• Journal of Plant Biotechnology
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• v.47 no.2
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• pp.118-123
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• 2020

The black seed coat of soybeans contain anthocyanins which promote health. However, mature soybean seeds contain anti-nutritional factors like lipoxygenase, lectin and Kunitz Trypsin Inhibitor (KTI) proteins. Furthermore, these seeds can be used only after the genetic elimination of these proteins. Therefore, the objective of this study was to develop novel soybean genotypes with black seed coat and triple recessive alleles (lx1lx1lx2lx2lx3lx3, titilele) for lipoxygenase, lectin, and KTI proteins. From a cross of parent1 (lx1lx2lx3/lx1lx2lx3, ti/ti, Le/Le) and parent2 (lx1lx2lx3/lx1lx2lx3, Ti/Ti, le/le), 132 F₂ seeds were obtained. A 3:1 segregation ratio was observed during F₂ seed generation for the inheritance of lectin and KTI proteins. Between a cross of the Le and Ti genes, the observed independent inheritance ratio in the F₂ seed generation was 9: 3 : 3 : 1 (69 Le_Ti_: 32 leleTi_: 22 Le_titi: 9 leletiti) (χ²=2.87, P=0.5 - 0.1). From nine F₂ seeds with triple recessive alleles (lx1lx1lx2lx2lx3lx3, titilele genotype), one novel strain posessing black seed coat, and free of lipoxygenase, lectin and KTI proteins, was selected. The seed coat color of the new strain was black and the cotyledon color of the mature seed was green. The weight of 100 seeds belonging to the new strain was 35.4 g. This black soybean strain with lx1lx1lx2lx2lx3lx3, titilele genotype is a novel strain free of lipoxygenase, lectin, and KTI proteins.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.47 no.2
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• pp.123-126
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• 2002

Lipoxygenase might be associated with seed deterioration by catalyzing the incorporation of molecular oxygen into fatty acids and generating free radicals. This study was performed to determine whether seed lipoxygenase activity would alter soybean seed longevity. In this study, germination percentage of lipoxygenase-lacking cultivar Jinpumkong2 (lx1lx1lx2lx2lx3lx3) was lower than that of Taekwangkong (Lx1Lx1Lx2Lx2Lx3Lx3). Segregation ratio for the three lipoxygenase isozymes of the F2-derived from the cross between Taekwangkong and Jinpumkong2 was fitted to 9 (Lx1Lx2Lx3) : 3 (Lx1Lx2lx3) : 3 (lxllx2Lx3) : 1 (lx1lx2lx3), suggesting the tight linkage between the Lx1 and Lx2 loci. Germination percentages varied widely but not differed among lipoxygenase isozyme types of F$_3$ seeds before and after accelerated aging. Seed coat of Jinpumkong2 was damaged severely following accelerated aging, whereas that of Taekwangkong was not. Thus, seed of lipoxygenase-lacking soybean cultivar, Jinpumkong2 showed greater deterioration compared with that of the normal Taekwangkong. However, the presence or absence of lipoxygenase activity had no effect on soybean germination.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.33 no.4
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• pp.352-359
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• 1997

The visual acuity of filefish Stephanolepis cirrhifer was studied through a series of experiments by observing their responses to target plates. The fish were trained to respond to the target plates made of white acrylic resin with a vertical black line 5cm long in the center. The width of the black line ranged from 0.2mm to 8.0 mm. The line width was diminished unto the fish could no longer distinguish the line at a distance of 100 cm from the target plate. This was repeated under light intensities of 400, 20, 5, 3 and 1 lx at the water surface. Fish were rewarded with bait in front of the target plate if the fish went to the target date (i.e., success).The results show that the training effect of 1lensh had a success rate of over 80% and that the reach times to the target plates were 4~5 seconds over 210 experimental tunes. The success rate was high using the thick line with strong apparent contrast, but was low at the 1 lx. The visible critical width of line became thick with decreasing light intensity, 0.24mm at 400 lx, followed by 0.30mm at 20 lx, 0.40mm at 5 lx, 0.46 mm at 3 lx and 2.87mm at 1 lx. The apparent contrast for visible critical width of line increased with decreasing light intensity, 0.01 at 400 and 20 lx, 0.02 at 5 lx, 0.03 at 3 lx and 0.09 at 1 lx. The line acuity of filefish was best 1.21 at the 400 lx, followed by 0.97 at 20 lx, 0.73 at 5 lx, 0.63 at 3 lx and sharply decreased to 0.10 at 1 lx. The visible ranges for 1mm and 6mm in width of line were about 4.2 m and 25.0 m at the 400 lx light intensity and decreased m 1/14 times and 1/12 times of the 400 lx at 1 lx, respectively.

• The Proceedings of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.10 no.6
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• pp.48-53
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• 1996

In this paper, the illuminance of elementary, junior, and senior high school in Inchon was measured and analyzed to investigate their night illuminating circumstances(classrooms, science rooms, and laboratories). More than four schools were selected based on the years elapsed(l0, 20, and 30 years, etc) since the opening of the schools. It was shown that the average illuminance for the classroom and that for science room and laboratory have been improved significantly such that the former was 235.6[lx] (260[lx] for senior high school, 208[lx] for junior high school, 203[lx]) for elementary school) and the latter was 233.7[lx](248.8[lx] for senior high school, 216.4[lx] for junior high school, 207.8[lx] for elementary school). The uniformity of the illuminance was exceedingly worse than the recommended one(the average value for the classroom and that for sciense room and laboratory were 0.95 and 1.08, respectively). It was found that the maximum to minimum illuminance ratio with respect to illumination derivative(7.9 for classroom, 6.8 for scienceroom or laboratory) was very high for most school buildings and was much higher for the old ones. From the measured results, it was shown that the illuminance for the school buildings needs to be improved with regard to quality.

• Korean Journal of Plant Tissue Culture
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• v.22 no.2
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• pp.71-76
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• 1995

The effects of light on the activities of several antioxidative enzymes, catalase (CAT), superoxide dismutase(SOD), ascorbate oxidase(AO), and peroxidase(POD) were examined in the hairy root cultures of Phytolacca esculenta van Houtte induced by Agrobacterium tumefaciens $A_4$T. Activities of CAT, SOD, and AO were significantly decreased with incresing light intensity (500-2,000 lx). The activity of AO under high light condition (2,000 lx)was decreased by 92% compared to the dark condition. The activities of glutathoine peroxidase (GPO), ascorbate peroxidase (APO) and general POD were increased under lower light intensify below 500 lx. The activity of GPO under 2,000 lx was decreased by 85% compared to the dark condition. The activities of antioxidative enzymes were more decreased in blue light (400-500nm). The activities of antioxidative enzymes in blue light intensity were increased in lower light intensity below 30 lx, but decreased 21-70% under 200 lx. The activity of AO was decreased by 70% under 200 lx with increasing blue light intensity. Our results suggest that the activities of antioxidative enzymes in hairy roots might be inhibited by endogenous oxidants generated under the high blue light conditions.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.3
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• pp.304-311
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• 2020

The purpose of this study is to change plant growth according to the light quantity of LED light source and to standardize plant factories technology according to the optimal LED light range. Growth was relatively stable at the 1,400 lx and 1,600 lx intensities, and growth continued with increasing light intensity, but growth slowed down with growth without leaves (Ed- I don't understand). The growth characteristics at 400 lx to 800 lx were sustained to some extent during the experimental period, but the growth of the larvae was maintained until the 7th day and the growth of leaves was not developed. From 1,000 lx to 1,400 lx, brightness, leaf growth and daily growth increased, and the growth cycle exhibited a repeating cycle of growth and slowdown. In addition, 1,600 lx and 1,800 lx showed similar growth to that at 1,400 lx, but leaf growth was high. However, at 1,800 lx, the growth was slightly higher than at 1,400lx and it slowed down over time. In addition, the stronger the light, the higher the temperature around the plant and the greater the dryness of the leaves due to the heat emitted from the light source.

• Journal of Cadastre & Land InformatiX
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• v.53 no.1
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• pp.123-137
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• 2023

This study is to review the concept of digital twin, analyse digital twin cases and suggest implications. The definition of digital twin is somewhat different depending on purposes but the importance of visualization, monitoring, analysis, prediction and optimization characteristics are accepted. In terms of the concept, digital twin cases are reviewed and analyzed. Based on the analysis, implications from a data point of view for the future development of digital twin in South Korea are suggested.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.47 no.3
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• pp.196-200
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• 2002

Lower field germination was observed in lipoxygenase- lacking soybean genotypes. This study was performed to understand seed-related traits after accelerated aging in response to planting date of soybeans. Two soybean cultivars, Jinpumkong (null lipoxygenase-2,3) and Jinpumkong 2 (lacking lipoxygenase-1, 2, 3) were evaluated for germination, and were compared with the cultivar, Taekwangkong containing lipoxygenase-1,2,3 isozymes. Greater seed coat cracking was shown in Jinpumkong and Jinpumkong 2 than Taekwangkong. Regardless of soybean genotypes, earlier planting resulted in greater seed coat cracking. After accelerated aging, seed fracturability and hardness of Jinpumkong and Jinpumkong 2 were lower than those of Taekwangkong. There was significant difference in germination percentage among soybean genotypes in response to planting date. Seeds obtained from late planting showed better germination ability. Accelerated aging resulted in lower germination percentages of Jinpumkong and Jinpumkong 2 than that of Taekwangkong, and showed higher electric conductivity in Jinpumkong and Jinpumkong 2 than Taekwangkong. After accelerated aging at 4$0^{\circ}C$ for thirty five days, germination percentages of Jinpumkong and Jinpumkong 2 were lower than that of Taekwangkong. Electric conductivity was increased continually as accelerated aging time became longer, and Jinpumkong and Jinpumkong 2 showed higher electric conductivity than Taekwangkong. Even though there were significant genotype differences in seed traits, further studies are needed to determine whether seed lipoxygenase ability is associated with germination ability.

• Journal of agriculture & life science
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• v.44 no.5
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• pp.29-33
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• 2010

Soybean (Glycine max (L.) Merr.) seed is the main source of protein and oil for human and animal. The use of soybean protein has been expanded in the food industry due to their excellent nutritional benefits. But, antinutritional and allergenic factors are exist in the raw mature soybean. Lipoxygenase, Kunitz trypsin inhibitor (KTI) protein, and ${\alpha}^{\prime}-subunit$ of 7S globulin are main antinutritional factors in soybean seed. Breeding of a new soybean strain with lacking these components is needed. The objective of this research was to select new soybean line with lipoxygenase-free, KTI-free, and ${\alpha}^{\prime}-subunit$ free (lx1lx1lx2lx2lx3lx3titicgy1cgy1 genotype). Total 434 $F_2$seeds were obtained from the cross of cultivar, "Gaechuck#2" and PI506876. Presence and absence of lipoxygenase, KTI protein, and ${\alpha}^{\prime}-subunit$ of 7S globulin was tested by SDS electrophoresis using a partial seed of each $F_2$seed. Only one $F_2$seed with lacking these three components was selected and was planted to $F_2$plant. Absence of lipoxygenase, KTI, and ${\alpha}^{\prime}-subunit$ protein was confirmed on the $F_3$seeds harvested. Selected line with lx1lx1lx2lx2lx3lx3titicgy1cgy1 genotype might be useful for soybean breeding.

• Korean Journal of Applied Biomechanics
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• v.25 no.4
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• pp.413-421
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• 2015

Objective : The purpose of this study was to investigate biomechanical changes of the lower limb including dynamic stability with changes in illumination (300Lx, 150Lx, and 5Lx) and slope (level and $15^{\circ}$ downhill) as risk factors for elderly falls. Method : Fifteen elderly females were selected for this study. Seven infrared cameras (Proreflex MCU 240: Qualisys, Sweden) and an instrumented treadmill (Bertec, USA) surrounded by illumination regulators and lights to change the levels of illumination were used to collect the data. A One-Way ANOVA with repeated measures using SPSS 12.0 was used to analyze statistical differences by the changes in illumination and slope. Statistical significance was set at ${\alpha}=.05$. Results : No differences in the joint movement of the lower limbs were found with changes in illumination (p>.05). The maximum plantar flexion movement of the ankle joints appeared to be greater at 5Lx compared to 300Lx during slope gait (p<.05). Additionally, maximum extension movement of the hip joints appeared to be greater at 5Lx and 150Lx compared to 300Lx during slope gait (p<.05). The maximum COM-COP angular velocity (direction to medial side of the body) of dynamic stability appeared to be smaller at 150Lx and 300Lx compared to 5Lx during level gait (p<.05). The minimum COM-COP angular velocity (direction to lateral side to the body) of dynamic stability appeared smaller at 150Lx compared to 5Lx during level gait (p<.05). Conclusion : In conclusion, elderly people use a stabilization strategy that reduces walk speed and dynamic stability as darkness increases. Therefore, the changes in illumination during gait induce the changes in gait mechanics which may increase the levels of biomechanical risk in elderly falls.