• 한국산림과학회지
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• 제99권4호
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• pp.633-637
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• 2010

본 연구는 낙엽송의 4가지 배발생조직(LL-L, LL-K, LL-P 및 LL-N)으로 체세포배 유도에 영향하는 배지종류, 엡시식산(abscisic acid, ABA)의 농도, 삼투압제의 종류 및 농도의 효과를 평가하기 위해 수행되었다. 배지종류 비교에서 1/2LM 배지에 배양한 LL-P 조직라인(134.9개/90 mg조직)에서 가장 높은 자엽단계의 체세포배가 유도되었으나 BLG 배지의 경우 LL-P라인(32.9개)을 제외하고는 체세포배가 전혀 유도되지 않았다. 2가지 ABA농도(60 혹은 100 ${\mu}M$)에 대한 4가지 배발생조직의 체세포배 유도 효과를 비교 한 바 최대 체세포배는 60 ${\mu}M$ ABA농도(142.9개)에서 나타났으나 LL-N 라인을 제외하고는 체세포배 발생에 ABA영향은 그리 크지 않은 것으로 나타났다. 삼투압제 종류 및 농도 비교에서 최고의 체세포배 발생은 LL-K 조직라인을 0.2 M maltose 첨가배지(540.5개)에 배양하였을 때 나타났다. 결론적으로 체세포배 발생 효과는 배지의 종류, 혹은 배지에 첨가된 ABA 및 삼투압제 농도보다는 배발생 조직 라인에 따라 크게 좌우되었다.

• 대한한의학회지
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• 제17권2호
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• pp.227-236
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• 1996

The effect of Ligustrum Lucidum(LL) on the production of nitric oxide (NO) and superoxide by murine peritoneal macrophages were investigated. Stimulation of the cells with LL in the presence or absence of interferon-r(IFN-r) resulted in the increased accumulation of nitrite in the medium. To further examine the mechanism of LL induced. NO Synthesis, we evaluated the secretion of tumor necrosis $factor-{\alpha}(TNF-{\alpha})$ by LL in murine macrophages. Treatment of LL increased the secretion of bioactive $TNF-{\alpha}$ in cultured medium. In addition, LL induced NO production was decreased by the treatment of anti-murine $TNF-{\alpha}$. neutralizing antibodies, indicating that LL induced superoxide production was decreased by the treatment of anti-murine $TNF-{\alpha}$ neutralizing antibodies. These data suggested that LL induced superoxide production was related to $TNF-{\alpha}$ secretion. In conclusion, our results indicates that LL may enhance innate immune response and be applied as a immunoregulating drug improving phagocytosis.

• 한국식품영양학회지
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• 제12권5호
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• pp.523-528
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• 1999

The influence of ginseng on the paste or gelatinization properties by amylograph and mixing properties by farinograph of wheat flour and on quality properties color cooking quality textural and sensory properties and reducing microbial population of LL(Long Life) noodles was studied. The contents of ginseng used were from 5% to 10% based on flour weight. The viscosity property of wheat flour with ginseng was increased the initial pasting temperature but the amylograph peak viscosity were decreased in vice versa. The farinograph absorption stability and breakdown were increased by ginseng. The whiteness of Ll nodles manufactured with ginseng was lower than that of control The shear extrusion force and hardness of LL noodles manufactured with ginseng were shown much higher value than those of control. At cooking quality examination of LL noodles manufactured with ginseng weight of cooked LL noodles was decreased but volum was appeared in ice versa,. Extraction amounts of LL noodles manufactured with ginseng during cooking were much smaller than those of control Total count of microorganism of Ll noodles manufactured with ginseng were decreased during storage at 3$0^{\circ}C$ Sensory properties of cooked LL noodles which was manufactured with ginseng showed quite acceptable. Based on the cooking and sensory evaluation test addition of 7.0% ginseng to wheat flour may be suitable for processing LL noodles.

• 한국식생활문화학회지
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• 제13권3호
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• pp.191-196
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• 1998

LL면 제조에 있어서 유기산의 첨가가 LL면의 색상, 조직감, 저장 안정성과 관능적 특성에 미치는 영향을 검토하기 위하여 유기산의 종류를 달리한 다음 밀가루에 0.2%를 첨가하여 LL면을 제조한 뒤 조리시험, 면의 조직감 특성, 미생물 측정 및 관능검사를 평가하였다. Hunter L값은 dl-malic acid를 첨가하여 제조한 LL면이 70.49로 가장 높게 나타났으며, Hunter a, b값은 모든 처리구가 유사하게 측정되었다. 층밀림 압출력은 dl-malic acid를 첨가하여 제조한 처리구 B가 20.55(kgf)로 가장 높게 나타났으며. 젖산을 첨가한 처리구 A가 가장 작은 수치인 18.05(kgf)로 측정되었다. 경도도 dl-malic acid를 첨가하여 처리구 B가 25.43(kgf)으로 가장 높게 나타났으며 젖산을 첨가한 처리구 A는 21.21(kgf)으로 측정되었으나 처리구간에 큰 차이는 없었다. dl-malic acid를 첨가한 처리구 B와 dl-사과산과 초산을 1:1로 첨가한 처리구 D를 첨가하여 제조한 LL면의 무게 증가는 다른 처리구보다 낮게 나타났다. 반면에 부피 증가는 오히려 높게 나타나 조직이 다소 치밀함을 알 수 있었다. 용출양의 변화는 모든 처리구가 $16{\sim}25%$의 범위를 나타냈으며 dl-malic acid를 첨가한 처리구 B와 dl-사과산과 초산을 1:1로 첨가한 처리구 D를 첨가하여 제조한 LL면이 감소하는 경향을 보였다. 미생물수는 dl-malic acid를 첨가한 처리구 B와 dl-사과산과 초산을 1:1로 첨가한 처리구 D를 첨가하여 제조한 L면이 $30^{\circ}C$에서 5개월 이상 저장에도 검출되지 않았으나 control를 포함하여 젖산을 첨가한 처리구 A와 초산을 첨가한 처리구 C는 저장 1개월 이후부터 증가하는 경향을 보였다. 관능검사 결과는 dl-malic acid를 첨가한 처리구 B와 dl-사과산과 초산을 1:1로 첨가한 처리구 D를 첨가하여 제조한 LL면이 4.3 및 4.0으로 젖산을 첨가한 처리구 A와 초산을 첨가한 처리구 C의 3.1, 3.2보다 좋은 점수를 얻었다.

• Journal of Acupuncture Research
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• 제18권6호
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• pp.250-263
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• 2001

Objective : I refered to oriental medical records to study on the use Ohaeng-acupuncture through compared ${\ll}$Classic on Difficulty${\gg}$ with . Methods : The original text about ${\ll}$Classic on Difficulty${\gg}$ was used ${\ll}$Nan Jing Ben YI${\gg}$, annotations were excerpted and record that were necessary for this study. The structural formula was composed together to compare ${\ll}$Classic on Difficulty${\gg}$ with . Results : ${\ll}$Classic on Difficulty${\gg}$ deals with fundamental medical theories and gives differentiation of syndromes of some diseases in the form of questions and answers. ${\ll}$The Sixty nineth Difficulty, Classic on Difficulty${\gg}$ 'Xu Ze Bu Qi Mu(虛者補其母), Shi Ze Xie Qi Zi (實者瀉其子)' that united ${\ll}$Ling Shu(靈樞) - Jing Mai(經脈篇)${\gg}$ 'Sheng Ze Xie Zhi(盛則瀉之) Xu Ze Bu Zhi(虛則補之)' with Ohaeng-xiangsheng theory is the base of the 'Bu Xie (補瀉)'. ${\ll}$The seventy fifth Difficulty, Classic on Difficulty${\gg}$ 'Xie Nan Huo (瀉南方火) Bu Bei Shui (補北方火)' that based Ohaeng-xiangke theory and the 'Qu Xue(取穴)' takes the form of the 'Bu Mu Xie Zi (補母瀉子)' in standard of internal organs which are etiologic al cause named 'Shi(實)'.

• 한국축산식품학회지
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• 제39권5호
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• pp.792-803
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• 2019

The supplementation with natural ingredients that are rich in polyphenols could improve the quality and functionality of yogurt. Lotus leaf (LL) are abundant in phenolic compounds. We aimed to investigate the effects of LL powder on the quality properties, total phenolic content (TPC), and antioxidant activity of yogurt. Yogurt was supplemented with four different concentrations (0%, 0.2%, 0.5%, and 1%) of LL powder and evaluated for 14 d refrigerated storage. During storage, the titratable acidity (TA) of all LL yogurt groups was higher than that of the control (p<0.05). The $L^*$ and $a^*$ values decreased by LL addition to the yogurts, while the $b^*$ values increased. All LL yogurt groups indicated better viscosity than that of control, and among them, 0.2% LL yogurts had the highest viscosity without significant decrease until the end of the storage duration. The addition of LL into yogurt enhanced the water-holding capacity (WHC) by at least 1.5-fold than that of control for the entire storage duration. The TPC of yogurts gradually increased with the addition of LL (p<0.05) and continued to increase during storage; the 1% LL yogurt at 14 d showed the highest value of $61.94{\pm}1.68{\mu}g$ GAE/g. The DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity and reducing power of the yogurts were also significantly enhanced with increasing LL concentrations (p<0.05). These results suggest that lotus leaf may be useful as a natural ingredient for improving the quality and antioxidant activity of yogurt.

• 한국수학교육학회지시리즈A:수학교육
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• 제53권1호
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• pp.111-129
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• 2014

${\ll}$Xiang Ming Suan Fa${\gg}$ written by Ahn Ji-Jae, a scholar of Yuan Dynasty, is a very important mathematics text in development of mathematics in Joseon Dynasty. Also, ${\ll}$Xiang Ming Suan Fa${\gg}$ in possession of Keimeung university was designated as a Korean National Treasure on February 25, 2011. In this paper, we analyzed the structure and contents of ${\ll}$Xiang Ming Suan Fa${\gg}$. Also, we studied the influences of ${\ll}$Xiang Ming Suan Fa${\gg}$ on Joseon Dynasty's mathematics according to the comparing with mathematics books such as ${\ll}$Mook Sa Jib San Bub> and ${\ll}$San Hak Yib Moon${\gg}$.

• Journal of Acupuncture Research
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• 제19권4호
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• pp.124-131
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• 2002

Objective : The purpose of this study is to research for the formation of Ohaeng-acupuncture. Methods : I refered to ${\ll}$Classic on Difficulty${\gg}$ (難經), ${\ll}$Ling Shu${\gg}$ (靈樞), ${\ll}$Zhen Jiu Ju Ying${\gg}$ (針灸聚英), ${\ll}$Yi Xue Ru Men${\gg}$ (醫學入門) and annotations were excerpted and record that notied the Ohaeng-acupuncture. Results : The results obtained as follows. 1. ${\ll}$Ling Shu${\gg}$ "Sheng Ze Xie Zhi, Xu Ze Bu Zhi"(盛則瀉之, 虛則補之) united with "Ying Sui Bu Xie"(迎隨補瀉), developed the principle of "Qu Xue" in ${\ll}$Classic on Difficulty${\gg}$. 2. ${\ll}$Classic on Difficulty${\gg}$ explained the interdependent relations, interrestraining relations, the relations of subjugation and reverse restriction in illness condition between the five viscera according to the theory of generation, restriction, subjugation and reverse restriction in five elements. ${\ll}$Classic on Difficulty${\gg}$ united five shu points (五兪穴) with five elements. 3. Zi jing Bu xie according to Xiang Sheng theory is develped from ${\ll}$Classic on Difficulty${\gg}$ "Xu Ze Bu Qu Mu, Shi Ze Xie Qi Zi"(虛則補其母 實則瀉其子) to ${\ll}$Classic on Difficulty${\gg}$ , ${\ll}$Zhen Jiu Ju Ying${\gg}$. 4. Ta jing Bu xie according to Xiang Sheng theory is develped from ${\ll}$Tu Zhu Nan Jing${\gg}$ to ${\ll}$Yi Xue Ru Men${\gg}$. 5. The principle of treatment according to Zi-Ta jing Bu xie based Xiang Ke is develped from ${\ll}$The seventy fifth Difficulty Classic on Difficulty${\gg}$ to Sa Am Do In(舍岩道人).

• Tuberculosis and Respiratory Diseases
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• 제50권5호
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• pp.549-556
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• 2001

연구배경 : 세포성장에 관여하는 여러 growth factor중 IGF-I은 IGF-IR와 결합하여 세포증식을 유발하는 mitogen으로 알려져 있다. 대부분의 정상세포 및 암세포는 IGFBPs을 분비하는데 이들은 IGF-I과 결합하여 IGF-I의 세포증식 효과를 증가 혹은 억제시킨다. 마우스 폐암세포주 (3LL)에서 IGF-I이 세포성장에 미치는 효과를 보고, 3LL 세포에서 분비되는 IGFBP을 추출 확인하고, IGFBPs이 세포 성정에 미치는 영향을 관찰하였다. 방 법 : 마우스 폐암세포주 (3LL)를 이용하여, IGF-I을 투여하여 세포성장을 MTT assay로 측정하였고, 3LL에서 분비되는 IGFBP을 추출하여 Western ligand blot 및 western immunoblot으로 확인하였다. 또한 분비된 IGFBP이 세포성장에 미치는 영향을 보기위해, anti-IGFBP antibody을 첨가하여 이의 기능을 억제하여 세포성장에 관한 기능을 관찰하였다. 결 과 : IGF-I은 serum free media에서 3LL 세포성장을 증가시켰다. 3LL 세포는 IGFBP-4를 생성하는 것을 확인하였고, anti-IGFBP-4 antibody를 첨가시 세포성장이 증가된 소견이 관찰되어 IGFBP-4는 세포증식을 억제하는 기능을 가지고 있음을 간접적으로 알 수 있었다. 결 론 : 이상의 실험 결과로 3LL 마우스 폐암세포에서 IGF-I은 세포성장을 증가시키며, 3LL에서 생성된 IGFBP-4는 세포증식을 억제하는 기능을 가지고 있다는 것을 관찰하였다. 향후 IGF-I과 IGFBPs이 암 성장에 미치는 기전과 임상적 적용에 대한 연구가 필요하리라 생각된다.

• Korean Journal of Acupuncture
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• 제26권2호
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• pp.145-163
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• 2009

Background : Stroke occurrences are prevalent and require intensive care for its treatment and rehabilitation. Acupuncture has been widely used in Korea throughout the history and provides an efficient method in the treatment of it. Objectives : To establish a distinctive and efficient acupuncture method for the treatment of stroke based in literature research. Method : We reviewed four Korean medical literature, $ll$治腫指南Guide to Swollen Sore Treatment$gg$, $ll$東醫寶鑑Treasured Mirror of Eastern Medicine$gg$, $ll$鍼灸經驗方Experiential Prescriptions of Acupuncture and Moxibustion$gg$, and $ll$舍岩鍼法Essential Rhymes on Acupuncture and Moxibustion by Master Sa-am$gg$, and analyzed the therapeutic characteristics in the treatment of stroke. Result : 1. In $ll$治腫指南Guide to Swollen Sore Treatment$gg$, various types of treatment can be found. Besides acupuncture, cupping, moxibustion with moxa tube, and bath therapy using Duchesneae Indicae Herba and Sal was applied. Needling in sublingual and vocal region, and also points like GB20, GB31, LI15, BL60, GV20, GV20, TE4, GB39, LU5, ST36, GB30 were frequently inserted. 2. In $ll$東醫寶鑑Treasured Mirror of Eastern Medicine$gg$, moxibustion treatment were in top priority. Points like GV20, LI15, LI11, GB31, ST36, GB39, LI4, GB20 pertinent to Governor, Conception, Gallbladder, Large Intestine, and Stomach meridian were most frequently needled. Selection of adjacent point was widely applied. 3. $ll$鍼灸經驗方Experiential Prescriptions of Acupuncture and Moxibustion$gg$ has some similarity compared to $ll$東醫寶鑑 Treasured Mirror of Eastern Medicine$gg$ in considering the moxa in top priority. But selected points far from the disease site. Main points were LI4, ST36, GB39, PC5, GV20, LI11, LR3, BL40, HT7. 4. $ll$校勘 舍岩道人鍼法Essential Rhymes on Acupuncture and Moxibustion by Master Sa-am$gg$ having the same content orders with $ll$鍼灸經驗方Experiential Prescriptions of Acupuncture and Moxibustion$gg$, it may had some influence from it. But the differences are also apparent. It emphasized in using mother-supplementing child-draining method, experiential prescriptions and visceral pattern identification. Conclusions : We could find various efficient methods through literature research of medical classics. This will not be limited in stroke alone but also will be applied in other diseases. This study will concurrently result in establishing distinctive therapeutic method characteristic of Korea.