• 제목/요약/키워드: Laboratory study

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ICP-MS와 AAS를 이용한 체내 혈청 Cu, Zn 분석에 관한 연구 (Analysis of Copper, Zinc in Serum Using ICP-MS & AAS)

  • 이예진;김동엽;이고은;조영숙
    • 대한임상검사과학회지
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    • 제41권1호
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    • pp.31-36
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    • 2009
  • The exposure of human beings to toxic trace metals (Cu, Zn) continues to be an important public health issue and concern. This study was conducted to assess the exposure to trace metals (Cu, Zn) in the general Korean population by inductively coupled plasma mass spectrometry (ICP-MS) and Atomic absorption spectrometry (AAS). Serum samples were obtained from 40 healthy volunteers. Specimens were collected in special container and we applied sample processing to minimize contamination. We used ICP-MS and AAS to analyze simultaneously the concentration of metals including copper, zinc. Distribution of trace metal levels in the general healthy population showed lower values. The results in this study can provide background data for clinical studies associated with trace metal exposure in the korean population.

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Detection of Waterborne Pathogens by PCR-reverse Blot Hybridization

  • Choi, Yeon-Im;Lee, Gyu-Sang;Bang, Hye-Eun;Kim, Jong-Bae;Lee, Hye-Young
    • 대한의생명과학회지
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    • 제16권1호
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    • pp.10-18
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    • 2010
  • The present study was set to develop comprehensive system for assessing the safety of drinking water using PCR-reverse blot hybridization assay (REBA). The REBA developed in this study can detect waterborne pathogens such as Shigella spp., Salmonella spp., Citrobacter spp., Enterobacter spp., Klebsiella spp., Yersinia spp., Mycobacterium spp., Listeria spp. at the genus level, and Escherichia coli, Citrobacter freundii, Klebsiella pneumoniae, Pseudomonas aeruginosa, Yersinia enterocolitica, Y. pseudotuberculosis, Mycobacterium avium complex, M. marinum, Enterococcus faecalis, and Staphylococcus aureus at the species level, and E. coli O157:H7 at the strain level.

수력 발전기 고정자 권선의 절연수명 평가기준 설정에 관한 연구 (A Study on the Evaluation Criteria for the Remaining Life of Hydro-Generator Stator Insulation)

  • 황돈하;김용주;김진봉;박명수;김희곤;이상중
    • 대한전기학회:학술대회논문집
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    • 대한전기학회 1996년도 하계학술대회 논문집 C
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    • pp.1769-1773
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    • 1996
  • The remaining life of generator stator winding has been the controversial issue amomg many experts in this area. The report from Japan claims that they can predict the remaining life of generator winding, while the North American has the negative opinion about it. This study aimed at verifying the validity of both Japanese criteria and North American argument on evaluation of generator winding insulation. Non destructive and destructive tests were performed on two hydro-generators. The test results showed that the trend analysis of stator winding insulation was the better option.

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제7차 교육과정을 고려한 중학교(中學校) 기술실(技術室)의 안전.시설.설비(安全.施設.設備) 배치(配置) 계획(計劃) (Facility Layout Planning for the Safety 7th Curriculum of Technology Laboratory in a Middle School)

  • 김종복;이상혁
    • 교육시설
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    • 제10권3호
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    • pp.15-26
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    • 2003
  • The purpose of this study is to describe the planning and applied safety facility layout for a technology laboratory in a middle school. A technological teaching facility has its essential purpose in meeting students' needs and achieving educational objectives efficiently and effectively. Practice-related appliances were selected through investigation of the current middle school laboratory, and analysis of the technology textbooks of the 7th curriculum. Laboratory layout was achieved with consideration of the teacher's and students need of safety and close proximity to laboratory facilities and equipment. Our recommendation takes the SLP survey results into consideration. The results of this study are as follows: The following floor plan is recommended. The facility layout will insure that students are able to work safely, in full view of academic instructors. Students will have easy access to all equipment due to the efficient floor plan and logical sequencing of equipment.

수술용 최대혈액신청량(MSBOS)의 설정 (Guidline for the Maximum Surgical Blood Order Schedule (MSBOS) for Surgical Operations)

  • 신경아;최종태;김현수
    • 대한임상검사과학회지
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    • 제36권2호
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    • pp.153-157
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    • 2004
  • The aim of our study is to determine maximum surgical blood order schedule (MSBOS) in each surgical operation through analyzing usage of blood products at Bundang Jesaeng General Hospital. We investigated the amount of transfused red cells for each operation and calculated crossmatching-to-transfusion ratio (C/T ratio) and MSBOS. This was accomplished by referring to the Laboratory Information System program during 1 year from January through December 2003. Coronary artery bypass surgery and decompressive craniectomy showed the highest MSBOS in our hospital. The average C/T ratio was 3.2 and excessive reservations for blood products have been made for many operations. From this study, guidelines for the optimal blood ordering for each surgery were suggested.

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무선 감시 시스템을 활용한 연구실 사고의 비상대응체계 개발에 대한 연구 (Study on Developing Emergency Response Procedure in Laboratory accident with Wireless Detecting System)

  • 이만수
    • 대한안전경영과학회지
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    • 제17권2호
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    • pp.139-145
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    • 2015
  • In these days, hazards in laboratories are consistently increasing due to high technological advance in modern science. Efforts to prevent accidents in laboratories became law named [ACT ON THE ESTABLISHMENT OF SAFE LABORATORY ENVIRONMENT]. The law made laboratories more safely but it's not enough to keep up laboratory safety in advanced countries. To improve laboratory safety, this study reformed laboratory emergency response flow chart based on each emergency scenarios and its evaluated hazards in laboratories applied wireless hazard detecting equipments.

Protective Effects of Acanthoic acid on Tertiary-Butyl Hydroperoxide or Carbon tetrachloride-Induced Liver Injury

  • Park, Eun-Jeon;Nan, Ji-Xing;Zhao, Yu-Zhe;Lee, Sung-Hee;Kim, Young-Ho;Nam, Jeong-Bum;Lee, Jung-Joon;Sohn, Dong-Hwan
    • 대한약학회:학술대회논문집
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    • 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.1
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    • pp.298.1-298.1
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    • 2003
  • The aim of this study was to investigate the protective effect of acanthoic acid on liver injury induced by either tertiary-butyl hydroperoxide (tBH) or carbon tetrachloride in vitro and in vivo. Acanthoic acid, (-)-pimara-9(11),15-diene-19-oic acid, is a diterpene isolated from the root bark of Acanthopanax koreanum. In in vitro study, the cellular leakage of lactate dehydrogenase (LDH) with 1.5 mM tBH for 1 j, were significantly inhibited by treatment with acanthoic acid(25 and 5mg/mL). (omitted)

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Protective Effect of Aqueous Extract from Erigeron annuus Against Cell Death Induced by Free Radicals

  • Myeongguk Jeong;Hyeokjin Kwon;Youngdon Ju;Go-Eun Choi;Kyung-Yae Hyun
    • 대한의생명과학회지
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    • 제28권4호
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    • pp.307-311
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    • 2022
  • The extract of EA lacks studies showing its efficacy other than that it contains caffeic acid, an active compound that has antioxidant and neuroprotective effects on nerve cells. Therefore, in this study, we attempted to determine the effectiveness of EA extraction. In this study, we performed a DPPH assay to determine the antioxidant potential of EA. And then, the cytotoxic concentration of EA in HaCaT keratinocytes was determined, and the antioxidant effect was determined by measuring the malondialdehyde (MDA). The results of DPPH, a chemical antioxidant assay, clearly demonstrated the antioxidant capacity of EA extracted with distilled water. In addition, cell-based assays provide useful information on the protective effect of EA on oxidative stress-induced apoptosis.

A Genetic Comparison of Brucella abortus Isolates from Animals and Humans by Using the MLVA Assay

  • Her, Moon;Kang, Sung-Il;Kim, Jong-Wan;Kim, Ji-Yeon;Hwang, In-Yeong;Jung, Suk-Chan;Park, Sang-Hee;Park, Mi-Yeoun;Yoo, Han-Sang
    • Journal of Microbiology and Biotechnology
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    • 제20권12호
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    • pp.1750-1755
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    • 2010
  • The MLVA assay is known to have a high ability to identify and discriminate Brucella species, so that it can be used as an epidemiological tool to discriminate Brucella isolates originating from restricted geographic sources. In this study, the genetic profiles of 38 B. abortus isolates from humans were analyzed and compared with genotypes from animal isolates in South Korea. As a result, it was found that they did not show high genetic diversity and were compacted. They were clustered together with animal isolates, showing a significant correlation to regional distributions. With its ability to prove a significant genetic correlation among B. abortus isolates from animals and humans in South Korea, the MLVA assay could be utilized as part of a program to control and eradicate brucellosis, one of the major zoonoses. This study represents the first data of genetic correlation of B. abortus isolates from humans and animals in South Korea.

Numerical simulation of natural convection around the dome in the passive containment air-cooling system

  • Chunhui Dong;Shikang Chen;Ronghua Chen;Wenxi Tian;Suizheng Qiu;G.H. Su
    • Nuclear Engineering and Technology
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    • 제55권8호
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    • pp.2997-3009
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    • 2023
  • The Passive containment Air-cooling System (PAS) can effectively remove the decay heat of the modular small nuclear reactor after an accident. The details of natural convection around the dome, which is a key part of PAS, were investigated numerically in the present study. The thermal dynamics around the dome were studied through the temperature, pressure and velocity contours and the streamlines. Additionally, the formation of the buoyant plume at the top of the dome was investigated. The results show that with the increase of Ra, the lift-off point moves toward the bottom of the dome, and the eddy under the buoyant plume grows larger gradually, which enhances the heat transfer. And the heat transfer along the dome surface with different truncation angles was investigated. As the angle increases, the heat transfer coefficient becomes stronger as well. Consequently, a newly developed heat transfer correlation considering the influence of truncation angle for the dome is proposed based on the simulated results. This study could provide a better understanding of natural convection around the dome of PAS and the proposed correlation could also offer more predictive value in the improvement of nuclear safety.