• Title/Summary/Keyword: LT$_{50}$

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Fumigant Toxicity of Essential Oils against Adults of Culex pipiens pallens (빨간집모기 성충(Culex pipiens pallens)에 대한 식물오일의 훈증효과)

  • Kang, Shin-Ho;Kim, Min-Ki;Seo, Dong-Kyu;Kim, Gil-Hah
    • The Korean Journal of Pesticide Science
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    • v.10 no.2
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    • pp.117-123
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    • 2006
  • Fumigant toxicity of 34 plant essential oils were tested against female adults of Culex pipiens pallens. Seven oils (basil, coriander, fennel, pennyroyal, peppermint, thyme red, thyme white) showed more than 60% mortality at 1 ${\mu}L\;L^{-1}$ air concentration and pennyroyal oil appeared to have the highest adulticidal activity. GC and GC-MS analysis of the seven essential oils and bioassay of their components revealed that pulegone (a major component of pennyroyal oil) and anethole (a major component of fennel and basil oil, $LD_{50}$, ${\mu}L\;L^{-1}$ air =0.49) showed higher adulticidal activities than others. In addition to the above two components, linalool ($LT_{50}$, min=0.91), camphor ($LT_{50}$, min=1.06), fenchone ($LT_{50}$, min=1.16), 1,8-cineole ($LT_{50}$, min=2.93), and geranyl acetate ($LT_{50}$, min=1.60) also showed higher adulticidal activity than empenthrin ($LT_{50}$, min=3.45).

Pathogenicity of Spodoptera litura Nucleopolyhedrovirus with Different Temperatures (온도조건에 따른 담배거세미나방핵다각체병바이러스의 병원성 검정)

  • 김선곤;김도익;박종대;최형국;유용만
    • Korean journal of applied entomology
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    • v.42 no.2
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    • pp.159-163
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    • 2003
  • This experiment was conducted to investigate pathogenicity of Spodoptera litura nucleopolyhedrovirus (SINPV) with different temperatures to mass production. In laboratory, LC$_{50}$ values of SINPV were 5.534$\times$10$^3$ PIBs/ml and 4.l0$\times$10$^2$PIBs/ml in 1st instar larvae at 2$0^{\circ}C$ and 28$^{\circ}C$, respectively, but those were increased at 32$^{\circ}C$. LC$_{50}$ at 3rd and 5th instar larvae were showed the lowest value at 28$^{\circ}C$. LT$_{50}$ values of SINPV in 1.0$\times$10$^{5.7}$ PIBs/ml were determined at various temperature conditions (20-32$^{\circ}C$). The result showed that treatments at 28$^{\circ}C$ and 32$^{\circ}C$ shortened LT$_{50}$ values, but treatments at 2$0^{\circ}C$ and 24$^{\circ}C$ was relatively longer. Also LT$_{50}$ values was shortened in high concentration and young larva. LT$_{50}$ values are dependent on the temperature, viral concentration and larval instar.

Eco-frendly Control of Culex pipiens (mosquito) Larvae by Acorus calamus (sweet flag) and Acorus gramineus (Grassy-leaved sweet flag) Extracts

  • Choi, Jeong-Keun;Lee, Ji-Yeon;Lee, Ja-Hyun;Lee, Seong-Gene;Han, Yeon-Soo;Han, Tae-Ho
    • Korean Journal of Plant Resources
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    • v.19 no.6
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    • pp.721-726
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    • 2006
  • Mosquitoes are carriers of malaria and encephalitis. This study performed for eco-friendly control of mosquitos by using genus Acorus. Several solvents were used for the extraction of genus Acorus; water, ethanol, and methanol. Grinded leaves and roots were also included. Acorus extracts killed mosquito larvae and the ethanol extract showed the best result. Autoclaved Acorus water needed long time to kill mosquito larvae. $LT_{50}$ of 1 % Acorus calamus decoction was 13.6 hrs and 1 % autoclaved Acorus water was 53.6 hrs. $LT_{50}$ of 0.05% Acorus calamus rhizome powder was 28.5 hrs. $LT_{50}$ of 0.5% Acorus calamus leaf powder was 10.8 hrs. $LT_{50}$ of 0.1 % Acorus calamus decoction was 63.4 hrs and 0.1 % Acorus calamus ethanol extracts was 48.6 hrs and 0.1% Acorus calamus methanol extracts was 53.9 hrs. $LT_{50}$ of 0.4% Acorus gramineus decoction was 45.5 hrs, 0.4% ethanol extracts was 10.9 hrs, 0.4% methanol extracts was 10.2 hrs. $LT_{50}$ of ethanol extracts was shorter than other extracts. Acorus calamus rhizome powder could be used for the eco-friendly control of the mosquito larvae.

Estimating Utilization Factor of Left Turn Lane for Through Traffic, Intersection Capacity, and Optimum Signal Timings (직진교통의 좌회전차선 이용률 추정과 교차로용량 및 최적신호등시간 산정)

  • 도철웅
    • Journal of Korean Society of Transportation
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    • v.1 no.1
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    • pp.56-63
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    • 1983
  • Intersection control has dual-purposes; increasing capacity and reducing delay. The primary concern of efficient intersection control under oversaturated condition as in Korea is to increase capacity. Prevailing intersection operation technique permits thru traffic to utilize left turn lane, because the intersection without left turn pocket has left turn signal interval. In this situation, it seems not to be valid to calculate capacity, delay, and signal timings by conventional methods. By critical lane technique, capacity increases as cycle length increases. However, when thru traffic utilize LT lane, the capacity varies according to LT volume, LT interval as well as cycle length, which implies that specific cycle length and LT interval exist to maximize capacity for given LT volume. The study is designed is designed to calculate utilization factors of LT lane for thru traffic and capacities, and identify signal timings to yield maximum capacity. The experimental design involved has 3 variables; 1)LT volumes at each approach(20-300 vph), 2)cycle lengths (60-220 sec), and 3)LT intervals(2.6-42 sec) for one scenario of isolated intersection crossing two 6-lanes streets. For LT volume of 50-150 vph, capacity calculated by using the utilization factor is about 25% higher than that by critical lane method. The range of optimum cycle length to yield maximum capapcity for LT volume less than 120 vph is 140-180 sec, and increases as LT volume increases. The optimum LT interval to yield maximum capacity is longer than the intrval necessary to accommodate LT volume at saturation flow rate.

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Hatching Rate and Larval Viability of Cultured Marine Fish Exposed to Water-soluble Fraction of Kuwait Crude Oil during Egg Development (난발생중 원유의 수용성 성분에 노출된 해산 양식어류 수정란의 부화율 및 자어의 생존능력)

  • 이갑현;장영진;강덕영
    • Journal of Aquaculture
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    • v.12 no.2
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    • pp.115-122
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    • 1999
  • Exposure experiments during the egg development were conducted to assess the influences of 5 different concentrations (0, 25, 50, 75 and 100%) of water-soluble fraction (WSF) of Kuwait crude oil on the eggs and larvae of black seabream (Acanthopagrus schlegeli), red seabream (Pagrus major) and olive flounder (Paralichthys olivaceus). All experiments were triplicated. Hatching time and hatching rate were examined on the eggs. The median lethal time ($LT_{50}$), morphological abnormality and swimming activity (swimming frequency and speed) of larvae were also investigated. The time and rate of egg hatching were not significantly influenced by WSF on the eggs of the fishes. The larvae exposed to WSF during the egg development were also not significantly influenced on the $LT_{50}$ and swimming activity. But the higher morphological abnormalities of notochord were observed from the larvae in 100% WSF exposure.

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Comparison of Sensitivity of Detection for Enterotoxigenic Escherichia coli Enterotoxin and Clostridium perfringens Type A Enterotoxin by Means of the Reversed Passive Latex Agglutination and the Polymerase Chain Reaction (독소원성 대장균과 Clostridium perfringens A형이 생산하는 장독소의 검색을 위한 RPLA 법과 PCR기법의 감도 비교)

  • 정희곤
    • Journal of the East Asian Society of Dietary Life
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    • v.11 no.1
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    • pp.26-32
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    • 2001
  • 독소원성 대장균(enterotoxigenic Escherichia coli, ETEC, EC81, serotype O148:H28)이 생산하는 heat labile enterotoxin(LT)를 검색해 본 결과, reversed passive latex agglutination(RPLA)법에 있어서는 2배로 희석한 용액 (50 ng)으로부터 64로 희석한 용액 (1.56 ng)에서까지 양성반응을 보였으며 polymerase chain reaction (PCR)기법에 있어서는 10 ng으로부터 1 pg희석용액에서까지 147-base pair(bp)의 LT DNA fragment가 확인되었다. Clostridium perfringens A형 (NCTC8238, Hobbs serotype 2)이 생산한는 장독소를 검색해 본 결과, RPLA법에 있어서는 2배로 희석한 용액 (50 ng)으로부터 64로 희석한 용액 (1.56 ng)에서 까지 양성반응을 보여 독소원성대장균이 생산하는 LT와 일치하였으나, PCR기법에 있어서는 10ng로부터 10 pg 희석용액에서 까지 354-bp의 DNA fragment가 확인되어 독소원성대장균이 생산하는 LT보다 1/10의 낮은 감도를 보였다. PCR기법은 RPLA법에 비하여 훨씬 신속하고 소량의 sample로 장독소를 확인할 수 있었다.

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Enhanced Thermal Stability of a Novel Human Thrombopoietin Mutein under the Various Temperature Conditions

  • Kim, Tae-Soo;Ahn, Hye-Kyung;Lim, Seung-Wook;Hong, Yeon-Joo;Chung, Joo-Young;Koh, Yeo-Wook
    • Proceedings of the PSK Conference
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    • 2003.10b
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    • pp.237.2-237.2
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    • 2003
  • DWP40458 is a novel human thrombopoietin mutein with two additional N-linked glycosylation site. The thermal stability of DWP40458 in both solution and lyophilized form was studied in the temperature range of 4-50$^{\circ}C$, compared with recombinant human TPO (rhTPO). When the aggregation or degradation pattern of DWP40458 and rhTPO solution was characterized by using SDS-PAGE, gel permeation chromatography (GPC) and reverse phase HPLC, it was found that thermostability of DWP40458 was significantly different to rhTPO in the temperature at 25, 40, 40, 50$^{\circ}C$. (omitted)

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Effects of Different Temperatures on Pathogenicity of Spodoptera exigua Nucleopolyhedrovirus (SeNPV) (온도조건에 따른 파밤나방핵다각체병바이러스(SeNPV)의 병원 활성)

  • Kim Seon-Gon;Park Jong-Dae;Kim Do-Ik;Choi Hyeong-Gug;Kim Sang-Soo;Hwang In-Cheon
    • Korean journal of applied entomology
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    • v.43 no.4 s.137
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    • pp.329-332
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    • 2004
  • This experiment was conducted to investigate pathogenicity of Spodoptera exigua nucleopolyhedrovirus (SeNPV) with different temperatures for mass production. In laboratory condition, $LC_{50}$ values of SeNPV were $9.797{\times}10^{3}PIBs/mL$ at $20^{\circ}C$ and $3.351{\times}10^{2}PIBs/mL$ at $32^{\circ}C$ in 2nd instar larvae. $LC_{50}$ of the other larval stage were similar to that of 2nd instar. $LT_{50}$ values of SeNPV was 9.0 days in $1.0{\times}10^{3}PIBs/mL$ but 6.9 to 3.5 days in $1.0{\times}10^{4-6}PIBs/mL$ against 3rd instar of Spodoptera exigua. $LT_{50}$ Values of $1.0{\times}10^{4}PIBs/mL$ were 5.7, 5.5 and 4.9 days in 24, 28 and $32^{\circ}C$, respectively. As a results, $LT_{50}$ was shortened with increase of temperatures up to $32^{\circ}C$ and also dependent on viral concentration and larval instars.

Acute Toxicity of Synthetic Detergent on the Larvae of Loach, Misgurnus angillicaudatus (미꾸리 자어에 대한 합성세제의 급성독성)

  • LEE Jeong Yeol;CHIN Pyung
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.17 no.2
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    • pp.139-142
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    • 1984
  • Short-term acute toxicity of synthetic detergent(LAS) to larvae of loach, Misgurnus angillicaudatus was examined by static bioassay. The larvae were exposed to 15 different concentration of synthetic detergent for 16, 48, 72, 96 and 120 hours in order to determine median lethal concentration($LC_{50}$). The $100\%$ mortarlity of larvae was showed within 120, 96, 48 and 16 hours for 6, 18, 30 and 38 ppm, respectively. The median lethal concentration values of the larvae were 12.59 ppm for 48 hours, 4.00 ppm for 96 hours and 1.02 ppm for 120 hours. The permissible toxicant concentration of acute toxicity to larvae was $0.37{\sim}0.43$ ppm, and application factor of the synthetec detergent was $0.093{\sim}0.108$. The median lethal time($LT_{50}$) for different concentration also was determined. The $LT_{50}$ of 0.2 ppm was found within 165.1 hours and 2 ppm was 106.2 hours, while the $LT_{50}$ of 8 ppm was 60.3 hours and that of 38 ppm was 23.5 hours.

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On-the-Fly Belief Propagation Decoding of Decentralized Luby Transform Codes for Distributed Storage in a Small-Scale Wireless Sensor Network (소규모 무선 센서 네트워크에서 분산 저장을 위한 LT 부호의 OBP 복호)

  • Cheong, Ho-Young
    • The Journal of Korea Institute of Information, Electronics, and Communication Technology
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    • v.9 no.5
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    • pp.503-510
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    • 2016
  • In this paper I analyzed two decoding algorithms of decentralized LT codes for distributed storage by simulations in small-scale wireless sensor network. From the simulation results we can see that when the decoding ration is above 2.0, the successful decoding probability of OBP decoding is about 99%, while that of BP decoding is below 50% with n=100, and about 70% with n=200. We showed that OBP decoding algorithm is an efficient decoding scheme for decentralized LT codes for distributed storage in small-scale wireless sensor network.