• Title/Summary/Keyword: LT$_{50}$

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A Cytoplasmic Polyhedrosis Virus Isolated from the Oriental Tobacco Budworm, Heliothis assulta Guenee (Lepidoptera: Noctuidae) (담배나방 세포질다각체병 바이러스의 동정 및 병원성에 관한 연구)

  • 임대준;장동숙;최귀문;강석권
    • Korean journal of applied entomology
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    • v.30 no.3
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    • pp.219-226
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    • 1991
  • A cytoplasmic polyhedrosis virus isolated from the oriental tobacco budworm, Heliothis assulta (HaCPV), was studied on morphology of the polyhedron and virus particles, analysis of viral protein and nucleic acid, and bioassay of the HaCPV to determine the feasibility of application as a microbial control agent. The shape of polyhedron was hexagonal ranging 0.5-3.7 ${\mu}m$ and the virus particles were icosahedral outline measured 55 nm in diameter. Polyhedral protein was composed of a major polypetide of 24.3 Kd and 5 minor components and virus particle had seven polypeptides ranging in 28.0 Kd-133. 6 Kd by the SDS-P AGE. The genome of virus was segmented with 10 double stranded RNA in the total mol. wt. of 18.08 Md ranging in 0.65 Md -2.79 Md. The $LC_{50}$ values of the HaCPV to the 3rd instar of H. assulta larvae were calculated to $2.895{\times}10^5PIBs/ml$. The $LT_{50}$ values in the concentration of $5.0{\times}10^{6}PIBs/ml$ was 16.4 days.

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A COMPARATIVE STUDY ON THE ACCURACY OF PREDICTION METHODS OF UNERUPTED CANINE AND PREMOLARS IN THE MIXED DENTITION (미맹출 영구치 폭경예측방법의 정확도에 관한 비교연구)

  • Lee, Eun-Young;Choi, Yeong-Chul
    • Journal of the korean academy of Pediatric Dentistry
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    • v.23 no.4
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    • pp.788-799
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    • 1996
  • The purpose of this study was to determine the accuracy of the prediction methods which are frequently used in the clinic, such as Moyers', Tanaka and Johnston's, Song's Hixon and Oldfather's, Kaplan's, Smith's. These prediction methods were compared to the data, which were obtained from 100 Kyung-Hee University students (50 males and 50 females) with normal occlusion of permanent dentition, using a retrospective statistical analysis. The standard periapical radiopgraphs for the lower premolars using 16-inch long cone technique and the yellow stone dental casts were taken from each samples. The mesiodistal widths of 4 incisors, canine, premolars were measured on the dental casts, and those of premolars were measured on the radiographs. The correlation coefficients, paired samples t-tests, regression coefficients between the actual measuredments of the samples and the predicted measurements of each methods were analyzed. The results obtained were as follows: 1. The prediction methods using periapical radiographs tended to show a stronger correlation than the methods using dental casts; the correlation coefficients between the actual widths of the lower canine and premolars and the predicted widths of the tested methods were higher in Smith, Kaplan, Hixon & Oldfater, and Tanaka & Johnston (p<0.001). 2. The Moyers' probability chart at 65 % showed a tendency toward the least difference between the actual measurements and the predicted widths of the lower canine and the premolars, and the methods of Smith, Kaplan, Tanaka & Johnston also showed a less difference. 3. By means of evaluation in correlation coefficients and paired smaples t-tests, the Smith and Tanaka & Johnston in male, and the Moyers' 65 % and Song in female showed a high tendency toward accurate methods. 4. The regression equations by models that are derived from the study were as follows: Male(Lt.) :Y=10.799+0.475X Femle(Lt.) :Y= 7.427+0.609X (Rt.) :Y=11.043+0.462X (Rt.) :Y= 8.475+0.556X 5. The regression equations by radiographs that are dericed from the study were as follows: Male(Lt.) :Y=6.5651+0.9720X Femle(Lt.) :Y=4.8656+1.0632X (Rt.) :Y=7.8610+0.9060X (Rt.) :Y=4.5483+1.0843X

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Evaluation of Susceptibility of Western Flower Thrips (Frankliniella occidentalis) and Garden Thrips (F. intonsa) to 51 Insecticides (꽃노랑총채벌레와 대만총채벌레에 대한 51종의 살충제 감수성 평가)

  • Cho, Sung Woo;Kyung, Yejin;Cho, Sun-Ran;Shin, Soeun;Jeong, Dae Hun;Kim, Sung Il;Park, Geun-Ho;Lee, Seung-Ju;Lee, Young-Su;Kim, Min-Ki;Jo, In-Jun;Koo, Hyun-Na;Kim, Hyun Kyung;Kim, Gil-Hah
    • Korean journal of applied entomology
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    • v.57 no.3
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    • pp.221-231
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    • 2018
  • The susceptibility of the western flower thrips, Frankliniella occidentalis and garden thrips, Frankliniella intonsa was evaluated using 51 commercial insecticides. 15 kinds of insecticides which showed more than 90% mortality against both thrips, F. occidentalis and F. intonsa was selected. Many active ingredients were misused and abused in commercial mixture formulation insecticides. Since the F. intonsa was more susceptible than F. occidentalis, it was considered that both thrips can be controlled by insecticides that showed insecticidal activity on the F. occidentalis. Lethal time ($LT_{50}$ and $LT_{95}$), systemic toxicity and residual toxicity of selected insecticides were compared. Both chlorpyrifos WP and chlorpyrifos + diflubenzuron WP revealed the fastest toxicity within 2 h ($LT_{95}$), while spinetoram WG revealed the slowest toxicity as 62.3 h ($LT_{95}$). Chlorfenapyr SC showed toxicity at foliar and drenching application while spinetoram WG was toxic only in foliar application. Chlorfenapyr SC showed residual effect at 3, 5, 7, 10, 15 days after treatment and both benfuracarb WG and chlorpyrifos WP showed residual effect at 3 days after treatment. As a result of treatment of selected insecticides for field population of F. occidentalis, the population collected from horticultural crops showed lower susceptibility than the population collected from vegetable crops.

Terahertz Generation and Detection Using InGaAs/InAlAs Multi Quantum Well

  • Park, Dong-U;Han, Im-Sik;No, Sam-Gyu;Ji, Yeong-Bin;O, Seung-Jae;Seo, Jin-Seok;Jeon, Tae-In;Kim, Jin-Su;Kim, Jong-Su
    • Proceedings of the Korean Vacuum Society Conference
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    • 2013.08a
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    • pp.205-205
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    • 2013
  • 테라헤르쯔(terahertz: THz)파는 0.1~10 THz 의 범위로 적외선과 방송파 사이에 광대역 주파수 스펙트럼을 차지하고 있으며 직진성, 투과성, 그리고 낮은 에너지 (meV)를 가지고 있어 비 파괴적이고 무해한 장점을 지니고 있다. Ti:sapphire laser와 같은 femto-pulse source 등이 많은 발전이 되어 현재 많은 연구와 발전이 이루어지고 있다. femto-pulse source를 이용한 THz 응용에서는 높은 저항, 큰 전자 이동도, 그리고 아주 짧은 전하수명의 기판을 요구하는데 저온에서 성장한 (low-temperature grown : LT) GaAs는 격자 내에 Gallium 자리에 Arsenic이 치환 하면서 AsGa antisite가 발생하여 전하수명을 짧아지는 것을 응용하여 가장 많이 이용되고 있다. 현재 THz 응용분야에서 보다 작고 가격경쟁력이 있는 광통신을 이용한 THz photomixer등이 활발히 연구 하고 있다. 광섬유 내에서 손실과 분산이 최소값을 가지는 부분이 1.55 ${\mu}m$ 부근이고 In0.53Ga0.47As 기판을 이용하였을 때 여기에 완벽하게 만족하게 된다. 하지만 LT-InGaAs 의 경우 AsGa antisite로 인하여 carrier lifetime은 짧아지지만 높은 n-type 전하밀도를 가지게 된다. 이때 Be을 doping하여 전하밀도를 보상하여 높은 저항을 유지해야 하는데 Be의 활성화를 위해서는 열처리를 필요로 한다. 하지만 열처리를 하면 carrier lifetime이 길어지기 때문에 carrier lifetime과 저항을 적절히 조율해야 한다. 이는 물질자체의 특성이기 때문에 InGaAs는 GaAs보다 낮은 amplitude와 짧은 cut-off frequency를 가진다. 본 연구에서는 보다 높은 저항을 얻기 위하여 molecular beam epitaxy를 이용하여 semi-insulating InP:Fe 기판위에 격자 정합된 InGaAs:Be/InAlAs multi quantum well (MQW)를 온도별 ($250{\sim}400^{\circ}C$), 주기별 (50~150)로 성장을 하였고 이때 InGaAs layer의 Be doping level은 $2{\times}1018\;cm^{-3}$, Ex-situ annealing은 $550^{\circ}C$에서 10분으로 고정 하였다. THz 발생 실험에서는 InGaAs/InAlAs MQW은 4000 pA로 1,000 pA를 가지는 InGaAs epilayer보다 4배 높은 전류 신호를 얻을 수 있었고 모든 샘플이 2 THz에서 cut-off frequency를 가지고 있었다. THz 검출 실험에서는 LT-InGaAs:Be epilayer LT-InGaAs:Be/InAlAs, HT-InGaAs/InAlAs 샘플이 각각 180, 9000, 12000 pA의 전류신호를 가지고 있었고 모든 샘플이 2 THz에서 cut-off frequency를 가지고 있었다. HT-InGaAs/InAlAs MQW를 이용한 검출실험에서는 InGaAs layer가 defect free이지만 LT-InGaAs:Be/ InAlAs MQW 보다 높은 전류 신호를 얻을 수 있었다. 이는 InAlAs layer가 저항만 높이는 것뿐만 아니라 carrier trapping layer로써의 역할도 하는 것으로 사료된다.

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Effect of Oral Administration of Seaweed-derived Fucoidan on the Resistance of the Rockfish Sebastes schlegelii to High Temperature and Streptococcus iniae Infection (해조류 유래 후코이단(Fucoidan) 경구 투여가 조피볼락(Sebastes schlegelii)의 고수온 내성 및 Streptococcus iniae 감염 저항성에 미치는 영향)

  • Song, Jun-Young;Choi, Yong-Jun;Lee, Nam-Sil;No, Chi-Won;Kim, Ahran;Do, Yong Hyun;Choi, Hye Sung
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.55 no.5
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    • pp.567-574
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    • 2022
  • Immunostimulating effects of fucoidan administration to rockfish Sebastes schlegelii at a concentration of 20 g/kg of diet were evaluated under high water temperature condition. The oral administration of fucoidan mixed with feed at a concentration of 20 g/kg of diet for 2 weeks increased the interleukin 1β gene in the intestine and kidney of fish by 5.7 and 6.3 times, respectively. In addition, when the water temperature was gradually increased from 24 to 31.4℃ for 2 weeks, LT50 delayed by 24 h in the fucoidan treated group compared to that in the control group, and mortality also reduced. Streptococcus iniae infection at a concentration of 1.50×100 CFU/fish at 28℃ delayed LT50 by 12 h in the fucoidan-treated group. Furthermore, the overall survival rate was 0% in the control group and 20% in the fucoidan-treated group. This study confirmed the applicability of dietary additives such as fucoidan as an immune activator of rockfish under high temperature condition.

Experimental Studies on the Tolerance of Anodonta woodiana against Temperature, Phenol and Lead (수온, 페놀 및 납에 대한 펄조개의 내성 실험)

  • Kim, Jae-Jin
    • The Journal of Natural Sciences
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    • v.11 no.1
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    • pp.85-88
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    • 1999
  • This study was carried out to determine the lethal water temperature and lethal concentrations of phnol and lead on Anodonta woodiana collected from Bam islet in Han river, Seoul, Korea. Lethal temperature$(LT_{48,50})$ on a woodiana was $37-38^{\circ}C$, and lethal concentrations$(LC_{50})$ of phenol and lead nitate were 1200 ppm and 220 ppm, respectively.

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Studies on the Nature and Pathogenicity of Nuclear Polyhedrosis Virus of the Fall Webworm, Hyphantria cunea (Drury) (흰불나방 핵다각체병바이러스의 성상과 병원성에 관한 연구)

  • Im Dae Joon;Hyun Jae Sun;Paik Woon Hah;Lim Jong Sung
    • Korean journal of applied entomology
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    • v.18 no.1 s.38
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    • pp.1-10
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    • 1979
  • An inclusion forming virus isolated from a fan webworm, Hyphantria cunea, in 1975 was identified as a nuclear polyhedrosis virus. With the virus isolated in Korea, it was considered that the virus would be one of the valuable microorganism in microbial control. In this connection, 1) the shape and size of the virus for identification, 2) susceptibility of the various instar larvae to the virus, 3) the effects of storage condition on the pathogenicity and the cross infection of the virus to the larvae of Bombyx mori were examined. The results are summarized as follows; 1. The polyhedron was tetrahedron or hexahedron of $2\mu$ in size and the rod-shaped virus particles consisting of $2\~14$ rods in a bundle were $330m{\mu}\times35m{\mu}$ in size. 2. The hexagonal nuclear polyhedra were found only in the nucleus of the midgut cells but were variable in size. 3. The $LD_{50}$ values for the various instar larvae of H. cunea were $8.377\times10^4\;PIBs/ml$ for the second, $4.974\times10^5\;PIBs/ml$ for the fifth instar larvae. The $LT_{50}values$ for $10^6\;PIBs/ml$ were 9.6 days for the second, 11.5 days for the third, 12.0 days for the fourth and 17 days for the fifth instar larvae. 4. The susceptibility of H. cunea to the nuclear polyhedrosis virus was greater in the first generation than in the second generation. 5. The effect of the storage conditions on the pathogenicity of the nuclear polyhedra was less in refrigerator $(5^{\circ}C)$ and in freezing $(-80^{\circ}C)$ than in room temperature $(18.5^{\circ}C)$, especially as air-dried polyhedra than as suspension. The pathogenicity of the polyhedra seemed to decrease by sunlight during storage as cadavers, since rather greater decrease in pathogenicity was found in sunny condition than in shady condition. 6. The effective spray concentration was $6.4\times10^7\;PIBs/ml$ in the field and its $LT_50$ values for the third and the fifth instar larvae were 4.8 days and 14.2 days, respectively. 7. No cross infections were found in the nuclear polyhedrosis virus between H. cunea and B. mori. larvae.

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LONGITUDINAL AND SEASONAL VARIATIONS OF THE ELECTRON TEMPERATURE AND DENSITY IN THE LOW_LATITUDE TOPSIDE IONOSPHERE OBSERVED BY KOMPSAT-1 (다목적 실용위성 1호로 측정한 저위도 상부 이온층의 전자 온도와 전자 밀도의 경도 및 계절별 변화)

  • Kim, Hee-jun;Park, Sun-Mie;Lee, Jae-Jin;Lee, En-sang;Min, Kyoung-Wook;Han, Won-yong;Nam, Uk-Won;Jin, Ho
    • Journal of Astronomy and Space Sciences
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    • v.19 no.2
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    • pp.123-132
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    • 2002
  • The electron density and temperature in the topside ionosphere are observed by the ionosphere Measurement Sensor (IMS) onboard the KOMPSAT-1, which has the sun-synchronous orbit of the altitude of 685 km and the orbital inclination of $98^{\circ}$ with a descending node at 22:50LT. Observations have been analyzed to determine the seasonal variations of the electron density and temperature in the low-latitude region. Only the night-time (22:50LT) behavior on magnetically quiet days (Kp < 4) has been examined. Observations show a strong longitudinal and seasonal variation. Generally, in the dip equator the density increases and the temperature decreases. In equinox the latitudinal distributions of the electron density and temperature are quite symmetric about the dip equator. However, the local maximum of the density and the local minimum of the temperature shift toward the Northern hemisphere in summer solstice but the Southern hemisphere in winter solstice. Such variations are due to the influences of field-aligned plasma transport induced by F region neutral wind. Compared with the IRI95 model, the observed electron density and temperature show significant differences from those predicted by the IRI95 model.

Survival and Physiological Response of Olive Flounder, Paralichthys olivaceus Exposed to Seawater Chlorinated by Chlorine Dioxide ($CIO_2$) (이산화염소($CIO_2$) 처리해수에 노출된 넙치, Paralichthys olivaceus의 생존과 생리적 반응)

  • 김흥윤;김진도
    • Journal of Aquaculture
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    • v.16 no.3
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    • pp.151-158
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    • 2003
  • This study was conducted to estimate the effects of residual chlorine dioxide ($CIO_2$) in chlorinated seawater on survival and physiological responses (hematocrit: Ht, hemoglobin: Hb, serum electrolyte and glucose levels, and osmolality) of olive flounder, Paralichthys olivaceus. All the flounders were exposed for 10 min to different concentrations of residual $CIO_2$ just after chlorination for 1 min in each experimental aquarium (EA). ClO$_2$-free seawater was continuously supplied to each EA after 10 min exposure to $CIO_2$. By means of probit analysis, the median lethal times (LT$_{50}$, min) of flounder exposed to 0.43 and 0.51 ppm $CIO_2$, were at 103 and 32 min, respectively. In the range from 0.34 to 0.51 ppm $CIO_2$, the values of Ht, Hb, electrolytes and osmolality of fish exposed to $CIO_2$ were significantly elevated as elapsed time and residual concentrations of $CIO_2$ increased after chlorination. The levels of Ht, Hb, electrolytes and osmolality in flounder exposed to 0.27 ppm $CIO_2$ were not significantly different compared to control fish, which were maintained in $CIO_2$-free seawater; however, the levels of serum glucose were significantly increased with elapse times.

Balloon Bronchoplasty for the Treatment of Bronchial Stenosis After Lung Transplantation: A Single-Center 10-Year Experience

  • Dong Kyu Kim;Joon Ho Kwon;Kichang Han;Man-Deuk Kim;Gyoung Min Kim;Sungmo Moon;Juil Park;Jong Yun Won;Hyung Cheol Kim;Sei Hyun Chun;Seung Myeon Choi
    • Korean Journal of Radiology
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    • v.24 no.5
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    • pp.424-433
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    • 2023
  • Objective: To assess the safety and efficacy of balloon dilatation under dual guidance using fluoroscopy and bronchoscopy for treating bronchial stenosis following lung transplantation (LT), and to elucidate the factors associated with patency after the procedure. Materials and Methods: From September, 2012, to April, 2021, 50 patients (mean age ± standard deviation, 54.4 ± 12.2 years) with bronchial stenosis among 361 recipients of LT were retrospectively analyzed. The safety of balloon dilatation was assessed by evaluating procedure-related complications. Efficacy was assessed by evaluating the technical success, primary patency, and secondary patency. Primary and secondary cumulative patency rates were calculated using the Kaplan-Meier method. The factors associated with patency after the procedure were evaluated using multivariable Cox hazard proportional regression analysis. Results: In total, 65 bronchi were treated with balloon dilatation in 50 patients. The total number of treatment sessions was 277 and the technical success rate was 99.3% (275/277 sessions). No major procedure-related complications were noted. During the mean follow-up period of 34.6 ± 30.8 months, primary patency was achieved in 12 of 65 bronchi (18.5%). However, the patency rate improved to 76.9% (50 of 65 bronchi) after repeated balloon dilatation (secondary patency). The 6-month, 1-year, 3-year, and 5-year secondary patency rates were 95.4%, 90.8%, 83.1%, and 78.5%, respectively. The presence of clinical symptoms was a significant prognostic factor associated with reduced primary patency (adjusted hazard ratio [HR], 0.465; 95% confidence interval [CI], 0.220-0.987). Early-stage treatment ≤ 6 months (adjusted HR, 3.588; 95% CI, 1.093-11.780) and prolonged balloon dilatation > 5 min (adjusted HR, 3.285; 95% CI, 1.018-10.598) were associated with significantly higher secondary patency. Conclusion: Repeated balloon dilatation was determined to be safe and effective for treating bronchial stenosis following LT. Early-stage treatment and prolonged balloon dilatation could significantly promote long-term patency.