• 생물환경조절학회지
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• 제14권3호
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• pp.137-143
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• 2005

저장전 고온처리($38^{\circ}$, 48시간)는 참외의 당도, 산도, 비타민 C의 함량과 ${\alpha}$-tocopherol 활성을 높였으며, $3^{\circ}C$의 MA저장중 생체중 감소 이산화탄소, 에틸렌그리고 아세트알데히드 발생량을 낮게 유지시켰다. 또한 저장후 외관상 품질, 경도, 당도, 산도, 비타민 C, ${\alpha}$-tocopherol 활성 등 내적품질이 고온처리한 참외에서 높은 수준을 유지하였는데 특히 저온장해정도를 알 수 있는 이온용 출량이 고온처리구에서 낮아 수확후 고온처리로 저온장해가 완화되었음을 알 수 있었다. 고온처리를 하지 않은 대조구는 저장 25일부터 Alternaria rot이 발견되었으나 고온처리구는 저장 종료일인 39일까지 부패가 발견되지 않았으며 외관상 품질에 대한 저장일수로 세운 회귀식에 의하면 저장수명이 고온처리구에서 8일 이상 연장되었다. 참외의 경우 저장전 $38^{\circ}C$의 낮은 고온에서 장시간 열처리를 함으로써 살균효과 저온장해 완화효과와 더불어 수명연장까지 얻을 수 있었다.

• Journal of Periodontal and Implant Science
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• 제30권1호
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• pp.39-52
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• 2000

Polypeptide growth factor belong to a class of potent biologic mediator which regulate cell differentiation, proliferation, migration and metabolism. 1,25-dihydroxyvitamin $D_3$ decrease cell proliferation, and stimulate alkaline phosphatase activity which express in osteoblast during cell differentiation period. IGF-I is known to stimulate cell proliferation and differentiation too. 1,25-dihydroxyvitamin $D_3$ is known to increase IGF-I binding sites and IGF binding protein which inhibite the effect of IGF. The purpose of this study is to evaluate potential role of IGF-I as mediator that control the action of 1,25-dihydroxyvitamin $D_3$. MC3T3-E1 cell were seeded $5{\times}10^5/ml$ at 100mm culture plate in ${\alpha}-MEM$ containing 10% fetal bovine serum. After 48 hour incubation period, medium were changed ${\alpha}-MEM$ containing 5% fetal bovine serum. After 24 hours, $10^{-9}M$ 1,25-dihydroxyvitamin $D_3$ added. Total mRNA was extracted at 0, 6, 24, 48, 72 hour. PRPCR method was programed for the detection of IGF-I mRNA. In the both groups of 1,25-dihydroxy vitamin $D_3$ treated and control, alternative splicing form of IGF-I, IGF-IA and IGF-IB were expressed. In the 1,25-dihydroxyvitamin $D_3$ treated group, IGF-I mRNA expression was matained until 24 hour, there after expression was decresed. MC3T3-E1 cell were seeded $2.5{\times}10^4/ml$ at 24well plate in ${\alpha}-MEM$ containing 10% fetal bovine serum. After 48 hour incubation period, medium were changed ${\alpha}-MEM$ containing 3% fetal bovine serum. After 24 hours, $10^{-9}M$ 1,25-dihydroxyvitamin $D_3$ and 10 ng/ml IGF-I were added separately or together. Cell were cultured for 1 and 3 days, $2{\mu}Ci/ml\;[^3H]$ -thymidine was added for the last 24h of culture of each days. ${[^3H]}$-thymidine incorporation in to DNA was measured and expressed counter per minute(CPM). DNA synthetic activity was significantly decreased by 1,25-dihydroxyvitamin $D_3$ both at 1 day and 3 day, and in the combination group of 1,25-dihydroxyvitamin $D_3$ and IGF-I, DNA synthetic activity was also decreased both at 1 day and 3 days. IGF-I did not affect the DNA synthetic activity compared to control group both at 1 day and 3 day. From the above results, 1,25-dihydroxyvitamin $D_3$ was potent inhibitor of cell proliferaton in MC3T3-E1 cells. It assumed that the effect of 1,25-dihydroxyvitamin $D_3$ on osteoblast proliferation may be mediated in part by decreased level of IGF-I.

• 대한한방내과학회지
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• 제31권4호
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• pp.901-907
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• 2010

Hiccups are defined as abrupt involuntary contractions of diaphragm and intercostal muscles with sudden closure of the glottis. Hiccups are one of the common transient symptoms but when hiccups last more than 48 hours, or have no response to treatment, they are defined as intractable hiccups. Intractable hiccups can induce multiple problems such as weight loss, dehydration and sleep disturbance. We report a 76-year-old male patient who suffered from intractable hiccups for 6 days after laparoscopic cholecystectomy. Hiccupping was so severe as to continue all day long and limit sleeping and eating. We succeeded in cure intractable hiccups with acupuncture and herbal formula; Gyulyeohwadam-tang.

• 한국환경과학회지
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• 제5권6호
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• pp.719-725
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• 1996

To research the cause of the low and the poor visibility phenomena of Kimhae international airpot statistically, we analyzed the field routine data for the last five years from 1985 to 1989. The poor visibilities below 1km, 3km, and 5km usually occurred at about 6 o'clock in the morning under the condition of calm or light wind from south to southwest direction, and lasted for from 1 to 3 hours. they were caused by the radiative cooling and the inflow of moisture from the South Sea. The frequency of th low visibility(below 9km) recorded 48.1% a year. And the low visibility below 8km with relative humidity below 70% often occurred in the case of southeast, southwest, and northwest wind. And it reveals a peak at 11:00 a.m.. It is supposed to be caused by the pollutants flowing from the neighbouring industrila complex, Sasang, Jangnim and by the photochemical reaction. And, when the industrial direction from Kimhae international airport, the visibility and the air pollution may become worse and worse by the increased pollutants.

• Archives of Plastic Surgery
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• 제33권2호
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• pp.225-232
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• 2006

The aim of this study is to evaluate the clinical competence of medical student using short case test items. Development of short case test items with definition of an assessment of subject, choice of clinical stations sampling, identification of components of clinical competence to be evaluated, and assessment of practicability and results exploitation. For the last three years 28, 48 and 30 topics were developed annually. Among them 20, 23 and 23 topics were selected. About 50 students were examined annually. The mean score was 72.7, 57.1, 64.3 respectively. The mean preparation time of the students for the examination was 9.8 hours. About 60% of the students prepared the test in small group activity. Our experience suggests that short case test items be feasible for evaluation of student learning in Plastic Surgery. This method should be considered as a useful tool to assess medical student's competence or even an examination for board certification.

• 한국농공학회:학술대회논문집
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• 한국농공학회 2000년도 학술발표회 발표논문집
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• pp.266-271
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• 2000

This study was performed to find out how much water the cement hydration reaction need. It is real situation that it is difficult to find out the amount of chemical combined water with stoichiometric chemical reaction form. Because several variation occurred during hydration reaction it's not easy to divide water which used at cement paste mixture. In this study high temperature(105$^{\circ}C$) dry method was used to divide evaporable water and non-evaporable water. The last is combined water chemically and some free water absorbed to products of hydration physically. The test was processed with variation of water cement ratio from 10% to 45% with 5% intervals. The weight of cement paste specimens were measured after dry for 72hours at each checking time(0.5, 1, 3, 5, 10, 24, 48, 72, 168hour). In this study some conclusions such as follows were derived. Firstly, Pure combined water contents required at cement hydration result in 23.3percent of the weight of cement. Secondly, The sufficient mixing water needed to fully hydrated cement result in about 40∼45percent of weight of cement. That is, gel pores water could be about 16.7∼21.7percent of weight of cement.

• Computers and Concrete
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• 제8권4호
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• pp.389-399
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• 2011

The increasing concern for reinforced concrete structure durability has been justifying in many ways in the last few decades. However, there is no perfect durability test method till now. In this research an alternative method, which is based on the cumulative moisture loss percent of the concrete specimens after oven-drying, was proposed to estimate the durability performance of the concrete. Two temperatures were considered for the oven-drying tests: $100^{\circ}C$ and $200^{\circ}C$. Test results showed that oven-drying at $200^{\circ}C$ was obviously an unsuitable procedure to preserve the fragile microstructure of cement-based materials. By contrast, experimental results through oven-drying at $100^{\circ}C$ allowed estimating the moisture loss percent of cement-based materials in a more rational manner. Moreover, the magnitudes of the cumulative moisture loss percent obtained from oven-drying tests at $100^{\circ}C$ for 48 hours have good correlations with the data of other well-known methods, namely, electrical resistance test, water permeability test, and mercury intrusion porosimetry test. This investigation established that regarding the oven-drying test as one of the tests for evaluating the potential durability of concrete is considerably practicable.

• 사상체질의학회지
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• 제20권3호
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• pp.164-175
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• 2008

1. Objective The purpose of this study is to investigate the anti-aging and anti-oxidant effects of Bopyewon-tang(BPW) in Liver and Lung cell. 2. Methods This experiment was used the tissue of liver and lung cells of 6, 52 and 68 weeks old SD rats. Each age group was again divided into three groups. One group, as normal group, was not-treated cells, another group, as control group, was saline-treated cells, and the last group, as experimental group, was BPW -treated cells. After culture for 48 hours, each groups measured the level of SOD, GSH, MDA and NO in the tissue of liver and lung cells. 3. Results and Conclusion 1. The activity of SOD in liver of in 52 w-BPW group, 68 w-BPW group, and in lung of 68 w-BPW were significantly increased in comparison with those of the normal groups. 2. The levels of NO in liver of in 68 w-BPW group was significantly decreased in comparison with those of the normal groups. 3. The levels of MDA in lung of in 68 w-BPW group was significantly decreased in comparison with those of the normal groups.

• 한국수정란이식학회지
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• 제12권2호
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• pp.195-202
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• 1997

The present study was carried out to evaluate the effect of superovulation treatments on ovarian responses, oocyte recovery rates and grades of collected oocytes using an ultrasound-guided transvaginal approach in Korean native cows. Superovulation in cows was induced with two different regimenes: 1) FSH-decreasing dose(n=8): the cows were received twice per day for three days of the total dose of 400 mg of FSH-p, 2) FSH-single dose(n=9): the cows were administrated a single dose of 400 mg of FSH-p in 25% PVP. The Observation of visible follicles and collection of oocytes were performed 12 hours following the last FSH in FSH-decreasing dose group and 48 hours after the FSH-single dose injection. All visible follicles larger than 6 mm were punctured and aspirated with a 6.5 MHz convex-array ultrasound transducer designed for intravaginal use. The mean number of visible follicles(> 6 mm) was significantly(P<0.05) higher in the FSH-decreasing dose treatment (22.811.9) and FSH-single dose treatment (20.612.0) groups than the non-treatment group(7.0$\pm$8). The mean recovery rate of oocytes was not significantly(P<0.05) different between the treatment and control groups, but the mean number of collected oocytes was significantly(P<0.05) higher in the FSH-decreasing dose treatment( 12.611.5) and FSH-single dose treatment (11.813.6) groups than the non-treatment group(3.7$\pm$0.5). In conclusion, the FSH-single dose treatment at superovulation in cows for ultrasound-guided aspiration might increase the number of aspiratable follicles and the recovery rate of follicular oocytes as the FSH-decreasing dose treatment.

• 대한전기학회:학술대회논문집
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• 대한전기학회 1988년도 전기.전자공학 학술대회 논문집
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• pp.799-801
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• 1988

This paper was studied on the effects of Ca-La-Nb substitution and Zr/Ti ratio variation to Pb(Zr, Ti)$O_3$ system on structural, electrical, optical and sound level characteristics in order to develope the piezoelectric and electrooptic ceramic devices. Also the specimens were prepared by the two stage sintering technique. The molecular formular was X($CaO{\cdot}1/4La_{2}O_{3}{\cdot}1/4Nb_{2}O_{5}){\cdot}(1-X)Pb(Zr_{Y}Ti_{1-Y})O_{3}$(x=100X, y=100Y), and the variation of x was $6{\sim}12$, y was 60${\sim}$49 and second stage sintering time was 20${\sim}$40 hours. The experimental results obtained from this study are as follows : 1. The density was decreased, the grain size was increased according to increase of Ca-La-Nb substitution. 2. The crystal structure was rhombohedral in composition 6/60/40, and the crystal structure was tetragonal and cubic according to increase of Ca-La-Nb substitution. 3. The Ca substitution of PZT system enhanced the sintering property. The Pb site vacancy resulting from the substitution of La-Nb increased the dielectrical constant, the piezoelectric charge constant, the dielectric loss and decreased the coercive field. 4. The resistivity of PZT system which has the P type conduction mechanism increased according to substitution of La-Nb because of the substituent acting as donor. 5. The PZT ceramics varied from ferroelectric substance according to increase of Ca-La-Nb substituent. The coercive field and saturation remanent polarization decreased, and at last straight line according to increase of La-Nb substitution. 6. The amount of Ca-La-Nb substitution to improve the light transmittance of speciment was 10 mol%, the Zr/Ti ratio was 49/51, and the second stage sintering time was 40 hours. 7. According to Ca-La-Nb substitution, the specimens was to be transparent. The 7.5/51/49 specimen was suitable for transparent sound vibrator because it had 58% light transmittance (thick 0.2[mm], wave length 700[mm]) and 48% electromechanical coupling factor.