites, pyruvate was detected only in the rumen fluid before feeding and succinate was detected before and after feeding rumen fluid. In amino acid total 9 metabolites were detected. In lipid metabolites, ethylene glycol was significantly higher (P<0.05) in before feeding Con group. In aliphatic acylic metabolite, trimethylamine N-oxide was no significant difference observed compare to Con group. In this study, many metabolites were observed in the rumen fluid by $^1H-NMR$, and it confirmed that rumen metabolic products were changed by feeding methods.

• ANNALS OF ANIMAL RESOURCE SCIENCES
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• v.28 no.2
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• pp.56-63
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• 2017

This study was conducted to determine the effects of supplementary levels of dietary betaine and energy on nutrient digestibility and physiological responses in growing pigs exposed to heat stress. A total of 12 barrows ($L{\times}Y{\times}D$; initial BW, $67.1{\pm}0.98kg$) were allocated into individual metabolic crates. Experimental design was a $4{\times}4$ Latin square with dietary energy and betaine levels (3,300 and 3,400 kcal/kg; 0 and 0.5%, respectively) and the experimental period (n=4). Feeding trial was performed during summer season (July and August). Digestibility of crude fat was higher (p<0.01) in the ME 3,400 kcal/kg than the ME 3,300 kcal/kg, but did not differ by betaine (p>0.05). Blood corpuscles and biochemical components were not influenced by dietary energy and betaine levels (p>0.05). Serum IgG was greater (p<0.05) in the ME 3,400 kcal/kg than the ME 3,300 kcal/kg, but cortisol was not differ among the treatment groups (p>0.05). In conclusion, our results showed positive effects by energy levels rather than betaine. Thus, further study need investigation of effects of high energy level in the pigs exposed to heat stress.

• Animal Bioscience
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• v.36 no.10
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• pp.1536-1545
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• 2023

Objective: The objective was to evaluate the effects of UV-B irradiated vitamin D-enriched yeast supplementation on milk yield, milk composition, vitamin D in milk, milk fatty acids, blood chemistry, and 25(OH)D status in dairy cows. Methods: Six Thai Friesian cows (milk production, 11.2±2.0 kg/d; body weight, 415.0±20.0 kg; and days in milk, 90.0±6.0) were allocated to each treatment in a 3×3 Latin square design, with three treatments and three periods. Each period of the Latin square lasted 49 days consisting of 14 days for diet adaptation and 35 days for sample collection. Dairy cows were randomly assigned to one of three treatments: i) feeding a basal diet without yeast (CON); ii) basal diet + 5 g of live yeast (75 IU/head/d of vitamin D₂; LY); and iii) basal diet + 5 g of UV-B irradiated vitamin D enriched yeast (150,000 IU/head/d of vitamin D₂; VDY). Feed intake and milk production were recorded daily, milk sample collection occurred on days 14 and 35 of each collection period, and blood plasma was collected on days 0, 7, 14, 21, 28, and 35 of each collection period. Results: The results show that after a trial period of 14 and 35 days, the VDY group had significantly higher vitamin D content in milk than the LY and CON groups (376.41 vs 305.15, 302.14 ng/L and 413.46 vs 306.76, 301.12 ng/L, respectively). At days 7, 14, 21, 28, and 35 of the experiment, cows fed the VDY group had significantly higher 25(OH)D₂ status in blood than the CON and LY groups (51.07 vs 47.16, 48.05 ng/mL; 54.96 vs 45.43, 46.91 ng/mL; 56.16 vs 46.87, 47.16 ng/mL; 60.67 vs 44.39, 46.17 ng/mL and 63.91 vs 45.88, 46.88 ng/mL), respectively. Conclusion: In conclusion, UV-B irradiated vitamin D-enriched yeast supplementation could improve vitamin D content in the milk and 25(OH)D status in dairy cows during the lactation period.

• Asian-Australasian Journal of Animal Sciences
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• v.24 no.10
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• pp.1393-1398
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• 2011

Four 4-month old Charolais${\times}$Dorset male sheep (initial liveweight $25.0{\pm}1.1\;kg$), fitted with rumen and abomasal fistulas and nourished by total intragastric infusions, were used to study the relationships between methionine (Met) supply, nitrogen (N) retention and plasma insulin-like growth factor-I (IGF-I). Four graded levels of Met, i.e. 0 g/16 g N, 1.76 g/16 g N, 3.52 g/16 g N and 7.04 g/16 g N, were infused into abomasums as experimental treatments. The sheep and treatments were allocated in a $4{\times}3$ incomplete Latin square design (Yudon square design). The experiment lasted 3 periods and each period was 10 days. Quadratic correlations were found between Met level (x, g/16 g N) and N retention (y, g/d): y = $-0.03x^2$+0.41x+2.62, $r^2$ = 0.66, n = 12, p = 0.008, and between methionine level (x, g/16 g N) and plasma IGF-I concentration (y, ng/ml): y = $0.80x^2$-4.53x+190.24, $r^2$ = 0.51, n = 12, p = 0.009. No significant correlation was found between plasma IGF-I (x, ng/ml) and N retention (y, g/d) (p>0.05). It was concluded that Met level had a significant influence on N retention and plasma IGF-I concentration whereas IGF-I might not be an important mediator in the regulation of N metabolism by Met in growing sheep nourished by total intragastric infusions.

• Asian-Australasian Journal of Animal Sciences
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• v.16 no.2
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• pp.204-210
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• 2003

To see the effect of Beragfat T-300, a by pass fat, on the production and composition of milk, four primiparous crossbred cows in their early lactation were used in a $4{\times}4$ Latin Square Design. Each period was of 30 days including 15 days of adjustment period. The diets were formulated to contain 0, 2.5, 3.5 and 4.5% of Bergafat and were isonitrogenous and isoenergetic. The intake of DM, OM, CP, NDF, ADF, Cellulose and ADL were not affected, however, the EE intake was increased by the supplementation of Bergafat in the diet of cows. The digestibilities of NDF and EE remained unaffected, whereas the digestibilites of DM, OM and CP were reduced. Milk yield remained unaltered, while 4%FCM yield increased as a result of adding Bergafat in the daily ration. Bergafat upto 4.5% of the diet DM can be added in the diet of crossbred cows without any adverse effect on the DM intake and digestibilities of DM and NDF. Furthermore, Bergafat does not cause any butter fat depression in the milk of cows.

• Asian-Australasian Journal of Animal Sciences
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• v.5 no.4
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• pp.741-746
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• 1992

The effects of 5%, 10%, 15% and 20% dietary cellulose levels on the nutrient utilization in chickens were investigated. Four experimental diets were alternatively given to 8 colostomized White Leghorn cockerels to make a $4{\times}4$ Latin-square design. The diets of 70 g.day were force-fed once a day, and water was given freely. The digestibilities of DM and energy increased linearly with the increase in dietary cellulose level. The digestibilities of ether extract and nitrogen-free extract were not so much influenced by the dietary cellulose level. The digestibility of acid detergent fiber was very low and not influenced by the dietary cellulose level. The digestibility of neutral detergent fiber was not different among the diets containing 5%-15% cellulose, but that of the 20% cellulose diet was diminished. This might be due to the reduction of hemicellulose digestibility. True digestibility and biological value of protein were also not influenced by the dietary cellulose level from 5% to 20%. In conclusion, no ill-effect was found even when the chicken was fed a diet containing 20% of cellulose.

• Asian-Australasian Journal of Animal Sciences
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• v.5 no.4
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• pp.695-698
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• 1992

Three Japanese Corriedale wethers were used in a $3{\times}3$ latin square design to determine the effect of soybean meal (SBM) supplementation on the eating and rumination behavior in sheep fed rice straw as a basal diet. Soybean meal was supplemented at three levels of 0 (control), 75 g and 150 g/day. Soybean meal supplementation had no significant effect on the daily time spent eating and rumination, whereas the rate of eating of rice straw was significantly faster (p<0.05) in sheep fed SBM-supplemented diets than in sheep fed control diet. However, when expressed per 100 g of neutral detergent fibre (NDF) intake, daily rumination time of sheep fed 75 and 150 g of SBM-supplemented diets was greatly reduced (p<0.01). The length of each rumination period, daily number of rumination periods and number of boli regurgitated were about constant for all SBM levels. Cyclic rate (rumination time per daily number of boli regurgitated) and rumination index (rumination time per 100 g of dry matter eaten) were significantly decreased (p<0.05) by SBM supplementation. The number of chews per bolus was not affected, whereas the bolus time reduced (p<0.05) and the rate of chewing during rumination was increased (p<0.05) by SBM supplementation.

• Asian-Australasian Journal of Animal Sciences
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• v.5 no.4
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• pp.687-693
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• 1992

The study was conducted to investigate the effect of soybean meal (SBM) supplementation on the voluntary intake of rice straw and ruminal fermentation characteristics. Balance trials were conducted with three Hapanese Corriedale wethers fed a rice straw alone (control), rice straw supplemented with 75 and 150 g of SBM/day in a $3{\times}3$ latin square design. Voluntary intake of rice straw in sheep fed both levels of SBM supplemented diets was significantly higher (p<0.05) than that in sheep fed control diet. Crude protein digestibility was significantly increased (p<0.05), but organic matter, crude fibre, neutral detergent fibre and acid detergent fibre digestibilities were not affected by SBM supplementation. Nitrogen balance was positive in sheep on both levels of SBM supplemented diets, but negative in animals on the control diet. Rumen ammonia and blood urea-nitrogen concentrations increased (p<0.05) as increasing level of SBM. Total volatile fatty acids, acetate, propionate, butyrate and valerate concentrations in rumen fluid were also significantly increased (p<0.01), but ruminal pH was decreased (p<0.05) by SBM supplementation.

• 한국생물공학회:학술대회논문집
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• 2000.04a
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• pp.239-242
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• 2000

Experimental studies on the effects of impeller to provide the microbial transglutaminase derived from Streptoverticillium mobaraense have been conducted. The optimal production medium was determined by latin-square design, and the effects of aeration and agitation were observed by using different sizes and shapes of impellers for the poduction of transglutaminase. The effects of pH and temperature were also evaluated for the production of transglutaminase in flasks. As a result, pH is more effective than temperature for both enzyme production and growth of the microorganism. The peak enzyme activity for transglutaminase in fermenter was 0.7 U/mL, but this was still well below the avereage enzyme activity, 1,3 U/mL, obtained in flask runs.