• 식물병연구
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• 제29권1호
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• pp.94-99
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• 2023

Blueberry red ringspot virus (BRRSV) and blueberry scorch virus (BlScV) are included in the quarantine virus list managed by the Korean Animal and Plant Quarantine Agency. A multiplex polymerase chain reaction (PCR) assay with an internal control was developed for the simultaneous detection of both viruses. The specific primers used here were designed based on the highly conserved regions of the genomic sequences of each virus, obtained from the National Center for Biotechnology Information nucleotide databases. The primers were designed to amplify a partial sequence within coat protein (CP) for detecting BRRSV and a partial sequence within the CP-16 kDa for detecting BlScV. 18S ribosomal RNA (rRNA) was used as internal control, and the primer set used in a previous study was modified in this study for detecting 18S rRNA. Each conventional PCR using the BRRSV, BlScV, and 18S rRNA primers exhibited a sensitivity of approximately 1 fg plasmid DNA. The multiplex PCR assay using the BRRSV, BlScV, and 18S rRNA primers was effective in simultaneously detecting the two viruses and 18S rRNA with a sensitivity of 1 fg plasmid DNA, similar to that of conventional PCR assays. The multiplex PCR assay developed in this study was performed using 14 blueberry cultivars grown in South Korea. BRRSV and BlScV were not detected, but 18S rRNA was all detected in all the plants tested. Therefore, our optimized multiplex PCR assay could simultaneously detect the two viruses and 18S rRNA in field samples collected from South Korea in a time-efficient manner. This approach could be valuable in crop protection and plant quarantine management.

• 한국작물학회지
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• 제68권4호
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• pp.422-430
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• 2023

우리나라 국민들에게 부족하기 쉬운 영양소인 칼슘함량이 풍부하고 항산화 활성이 높은 손가락조의 기능성 식품소재로서의 가능성을 확인하고자 손가락조 '핑거1호'를 이용하여 쿠키 가공을 하였을 때 품질 특성 및 칼슘 함량 등의 변화를 평가하여 신작물인 손가락조를 이용한 가공품 개발에 활용 여부를 판단하였다. 1) 밀가루 100%를 대조로하여 핑거1호 통곡 분말 10%, 30%, 50%, 100%를 첨가하여 쿠키를 제조하고 가공 특성과 이화학 특성 등, 품질과 항산화 활성 등을 조사하였다. 2) 핑거1호 분말 첨가량이 증가함에 따라 칼슘 함량은 10% 첨가시 1.8배에서 100%시 10.8배에 이르기까지 현저하게 증가하였다. 3) 반면, 나트륨의 경우 10% 첨가시 1.01배에서 100%시 1.38배로 증가량의 변화가 크지 않아 칼슘이 부족하고 나트륨은 과다섭취하는 우리나라 국민들의 식습관을 고려할 시 영양학적으로 바람직한 쿠키의 제조가 가능할 것으로 판단되었다. 4) 항산화활성도 칼슘 등 무기성분 함량증가와 마찬가지로 핑거1호 첨가량의 증가에 ABTS radical 소거활성 1.6~7.5배, DPPH radical 소거활성 2.8~18.7배 증가하였다. 5) 쿠키의 품질특성에서 핑거1호 분말첨가가 증가할수록 쿠키의 퍼짐성은 증가하고 경도가 감소되어 부드러운 조직감의 쿠키제조가 가능하였고, 색상도 10%의 첨가만으로도 뚜렷한 차이를 나타내었다.

• 한국농공학회논문집
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• 제49권2호
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• pp.87-97
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• 2007

The aim of this study was to compare crop contamination between two irrigation methods using microbial-contaminated water. The effect of relative humidity on microbial survival of the three indicator microorganisms was also investigated. Escherichia coli ATCC 25922, Clostridium perfringens ATCC 3624, and coliphage PRD1 were applied to irrigation water to grow cantaloupe, lettuce, and bell pepper. Half of the sixteen plots were subsurface drip irrigated (SDI) and the other half were furrow irrigated (FI). Two relative humidity levels were controlled at 15-65 % and 55-80 % for the dry and humid condition experiments, respectively. Samples of produce, surface soil, and subsurface soil at a depth of 10 cm were collected over a two-week period following the application of the study microorganisms. Overall, greater contamination of both produce and soil occurred in the FI plots. For the SDI plots, preferential water paths and resulting water appearance on the seed beds seemed to be responsible for produce contamination. Relative humidity levels did not appear to affect microbial survival in soil. PRD 1 showed lower inactivation rates than 5. coli in both dry and humid conditions. C. perfringens did not experience significant inactivation over the experimental period, suggesting this microorganism can be an effective indicator of fecal contamination.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.92-92
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• 2022

In preparation for the ever-changing climate and unification of North Korea and South Korea, it is necessary to increase the grain self-sufficiency rate by selecting crops with good utilization in high-altitude regions. The principle is to sow pastures at the end of August. However, sowing occurs in spring because the sowing period is missed when the weather is bad or when the workforce is insufficient. Sometimes when the grassland is completely devastated, it is frequently sowed in spring. In addition, North Korea consists of a high-altitude regions, and has been devastated in a general mountainous region. As a result, the landscape is not good and it is vulnerable to natural disasters such as landslides. Therefore, to prevent this, pasture must be sowed in the high-altitude regions. The goal of this study was to evaluate and compare yield and feed value of pasture species and varieties by spring sowing in high-latitude regions. The study was conducted in Pyeongchang, Gangwon-do, which is 700m height above sea level. The pasture species and varieties was sown on April 24, 2022. Each treatment was carried out by sowing 30 kg/ha, the experiment field size was 1 m²(1m×1m), and randomized block design with tri-repeat. The total of 14 varieties was used in the study, 6 varieties of Orchardgrass (OG), 6 varieties of Tall fescue (TF) and 2 varieties of Perennial ryegrass (PRG). The grassland composition fertilization using (N:P₂O₅:K₂O at 80:200:70 kg/ha) was conducted and management fertilizer was N:P₂O₅:K₂O at 210:150:180 kg/ha. The first harvest was June 26,2022 and the second harvest was on August 16, 2022. For statistical analysis of the data, an Analysis of Variance (ANOVA) was performed using the R3.6.3 software program, and all data was subjected to analysis using Duncan's multiple range test. Significance was set at the 5% level. The dry matter yield at the first harvest was the highest in PRG, and second harvest was the highest in TF (p < 0.05). Overall, PRG showed a trend of gradually decreasing growth, OG and TF showed a trend of gradually improving growth. This showed that PRG was considerably weaker to summer depression than other pasture species. Comparing the total dry matter yield, TF was the highest (4,565.45 kg/ha), but there was no significance difference with PRG (4,487.24 kg/ha) (p < 0.05). In addition, comparing the total TDN (total digestible nutrient) yield, TF was the highest (3147.33 kg/ha), second in PRG (2975.67 kg/ha) and third in OG (2052.33 kg/ha). Since this result is the data of the second harvests, if the result is derived by the end of next year, it will be provided as basic data for selection of pasture species and varieties suitable for spring seeding in high-altitude regions.

• 한국작물학회지
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• 제61권3호
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• pp.215-221
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• 2016

바이오에너지용 억새의 트랙터 부착 수확기 등 중장비 동원 늦은 수확에 따른 부작용을 구명하기 위해 전년도 생육 줄기의 늦은 수확 시기별 당년 생육특성과 마른줄기 수량을 구명한 결과는 다음과 같다. 1. 시험포장의 지하 10 cm 토양 경도는 맹아시, 맹아기, 맹아종 수확에서 각각 650, 793, 735 kPa로 일정한 경향이 없었으나 지하 20 cm에서는 각각 741, 915, 1,045 kPa로 늦게 수확할수록 단단해 졌다. 2. 이질 3배체에 비해 거대1호가 생육시기별 경수의 변이폭이 컸다. 거대 1호는 수확 시기별로 가장 늦은 맹아종에서 생육중기(5월 22일)에 169개/$m^2$로 가장 많았으나 생육 최성기(7월 22일)에 70개/$m^2$로 급격히 감소하였다. 3. 수확시기가 늦을수록 당년 생성된 지하경이 전년의 것에 비해 가늘어져 맹아종 수확에서 전년, 당년생성 지하경태가 거대 1호는 각각 9.2 mm, 6.7 mm 이질 3배체는 각각 8.5 mm, 8.0 mm였다. 4. 수확시기가 늦을수록 경장이 작아지고 아울러 줄기수량도 감소하였다. 거대1호와 이질3배체의 경장은 맹아시 수확에서 각각 308 cm, 374 cm였으나 맹아종 수확에서는 각각 268 cm, 341 cm로 작아졌다. 줄기 수량 또한 맹아시 수확에서는 각각 1,440 kg/10a, 1,777 kg/10a였으나 맹아종 수확에서는 각각 847 kg/10a, 963 kg/10a로 크게 감소하였다. 5. 출수율은 거대 1호의 경우 맹아시 수확에서 3.9%로 극히 낮았고, 맹아기 및 맹아종 수확에서는 거의 출수되지 않았다. 이질3배체는 맹아시 수확에서 49.6%였고, 맹아기, 맹아종에서도 각각 40.6%, 21.8% 수준을 유지하였다.

• 한국환경농학회지
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• 제26권1호
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• pp.25-35
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• 2007

인공습지나 수변녹지 및 완충 저류지와 같은 수질정화시설을 조성하는데 이용할 수 있는 수생식물을 선발하고자 낙동강 하류에 자생하는 수생식물과 외래 수생식물, 습생작물을 대상으로 부영양화 성분(T-N, T-P) 및 EC 관련 무기성분에 대한 정화능력을 검정하였다. 수생식물의 생육기간 동안 $225{\sim}444\;L\;m^{-2}$ 범위의 생활오폐수를 사용하였으며, 건물생산량이 많았던 벼와 부들, 줄, 비자루국화, 율무, 털물참새피 등이 350 L $m^{-2}$ 이상으로 가장 많은 생활하수를 소모하였다. 질소와 인의 체내흡수량과 실제 정화능력(정화효율 및 정화량) 및 수확 후 토양을 분석한 결과, 초장이 크면서 건물량도 많았던 벼, 비자루국화, 부들, 율무, 털물참새피, 줄 등이 부영양화성분에 대한 우수 정화식물로 선발되었다. 무기성분 중 K에 대한 정화력은 부영양화성분의 우수 정화식물로 선발된 수생식물과 더불어 물상추와 부레옥잠이 높은 결과를 보였다. EC와 관련이 높은 Ca와 Na, Cl에 대한 정화효과가 우수한 수생식물로는 물상추와 부레옥잠, 털물참새피가 선발되었으며, 특히 털물참새피는 $SO_4$에 대한 정화효과도 뛰어났다.

• 한국농공학회논문집
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• 제64권2호
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• pp.1-13
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• 2022

Agrivoltaic facility is the composite system that the solar panel is installed above the farmland, and it enables crop and electricity production simultaneously. Solar panels of the agrivoltaic facilities can block and reduce the amount of solar irradiance arriving at the farmland, but it can help the crop growth by preventing excessive solar irradiance. Therefore, to clarify how the agrivoltaic facilities affect the crop growth, precise solar irradiance distribution under the solar panel should be modeled. In this study, PAR (photosynthetically active radiation), radiation from 400 to 700 nm, which crops usually use to grow, was extracted from the total irradiance and its distribution model under various conditions was developed. Monthly irradiance distributions varied because the elevation of the sun was changed over time, which made the position changed that the local maximum and minimum irradiance appear. The higher panel height did not cause any significant difference in the amount of irradiance reaching below the solar panel, but its distribution became more uniform. Furthermore, the panel angles with the most irradiance arriving below the solar panel were different by month, but its difference was up to 2%p between the irradiance with 30° angle which is usually recommended in Korea. Finally, the interval between panels was adjusted; when the ratio of the length of the panel to the empty space was 1:2, the irradiance of 0.719 times was reached compared to when there was no panel, 0.579 times for 1:1 and 0.442 times for 2:1.

• 한국조경학회지
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• 제48권3호
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• pp.45-53
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• 2020

국제적으로 기후변화에 대한 대응 및 생물다양성 증진 방안이 대두되면서 국내 정부 및 공공기관에서는 수변구역 등 법정 보호지역 내 생태계를 보전하고 수질개선을 위해 사유지를 매수하여 보전·복원사업을 추진하고 있으나, 매수시스템 체계가 미흡하여 다양한 문제점을 초래하고 있다. 이에 본 연구에서는 동강유역 생태·경관보전지역을 중심으로 생태성 평가를 통한 효율적이고 체계적인 토지매수시스템을 개발하였다. 생태성 평가는 필지단위의 평가와 권역별 평가를 통합하여 생태성 평가모형을 개발하고, 생태기능 종합등급을 설정함과 동시에 현장검증을 통해 최종 등급화 하였다. 88필지의 매수토지에 대해 검증한 결과, 85.2%이상의 비교적 높은 일치성을 보였으며 불일치한 필지는 생태성 평가와 현장검증시 계절의 차이로 식생 고사, 경작물 수확 등 영향이 있었던 것으로 나타났다. 매수시스템은 멸종위기종과 핵심생태계에서의 거리를 기준으로 생태성 평가등급에 따라 1순위에서 9순위까지 순위를 선정하였으며, 동강유역 거운리, 문산리, 덕천리, 운치리, 귤암리, 가수리의 사유지를 대상으로 총 68필지에 대해 현장 검증 결과, 5순위까지 매수 우선순위가 체계적으로 도출되었다. 본 연구는 생태평가에 기반한 체계적인 토지매수시스템을 구축 및 제안함으로써 향후 보호지역 내 매수토지의 보전과 복원 및 생태적 관리에 일조할 것으로 기대된다.

• 한국약용작물학회:학술대회논문집
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• 한국약용작물학회 2011년도 춘계학술발표회
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• pp.122-123
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• 2011

• 식품과학과 산업
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• 제45권2호
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• pp.29-35
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• 2012

Japanese apricot (Prunus mume Sieb. et Zucc.) is the national symbol and was once the most important temperate fruit crop in Taiwan. Fruiting cultivars were originally introduced from southern China but commercial production was not significant until the 1970s. Currently 6,400 ha of Japanese apricot orchards distribute on shallow mountain hills in the central and the southern part of the island. Taiwanese commercial fruiting cultivars are plausibly chance seedlings or sports from the early introduction and are very low chilling required for budbreak. Ornamental cultivars have been mainly introduced from Japan but cultivations have been limited in high altitude area due to their high chilling requirement. In 2009, Taiwan Agricultural Research Institute's breeding program released the first low chill ornamental cultivar 'Tainung No.2' with a great ornamental potential in subtropical regions. Cultivation and production of Japanese apricot fruit in Taiwan continue to dwindle due to the declining Japanese market share. Ongoing industry transformation to increase domestic consumption and consumer's interest will sustain the future of Japanese apricot in Taiwan.