• BMB Reports
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• 제54권10호
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• pp.522-527
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• 2021

Lysine-specific demethylase 1 (LSD1) is an epigenetic regulator that modulates the chromatin status, contributing to gene activation or repression. The post-translational modification of LSD1 is critical for the regulation of many of its biological processes. Phosphorylation of serine 112 of LSD1 by protein kinase C alpha (PKCα) is crucial for regulating inflammation, but its physiological significance is not fully understood. This study aimed to investigate the role of Lsd1-S112A, a phosphorylation defective mutant, in the cigarette smoke extract/LPS-induced chronic obstructive pulmonary disease (COPD) model using Lsd1^SA/SA mice and to explore the potential mechanism underpinning the development of COPD. We found that Lsd1^SA/SA mice exhibited increased susceptibility to CSE/LPS-induced COPD, including high inflammatory cell influx into the bronchoalveolar lavage fluid and airspace enlargement. Additionally, the high gene expression associated with the inflammatory response and oxidative stress was observed in cells and mice containing Lsd1-S112A. Similar results were obtained from the mouse embryonic fibroblasts exposed to a PKCα inhibitor, Go6976. Thus, the lack of LSD1 phosphorylation exacerbates CSE/LPS-induced COPD by elevating inflammation and oxidative stress.

• 한국윤활학회:학술대회논문집
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• 한국윤활학회 2000년도 제31회 춘계학술대회
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• pp.87-92
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• 2000

In order to improve the instability of Plane journal bearing, it is introduced the leaf spring damper(LSD). The effects of dynamic characteristics of LSD on the stability of a plane journal bearing were investigated theoretically. The improvements on the stability of a plane journal bearing with LSD. are compared with the results of a floating ring journal bearing and of journal bearing without LSD. It found that a plane journal bearing with LSD will be an effective way in the improvement on the stability of rotor system.

• Tribology and Lubricants
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• 제17권3호
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• pp.179-184
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• 2001

In order to improve the instability of plane journal bearing, it is introduced the leaf spring damper(LSD). The effects of dynamic characteristics of LSD on the stability of a plane Journal bearing were investigated theoretically. The improvements on the stability of a plane journal bearing with LSD are compared with the results of a floating ring journal bearing and of journal bearing without LSD. It found that a plane journal bearing with LSD will be an effective way in the improvement on the stability of rotor system.

• 한국지진공학회:학술대회논문집
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• 한국지진공학회 2000년도 추계 학술발표회 논문집 Proceedings of EESK Conference-Fall 2000
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• pp.490-495
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• 2000

This paper presents the dynamic behavior of stay cable with Lead-Shear damper( LSD) near the support. This kind of research about the dynamic behavior of LSD is essential to design LSD in order to mitigate the ambient vibration of stay cable. The hysteresis curve of LSD was assumed to be perfect elasto-plastic behavior based on the real hysteretic behavior of such lead-based dampers. Mechanical model of LSD was equivalent Kelvin model and sag effect of stay cable was considered. Yielding force (also referred as size) of LSD was selected as a design parameter. Effects of tension of stay cable and installation point of LSD were studied. It was found that optimal size of LSD exists for each case of stay cable.

• BMB Reports
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• 제53권7호
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• pp.385-390
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• 2020

Inflammatory Bowel Disease is caused by an acute or chronic dysfunction of the mucosal inflammatory system in the intestinal tract. In line with the results of our previous study, wherein we found that the PKCα-LSD1-NF-κB signaling plays a critical role in the prolonged activation of the inflammatory response, we aimed to investigate the effect of signaling on colitis in the present study. Lsd1 S112A knock-in (Lsd1^SA/SA) mice, harboring a deficiency in phosphorylation by PKCα, exhibited less severe colitis symptoms and a relatively intact colonic epithelial lining in dextran sulfate sodium (DSS)-induced colitis models. Additionally, a reduction in pro-inflammatory gene expression and immune cell recruitment into damaged colon tissues in Lsd1^SA/SA mice was observed upon DSS administration. Furthermore, LSD1 inhibition alleviated colitis symptoms and reduced colonic inflammatory responses. Both LSD1 phosphorylation and its activity jointly play a role in the progression of DSS-induced colitis. Therefore, the inhibition of LSD1 activity could potentially protect against the colonic inflammatory response.

• 생명과학회지
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• 제28권3호
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• pp.351-356
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• 2018

L-세린 탈수화효소(LSD)는 L-serine을 피루브산과 암모니아로 전환하는 반응을 촉매하는 iron-sulfur 함유 효소이다. 세균성 아미노산 탈수화 효소 중에서, L-serine에 대한 이들 특정 효소만이 촉매 부위에서 iron-sulfur cluster를 이용하는 것으로 보고되고 있다. 또한, 세균성 LSD는 구조적 특성과 도메인의 배열에 따라 네 가지 유형으로 분류된다. 현재까지, 이 효소들은 소수의 균주로부터 얻어진 LSD 효소에 대해서만 연구되었지만, 다양한 세균성 LSD의 촉매 메커니즘을 이해하기 위해서는 더 많은 자세한 조사가 요구된다. 본 연구에서, Mycobacterium tuberculosis H37Rv로부터 유래된 유형 II LSD (MtLSD) 단백질을 효소 동력학적 방법을 이용하여 생화학적 및 촉매적 특성을 규명하기 위해 발현 및 정제되었다. MtLSD에 대한 L-serine의 포화 곡선은 알로스테릭 협동성(allosteric cooperativity)을 나타내는 전형적인 S자형(sigmoid)의 특성을 보였다. 이때의 $K_m$과 $k_{cat}$ 값은 각각 $59.35{\pm}1.23mM$과 $18.12{\pm}0.20s^{-1}$로 계산되었다. 또한, 고정된 L-serine 농도 하에서 D-serine의 농도 대비 초속도에 대한 그래프는 비선형 쌍곡선 감쇠 형태를 보였고, $k_{cat}$ 값의 변화 없이 $30.46{\pm}5.93mM$의 겉보기 $K_i$ 값으로 D-serine에 대한 경쟁적 억제(competitive inhibition)를 나타내었다. 이들 연구는 MtLSD의 촉매 특성 및 기질 특이성에 관한 통찰력 있는 생화학적 정보를 제공한다.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2004년도 추계학술대회 논문집
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• pp.131-136
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• 2004

The limited slip differential(LSD) is a device which enables the driving force to be transmitted from one slipping wheel to another wheel in such case that the car is stuck in clay or snow. When the unwanted slipping occurs on one wheel, the LSD temporarily restraints the differential motion to transmit the driving force in the other wheel. So far, many types of LSD were developed such as mechanical lock type, disk clutch type, viscous coupling type, torsion type and multiple clutch type. However these types of LSD is too complicated and expensive, so it is used only for 4WD outdoor vehicles, military vehicles, and a portion of deluxe car. So, many studies has been devoted to improve new types of LSD to cover those demerits of existing LSDs that the hydraulic LSD is developed as arepresentative result of that. The hydraulic LSD which uses the principle of gear pump is packed with viscous oil in tight container. When a slip occurs on one wheel, the hydraulic LSD generates torque caused by high oil pressure in the container. This study has been devoted to suggest an improved hydraulic LSD. In order to achieve it, we designed a new type of hydraulic LSD, produced it and did a rig test with it on real vehicle. From the rig test, it has been confirmed that the new type of hydraulic LSD can be directly applied to exiting vehicles without changing the design criteria

• 한국소음진동공학회논문집
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• 제14권6호
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• pp.463-469
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• 2004

In order to improve the instability of journal bearings, the leaf spring dampers (LSD) are introduced. The effects of LSD on the stability of journal bearings are investigated theoretically The stability of the journal bearing with LSD are compared with the results of the journal bearing without LSD. And the effects of the asymmetry of the stiffness of the leaf spring damper on the stability of rotors are also investigated.

• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 2003년도 춘계학술대회논문집
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• pp.100-106
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• 2003

In order to improve the instability of a plane journal bearing, the leaf spring dampers (LSD) are introduced. The effects of LSD on the stability of a plane journal bearing are investigated theoretically. The stability of a plane journal bearing with LSD are compared with the results of a plane journal bearing without LSD. And the effects of the asymmetry of the stiffness of the leaf spring damper on the stability of rotors are also investigated.

• Journal of Veterinary Science
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• 제24권3호
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• pp.42.1-42.6
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• 2023

Lumpy skin disease (LSD) is one of the most important emerging transboundary diseases. Recently, LSD has emerged in many countries in the northern hemisphere. The LSD virus has a huge genome and is highly resistant to environmental conditions. The virus is also host-specific and large ruminants, such as cattle and domestic water buffalo, are particularly susceptible. In addition, wild ruminants can serve as potential reservoirs for spreading the LSD virus. The emergence might be related to climate change in various regions because LSD is an arthropod-borne infectious disease. This disease causes enormous economic losses, such as leather damage, decreased milk production, abortion, and death in infected ruminants. The economic importance of LSD in the bovine industry has forced countries to develop and implement control strategies against the disease. With the recent global spread and the economic impact, LSD will be discussed intensively. In addition, effective preventive measures are suggested based on the presence or absence of LSD outbreaks.