• Title/Summary/Keyword: Laboratory study

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An Analysis of Laboratory Instructions in Elementary School Science (초등학교 과학 실험 수업 분석)

  • Yang, Il-Ho;Jeong, Jin-Woo;Hur, Myung;Kim, Young-Shin;Kim, Jin-Soo;Cho, Hyun-Jun;Oh, Chang-Ho
    • Journal of Korean Elementary Science Education
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    • v.25 no.3
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    • pp.281-295
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    • 2006
  • The purpose of this study was to analyze laboratory instructions used in elementary school science lessons by utilizing an analysis instrument for science laboratory instruction(AISLI). This analysis instrument was comprised of 3 elements; the aim of the laboratory activity, the interaction generated, and the inquiry process. There were also a total of 20 sub-categories and its validity was identified at 0.89 by four science educators. For the purposes of this study, 90 laboratory instructions were video-recorded, then transcribed. Laboratory instructions were analyzed by three analyst teams, and the inter-rater reliability within teams was checked through Pearson correlation, with a score of 0.91. The results of this study were as follows: the two principle aims of laboratory activity, namely, to acquire declarative knowledge and to increase attitudes toward science, were observed to be in evidence in 98.9% and 92.2% in laboratory instructions, and the levels of categories of the interaction and inquiry processes were situated at level-1. The implications of these results are that laboratory instructions currently in use do not place sufficient demands upon students' scientific thinking and are not at all conducive to student-centered activities. Therefore in order to remedy this situation, the usefulness of current science laboratory instructions need to be re-evaluated.

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Xylazole inhibits NO-cGMP pathway in fetal rat nerve cells

  • Wang, Xinyu;Wu, Yue;Liu, Lin;Bai, Hui;Zhang, Zhiheng;Zhao, Mingchao;Ma, Tianwen;Song, Xiaopeng;Jia, Lina;Lv, Liangyu;Yu, Yue;Xu, Xinyu;Chen, Hong;Gao, Li
    • Journal of Veterinary Science
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    • v.23 no.1
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    • pp.16.1-16.13
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    • 2022
  • Background: Xylazole (Xyl) is a veterinary anesthetic that is structurally and functionally similar to xylazine. However, the effects of Xyl in vitro remain unknown. Objectives: This study aimed to investigate the anesthetic mechanism of Xyl using fetal rat nerve cells treated with Xyl. Methods: Fetal rat nerve cells cultured for seven days were treated with 10, 20, 30, and 40 ㎍/ mL Xyl for 0, 5, 10, 15, 20, 25, 30, 45, 60, 90, and 120 min. Variations of amino acid neurotransmitters (AANTs), Nitric oxide-Cyclic GMP (NO-cGMP) signaling pathway, and ATPase were evaluated. Results: Xyl decreased the levels of cGMP and NO in nerve cells. Furthermore, Xyl affected the AANT content and Na+-K+-ATPase and Ca2+-Mg2+-ATPase activity in nerve cells. These findings suggested that Xyl inhibited the NO-cGMP signaling pathway in nerve cells in vitro. Conclusions: This study provided new evidence that the anesthetic and analgesic effects of Xyl are related to the inhibition of the NO-cGMP signaling pathway.

Study on the Fourth Industrial Revolution and Clinical Laboratory Science Techniques (4차 산업혁명과 임상검사과학기술에 관한 연구)

  • Sung, Hyun Ho;Choi, Kwang-Mo;Jung, You Hyun;Cho, Eun Kyung
    • Korean Journal of Clinical Laboratory Science
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    • v.51 no.3
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    • pp.386-395
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    • 2019
  • The aim of this study was to introduce clinical laboratory science techniques with the core technology of the 4th Industrial Revolution. Among the core technologies of the 4th Industrial Revolution, AI, IOT, block-chain, robotics, and nanotechnology were analyzed and linked by themes. The scope of the job of clinical laboratory technologists (also known as medical laboratory technologists and medical technologists) is laboratory medicine testing, pathology testing, and clinical physiology testing. Through a number of previous papers, 73 linkages in the laboratory medicine area, 27 linkages in the pathology area, and 47 linkages in the clinical physiology area were examined. In the 4th industrial revolution and clinical laboratory science techniques, AI (4), IOT (3), block-chain (4), robotics (3) and nanotechnology (15) sectors were surveyed. The limitation of this study was the limitation in collecting and analyzing all the data and non-clinical areas were not analyzed. In addition, there was no validity test and no similar study. In conclusion, the core technologies of the 4th industrial revolution and clinical laboratory science techniques are closely related. Therefore, further research on the future and social benefits of clinical laboratory science techniques is needed.

A Study on the Space Area Guideline of Clinical Laboratory in Korea - Focused on Laboratory Case Studies (국내 종합병원 진단검사의학과의 면적산정 방법에 대한 연구 - 검사실 사례조사를 중심으로)

  • Kim, Youngaee;Song, Sanghoon
    • Journal of The Korea Institute of Healthcare Architecture
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    • v.26 no.2
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    • pp.39-47
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    • 2020
  • Purpose: Clinical laboratory of hospital has been demanded to extension or relocation regarding with the test number increase and analyzer development. Space area criteria per test numbers, lab functions, hospital bed and lab staffs are needed for draft space programing. So, the purpose of this study is to provide the space area guideline of clinical laboratory for space calculation in planning and design the spatial environment. Methods: Literature review has been used in checking the standards and guidelines. And questionnaire surveys to laboratory supervisors in hospitals have been conducted for the data collection. 60 answers have been analysed statistically by MS Excel program. Results: The result of this study can be summarized into three way calculations. The first one shows that the basic standard workspace and distance is applied in lab design. The second one shows that average space area criteria resulted from case studies is applied by 19㎡ per one staff, 0.9~1.0㎡ per one bed, and lastly linear length calculation of workbenches and analyzers on the bench top and floor mount, is multiplying it by the sum of the counter depth plus aisle width. Implications: In updating the space area calculation guidelines, it is necessary to cooperate with medical staffs and designers.

A study on the difference in safety awareness of research employees working for laboratory safety management of university institutes - University, Junior College, Polytechnic Colleges- (대학의 연구실 안전관리를 위한 연구활동 종사자의 안전의식 차이에 관한 연구 - 일반대학, 전문대학, 폴리텍대학 -)

  • Kwon, Yuna;Kwon, Young-Guk
    • Journal of the Korea Safety Management & Science
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    • v.17 no.3
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    • pp.89-96
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    • 2015
  • The study was conducted with statical analysis of data (828 data in 2010, 752 data in 2012, 648 data in 2014) in order to evaluate laboratory awareness difference of research employees working in different types of universities. Results of the study were as follows: First, university institutes in the order of polytechnic colleges, university, and junior college showed the highest laboratory safety awareness in 'awareness and education of laboratorial safety regulation' and 'awareness in laboratory risk factors'. Second, the difference in safety awareness of universities by year(years that conducted current status survey) was the highest in year 2014, then in 2010, and in 2008. Third, the difference of research employees working for laboratory safety management by year(years that conducted current status) showed that university had the highest laboratory safety awareness in year 2010, but it changed to polytechnic colleges in year 2012 and 2014. Through this study, we could recognize the difference in safety awareness of research employees working in university institutes.

Accident research II for the college students happened during Dental Laboratory Technology-major classes (치기공과 실습 중 안전사고 실태조사 II)

  • Park, Jong-Hee
    • Journal of Technologic Dentistry
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    • v.27 no.1
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    • pp.131-153
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    • 2005
  • The purpose of this study was to identify the accidents happened in the college dental laboratory technique classes and to realize the level of safety education for the dental laboratory technique major students. The research data were collected from a self-administered survey distributed to the conveniently selected college students whose majors were dental laboratory technology in Suwon, Daejeon, Daegu, Kimcheon, and Icsan. The study participants were 422 freshmen, sophomore and junior students in the selected cities. All collected survey responses were encoded and analyzed in SPSS 12.0. The findings were as follows. 1. The students who had any accidents in their laboratory classes were 36% and the other 64% answered 'no.' 2. Most injuries which the participants had were not significant. 3. The medical treatment periods of most injury cases were less than one week; however, approximately 20% of the self-reported injury cases reported that their treatment periods were longer than one week. 4. The body parts frequently injured from accidents were hands with 84.7% and arms, eyes, face, head, and legs in order. 5. Concerning the materials involved in the laboratory classes, most accidents happened when working with wax in 59.9%, with plaster in 52.3%, with wire in 39.8%, with alcohol in 34.6%, and casting iron in 22.2% in order. 6. The accident-prone laboratory methods were polishing with 53.5%, pickling or sanding with 17.4%, and casting or burning with 5.8% in order. 7. Most students wore their laboratory gowns well; however, few students wore mask before dust-involved jobs. Furthermore, only 57.1% students answered their laboratory fans worked during the polishing job. 8. Approximately 54% participant students answered that they took laboratory safety education when necessary only from the class instructors. 9. The 76.1% students responded that they needed laboratory safety education at least 1-2 times every semester. 10. The survey participants answered that the primary factors for injury prevention in their laboratory were taking safety education, wearing safety equipments, and providing safety-focused facilities. Concludingly, comprehensive evaluation and monitoring for potential risk factors from both the human side and the environment side should be continually provided to minimize the college laboratory accidents.

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Three Month Subacute Toxicity Study of Ginkgo Biloba Extract(EGb 761) in Rats

  • Lee, Yong-Soon;Nam, Jeong-Seok;Che, Jeong-Hwan;Lee, Suk-Man;Yang, Jae-Man;Kang, Byeong-Cheol;Lee, Hak-Mo;Park, Jae-Hak;Chai, Chan-Hee;Kang, Sung-An
    • Toxicological Research
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    • v.12 no.1
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    • pp.113-119
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    • 1996
  • Group of 40 male and 40 female Sprague-Dawley rats was given daily intravenous injections of different dosage of Ginkgo biloba extract(EGb 761), 7.5 mg/kg/day (low dosage group), 15 mg/kg/day (middle dosage group), or 30 mg/kg/day (high dosage group)for 3 month by tail vein according to Established Regulation of Korean National Institute of Safety Research (1994. 4. 14). Appearance, behavior, mortality, and food consumption of rats of treated groups were not affected during the experimental periods. No significant Ginkgo biloba extract(EGb 761)-related changes were found in urinalysis, hematology, serum chemistry, and organ weight. No histopathological lesions were seen in both control and treatment groups. Our results strongly suggest that no toxic changes were found in rat treated intravenously with Ginkgo biloba extract(EGb 761)for 3 month.

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