• 제목/요약/키워드: Laser Scanning Microscope

검색결과 323건 처리시간 0.025초

4분할 photodiode를 이용한 scanning confocal microscope (Scanning confocal microscope using a quad-detector)

  • 유석진;김수철;이진서;권남익
    • 한국광학회지
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    • 제8권2호
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    • pp.165-168
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    • 1997
  • 780 nm의 반도체 레이저, compact disk의 광 pick-up용 actuator, 그리고 4분할 photodiode를 이용하여 scanning confocal microscope를 구성하여 시료면의 높이와 재질의 차이를 측정하였다. 4분할 photodiode에 검출되는 오차 신호를 이용하여 렌즈가 장착되어 있는 actuator를 움직이면서 시료면에 레이저 광속의 초점이 항상 위치하도록 하였으며, 이 때 actuator에 흐르고 있는 전류를 시료면의 높이로 나타내어 3차원 영상으로 표현하였다. 또한 재질의 차이는 4분할 photodiode에 검출되는 합산 신호를 이용하여 컬러 프린터에 나타나는 3차원 영상의 색을 다르게 나타내었다. 전체적인 크기도 30 mm * 20 mm * 20 mm 로서 작고 간단하며 scan 영역은 최대 1.6 mm * 1.6 mm이다. 반사광의 세기를 이용한 scanning confocal microscope의 영상과 4분할 photodiode에 검출되는 오차 신호를 적분하는 방식을 이용한 scanning confocal microscope의 영상을 구하여 그 차이를 비교하였다.

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Confocal Scanning Laser Microscope (CSLM)을 이용한 피부 기저층 멜라닌 밀도의 정량화 (Quantification of Melanin Density at Epidermal Basal Layer by Using Confocal Scanning Laser Microscope (CSLM))

  • 김동현;이승호;오명진;최고운;양우철;박장서
    • 대한화장품학회지
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    • 제40권3호
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    • pp.259-268
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    • 2014
  • 최근 여러 가지 비침습적인 방법으로 피부조직의 생검 또는 조직학적 처리과정 없이 피부의 실시간 이미지를 이용한 연구가 가능해졌다. Confocal scanning laser microscope (CSLM) 이미지는 830 nm의 근적외선 파장을 사용하는 비침습적 방법으로 피부의 내부 구조를 이미지화할 수 있으며, CSLM을 이용한 연구를 통해 비침습적인 피부 측정법으로 색소 침착 부위의 기저층과 진피 유두 사이에서 melanin cap, papillary ring과 같은 지표가 뚜렷하게 관찰된다고 보고되었다. 본 연구에서는 scanning probe image processor (SPIP)소프트웨어를 사용하여 CSLM 디지털 이미지 정량 분석을 처음 시도하였으며 본 연구결과는 정성적 피부조직 이미지 데이터를 정량화함으로써 향후 CSLM 활용범위를 높이게 될 것으로 기대하고 있다.

공초점 원리를 이용한 표면 현상 측정 (Surface measurement using Confocal principle)

  • 송대호;유원제;강영준;김경석
    • 한국정밀공학회:학술대회논문집
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    • 한국정밀공학회 2000년도 추계학술대회 논문집
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    • pp.51-54
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    • 2000
  • The traditional surface measuring method using confocal principle requires much time to measure an object surface since it is a scanning tool. In this paper, the upgraded confocal microscope is introduced. It is also a scanning tool but it requires 2D-scanning while the traditional one requires 3D-scanning. It means the time for measuring is considerably reduced. In addition, the measuring system is configured to increase the efficiency of beam. He-Ne laser whose frequency is 632.8nm is used for the laser source. An example of measuring result through the upgraded confocal microscope is showed.

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서브나노급 정밀도의 2 차원 원자현미경 개발 (Two Dimensional Atomic Force Microscope)

  • 이동연;권대갑
    • 대한기계학회:학술대회논문집
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    • 대한기계학회 2008년도 추계학술대회A
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    • pp.1778-1783
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    • 2008
  • A compact and two-dimensional atomic force microscope (AFM) using an orthogonal sample scanner, a calibrated homodyne laser interferometer and a commercial AFM head was developed for use in the nanometrology field. The x and y position of the sample with respect to the tip are acquired by using the laser interferometer in the open-loop state, when each z data point of the AFM head is taken. The sample scanner which has a motion amplifying mechanism was designed to move a sample up to $100{\times}100{\mu}m^2$ in orthogonal way, which means less crosstalk between axes. Moreover, the rotational errors between axes are measured to ensure the accuracy of the calibrated AFM within the full scanning range. The conventional homodyne laser interferometer was used to measure the x and y displacements of the sample and compensated via an X-ray interferometer to reduce the nonlinearity of the optical interferometer. The repeatability of the calibrated AFM was measured to sub-nm within a few hundred nm scanning range.

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우치치질에 레이저조사시 레진과의 결합강도에 관한 연구 (An Experimental Study on the Effect of Laser to Bond Strength between Composite Resin and Bovine Teeth)

  • 조주온;송광엽;박찬운
    • 대한치과보철학회지
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    • 제27권2호
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    • pp.155-172
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    • 1989
  • The purpose of this study is to measure the bonding strength at the enamel and dentin of extracted bovine incisors when they are irradiated by W Nd: YAG, EoQ Nd: YAG, and CW $CO_2$ laser, etched and then attached by composite resin. The laser-irradiated surface, the etched surface after the laser-irradiation and the interface of the treated surface and resin are observed by using scanning electron microscope. The NP Nd:YAG laser is used at the peam power of 207 KW. The EOQ Nd: YAG laser is used at the peak power of 1.15Mk. The CW $CO_2$ laser is used at the energy output of 5W and 10W in enamel; in dentin, at the energy output of 2W. The obtained results were as follows: 1. The shear bond strength of enamel decreased in the following order: EOQ Nd:YAG laser group, NP Nd:YAG laser group, CW $CO_2$ laser groups, unlased group. The unlased group showed a significant difference from EOQ Nd:YAG laser group (P<0.01), NP Nd:YAG laser group (P<0.05), but no significant difference from CW $CO_2$ laser group (P>0.05). 2. The shear bond strength of dentin decreased in the following order: CW $CO_2$ laser group, NP Nd:YAG laser group, unlased group, EOQ Nd:YAG laser group. The unlased group showed a significant difference from CW $CO_2$ laser group (P<0.01), but no significant difference from Nd:YAG laser groups (P>0.05). 3. The scanning electron microscope of enamel revealed irregular microcrack and pore at the surface in the NP Nd:YAG laser group and CW $CO_2$ laser group, but the crackless flat surface in the EOQ Nd:YAG laser group. 4. The scanning electron microscope of dentin revealed obstruction of in most of dentinal tubule and decrease of diameter. 5. The scanning electron microscope of the interface of resin and enamel revealed prominent penetration of resin tag in the EOQ Nd:YAG laser group.

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하이브리드형 AFM/SCM을 이용한 레이저 미세 가공 표면 측정 (Precision measurement of a laser micro-processing surface using a hybrid type of AFM/SCM)

  • 김종배;김경호;배한성;남기중;이대철;서운학
    • 한국레이저가공학회:학술대회논문집
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    • 한국레이저가공학회 2006년도 추계학술발표대회 논문집
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    • pp.123-127
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    • 2006
  • Hybrid type microscope with a Scanning Confocal Microscope (SCM) and a shear-force Atomic Force Microscope (AFM) is suggested and preliminarily studied. A image of $120{\times}120{\mu}m^2$ is obtained within 1 second by SCM because scan speed of a X-axis and Y-axis are 1kHz and 1Hz, respectively. Shear-force AFM is able to correctly measure the hight and width of sample with a resolution 8nm. However, the scan speed is slow and it is difficult to distinguish a surface composed of different kinds of materials. We have carried out the measurement of total image of a sample by SCM and an exact analysis of each image by shear-force AFM.

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펄스레이져 증착법을 이용한 $PbZrO_3/PbTiO_3$ 산화물 인공격자의 성장과 Scanning Force Microscope를 이용한 전기분극 특성 연구 (The Growth of $PbZrO_3/PbTiO_3$ Oxide Artificial Lattice Deposited by Pulsed Laser Deposition and Characterization of Ferroelectric Domain by Scanning Force Microscope)

  • 최택집;김진수;박배호;이재찬
    • 한국세라믹학회:학술대회논문집
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    • 한국세라믹학회 2004년도 춘계총회 및 연구발표회 초록집
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    • pp.37.1-37.1
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    • 2004
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공초점 레이저 주사 현미경을 이용한 단섬유 복합재료 사출 성형물 내의 섬유 배열 측정 및 수치모사 (Fiber Orientation in Injection-Molded Short Fiber Composites with a Confocal Laser Scanning Microscope and Numerical Simulation)

  • Lee, Kwang-Seok;Le, Seok-Won;Youn, Jae-Ryoun
    • 한국복합재료학회:학술대회논문집
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    • 한국복합재료학회 2001년도 춘계학술발표대회 논문집
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    • pp.201-204
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    • 2001
  • A Confocal Laser Scanning Microscope (CLSM) is applied to determine three-dimensional fiber orientation states in injection-molded short fiber composites. Since the CLSM optically sections the composites, more than two planes either on or below the surface of composites can be obtained. Therefore, three dimensional fiber orientation states are determined without destruction. To predict the orientation states, velocity and temperature fields are calculated by using a hybrid FEM/FDM method. The change of orientation state during packing stage is also considered by employing a compressible Hele-Shaw model. The predicted orientation states show good agreement with measured ones. However, some differences are found at the end of cavity. They may result from other effects, which are not considered in the numerical analysis.

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