• Title/Summary/Keyword: Lateral root

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Growth and Chlorophyiil Biosynthesis of Vigna angularis under Lead Stress

  • Koo Suh-Young;Jin Sun-Young;Hong Jung-Hee
    • Environmental Sciences Bulletin of The Korean Environmental Sciences Society
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    • v.1 no.2
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    • pp.145-155
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    • 1997
  • The effect of various supplies of lead singly and in combination with aluminium on growth and chlorophyll biosynthesis was investigated in 7-day-old Vigna angularis seedlings. Expose to 50 uM Pb or more drastically reduced root elongation rate. Significant depressions in root growth was observed within 1 day and no recovery of growth was seen over the duration of treatment period. Root elongation decreased depending on the Pb concentrations. Root growth inhibition was stronger than shoot growth inhibition. The initiation of lateral roots appeared to be more sensitive to Pb than the growth of main roots. Inhibition of root and shoot elongation by Pb was lessened by combined exposure of Pb and Al, suggesting that the presence of Al reverse the inhibitory effect of Pb alone. With the histochemical sodium rhodizonate method the rate of Pb uptake was dependent on the Pb concentration and exposure time of the roots to Pb salts. Pb was first deposited on the root surface and then translocated radially in the root cap cells. During a longer Pb administration (up to 72 h) Pb penetration was nonuniform, with accumulation within the cortex or endodermis. There was drastic reduction in chlorophyll content by Pb. The Pb inhibition of chlorophyll synthesis was concentration dependent. $\delta-Aminolevulinic$ acid dehydratase (ALAD) activity exhibited distinct inhibition from control. Reduction in chlorophyll content was accompanied by proportional changes in ALAD activity. Chlorophyll content and ALAD activity were less affected by combined exposure of Pb and Al, suggesting that Al has a protective effect against the inhibiting action of Pb on photosynthetic activity.

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Chemical Components of Rusty Root of Ginseng (적변인삼의 화학적 성분에 관한 연구)

  • 이태수;목성균
    • Journal of Ginseng Research
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    • v.19 no.1
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    • pp.77-83
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    • 1995
  • This study was conducted to investigate the chemical components of rusty root for seeking the cause of rust. Na, Fe contents were higher in various tissues of six-year-old rusty root than healthy root. Contents of total sugar in epidermis of rusty root was low but crude lignin and suborn contents were high. Crude lipid content increased with the rate of rusty symptoms in each part of root respectively. Pentadecanoic, stearic, oleic, lignoseric acid contents increased in cortex of tap root with the rate of rusty symptoms but decreased in pith. Total ginsenoside and crude saponin contents increased , in pith and cortex of tap root with the rate of rusty symptoms but decreased in lateral and fine root.

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Studies on the Growth of Excised Root between Indica- Japonica Hybrids and Japonica Type in Paddy Rice (통일형수도와 일본형수도에 있어서 종근.분리근의 생육에 관한 연구)

  • 김진호;서인석;이성춘
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.34 no.2
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    • pp.147-154
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    • 1989
  • The experiments were carried out to compare the growth characteristics of excised seminal roots in Japonica type, Indica- Japonica hybrids and its parents. Based on the modified culture medium by Kawata et al., several levels of pH. sucrose and casamino acid were varied in the culture media. The excised root length at 27$^{\circ}C$ incubator was little different between Japonica type and Indica-Japonica hybrids, but at 30$^{\circ}C$ incubator the excised root elongation of Indica-Japonica hybrids was much better than that of Japonica type. The dry matter weight of excised root in Indica- Japonica hybrids was significantly heavier than in Japonica type at both 27 and 30$^{\circ}C$ incubators. The difference of dry root weight between Japonica type and Indica- Japonica hybrids was caused from the number of lateral roots, especially the root numbers of 25 cm length. The main and lateral seminal root growth of Japonica type grew best at the lot of 0.2 percent casamino acid. However, the lots of 0 and 0.2 percent casamino acid showed good results in the main and lateral excised root growth. respectively of Indica-Japonica hydrids. The growth of excised roots was better in Indica-Japonica hybrids than in Japonica type at even various levels of pH, sucrose and casamino acid. Between the parents of Indica - Japonica hybrids, the excised root growth of Indica type was more predominant than that of Japonica type, and the effect of casamino acid on the excised root growth in the formar was similar to that of Indica type. This result indicated that the growth characteristics of excised seminal roots in Indica Japonica hybrids were derived from the parent, Indica type.

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Anthraquinone Productivity by the Cultures of Adventitious Roots and Hairy Roots from Curled Dock (Rumex crispus) (소리쟁이 (Rumex crispus)의 부정근과 모상근 배양에 의한 Anthraquinone 생산성)

  • 장석원;김인현;한태진
    • Korean Journal of Plant Tissue Culture
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    • v.26 no.1
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    • pp.7-14
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    • 1999
  • In order to survey anthraquinone productivity from in vitro root culture, transformed hairy roots of Rumex crispus were induced from leaf segments by infection with Agrobacterium rhizogenes strain $A_4$ and compared with adventitious roots. The optimum condition of adventitious root formation from leaf segments was 5 $\mu$M NAA added to MS medium. Mannopine was detected in the extract of hairy roots by paper electrophoresis, but not in adventitious roots. Secondary root tips of both adventitious roots and hairy roots elongated without lateral root branching in hormone free MS medium, but primary root tips showed more rapid growth with extensive lateral root branching. MS basal medium was the best for growth of the adventious roots and hairy roots for anthraquinone content. Adventitious root tips and hairy root tips cultured in liquid MS medium supplemented with 0.05 $\mu$M NAA and 0.1 $\mu$M kinetin (contained 5% sucrose) showed the maximal growth and anthraquinone content. Anthraquinone content of hairy roots was increased by the culture periods, but was reduced after 25 days of culture.

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Assessment of endodontically treated teeth by using different radiographic methods: an ex vivo comparison between CBCT and other radiographic techniques

  • Demiralp, Kemal Ozgur;Kamburoglu, Kivanc;Gungor, Kahraman;Yuksel, Selcen;Demiralp, Gokcen;Ucok, Ozlem
    • Imaging Science in Dentistry
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    • v.42 no.3
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    • pp.129-137
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    • 2012
  • Purpose: To compare different radiographic methods for assessing endodontically treated teeth. Materials and Methods: Root canal treatments were applied in 120 extracted mandibular teeth, which were divided into four groups: (1) ideal root canal treatment (60 teeth), (2) insufficient lateral condensation (20 teeth), (3) root canals filled short of the apex (20 teeth), (4) overfilled root canal treatment (20 teeth). The teeth were imaged using intraoral film, panoramic film, digital intraoral systems (CCD and PSP), CCD obtained with portable X-ray source, digital panoramic, and CBCT images obtained at 0.3 $mm^3$ and 0.2 $mm^3$ voxel size. Images were evaluated separately by three observers, twice. Kappa coefficients were calculated. The percentage of correct readings obtained from each modality was calculated and compared using a t-test (p<0.05). Results: The intra-observer kappa for each observer ranged between 0.327 and 0.849. The inter-observer kappa for each observer for both readings ranged between 0.312 and 0.749. For the ideal root canal treatment group, CBCT with 0.2 $mm^3$ voxel images revealed the best results. For insufficient lateral condensation, the best readings were found with periapical film followed by CCD and PSP. The assessment of teeth with root canals filled short of the apex showed the highest percentage of correct readings by CBCT and CCD. For the overfilled canal treatment group, PSP images and conventional periapical film radiographs had the best scores. Conclusion: CBCT was found to be successful in the assessment of teeth with ideal root canal treatment and teeth with canals filled short of the apex.

A STUDY ON THE MICROLEAKAGE OF ROOT CANAL SEALERS (각종 근관충전용 시멘트의 미세누출에 관한 연구)

  • Cho, Ji-Sun;Hwang, Ho-Keel;Cho, Young-Gon
    • Restorative Dentistry and Endodontics
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    • v.21 no.1
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    • pp.202-217
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    • 1996
  • The purpose of this study was to compare the degree of microleakage of Glass-Ionomer root canal sealer possessed several enviable properties with that of the other sealers and to evaluate clinical performance. One hundred twenty single-rooted teeth were used in this experiment. The teeth were cleaned mechanically and immersed for 24 hours in 5.25% sodium hypochlorite and clinical crowns then were removed. After the root canals were instrumented using a step-back technique. one hundred twenty single-rooted teeth were divided into five groups of 24 in each. Group 1 : Tubli-Seal(Kerr Co., MI, U.S.A/ZOE-based), lateral condensation Group 2 : Sealapex(Kerr/Sybron, Romulus, MI/ $Ca(OH)_2$-based), lateral condensation Group 3 : AH 26(De Trey Co., Zurich Switzerland/Resin-based), lateral condensation Group 4 : Ketac-Endo(ESPE GMBH & CO. KG Seefeld:oberbay. Germany/ Glass Ionomer Cement-based), lateral condensation Control group : no sealer. lateral condensation And then. the root canals were obturated by lateral condensation technique with gutta-percha and experimental sealers. The control group were obturated without sealer. The teeth were placed in a vacuum chamber for 15 minutes and immersed 2% methylene blue under vacuum for 15 minutes. The teeth were passively stained for 1 week and 2 weeks and were cleared and evaluated for linear dye leakage using Tool maker's microscope(${\times}200$). The results were as follows: 1. There were statistically significant differences in the degree of dye penetration between the control group and experimental groups(p<0.05). 2. In the experimental groups, Sealapex($1.2871{\pm}0.9180mm$) exhibited the lowest mean value of dye penetration, followed by Ketac-Endo($1.4432{\pm}0.8082mm$), AH 26($1.5030{\pm}0.7752mm$) and Tubli-Seal($1.6458{\pm}1.0292mm$)(p>0.05). 3. There were statistically significant differences in the variation of microleakage between 1 week and 2weeks in Tubli-Seal and Seal apex groups (p<0.05). 4. The degree of dye penetration of all groups were increased as the time elapsed and AH 26 showed the lowest variation(+0.11) and Tubli-Seal(+ 1.03) showed the highest variation (p<0.001).

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Lateral Root Formation and Growth of Soybean Sporouts Treated with Various Solutions (다양한 물질처리에 의한 콩나물의 세근형성 및 생장)

  • Kang, Jin-Ho;Park, Cheol-Jong;Yoon, Soo-Young;Jeon, Seung-Ho;Hong, Dong-Oh
    • Korean Journal of Medicinal Crop Science
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    • v.13 no.1
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    • pp.6-10
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    • 2005
  • Lateral roots of soybean sprouts should deeply affect their quality and marketability. The study was done to compare the effects of ocher, chitosan, corn kennel, tea (Thea sinensis L.) and hard rubber tree (Eucommia ulmoides Oliver) leaf extracts on lateral root formation, growth and morphological characters of the sprouts. Seeds of three cultivars, Pungsannamulkong, Sowonkong and Junjery, were imbibed for 5 hours into their 10% solutions and cultured for 6 days. The cultured sprouts were classified into 4 categories to calculate their composition rates on the base of hypocotyl lengths;>7 cm (A), 4 to 7 cm (B), < 4 cm (C) and not germinated (D), and their morphological characters, fresh and dry weights were measured. Composition rate of A was the lowest in Junjery of the three cultivars, while that of C showed reverse result compared to A. This results was the most distinct in hard rubber tree leaf extracts (HRTLE) of the five treatments. In HRTLE treatment, lateral root formation rate were formed in almost of Sowonkong although reduced in order of Pungsannamulkong and Junjery. However, there was no significant difference between the other treatments. Lateral roots per sprout were the lowest in HRTLE treatment of the 5 treatments. In all treatments except the chitosan treatment, the roots were most formed in Sowonkong but least in Junjery. Sprout length adding hypocotyl and root was the shortest in Junjery compared to the other two cultivars. and was the longest in tea leaf extract treatment but the shortest in HRTLE treatment. The result in total fresh weight of sprouts was similar to that of the sprout length.

EFFECTIVENESS OF CUSTOMIZED MASTER CONE ON APICAL SEALING IN VARIOUS APICAL SIZE OF PREPARED ROOT CANALS (근단부 크기에 따른 customized master cone의 치근단 밀폐효과에 관한 연구)

  • Hong, Hye-Young;Choi, Ho-Young;Choi, Gi-Woon
    • Restorative Dentistry and Endodontics
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    • v.27 no.1
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    • pp.66-76
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    • 2002
  • The purpose of this study is to evaluate the effectiveness of customized master cone on apical sealing in various apical size of prepared root canals, that is MAF(Master Apical File) and to know at which apical size the apical leakage is to be significantly reduced using customized master cone. 120 extracted single rooted premolars were divided into four groups according to their apical size(MAF), #30, 40, 50 and 60. And then, each group was subdivided into three in accordance with three obturation methods, lateral condensation with standardized master cone, lateral condensation with chloroform-dipped customized master cone, and continuous wave of obturation technique. Resorcinol-formaldehyde resin was used for the microleakage test of this study. Teeth were sectioned horizontally at 1.5mm(Level 1), 2.5mm(Level 2), and 3.5mm(Level 3) from the anatomical root apex using low speed microtome. All sections were examined under $\times$40 magnification with a stereomicroscope, photographed, and then scanned. With the scanned images, resin-infiltrated area presenting the microleakage was calculated using SigmaScan/Image, and the ratio of leakage to the total root canal area of each group was analyzed statistically(one way ANOVA). The results were as follows ; 1. In groups of MAF #30, there was no significant difference of mean leakage ratio among three obturation methods at all three levels. 2. In groups of MAF #40, the group using lateral condensation with customized master cone had the low-est mean leakage ratio at all three levels, but there was no significant difference among three obturation techniques. 3. In groups of MAF #50, the mean leakage ratio of the group using lateral condensation with standard master cone was the highest among those of three obturation techniques at level 1, and this difference was statistically significant(p<0.05). 4 In groups of MAF #60, the groups using lateral condensation with standard master cone had also the highest mean leakage ratio at all levels, but there was no significant difference at level 1 and 2. At level 3, the leakage of the group using lateral condensation with standard master cone was significantly higher than that of the group using continuous wave of obturation(p<0.05). The results of this study suggested that the obturation method using customized master cone or the continuous wave of obturation is more effective for apical sealing than that using standardized master cone when MAF is larger than #50.

Absorption Characteristics of Puffed Red Ginseng Lateral Root, Red Ginseng Main Root and White Ginseng Lateral Root Powder (팽화 홍미삼, 홍삼절편 및 백미삼 분말의 흡습특성)

  • Kim, Sang-Tae;Youn, Kwang-Sup;Kwon, Joong-Ho;Moon, Kwang-Deog
    • Food Science and Preservation
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    • v.15 no.1
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    • pp.30-36
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    • 2008
  • In this study, raw ginseng produced by different method; was puffed and the absorption characteristics of the puffed ginseng powders were investigated Raw ginseng preparations, including white ginseng lateral root (WGL), red ginseng lateral root (RGL) and red ginseng main root (RGM) with 15 % moisture were puffed at a pressure of $7kg_f/cm^2$. The equilibrium moisture contents of puffed powders were affected by temperature and water activity. The monolayer moisture content determined by 1he BET equation was 0.034-0.045g $H_2O/g$ solid. The $R^2$ parameter of 1he BET equation was higher than that of the GAB equation. The absorption enthalpies, calculated using various water activities, showed a decreasing trend with increasing water activity. Amongst models applied for predicting equilibrium moisture content, 1he Kuhn model was 1he best fit for puffed ginseng powders, giving 1he lowest prediction deviation of 2.83-8.65% The prediction model equation for water activity included 1he variable of time, water activity (RH/l00) and temperature, whereas an equation featuring the parameters of time and water activity was the best model equation identified.

Effects of Cultural Soil Texture on Growth and Quality of Glycyrrhiza uralensis Fischer (감초 생육 및 품질에 미치는 재배 토성의 영향)

  • Nam, Sang Young;Kim, In Jae;Choi, Seong Yel;Kim, Min Ja;Kim, Young Ho;Song, In Gyu;Lee, Guang Jae;Park, Jae Ho;Kim, Tae Jung
    • Journal of the Korean Society of International Agriculture
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    • v.23 no.5
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    • pp.531-536
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    • 2011
  • This study was conducted to investigate the effects of cultural soil textures on growth and quality of Glycyrrhiza uralensis Fischer from 2009 to 2010. The obtained results from this study were summarized as follows; The growth of stem and leaf were superior to one year old G. uralensis, and surface runner and root growth tended to be better in 2 years old G. uralensis. The weight of stem and leaf were heavy in sandy loam, and plant height, branches, stem diameter in sandy clay loam were better than other soil texture. The growth characteristics, such as length, number and weight of surface runner, was better in order of sandy clay loam > sandy loam > loamy sand. The length of main and lateral root was longer in loamy sand soil than other treatments, and the diameter of main and lateral root was more thicker in sandy loam than others. The number of lateral root was higher in the sandy loam than other treatments. The yield of main and lateral root was in order of sandy loam > sandy clay loam > loamy sand soil. Marketable root yield of one year old and two year old G. uralensis were increased 57% and 71% in sandy loam compare to a loamy sand as 204 kg/10 a, respectively. The content of glycyrrhizinic acid was the hightest as 1.62% in sandy clay loam soil in one year old, and as 1.58% in sandy loam soil in two years old of G. uralensis, respectively.