• Molecules and Cells
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• 제21권1호
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• pp.141-146
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• 2006

We previously showed that NtCDPK1, a tobacco calcium-dependent protein kinase, interacts with and phosphorylates the Rpn3 regulatory subunit of the 26S proteasome, and that both NtCDPK1 and Rpn3 are mainly expressed in rapidly proliferating tissues, including shoot and root meristem. In this study, we examined NtCDPK1 expression in roots using GUS expression in transgenic Arabidopsis plants, and investigated its function in root development by generating transgenic tobacco plants carrying a sense NtCDPK1 transgene. GUS activity was first detected in roots two days after sowing. In later stages, strong GUS expression was detected in the root meristem and elongation zone, as well as the initiation sites and branch points of lateral roots. Transgenic tobacco plants in which NtCDPK1 expression was suppressed were smaller, and their root development was abnormal, with reduced lateral root formation and less elongation. These results suggest that NtCDPK1 plays a role in a signaling pathway regulating root development in tobacco.

• 대한치과의사협회지
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• 제13권5호
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• pp.415-416
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• 1975

1. The author have had a case of class V and class Ⅲ combined carious lesion extended on to root surface of upper right lateral incisor. 2. The patient was 40 years old female. 3. After routine root canal treatment of the tooth, the gingival flap was made by vertical incision on gingival tissue between distal surface of upper right lateral incisor and mesial surface of upper right canine. 4. Cavity preparation and amalgam filling on the carious lesion were performed and the flap was sutured.

• 한국자원식물학회지
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• 제26권4호
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• pp.503-510
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• 2013

본 연구는 졸참나무의 건전한 1-1 조림용 묘목을 생산하기 위해 초기 묘고가 다른 묘목에서 적절한 단근의 강도를 구명하기 위해 실시하였다. 이를 위해 1-0 노지묘의 크기를 구분하여 단근(5, 10, 15 cm)을 실시하였다. 1년의 생육기간이 지난 시점에서 묘고를 비롯한 지상부 생장량과 주근 및 측근의 발달 특성을 비교 분석하였다. 지상부의 생장은 초기 묘목 크기와 단근에 의해 다양한 차이가 나타났으며, 측근의 발달은 묘목 크기에 따른 영향이 큰 것으로 나타났다. 초기 묘고가 큰 묘목군에서는 15 cm로 단근된 묘목이 가장 우수한 지상부 생장량을 나타내었으며, 초기 묘고가 작은 묘목군에서는 15 cm와 10 cm로 단근된 묘목이 우수한 생장량을 나타내었다. 묘목 크기에 따른 생장은 초기 묘고가 큰 묘목이 작은 묘목보다 우수한 지상부 생장을 나타내었으며, 주근의 재생산량과 새롭게 발달된 측근의 수도 많았다. 하지만 측근의 길이 및 무게 생장은 작은 묘목이 더 많았다. 결론적으로 본 연구결과를 종합해 보면, 고품질의 묘목을 생산하기 위해서는 묘고가 큰 1-0묘를 생산하는 것이 다양한 장점을 가지며, 초기 묘고가 26 cm 이상의 큰 묘목은 15 cm로 단근하고, 초기 묘고가 24 cm 이하의 묘목은 10 cm로 단근하는 것이 가장 이상적인 단근길이라고 판단된다.

• Molecules and Cells
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• 제41권10호
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• pp.923-932
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• 2018

Ethylene regulates numerous aspects of plant growth and development. Multiple external and internal factors coordinate ethylene production in plant tissues. Transcriptional and post-translational regulations of ACC synthases (ACSs), which are key enzymes mediating a rate-limiting step in ethylene biosynthesis have been well characterized. However, the regulation and physiological roles of ACC oxidases (ACOs) that catalyze the final step of ethylene biosynthesis are largely unknown in Arabidopsis. Here, we show that Arabidopsis ACO1 exhibits a tissue-specific expression pattern that is regulated by multiple signals, and plays roles in the lateral root development in Arabidopsis. Histochemical analysis of the ACO1 promoter indicated that ACO1 expression was largely modulated by light and plant hormones in a tissue-specific manner. We demonstrated that point mutations in two E-box motifs on the ACO1 promoter reduce the light-regulated expression patterns of ACO1. The aco1-1 mutant showed reduced ethylene production in root tips compared to wild-type. In addition, aco1-1 displayed altered lateral root formation. Our results suggest that Arabidopsis ACO1 integrates various signals into the ethylene biosynthesis that is required for ACO1's intrinsic roles in root physiology.

• Imaging Science in Dentistry
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• 제47권3호
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• pp.181-187
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• 2017

Purpose: The purpose of this study was to measure the buccal bone thickness and angulation of the maxillary incisors and to analyze the correlation between these parameters and the root position in the alveolar bone using cone-beam computed tomography (CBCT). Materials and Methods: CBCT images of 398 maxillary central and lateral incisors from 199 patients were retrospectively reviewed. The root position in the alveolar bone was classified as buccal, middle, or palatal, and the buccal type was further classified into subtypes I, II, and III. In addition, the buccolingual inclination of the tooth and buccal bone thickness were evaluated. Results: A majority of the maxillary incisors were positioned more buccally within the alveolar bone, and only 2 lateral incisors(0.5%) were positioned more palatally. The angulation of buccal subtype III was the greatest and that of the middle type was the lowest. Most of the maxillary incisors exhibited a thin facial bone wall, and the lateral incisors had a significantly thinner buccal bone than the central incisors. The buccal bone of buccal subtypes II and III was significantly thinner than that of buccal subtype I. Conclusion: A majority of the maxillary incisor roots were positioned close to the buccal cortical plate and had a thin buccal bone wall. Significant relationships were observed between the root position in the alveolar bone, the angulation of the tooth in the alveolar bone, and buccal bone thickness. CBCT analyses of the buccal bone and sagittal root position are recommended for the selection of the appropriate treatment approach.

• Journal of Plant Biology
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• 제36권1호
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• pp.43-49
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• 1993

In order to survey possibility to produce saikosaponin from in vitro hairy root culture, culture of callus, adventitous root, and hairy root of Bupleurum falcatum L. were estabilished, and quantitative and qualitative aspects in saikosaponin extracted from these were compared with those of cultivated root. Callus grew well in MS medium containing 0.9 $\mu$M 2, 4-D. In contrast, both of adventitous root and hairy root grew well in hormone-free MS medium. However, hairy root showed more rapid growth with extensive lateral root branches, characteristics of lower content of water and softer than in adventitous root. Among the selected lines of adventitous root and hairy root were observed difference in the growth rate. Mannopine, one of opine synthesized in the transformed tissue with Agrobacterium rhizogenes. A4 were detected in the extract of hairy root lines. Pattern and content of crude saponin from adventitous and hairy root showed no difference, but somewhat difference from those of cultivated root. However, in callus, distinct production-aspect of saponin was not observed.

• Restorative Dentistry and Endodontics
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• 제15권2호
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• pp.104-114
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• 1990

The purpose of this study was to evaluate the adaptation of root canal filling material to the dentinal wall of root canal and to compare the sealing ability of the root canal filling materials using ultrasonic endodontic instrument with injection-molded thermoplasticized gutta-percha filling method and lateral condensation method. Fifty fresh human single root exlracted for orthodontic treatment, were randomly selected, and instrumented by step-back technique. And then, the teeth were divided into 5 groups according to each root canal filling methods. In the experimental group 1 and group 2, the root canals were filled with gutta perdia cases using ultrasonic instrument with and without sealer. In the experimental group 3 and 4, using jection-moldeed thermoplasticized gutta-percha method by obtul$^{(R)}$ canals were filled with and without sealer. In the control group, the canals were filled with sealer by lateral candensation. And then, 5 teeth of each group were immersed in black Indian ink, decalcified and cleared. The depth of dye penetration into the root canal were evaluated with stereoscope (Reichert Ltd., USA). Among the 5 teeth remaining in each group, the single longituding grooves were made on the labial and lingual root surfaces and then immersed in the liquid nitrogen to fracture the teeth spontaneously without any distortions of gutta-percha. Each specimens were examined with X-650 Scanning Electron Microscope(Hitachi ltd, Japan) to show the adaptation to the canal wall, void, homogenicity of filling material and location of gutta-percha or sealer in the dentinal tubules of the root canal. The observations were as follows : 1. The experimental group 1 showed smaller mean dye penetration than control group, and showed the penetraton of sealer in the dentinal tubules of apical third of the root canal. 2. The experimental group 2 and group 4 showed the penetration of gutta-percha in the dentinal tubules of root canals. 3. The experimental group 1 and group 3 showed less mean dye penetration than the experimental group 2 and group 4. 4. The experimental group 1 and group 2 showed better adaptation of filling materials than control group.

• Restorative Dentistry and Endodontics
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• 제7권1호
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• pp.25-31
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• 1981

One of the most important factors for successful endodontic therapy is an accurate length determination of physiological root apex. Some methods suggested for the measurement of root canal length, include digital-tactile sense and roentgenographic technique with measuring wire, scale and grid. But these methods do not derermine an accurate working length to physiological root apex. Recently electronic measuring devices are used to locate the physiological root apex in root canal length determination and these devices are accepted as an effective apparatus. The 89 patients (116 teeth, 144 canals) among the out-patients of Yonsei University Dental Infirmary, who had had an endodontic treatment in the Department of Operative Dentistry, were measured by the Root-Canal Meter$^{(R)}$ as an electronic device, and radiographs to determine the distribution and location of physiological root apex, then the following results were made: (1) Range of ${\pm}$1mm from the radiographic root apex were present in 88.88% (128 canals) of the subjects. (2) Physiological root apex and radiographic root apex were coincided in 31.94% (46 canals) of the subjects. (3) The actual length of the physiological root apex of the teeth were as follow; A : in the maxillary central incisor : 0.46mm B : in the maxillary lateral incisor : 0.44mm C : in the maxillary canine : 0.44mm D : in the maxillary 1st premolar : a) Buccal : 0.59mm b) Lingual : 0.34mm E : in the maxillary 2nd premolar : 0.54mm F : in the maxillary 1st molar : a) Mesio-buccal : 0.50mm b) Disto-buccal : 0.42mm c) Lingual : 0.56mm G : in the mandibular central incisor : 0.62mm H : in the mandibular lateral incisor : 0.45mm in the mandibular canine : 0.54mm J : in the mandibular 1st premolar : 0.47mm K : in the mandibular 2nd premolar : 0.34mm L : in the mandibular 1st molar : a) Mesio-buccal : 0.54mm b) Mesio-lingual : 0.31mm c) Distal : 0.37mm.

• 한국작물학회지
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• 제49권3호
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• pp.216-221
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• 2004

콩나물 재배에서 생장조절제 BA 흡수량은 처리방법에 따라 커다란 영향을 받는다. 본 연구는 BA 처리효과를 극대화하여 처리량을 줄일 수 있는 방법을 모색하고자 BA 처리 직후 첫 관수까지 경과시간이 콩나물의 생장과 세근발생에 미치는 영향을 조사하기 위하여 2 ppm BA용액에 5시간 침종한 풍산 나물콩, 소원콩 및 준저리의 종자를 재배시 첫 관수까지 20, 30 및 $40^{\circ}$로 aeration 온도를 달리하거나, $25^{\circ}$에서 1, 2, 3, 4시간으로 aeration기간을 달리하여 처리한 후에 6일간 재배한 결과를 요약하면 다음과 같다. 1, 침종과 첫 관수간 aeration온도 및 기간에 따라 각공시품종의 발아 및 생장 정도는 다른 것으로 조사되었다. 2. 콩나물의 발아 및 생장, 세근발생 개체비율, 개체당 세근수 및 생체중은 BA 용액에 침종한 후 첫 관수까지의 aeration온도의 영향은 미미한 반면, 공시품종의 영향을 크게 받는 것으로 조사되었다. 3. 세근발생 개체비율은 aeration기간을 4시간으로 길게 할수록 감소하였던 반면, 개체당 세근수는 3시간 이상의 aeration 기간간에는 차이가 없었다. 4. 하배축 및 뿌리의 길이는 1시간의 aeration을 가할 경우 가장 길었으나, 3시간 이상 aeration을 가할 경우 차이가 없었다. 5. 자엽, 하배측, 뿌리 및 전체 생체중도 1시간 aeration을 가할 경우 가장 많았으며, 3시간 이상 aeration을 가할 경우 차이가 없었다

• Journal of Ginseng Research
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• 제22권4호
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• pp.274-283
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• 1998

It has generally been accepted that quality of ginseng should be determined not by the content of a single component but by composition and balance of total active principles. However, there still can be an exception with a product in which a given ginsenoside is used for the treatment of a specific disease. Although ginsenosides have been regarded to be major active components of ginseng and employed as index components for the quality control, it does not consistent with the traditional concept on ginseng quality creterion; main root has been more highly appreciated than the lateral or fine root. Content of ginsenosides in the lateral or fine root is much higher than that in main root. However, the ratio of protopanaxadiol (PD) and protopanaxatriol (PT) saponins existing in various part of ginseng root is greatly different. The ratio of PD/PT saponins in main root is well balanced but the thinner the root is the higher the ratio. Thus far, a total of 34 different kinds of ginsenosides have been isolated from Korean (red) ginseng, and their pharmacological activities were elucidated partly. Interestingly, different ginsenoside shows similar or contrary effects to each other in biological systems, thus indicating the significance of absolute content of single ginsenoside as well as compositional patterns of each ginsenoside. Therefore, pharmacological activities of ginseng should be determined as a wholly concept. In these regards, standardization of ginseng material (fresh ginseng root) should be preceded to the standardization of ginseng products because ginsenoside content and non-saponin active principles such as polysaccharides and nitrogen (N)-containing compound including proteins are significantly different from part to part of the root. In other words, the main root contains less ginsenosides than other lateral or fine roots. Contents of polysaccharides and N-containing compound in main root is higher. However, the quality control of ginseng products focused on non-saponin compounds has limitation in applying to the analytical method, because of the difficult chemical analysis of these compounds. Content of ginsenosides, and ratios of PD/PT and ginsenoside Rb,/Rg, are inversely proportional to the diameter of ginseng root. Therefore, these can be served as the chemical parameters for the indirect method of evaluating from what part of the root does the material originate. Furthermore, contents of polysaccharides and N-containing compounds show inverse relationship to saponin content. Therefore, it seems that index for analytical chemistry of saponin can be applied to the indirect method of evaluating not only saponin but also non-saponin compounds of ginseng. From these viewpoints, it is strongly recommended that quality of ginseng or ginseng products be judged not only by the absolute content of given ginsenoside but also by varieties and compositional balance of ginsenosides, including contents of non-saponin active principles.