• Animal cells and systems
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• 제7권2호
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• pp.111-117
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• 2003

Ecological aspects of Cryptocercus kyebangensis life history were investigated via laboratory rearing and field observations. The number of antennal segments and head width were used to classify the first four instars. The results, which combine both the field collection and the laboratory rearing, indicate that eleven instars occur in C. kyebangensis. It supports the proposal on the number of instars of Park and Choe (2003c) based on field collections. A total of 388 nymps from 13 colonies were collected prior to winter to investigate overwintering stages. Of them,4％ (n = 17) were the second instars, 57％ (n = 220) were the third instars, and 39％ (n = 151) were the fourth instars, respectively. Thus, most of them overwinter in the third or fourth instars. The results indicate that young nymphs of C. kyebangensis have to reach at least 3rd or 4th instar to survive low temperature environment of winter. According to seasonal dynamics of populations, C. kyebangensis reaches adulthood in the summer of the fourth or fifth year (4-5 yr span) after their birth.

• 한국생명과학회:학술대회논문집
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• 한국생명과학회 2008년도 제49회 학술심포지움 및 국제학술대회
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• pp.27.1-27.1
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• 2008

• International Journal of Industrial Entomology and Biomaterials
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• 제47권1호
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• pp.1-11
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• 2023

The Indian golden muga silkworm, Antheraea assamensis Helfer is an economically important wild silkworm endemic to Northeastern part of India. In recent years, climate change has posed a threat to muga silk production due to the requirement that larvae be reared outdoors. Since the muga silkworm larvae are exposed to the vagaries of nature, the changing climate has increased the incidence of microbial diseases in the rearing fields. Accurate diagnosis of the disease causing pathogens and its associated epidemiology are prerequisites to manage the diseases in the rearing field. Although conventional microbial culturing methods are widely used to identify pathogenic bacteria, they would not provide meaningful information on a wide variety of silkworm pathogens. The information on use of molecular diagnostic tools in detection of microbial pathogens of wild silk moths is very limited. A wide range of molecular and immunodiagnostic techniques including denaturing gradient gel electrophoresis (DGGE), random amplified polymorphism (RAPD), 16S rRNA/ITSA gene sequencing, multiplex polymerase chain reaction (M-PCR), fluorescence in situ hybridization (FISH), immunofluorescence, and repetitive-element PCR (Rep-PCR), have been used for detecting and characterizing the pathogens of insects with economic significance. Nevertheless, the application of these molecular tools for detecting and typing entomopathogens in surveillance studies of muga silkworm rearing is very limited. Here, we discuss the possible application of these molecular techniques, their advantages and major limitations. These methods show promise in better management of diseases in muga ecosystem.

• 한국발생생물학회지:발생과생식
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• 제1권2호
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• pp.173-180
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• 1997

This study has been conducted to investigate the molting behavior and the effects of rearing temperature and day length on molting in the behavior and the effects of rearing temperature and day length on molting in the giant freshwater prawn, Macrobrachium rosenbergii reared in the laboratory. The results obtained were summarized as follow: 1. After pre-spawning molting, the protopodites of 1st, 2nd, 3rd, and 4th except 5th pleopod bore new breeding setae which conserve eggs in the brooding chamber and the basis of 3rd, 4th, and 5th pereiopods bore new breeding dresses which transport the ovulated eggs into the brooding chamber. 2. Adult females reared in 27.5-$29.4^{\circ}C$ molted 10-12 times per year at interval of 27-35 days, of which four or six moltings were common molting for growth and another four or five moltings were pre-spawning molting for spwaning and brooding. In winter season, pre-spawning molting did not happen to most of adult females in spite of the same temperature. 3. Duration of intermolt cycle was 31-38 days and 26-30 days at 25.3- $26.5^{\circ}C$ and 28.7- $30.4^{\circ}C$ of rearing temperature, respectively.

• 한국양식학회지
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• 제12권4호
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• pp.267-274
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• 1999

A rotifer starin has been isolated from tide pools located near Sogwipo, Cheju Island and the effects of environmental factors (water temperature and salinity) and clonal differences have been examined with regard to size variation and cyclomorphosis. Adult female rotifers varied from 141.61 to $173.97 \mutextrm{m}$ in their mean lorica length under different rearing conditions in the laboratory and also had pointed anterior spines . Thus, this strain was classified as Brachionus rotundiformes. Mean lorica length tended to be smaller as rearing temperature increased although there were some exceptions. Statistical analysis indicated that lorica length was largely influenced by both clonal differences and rearing temperatures although the former as a genetic factor affected lorica length a little more than the latter did ; overall effect of salinity on lorica length was not statistically signicicant; furthermore, cyclomorphosis was also largely influenced by both clonal differences and rearing temperatures, but clonal differences as a genetic factor affected rotifer cyclomorphosis much more than temperature did.

• International Journal of Industrial Entomology and Biomaterials
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• 제29권2호
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• pp.198-206
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• 2014

The olive fly Bactrocera oleae (Rossi) is the key pest for olive cultivation worldwide. Substantial effort has been invested in the development of the sterile insect technique (SIT) to control this pest. One of the limitations to develop SIT technology for olive fruit fly is the low ability of wild females to lay eggs in other medium than olive fruits, and their slow adaptation to oviposition in artificial substrates. In the present study, fruit grapes were used as an alternative egg collection medium to harvest eggs and young larvae from freshly colonized wild strains originating from France, Italy, Spain and Croatia. The larvae were allowed to develop into the fruits until the second instar, before they were extracted out and further reared on a standard artificial diet. Furthermore, F1 to F4 female flies were alternatively offered wax bottles to oviposit. Finally, the performance of hybrid strains created from crosses between wild and long colonised flies was assessed. The results showed that females of all 4 wild strains readily oviposited eggs in grapes and from the F2 generation onward, females from all strains were adapted to laying eggs in wax bottles. No difference was observed in eggs and pupae production among all strains tested. The findings are discussed for their implications on SIT application against olive fruit fly.

• 한국응용곤충학회지
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• 제44권4호
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• pp.293-298
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• 2005

규격이 다른 5가지 사육용기, A용기 (원통형, 직경 10 cm, 높이 4 cm), B용기 (원통형, 지름 14.5 cm, 높이 2.8 cm), C용기 (사각통, 가로${\times}$세로 : $6.5{\times}6.5cm$, 높이 10 cm), D용기 (원통형, 밑지름 9 cm, 밑지름 11.5 cm,높이 10.8 cm), E용기 (원통형, 직경 15 cm, 높이 7.5 cm)에 두류종자 (대두+땅 콩종자) 및 스폰지급수물을 제공하여 $24^{\circ}C$, 16L:8D 실내조건에서 가로줄노린재의 부화율, 우화율 및 산란수를 조사하여 증식율을 구하였다. 난기간은 사육용기에 관계없이 6일 이었다. 부화율은 $63{\sim}80%$로 B용기에서 가장 높았다. 영기별 발육기간은 1령에서 5일, 5령에서 11일로 영기가 증가할수록 현저히 증가하였다. 약충기간은 $35{\sim}36$일로 사육용기에 따른 현저한 차이는 없었다. 우화을은 $65{\sim}82%$로 B용기에서 가장 높았으며, 다음은 A용기 였다. 성충수명은 수컷에서 $35{\sim}83$일, 암컷에서 $32{\sim}79$일 이었고, 성별에 관계없이 B용기에서 가장 길었다. 암컷의 산란수는 $38{\sim}86$개로 사육용기에 따라 현저한 차이가 있었으며, B용기에서 가장 많았고, E용기에서 가장 적었다. 따라서 가로줄노린재의 1세대 증식율은 약 $17{\sim}56$배로 사육용기에 따른 현저한 차이와 함께 B용기에서 가장 높았다. 그러므로 실내에서 가로줄노린재를 가장 안정적으로 사육하기 위해선 B용기를 이용하는 것이 가장 효과적인 것으로 나타났다.

• Animal cells and systems
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• 제12권3호
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• pp.171-180
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• 2008

The complete larval development of Pagurus japonicus(Stimpson) is described, based on laboratory rearing. The species has four zoeal stages and a megalopa. The larvae are described and illustrated, and detailed comparisons with other pagurid larvae in the Korean coast are made.

• Animal cells and systems
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• 제14권2호
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• pp.115-123
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• 2010

The complete larval development of Rhinolithodes wosnessenskii Brandt, 1848 is described, based on laboratory rearing. The species has four zoeal stages and a megalopa. The zoeas of R. wosnessenskii are readily distinguished from all other described zoeas of the subfamily Lithodinae by having a middorsal spine of the posterior margin of the carapace.

• 생태와환경
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• 제39권3호통권117호
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• pp.413-417
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• 2006

한국의 산간계류에 흔히 서식하는 단각류인 보통옆새우의 실내 사육에 필수적 환경요인인 배양액과 온도조건에 대한 실험실내 실험을 실시하였다. 실험에 이용한 보통옆새우는 2003년 12월 경기도 가평천의 옆에 위치한 한 작은 샘의 유출부로부터 채집하여 사용하였다. 보통옆새우의 생존율은 사육 배양액 (증류수, 수돗물, 깔따구 배양액 및 하천수)과 사육 온도(10, 15, 20, $25^{\circ}C$)에 대한 영향으로 평가하였다. 그 결과로서, 보통옆새우는 수돗물, 하천수, 깔따구 배양액, 증류수의 순으로 그리고 10, 15, 20, $25^{\circ}C$의 순으로 높은 생존율을 나타냈다.