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Microorganism penetration in dentinal tubules of instrumented and retreated root canal walls. In vitro SEM study

  • Al-Nazhan, Saad;Al-Sulaiman, Alaa;Al-Rasheed, Fellwa;Alnajjar, Fatimah;Al-Abdulwahab, Bander;Al-Badah, Abdulhakeem
    • Restorative Dentistry and Endodontics
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    • v.39 no.4
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    • pp.258-264
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    • 2014
  • Objectives: This in vitro study aimed to investigate the ability of Candida albicans (C. albicans) and Enterococcus faecalis (E. faecalis) to penetrate dentinal tubules of instrumented and retreated root canal surface of split human teeth. Materials and Methods: Sixty intact extracted human single-rooted teeth were divided into 4 groups, negative control, positive control without canal instrumentation, instrumented, and retreated. Root canals in the instrumented group were enlarged with endodontic instruments, while root canals in the retreated group were enlarged, filled, and then removed the canal filling materials. The teeth were split longitudinally after canal preparation in 3 groups except the negative control group. The teeth were inoculated with both microorganisms separately and in combination. Teeth specimens were examined by scanning electron microscopy (SEM), and the depth of penetration into the dentinal tubules was assessed using the SMILE view software (JEOL Ltd). Results: Penetration of C. albicans and E. faecalis into the dentinal tubules was observed in all 3 groups, although penetration was partially restricted by dentin debris of tubules in the instrumented group and remnants of canal filling materials in the retreated group. In all 3 groups, E. faecalis penetrated deeper into the dentinal tubules by way of cell division than C. albicans which built colonies and penetrated by means of hyphae. Conclusions: Microorganisms can easily penetrate dentinal tubules of root canals with different appearance based on the microorganism size and status of dentinal tubules.

Development of an Engine Simulator for Optimal Control System Implementation of a Gas Turbine Engine (가스터빈엔진 최적 제어시스템 구현을 위한 엔진 시뮬레이터 개발)

  • Cha, Young-Bum;Koo, Bon-Min;Song, Do-Ho;Choi, Jung-Keyng
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.11 no.1
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    • pp.75-82
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    • 2007
  • In advanced countries, a gas turbine engine is developed to use in aircraft, vessels, and target weapons. Our nation also passed the level of producing engine components and now, we are developing small-sized gas turbine engine. The most important point of the gas turbine engine, the engine control technique, is evaded by the advanced nations. This document contains the research about the development of the gas turbine engine simulator. The simulator presented in this document has a mathematical engine model based on a capacity data of the gas turbine engine to advance the engine simulator. Through this process, it eases the development of the gas turbine engine control algorithm and helps to check the engine controller function. In this simulator, the engine sensor signal conversion board is designed, so the engine model shows like a real sensor signal during the simulation. Also, this paper contrasts the actual engine test with the simulation results to verify the performance.

Development of Strain-gauge-type Rotational Tool Dynamometer and Verification of 3-axis Static Load (스트레인게이지 타입 회전형 공구동력계 개발과 3축 정적 하중 검증)

  • Lee, Dong-Seop;Kim, In-Su;Lee, Se-Han;Wang, Duck-Hyun
    • Journal of the Korean Society of Manufacturing Process Engineers
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    • v.18 no.9
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    • pp.72-80
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    • 2019
  • In this task, the tool dynamometer design and manufacture, and the Ansys S/W structural analysis program for tool attachment that satisfies the cutting force measurement requirements of the tool dynamometer system are used to determine the cutting force generated by metal cutting using 3-axis static structural analysis and the LabVIEW system. The cutting power in a cutting process using a milling tool for processing metals provides useful information for understanding the processing, optimization, tool status monitoring, and tool design. Thus, various methods of measuring cutting power have been proposed. The device consists of a strain-gauge-based sensor fitted to a new design force sensing element, which is then placed in a force reduction. The force-sensing element is designed as a symmetrical cross beam with four arms of a rectangular parallel line. Furthermore, data duplication is eliminated by the appropriate setting the strain gauge attachment position and the construction of a suitable Wheatstone full-bridge circuit. This device is intended for use with rotating spindles such as milling tools. Verification and machining tests were performed to determine the static and dynamic characteristics of the tool dynamometer. The verification tests were performed by analyzing the difference between strain data measured by weight and that derived by theoretical calculations. Processing test was performed by attaching a tool dynamometer to the MCT to analyze data generated by the measuring equipment during machining. To maintain high productivity and precision, the system monitors and suppresses process disturbances such as chatter vibration, imbalances, overload, collision, forced vibration due to tool failure, and excessive tool wear; additionally, a tool dynamometer with a high signal-to-noise ratio is provided.

Reliable Measurement and Analysis System for Ubiquitous Healthcare (고신뢰 유비쿼터스 헬스케어 데이터 측정 및 분석 시스템)

  • Jung, Sang-Joong;Seo, Yong-Su;Kim, Jong-Jin;Chung, Wan-Young
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2009.05a
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    • pp.293-297
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    • 2009
  • This paper describes a real-time reliable measurement and analysis system for ubiquitous healthcare based on IEEE802.15.4 standard. In order to obtain and monitor physiological body signals continuously, wearable pulse oximeter is designed in wrist that could used to measure oxygen saturation of a patient unobtrusively. The measured data was transferred to a central PC or server by using wireless sensor nodes via a wireless sensor network for storage and analysis purposes. LabVIEW server program was designed to monitor and process the measured photoplethysmogram(PPG) to accelerated plethysmogram(APG) by appling second order derivatives in server PC. These experimental results demonstrate that APG can precisely describe the features of an individual's PPG and be used as estimation of vascular elasticity for blood circulation.

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The Development of Structural Test Facility for the Strength Assessment of CFRP Marine Leisure Boat (탄소섬유강화플라스틱 재료 레저선박의 구조강도 평가를 위한 시험설비 구축과 운용에 관한 연구)

  • Jeong, Han Koo;Zhang, Yang;Yum, Deuk Joon
    • Journal of the Society of Naval Architects of Korea
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    • v.54 no.4
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    • pp.312-320
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    • 2017
  • This paper deals with the development of structural test facility for the strength assessment of marine leisure boat built from carbon fiber reinforced plastics (CFRP) materials. The structural test facility consists of test jig, load application and control system, and data acquisition system. Test jig, and load application and control system are designed to accommodate various size and short span to depth ratios of single skin, top-hat stiffened and sandwich constructions in plated structural format such as square and rectangular shapes. A lateral pressure load, typical and important applied load condition to the plates of the hull structure for marine leisure boat, is simulated by employing a number of hydraulic cylinders operated automatically and manually. To examine and operate the structural test facility, five carbon/epoxy based FRP square plates having the test section area of $1m^2$, which are part of CFRP marine leisure boat hull, are prepared and they are subjected to monotonically increasing lateral pressure loads. In the test preparation, considering the symmetry of the plates geometry, various strain gauges and linear variable displacement transformer are used in conjunction with data acquisition system utilizing LabVIEW. From the test observation, the responses of the CFRP hull structure of marine leisure boat are understood by obtaining load to deflection and strain to load curves.

Mechanical and thermodynamic stability, structural, electronics and magnetic properties of new ternary thorium-phosphide silicides ThSixP1-x: First-principles investigation and prospects for clean nuclear energy applications

  • Siddique, Muhammad;Iqbal, Azmat;Rahman, Amin Ur;Azam, Sikander;Zada, Zeshan;Talat, Nazia
    • Nuclear Engineering and Technology
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    • v.53 no.2
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    • pp.592-602
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    • 2021
  • Thorium compounds have attracted immense scientific and technological attention with regard to both fundamental and practical implications, owing to unique chemical and physical properties like high melting point, high density and thermal conductivity. Hereby, we investigate the mechanical and thermodynamic stability and report on the structural, electronic and magnetic properties of new silicon-doped cubic ternary thorium phosphides ThSixP1-x (x = 0, 0.25, 0.5, 0.75 and 1). The first-principles density functional theory procedure was adopted within full-potential linearized augmented plane wave (FP-LAPW) method. The exchange and correlation potential terms were treated within Generalized-Gradient-Approximation functional modified by Perdew-Burke-Ernzerrhof parameterizations. The proposed compounds showed mechanical and thermodynamic stable structure and hence can be synthesized experimentally. The calculated lattice parameters, bulk modulus, total energy, density of states, electronic band structure and spin magnetic moments of the compounds revealed considerable correlation to the Si substitution for P and the relative Si/P doping concentration. The electronic and magnetic properties of the doped compounds rendered them non-magnetic but metallic in nature. The main orbital contribution to the Fermi level arises from the hybridization of Th(6d+5f) and (Si+P)3p states. Reported results may have potential implications with regard to both fundamental point of view and technological prospects such as fuel materials for clean nuclear energy.

Study on IoT-based Map Inside the Building and Fire Perception System (IoT 기반 건물 내부 지도 및 화재 안내 시스템에 관한 연구)

  • Moon, Sung-Ryong;Cho, Joon-Ho
    • Journal of Digital Convergence
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    • v.17 no.1
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    • pp.85-90
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    • 2019
  • This paper is a study on IoT based map inside the building and fire perception system using microprocessor and LABVIEW program. The smart control system implemented in this paper is designed to identify the location of fire by using microprocessor, flame detection sensor, carbon monoxide sensor and temperature sensor, and to guide the optimal travel route through Zigbee communication. And the proposed system uses QR code to interoperate with smartphone. The coordinator control verified that the sensor value of the smart control system installed through the LABVIEW software was confirmed. The IoT based control system studied in this paper was implemented with Arduino mega board and LABVIEW software, and the operation status was confirmed by display device and coordination.

Reliability verification of cutting force experiment by the 3D-FEM analysis from reverse engineering design of milling tool (밀링 공구의 역 공학 설계에서 3D 유한요소 해석을 통한 절삭력 실험의 신뢰성 검증)

  • Jung, Sung-Taek;Wi, Eun-Chan;Kim, Hyun-Jeong;Song, Ki-Hyeok;Baek, Seung-Yub
    • Design & Manufacturing
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    • v.13 no.2
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    • pp.54-59
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    • 2019
  • CNC(Computer Numerical Control) machine tools are being used in various industrial fields such as aircraft and automobiles. The machining conditions used in the mold industry are used, and the simulation and the experiment are compared. The tool used in the experiment was carried out to increase the reliability of the simulation of the cutting machining. The program used in the 3D-FEM (finite element method) was the AdvantEdge and predicted by down-milling. The tool model is used 3D-FEM simulation by using the cutting force, temperature prediction. In this study, we carried out the verification of cutting force by using a 3-axis tool dynamometer (Kistler 9257B) system when machining the plastic mold Steel machining of NAK-80. The cutting force experiment data using on the charge amplifier (5070A) is amplified, and the 3-axis cutting force data are saved as a TDMS file using the Lab-View based program using on NI-PXIe-1062Q. The machining condition 7 was the most similar to the simulation and the experimental results. The material properties of the NAK-80 material and the simulation trends reflected in the reverse design of the tool were derived similarly to the experimental results.

A Portable Impedance Spectroscopy Instrument for the Measurement of the Impedance Spectrum of High Voltage Battery Pack (고압 배터리 팩의 임피던스 스펙트럼 측정용 휴대용 임피던스 분광기)

  • Rahim, Gul;Choi, Woo-Jin
    • The Transactions of the Korean Institute of Power Electronics
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    • v.26 no.3
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    • pp.192-198
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    • 2021
  • The battery's State of Health (SOH) is a critical parameter in the process of battery use, as it represents the Remaining Useful Life (RUL) of the battery. Electrochemical Impedance Spectroscopy (EIS) is a widely used technique in observing the state of the battery. The measured impedance at certain frequencies can be used to evaluate the state of the battery, as it is intimately tied to the underlying chemical reactions. In this work, a low-cost portable EIS instrument is developed on the basis of the ARM Cortex-M4 Microcontroller Unit (MCU) for measuring the impedance spectrum of Li-ion battery packs. The MCU uses a built-in DAC module to generate the sinusoidal sweep perturbation signal. Moreover, it performs the dual-channel acquisition of voltage and current signals, calculates impedance using a Digital Lock-in Amplifier (DLA), and transmits the result to a PC. By using LabVIEW, an interface was developed with the real-time display of the EIS information. The developed instrument was suitable for measuring the impedance spectrum of the battery pack up to 1000 V. The measurement frequency range of the instrument was from 1 hz to 1 Khz. Then, to prove the performance of the developed system, the impedance of a Samsung SM3 battery pack and a Bexel pouch module were measured and compared with those obtained by the commercial instrument.

Neuroprotective Effect of Epalrestat on Hydrogen Peroxide-Induced Neurodegeneration in SH-SY5Y Cellular Model

  • Lingappa, Sivakumar;Shivakumar, Muthugounder Subramanian;Manivasagam, Thamilarasan;Somasundaram, Somasundaram Thirugnanasambandan;Seedevi, Palaniappan
    • Journal of Microbiology and Biotechnology
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    • v.31 no.6
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    • pp.867-874
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    • 2021
  • Epalrestat (EPS) is a brain penetrant aldose reductase inhibitor, an approved drug currently used for the treatment of diabetic neuropathy. At near-plasma concentration, EPS induces glutathione biosynthesis, which in turn reduces oxidative stress in the neuronal cells. In this study, we found that EPS reduces neurodegeneration by inhibiting reactive oxygen species (ROS)-induced oxidative injury, mitochondrial membrane damage, apoptosis and tauopathy. EPS treatment up to 50 µM did not show any toxic effect on SH-SY5Y cell line (neuroblastoma cells). However, we observed toxic effect at a concentration of 100 µM and above. At 50 µM concentration, EPS showed better antioxidant activity against H2O2 (100 µM)-induced cytotoxicity, ROS formation and mitochondrial membrane damage in retinoic acid-differentiated SH-SY5Y cell line. Furthermore, our study revealed that 50 µM of EPS concentration reduced the glycogen synthase kinase-3 β (GSK3-β) expression and total tau protein level in H2O2 (100 µM)-treated cells. Findings from this study confirms the therapeutic efficacy of EPS on regulating Alzheimer's disease (AD) by regulating GSK3-β and total tau proteins phosphorylation, which helped to restore the cellular viability. This process could also reduce toxic fibrillary tangle formation and disease progression of AD. Therefore, it is our view that an optimal concentration of EPS therapy could decrease AD pathology by reducing tau phosphorylation through regulating the expression level of GSK3-β.