• 한국근적외분광분석학회:학술대회논문집
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• 한국근적외분광분석학회 2001년도 NIR-2001
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• pp.1629-1629
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• 2001

The knowledge of the nutrition in animal production is difficult to assess due of difficulty to determine the amount and quality of feeds intake, mainly if forages are the most important compound in the diet. It must be able to know responses to the metabolic process in lactating dairy cows earring out feeds evaluation trials. These metabolism studies with cows, requires measurements of: I) The amount of all feeds consumed. II) Excretion of faeces. III) Excretion of urine. Taking as a basis these trials, forage and total intake, dietary digestibility and balances of nitrogen and energy can be calculated. However, these feeds evaluation experiments with animals are very time consuming and expensives. The faeces excreted by animals containt undigested residues of the diet consumed. For this reason, their analysis can be an successful tool to determine the amount and quality of feed intake and other important biological parameters. The aim of this work was to know if faeces analysis by NIR could be used to determine with enough accuracy some attributes of different lactating dairy cows diets, using a global equation, developed on an heterogeneus population. For this purpose a total of 79 faecal samples from eight grass, three grass silages and two maize silages metabolic trials, on six cows each one, were used to constitute the initial population. The calibration equations were developed to predict forage and total intake, organic matter digestibility, digestibility coefficient of crude energy and digestibility energy. The combination of several trials with different diets and animal conditions gave promising results.

• Asian-Australasian Journal of Animal Sciences
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• 제27권7호
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• pp.960-964
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• 2014

An experiment was conducted to determine the effects of diets containing coarse-texture rice straw and small particle size alfalfa pellets as a part of total mixed ration (TMR) on milk productivity and chewing activity in lactating dairy cows. Sixteen multiparous Holstein dairy cows ($670{\pm}21kg$ body weight) in mid-lactation ($194.1{\pm}13.6$ days in milk) were randomly assigned to TMR containing 50% of timothy hay (TH) or TMR containing 20% of rice straw and 30% of alfalfa pellet mixture (RSAP). Geometric mean lengths of TH and RSAP were found to be 5.8 and 3.6, respectively. Dry matter intake, milk yield and milk composition were measured. Moreover, eating and ruminating times were recorded continuously using infrared digital camcorders. Milk yield and milk composition were not detected to have significant differences between TH and RSAP. Dry matter intake (DMI) did not significantly differ for cows fed with TH or RSAP. Although particle size of TH was larger than RSAP, eating, ruminating and total chewing time (min/d or min/kg of DMI) on TH and RSAP were similar. Taken together, our results suggest that using a proper amount of coarse-texture rice straw with high value nutritive alfalfa pellets may stimulate chewing activity in dairy cows without decreasing milk yield and composition even though the quantity of rice straw was 40% of TH.

• Parasites, Hosts and Diseases
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• 제62권1호
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• pp.64-74
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• 2024

This pilot study aimed to investigate the effects of regional and seasonal variations on the prevalence of Theileria orientalis and the hematological profile of non-grazed dairy cows in Korea. A total of 365 clinically healthy lactating Holstein Friesian cows from 26 dairy farms in 7 provinces that were categorized into northern, central, and southern regions were sampled during the warm period from July to August and the cold period from October to December. The detection of T. orientalis major piroplasm surface protein gene and the hematology non-grazed dairy cows were analyzed using peripheral blood samples. The T. orientalis prevalence was 20.0% (73/365). The prevalence in the southern region was 35.9%, which was significantly higher than that in the central (21.6%) and northern (12.9%) regions (P<0.05). The prevalence during warm period was higher (43.0%) than that during the cold season (13.5%). The infected cows showed significantly lower erythrocyte counts in the southern region (5.8±0.6 M/㎕) and during the warm period (5.8±0.7 M/㎕) compared with those in the central and northern regions and during the cold season, which affected the extended RBC parameters, including hematocrit and hemoglobin concentrations. Our findings revealed the prevalence of T. orientalis in Korea, highlighting its high occurrence during warm periods and in certain geographical regions. Climatic factors could contribute to the health and productivity of cattle, as evidenced by the prevalence of T. orientalis and its negative impact on animals.

• Asian-Australasian Journal of Animal Sciences
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• 제12권6호
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• pp.895-900
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• 1999

The aim of this study was to evaluate the effect of incorporating wet sorghum distiller's grains (WSDG) as part of their diet on the lactating performance of dairy cows. Twenty-seven Holstein milking cows were selected, all in the early lactating stage, with an average weight of 550 kg, and producing an average of 30 kg of milk daily. The cows were divided into three groups according to milk yield and lactation and were fed different total mixed rations. The diets were formulated according to NRC (1989) recommendations in three rations to (1) control diet, (2) 15% WSDG diet and (3) 30% WSDG diet. The three different diets were all formulated as iso-nitrogen and iso-energetic diets. After one week adaptation period, the experimental feeding was conducted for 8 weeks. Three ruminal cannulated cows were also examined in order to investigate ruminal fermentation of the three total mixed rations. The results showed that the milk yield, as corrected to the 4.0% fat standard, had no significant difference among the control, 15% WSDG and 30% WSDG treatment groups (p>0.05). The daily dry matter intake of the control group was higher than the other groups (p<0.05). with respect to milk composition, milk fat, milk protein and total solids, there was no significant difference among the treatment groups (p>0.05). The energy efficiency of the 30% WSDG group were significantly higher than the other treatment groups (p<0.05). Ruminal pH value showed no difference among the treatment groups (p<0.05). Ammonia-nitrogen concentration in the control group was higher than the other treatment groups (p<0.05). The concentration of total ruminal volatile fatty acid was similar in all three dietary groups.

• Journal of Animal Science and Technology
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• 제45권6호
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• pp.997-1006
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• 2003

본 연구는 우리나라에서 가축 장내발효에 의한 메탄배출 통계 기반을 구축하기 위해 수행되었다. 메탄 배출량을 추정하기 위해 가축은 소, 돼지, 가금, 면양, 산양으로 대분류하였고, 소는 한우와 젖소로 분류하여 각각 한우 송아지, 한우 비육우, 한우 번식우, 젖소 송아지, 젖소 비육우, 착유우로 세분류하였다. Tier 2 방법에 의한 메탄 배출계수는 각 세분류 집단의 가축의 생산성, 생체중, 도체중, 일당 증체량, 소화율을 근거로 하여 산출되었다. 한우의 배출계수는 39～49kg/head/year 정도로 IPCC에서 제시한 Tier 1의 47kg/head/year과 유사한 수준이었고, 젖소의 배출계수는 107 kg/head/year로 Tier 1 배출계수, 118kg/head/ year 보다 다소 낮게 나타났다. Tier 2 방법으로 추정한 가축 장내발효에 의한 메탄 배출량은 2001년, 총 126.8톤이었고 각 세분류별 메탄 배출량은 한우, 젖소, 돼지, 면양, 산양, 말에서 각각 61.70, 47.76, 13.08, 2.25, 0.17, 0.01 톤으로 나타났다. 본 연구에서 온실가스 배출추정 방법으로, Tier 1을 Tier 2로 대체하므로써 우리나라 가축 장내발효에 의한 메탄배출 통계의 정확성과 신뢰성이 개선된 것으로 사료된다.

• 한국유기농업학회지
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• 제21권4호
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• pp.711-723
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• 2013

지난 25년간 지구온난화로 인한 국내 기후변화 양상은 지역 간에 차이가 있어, 고온지역은 평균온도의 증가를 보이지는 않았으나, 최저기온의 지속적인 상승과 그에 따른 열대야 발생을 확인할 수 있었다. 또한 그로 인한 젖소의 고온스트레스 발생, 섭취량의 감소 및 생산성 저하가 예상된다. 저온지역의 경우에는 여름철 평균온도 및 최저온도의 상승으로 인해 연평균기온이 유의적으로 상승한 반면, 겨울철에는 오히려 최저기온의 지속적인 하강이 관찰됨에 따라 동물의 저온스트레스가 가중될 것으로 예상된다. 착유우가 저온스트레스 상태에 있을 때는 에너지요구량과 건물섭취량이 증가하여 생산 효율이 떨어지며, 사료효율의 감소로 경제성은 감소되고 우유 생산비는 증가한다. 특히, 극심한 저온스트레스 또는 사료, 음수 및 우사바닥의 결빙은 섭취량 감소를 야기하며 이에 따른 생산성의 저하는 더욱 가중될 것으로 예상된다. 결론적으로 지구온난화로 인한 기후변화에 따른 지역별 영향은 다르며, 온도 스트레스에 의한 낙농우의 생산성 저하를 최소화하고, 동물의 복지와 건강을 증진시키기 위해서는 지역별 기후변화 특성에 맞춘 사양기술의 개발이 필요하다.

• Asian-Australasian Journal of Animal Sciences
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• 제33권1호
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• pp.35-43
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• 2020

Objective: The aim of this research was to evaluate the effect of different concentrate levels in diets based on cactus Opuntia Stricta (Haw.) Haw cladodes on the performance of lactating Girolando cows. Methods: The experiment involved 10 Girolando multiparous dairy cows at 512.6 kg of body weight (BW) and producing 13.2 kg milk/d, allocated into two 5×5 Latin squares. The experimental treatments consisted of control diet composed by cactus Nopalea cochenillifera. Salm-Dyck. cladodes (Nopalea), forage sorghum silage and concentrate at 20% on dry matter (DM) basis, and four concentrate levels diets (20%, 24%, 28%, and 32%) plus cactus Opuntia stricta (Haw.) Haw. cladodes (Opuntia) and forage sorghum silage. Results: Regarding cows fed control diet, the nutrients intake were greater than for cows fed with cactus Opuntia and concentrate. Regarding concentrate levels, intakes of DM, organic matter (OM), crude protein (CP), non-fiber carbohydrates (NFC), and total digestible nutrients of cows increased linearly. Organic matter, CP, and NDF digestibilities were similar in between to control diet and cactus Opuntia-based diets. The digestibility of NFC increased linearly when the concentrate was inserted. The N balance was the same for control diet and cactus Opuntia-based diets, irrespective the concentrate levels. Conclusion: For cows producing 14 kg/d with 3.5% of fat, it is recommended 32% of concentrate to be included in cactus Opuntia-based diets, and the increase in concentrate level promotes a linear increase in milk yield.

• Asian-Australasian Journal of Animal Sciences
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• 제7권2호
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• pp.239-248
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• 1994

To study the relationship between certain hormones and metabolites and between hormones and milk yield during different stage of lactation, six lactating Karan Swiss cows and six Murrah buffaloes were maintained. Growth hormone, insulin, $T_3$, $T_4$, glucose, BHBA, NEFA and milk yield were studied. Highly negative relationship of growth hormone with insulin and triiodothyronine in cows and marginally negative in buffaloes suggest that insulin and triiodothyronine aid in the process of partitioning of nutrients towards milk production through reducing the demands of nutrients by peripheral tissue. The significant and negative correlation of growth hormone with dry matter intake in both the species suggest that the availability of nutrients from the digestive tract play a role in the regulation of growth hormone secretion. Positive relationship of growth hormone with non esterified fatty acids in both the species suggest that high growth hormone levels may result in fat mobilization and thereby increase the availability of energy precursors for milk synthesis. Insulin was negatively correlated with milk yield and lactose content and positively with milk fat and protein but the degree of relationship varied. In both the species the relationship between triiodothyronine and milk yield was negative and between thyroxine and milk yield was positive. However, it was significant only in cows and not in buffaloes. Thyroxine was positively correlated with beta-hydroxybutyrate and non-esterified fatty acids with milk yield in both the species.

• Asian-Australasian Journal of Animal Sciences
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• 제25권7호
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• pp.971-979
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• 2012

Four, lactating dairy crossbreds ($50%{\times}50%$ Holstein Friesian${\times}$Native Zebu cattle) were randomly assigned according to a $2{\times}2$ factorial arrangement (two protein levels and two levels of mangosteen peel pellets (Mago-pel)) in a $4{\times}4$ Latin square design to receive four dietary treatments. All cows received concentrate at a proportion of 1 kg concentrate per 2 kg of milk yield, and urea-treated 5% rice straw (UTRS) was given ad libitum. It was found that total dry matter intakes, nutrient digestibility, ruminal pH and $NH_3$-N concentrations were not affected (p>0.05) by treatments. Concentrations of ruminal pH and $NH_3$-N were not affected by dietary treatments although the concentration of BUN varied significantly (p<0.05) between protein levels (p<0.05). The populations of rumen bacteria and fungal zoospores did not differ among treatments (p>0.05); however, the population of protozoa was decreased (p<0.05) when cows received Mago-pel supplementation. The composition of the population of bacteria, identified by real-time PCR technique, including total bacteria, methanogens, Fibrobacter succinogenes and Ruminococcus albus was similar (p>0.05) among dietary treatments (p>0.05); however, copy numbers of Ruminococcus flavefaciens was increased when protein level increased (p<0.05). Microbial protein synthesis, in terms of both quantity and efficiency, was enriched by Mago-pel supplementation. Milk yield was greatest in cows fed UTRS based diets with concentrate containing protein at 16% CP with Mago-pel, but were lowest without Mago-pel (p<0.05). In addition, protein level and supplementation of Mago-pel did not affect (p>0.05) milk composition except solids-not-fat which was higher in cows fed the diet with 19% CP. Therefore, feeding a concentrate containing 16% CP together with 300 g/hd/d Mago-pel supplementation results in changes in rumen fermentation and microbial population and improvements in milk production in lactating dairy crossbreds fed on UTRS.

• 대한수의학회지
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• 제41권2호
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• pp.177-188
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• 2001

Mastitis is one of the most significant cause of economic loss to the dairy industry. Especially, Staphylococcus aureus is a major contagious mastitis-causing pathogen in dairy cattle. Because of its high transmission rate and resistance to antibiotic therapy, staphylococcal mastitis presents a constant threat to the dairy industry. Staphylococcal enterotoxin C(SEC) produced by S aureus has been known as one of superantigens which are able to stimulate a large proportion of T lymphocytes independently of their antigenic specificity. In this experiment, we have conducted preliminary studies with mice and lactating cows to evaluate the immunogenicity and safety of the experimental vaccine consists of SEC mutant antigen on controlling the bovine mastitis associated with S aureus infections. The average value of somatic cell counts in quarter milk, isolation rate of S aureus were consistently decreased in SEC-SER vaccinated groups, whereas antibody titers were highly increased in SEC-SER vaccinated groups. Peripheral blood were also collected from the lactating cows to determine the proportion of leukocyte subpopulation associated with humoral immunity(HI) and cell mediated immunity(CMI). Proportion of leukocyte subpopulation expressing $BoCD2^+$(total T lymphocyte), $BoCD4^+$(T helper cell), $BoCD8^+$(T cytotoxic/suppressor cell) and NonT/NonB lymphocyte which are involved in CMI in SEC-SER vaccinated groups were decreased for the initial stage after first vaccination and then increased from ten weeks after first vaccination maintaining elevated level till 14 weeks after vaccination. In contrast, proportion of monocyte, MHC class II and B lymphocyte which are associated with the production of primary immune response in SEC-SER vaccinated groups were increased for the initial period and then decreased from ten weeks after first vaccination. We present evidence that vaccination of SEC-SER mutant antigen in lactating cows induced a significant proliferation of bovine T lymphocytes. These results suggest that SEC-SER mutant antigen used in this experiment might be one of potential immunogen in developing innovative vaccine against bovine IMI associated with S aureus. Additional challenge trials should be carried out to evaluate substantial protection against S aureus under the commercial farm conditions.