• 한국환경성돌연변이발암원학회지
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• 제22권4호
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• pp.236-242
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• 2002

Using agarose-coupled methotrexate, we have successfully isolated two proteins, which have strong interactions with methotrexate. The two proteins were analyzed by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry and identified as carbamoyl-phosphate synthetase 2 and phosphoribosylglycinamide formyltransferase, respectively. Interestingly, both of these two proteins are essential key enzymes in nucleotide biosynthetic pathways, like dihydrofolate reductase, a well-known methotrexate target. We confirmed the specificity of their interactions between methotrexate and two target proteins by the methods of competition binding assay, which were followed by western blotting using antibody against carbamoyl-phosphate synthetase 2 and phosphoribosylglycinamide formyltransferase, respectively. Moreover, we could observe that carbamoyl-phosphate synthetase 2 is overexpressed in methotrexate-resistant MOLT-3 cells comparing with control MOLT-3 cells. This result indicates that carbamoyl-phosphate synthetase 2 may be a novel target of methotrexate in cancer therapy. We propose that chemical proteomics can be a powerful technique to identify target proteins of a chemical.

• 동의생리병리학회지
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• 제32권5호
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• pp.315-320
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• 2018

The purpose of this study is to investigate reasearch trend of acupuncture treatment on shoulder impingement syndrome. This study investigated the recent studies about acupuncture treatment for shoulder impingement syndrome via searching Korean and foreign electronic databases(Research Information Services, National Digital Science Library, Oriental Medicine Advanced Searching Integated Services, PubMed, China National Knowledge Infrastructure). The search term was searched by combining 'shoulder impingement sydrome', 'subacrominal pain', 'acupuncture', 'needle therapy', and was limited to the articles published from 2005 to 2017. 14 studies were found to be analyzed according to the type of study, the number of cases, the type of treatment, the instruments for assessment and published year. 14 studies were published since 2005, 6 case reports, 8 randomized controlled trials. In the treatment of shoulder impingement syndrome, treatments such as acupuncture, acupotomy, pharmacopuncture, laser acupuncture, combined treatment of exercise and rehabilitation were performed. Visual analogue scale(VAS), range of motion(ROM) and various questionnaires were used as primary assessments. In each study, acupuncture treatment was reported to have a significant effect in the pain, range of motion and disability of shoulder impingement syndrome. In order to ensure objective evidence on acupuncture treatment, large scale case reports and randomized controlled trials should be continued.

• The Korean Journal of Physiology and Pharmacology
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• 제22권1호
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• pp.101-110
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• 2018

In this study, we aimed to investigate the neuroprotective effects of caffeic acid phenethyl ester (CAPE), an active component of propolis purified from honeybee hives, on photothrombotic cortical ischemic injury in mice. Permanent focal ischemia was achieved in the medial frontal and somatosensory cortices of anesthetized male C57BL/6 mice by irradiation of the skull with cold light laser in combination with systemic administration of rose bengal. The animals were treated with CAPE (0.5-5 mg/kg, i.p.) twice 1 and 6 h after ischemic insult. CAPE significantly reduced the infarct size as well as the expression of tumor necrosis $factor-{\alpha}$, hypoxiainducible $factor-1{\alpha}$ monocyte chemoattractant protein-1, $interleukin-1{\alpha}$, and indoleamine 2,3-dioxygenase in the cerebral cortex ipsilateral to the photothrombosis. Moreover, it induced an increase in heme oxygenase-1 immunoreactivity and interleukin-10 expression. These results suggest that CAPE exerts a remarkable neuroprotective effect on ischemic brain injury via its anti-inflammatory properties, thereby providing a benefit to the therapy of cerebral infarction.

• 한방안이비인후피부과학회지
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• 제22권2호
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• pp.192-200
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• 2009

Objective : The purpose of this study is to investigate the characteristics of patients with warts. Methods : We conducted statistics study on 100 patients, who had visited to Wart Clinic, from September 2008 to February 2009. Results : The results were as follows. 1. The wart was found more often from female(64%) than from male(36%). The age distributions of patients showed highest in 21-30 years, followed by 31-40 years, and 11-20 years. 2. The distributions of duration of warts showed highest in 1-5 years, followed by 6-10 years, and over 10 years. 3. The most common warts were Verruca plana(48%), Verruca vulgaris(25%), Verruca vulgaris with Palmoplantar wart(14%), Verruca plana with Verruca vulgaris(9%). 4. The Verruca vulgaris was found mostly in the hand, whereas the Verruca plana was seen mostly in the face. 5. Laser therapy was the most common previous treatment, followed by cryotherapy, immunotherapy in order. 6. The distributions of duration of treatment showed highest in 1-4 weeks, followed by 5-8 weeks, 9-12 weeks in order. 7. After treatment, 43 cases(43%) showed complete clearance, and 29 cases(29%) showed partial clearnace. Conclusion : We analyzed the characteristics of patients with warts. Further studies would be needed to develop the better treatment on warts.

• 한국자기학회지
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• 제21권1호
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• pp.32-36
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• 2011

DC 마그네트론 스퍼터링을 이용하여 제작한 고감도 거대자기저항 스핀밸브 박막 소자를 이용하여 수위 및 수온을 측정할 수 있는 센서를 제작하였다. 제작한 센서의 수위 및 수온 분해능은 각각 $400\;m{\Omega}/mm$와 $100\;m{\Omega}/^{\circ}C$ 이었으며, 이는 전립선 비대증 KTP(potassium titanyl phosphate) 레이저 치료기에서 냉각기의 수위 및 수온을 조절하는 센서로써 응용이 가능하다.

• 전자공학회논문지
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• 제51권10호
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• pp.210-212
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• 2014

전립선 질환은 대표적인 남성질환 중의 하나이다. 전립선 비대증은 전립선 질환 중의 하나로 배뇨 통증 등을 유발한다. 전립선 질환을 치료하기 위한 방법으로는 수술적, 약물적 방법 등이 있다. 그러나 이러한 방법은 역생성 자성 및 발기부전증 등의 후유증 및 합병증 발생한다. 이러한 문제를 해결하기 위해서 최근에는 온열 요법을 이용한 치료가 급증하고 있다. 본 연구에서는 이러한 온열 치료를 위해서 전립선의 해부학적 위치와 열선의 인체공학적으로 설계하여 시스템을 구성하였다. 본 연구에서 개발 된 시스템의 성능을 파악하기 위해서 돼지 조직을 이용한 열전달 성능을 검증하였다.

• Neonatal Medicine
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• 제15권2호
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• pp.172-175
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• 2008

혈관종은 신생아기에 가장 흔한 양성 종양으로 60% 이상이 두경부에 위치하며 예후가 좋으나 회음부나 항문 주위에 위치한 혈관종은 통증, 출혈, 반복적인 감염 및 궤양과 같은 합병증이 자주 동반된다. 특히 항문 주위에 위치한 혈관종에서 발생한 궤양은 스테로이드나 레이저등 다양한 치료에도 호전되지 않는 경우가 있다. 저자들은 일반적인 집중치료에 호전되지 않아 대장창냄술을 실시하였고 이후 궤양이 호전되는 과정을 경험하였기에 이를 보고하는 바이다.

• 대한두경부종양학회지
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• 제32권1호
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• pp.49-52
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• 2016

The lobular capillary hemangioma (LCH) was previously known to pyogenic granuloma and is benign vascular lesion which grows rapidly on skin and mucosa. It arises from whole body, but oral and nasal cavities are most predilection sites in the head and neck area. The laryngeal LCH looks like a granulomatous lesion of posterior glottis and its common etiology are tracheal intubation and laryngopharyngeal reflux disease etc. The LCH in larynx can cause blood tinged sputum and lump sense. The lesions refractory to medical therapy or causing dyspnea may require surgical excision. A 74-year-old man who presented gradually aggravated dyspnea, lump sensation and hoarseness of one month came to our hospital. The stroboscopic examination revealed large well-margined glottic mass. It was excised with $CO_2$ laser and finally diagnosed as LCH. We present a rare unique case of glottic LCH with a review of literatures.

• 대한후두음성언어의학회지
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• 제27권2호
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• pp.126-129
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• 2016

Rhabdomyosarcoma is an uncommon type of soft tissue malignant neoplasm characterized by undifferentiated mesodermal tissue. Sarcomas account for approximately 1% of all laryngeal neoplasm and rhabdomyosarcomas are the rarest sarcoma found in the larynx. When the sarcoma involves the larynx, radical surgery such as laryngectomy has been considered. With recent advances of combined therapy, however, it can be treated by conservative surgeries followed by postoperative radiotherapy and/or pulse chemotherapy. With reviews of literature, we report a 47-year-old patient complaining of husky voice and throat discomfort who was finally diagnosed as rhabdomyosarcoma of the vocal fold and successfully treated by laser cordectomy followed by adjuvant chemoradiotherapy.

• Journal of Oral Medicine and Pain
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• 제24권3호
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• pp.281-300
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• 1999

측두하악장애환자와 정상대조군의 두경부 근육에 있어서 실제 또는 위약 치료에 따른 저출력 레이저의 효과와 측두하악장애환자에서 저출력 레이저의 효과적인 치료기간을 평가하기 위하여, 측두하악장애를 가진 부산대학교 치과대학생 19명과 측두하악장애의 병력이나 증상이 없는 부산대학교 치과대학생 20명을 대상으로 치료전, 치료 2주 및 치료 4주에 구외촉진이 가능한 32 근골격 촉진점의 압력통각역치와 각각의 동통정도와 두개하악지수를 측정하여 다음과 갈은 결론을 얻었다, 1. 치료전 환자군의 압력통각역치는 정상대조군 압력통각역치보다 낮았다(p<0.05), 2. 환자군의 압력통각역치는 유의하게 증가하였으며 (p<0.05) 레이저 조사 환자군의 증가된 압력통각역치는 레이저 모의조사-환자군의 증가된 압력통각역치보다 더 크게 나타났다. 3. 대조군에서는 레이저 조사나 모의 조사에 의해 압력통각역치와 변화가 없었다. 4. 레이저 조사-환자군의 압력통각역치가 안면근육에서는 치료2주부터 유의하게 증가하였고 경부근육에서는 치료4주부터 유의하게 증가하였다(p<0.05) 5. 레이저 조사-환자군에서 동통의 정도와 두개하악지수는 유의하게 감소하였다(p<0.05).