• Journal of the Korean Geotechnical Society
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• v.21 no.9
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• pp.13-23
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• 2005

The prefabricated vertical drains (PVDs) are one of the most widely used techniques to accelerate the consolidation of soft clay deposits and dredged soil. Discharge capacity is one of the factors affecting the behavior of PVDs. In the field, a PVD is confined by clay or dredged soil, which is normally remolded during PVD installation. Under field conditions, soil particles may enter the PVD drainage channels, and the consolidation settlement of the improved subsoil may cause 131ding of the PVD. These factors will affect the discharge capacity of the PVDs. In this study an experimental study was carried out to estimate the discharge capacity of three different types of PVDs by utilizing the large-scale laboratory model testing and small-scale laboratory model testing equipments. The several factors such as confinement condition (confined by soft marine clay or dredged soil) and variations of the discharge capacity were studied with time under soil specimen confinement, The test results indicated that discharge capacity decreases with increasing load, time, and hydraulic gradient. With load application, the cross-sectional area of the drainage channel of PVD decreases because the filter of PVD is pressed into the core. The discharge capacity of the soft marine clay-confined PVDs is much lower than that of the dredged soil-confined PVDs.

• Bulletin of the Korean Chemical Society
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• v.28 no.6
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• pp.929-935
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• 2007

The objective of this study was to develop a rapid, simple, and qualitative acetylcholinesterase (AChE)- detection kit, based on a modification of the Ellman and ELISA methods, for the detection of organophosphorus (OP) and carbamate (CB) pesticide. The developed kits were used to screen a large number of agricultural samples (spiked and real) for OP and CB pesticide residues. AChE was extracted from the heads of honeybees (Apis mellifera L.) using Triton X-100, and was purified through 3 steps: diethylaminoethylcellulose chromatography (DEAE), affinity chromatography and membrane filtering, and Mono-Q column chromatography. Epoxy-activated Sepharose 6B affinity chromatography was used for large-scale purification. The presence of OP and CB pesticide residues in agricultural samples was assayed on the basis of AchE inhibition value. The presence (6 bands) or absence of some colored bands on the test line indicated a negative or positive result, respectively. The limits of detection for measured organophosphorus (OP) and carbamates (CB) pesticide residues in standard pesticide solutions and fortified samples were ranged from 0.50 to 2.50 ppm and 0.50 to 4.75 ppm, respectively.

• Proceedings of the Korean Geotechical Society Conference
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• 2000.11a
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• pp.41-48
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• 2000

Most land development projects and large scale civil works require a great amount of sand. And sand is also the most favorable material for soft ground improvement. The demand for sand is soaring rapidly due to increased number of projects regardless of its limited supply. Therefore, it is not difficult to predict that sand may become depleted and no longer be available as ground improvement material in the near future. Against this backdrop, developing an inexpensive sand-substitution material with an efficient accessibility will be necessary and urgently called for. So quick lime could be recommended as the substitutional material for sand. Quick lime is now preferred by forward developed nations. If Korea is able to take advantage of its abundant supply, economical efficiency could be achieved through massive production as well as being able to take advantage of utilization of natural resources. In this respect the purpose of this paper was to estimate improvement effect of soft ground though in-situ construction of quick lime pile. In-situ construction was peformed in road construction site of soft clay and in this study quick lime from Dan-yang that was estimated prominently in aspect of engineering characteristics was used. Quick lime piles were installed by 1.5m, 2.0m, 3.0m spacing to confirm improvement effect according to spacing and installed piles are 0.4m in diameter, up to 5m in length and the density of quick piles installed is 1.4 t/㎥. Vibrating wire pore water pressure cell was installed to confirm consolidation characteristics in surrounding of quick lime piles and both laboratory test and field test were carried out to confirm strength increase. In conclusion, soft ground improvement by quick lime piles was confirmed.

• Journal of Genetic Medicine
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• v.17 no.2
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• pp.68-72
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• 2020

Purpose: Trisomy 21, the cause of Down syndrome (DS) with various medical problems, is the most common aneuploidy during the fetal period. For diagnosis, a non-invasive screening test using maternal blood, which cannot be confirmed and invasive confirmation test with a risk of miscarriage, may be performed. The trophoblast retrieval and isolation of the cervix (TRIC) have been proposed by some researchers as an alternative to overcome the limitations of current tests. We experimented using TRIC to identify the possibility of trisomy 21 for the first time in Asia. Materials and Methods: Three cases of DS were analyzed confirmed by invasive tests (chorionic villus sampling, amniocentesis). All samples of trophoblasts immediately were immersed in phosphate-buffered saline and processed with formalin for fixation. The trophoblasts were isolated using an anti-human leukocyte antigen-G antibody coupled to magnetic nanoparticles. β-human chorionic gonadotropin (hCG)-expressing cells were considered as trophoblast cells, and the detection rate calculated. DS was confirmed by fluorescence in situ hybridization (FISH). Results: The mean trophoblast detection rate using β-hCG was 78.1%, and the detection rate using FISH was 22.2%. In all cases, the trisomy of chromosome 21 was identified. Conclusion: Trophoblast can be obtained from the five weeks of gestation and has a high detection rate, so it is noted that it can replace the current prenatal genetic test. To realize the clinical application as a prenatal genetic test, we will need additional efforts to identify trisomy 21 as well as other chromosomal abnormalities in future large-scale studies.

• Proceedings of the Korean Geotechical Society Conference
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• 2009.03a
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• pp.782-791
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• 2009

This study was carried out the laboratory tests and field plate load test in order to evaluate the reinforcement effect of geocell for road construction. The geocell-reinforced subgrade shows the increment of cohesion and friction angle with comprison of non-reinforced subgrade. In addition, the field plate load test was performed on the geocell-reinforced subgrade to estimate the bearing capacity of soil. The direct shear test was conducted with utilizing a large-scale shear box to evaluate the internal soil friction angle with geocell reinforcement. The number of cells in the geocell system is varied to investigate the effect of soil reinforcement. The theoretical bearing capacity of subgrade soil with and without geocell reinforcement was estimated by using the soil internal friction angle. The field plate load tests were also conducted to estimate the bearing capacity with geocell reinforcement. It is found out that the bearing capacity of geocell-reinforced subgrade gives 2 times higher value than that of unreinforced subgrade soil. In the future, the reinforcement effect of the geocell rigidity and load-balancing effect of the geocells should be evaluated.

• Proceedings of the Korean Geotechical Society Conference
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• 2008.10a
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• pp.117-125
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• 2008

The detection of thin-layered soil is important in soft soils to evaluate the soil behavior. The smaller diameter cone penetrometer have been commonly used to detect the layer with increasing sensitivity. The objective of this study is to detect the thin-layered soil using cone resistance and electrical resistance. The cone resistivity penetration test (CRPT) is developed to evaluate the cone tip resistance and electrical resistance at the tip. The CRPT is a micro-cone which has a $0.78cm^2$ in projected area. The application test is conducted in a laboratory large-scale consolidometer (calibration chamber). The kaolinite, sand and water are mixed to make the specimen at the liquid limit of 46% using a slurry mixer. It takes two months for the consolidation of the specimen. After consolidation, the CRPT test is carried out. Furthermore the standard CPT results are compared with the electrical resistance measured at the tip in the field. This study suggests that the CRPT may be a useful tool for detecting thin-layers in soft soils.

• Annals of Laboratory Medicine
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• v.38 no.6
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• pp.578-584
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• 2018

Background: Accurate, rapid, and cost-effective screening tests for hepatitis B virus (HBV) and hepatitis C virus (HCV) infection may be useful in laboratories that cannot afford automated chemiluminescent immunoassays (CLIAs). We evaluated the diagnostic performance of a novel rapid automated fluorescent lateral flow immunoassay (LFIA). Methods: A fluorescent LFIA using a small bench-top fluorescence reader, Automated Fluorescent Immunoassay System (AFIAS; Boditech Med Inc., Chuncheon, Korea), was developed for qualitative detection of hepatitis B surface antigen (HBsAg), antibody to HBsAg (anti-HBs), and antibody to HCV (anti-HCV) within 20 minutes. We compared the diagnostic performance of AFIAS with that of automated CLIAs-Elecsys (Roche Diagnostics GmbH, Penzberg, Germany) and ARCHITECT (Abbott Laboratories, Abbott Park, IL, USA)-using 20 seroconversion panels and 3,500 clinical serum samples. Results: Evaluation with the seroconversion panels demonstrated that AFIAS had adequate sensitivity for HBsAg and anti-HCV detection. From the clinical samples, AFIAS sensitivity and specificity were 99.8% and 99.3% for the HBsAg test, 100.0% and 100.0% for the anti-HBs test, and 98.8% and 99.1% for the anti-HCV test, respectively. Its agreement rates with the Elecsys HBsAg, anti-HBs, and anti-HCV detection assays were 99.4%, 100.0%, and 99.0%, respectively. AFIAS detected all samples with HBsAg genotypes A-F and H and anti-HCV genotypes 1, 1a, 1b, 2a, 2b, 4, and 6. Cross-reactivity with other infections was not observed. Conclusions: The AFIAS HBsAg, anti-HBs, and anti-HCV tests demonstrated diagnostic performance equivalent to current automated CLIAs. AFIAS could be used for a large-scale HBV or HCV screening in low-resource laboratories or low-to middle-income areas.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 2008.10a
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• pp.199-202
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• 2008

In this study, we investigated the thermal properties of $Zr_{66.4}Nb_{6.4}Cu_{10.5}Ni_{8.7}Al_{8.0}$ by using a differential scanning calorimeter (DSC), and then analyzed the composition of dendrite phase by using X-ray diffraction (XRD). A series of uniaxial compression tests has been performed under the strain rates between $10^{-5}/s$ and $10^{-2}/s$ at room temperature and near SLR. This BMGC has higher high temperature strength than other Zr-based monolithic BMGs because in-situ formed crystalline phases hinder a feasible viscous flow of amorphous matrix. Warm formability is also estimated by laboratory-scale extrusion test within supercooled liquid region. It was found that BMGC has poor formability compared with nother Zr-based bulk metallic glass composite presumably due to large volume fraction of 'brittle' crystalline phases distributed within amorphous matrix.

• Smart Structures and Systems
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• v.2 no.3
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• pp.275-293
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• 2006

For health monitoring purpose usually the structure is instrumented with a large scale and multichannel measurement system. In case of highway bridges, operating vehicle could be utilized to reduce the number of measuring devices. First this paper presents a static damage detection algorithm of using operating vehicle load. The technique has been validated by finite element simulation and simple laboratory test. Next the paper presents an approach of using this technique to field application. Here operating vehicle load data has been used by instrumenting the bridge at single location. This approach gives an upper hand to other sophisticated global damage detection methods since it has the potential of reducing the measuring points and devices. It also avoids the application of artificial loading and interruption of any traffic flow.

• Proceedings of the Korean Geotechical Society Conference
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• 2008.03a
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• pp.134-141
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• 2008

To evaluate the engineering properties of soil, the laboratory test always is carried out using samples obtained from the field. There are many studies to estimate the effect of sampling disturbance. The objective of this study appraises the disturbance using the shear wave velocity. The new shelby tube which three transducers are installed every 20cm interval is used. To laboratory tests, the large-scale consolidometer (calibration chamber) is used. During 1cm penetration, the shear wave velocity is measured by every transducer. The initial sampling disturbance is assessed through the velocity difference from bottom to right upside transducer. After finishing the sampling, the velocity is still measured every time to assess the soil disturbance in shelby tube itself. Through the measured velocity, the effect of disturbance is appraised. This study suggests that the sampling disturbance of shelby tube is effectively evaluated using shear wave velocity.