Journal of Korean Society of Forest Science (한국산림과학회지)

Korean Society of Forest Science (한국산림과학회)
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Somatic Embryogenesis and Plant regeneration with Embryogenic Tissue Lines in Larix leptolepis

낙엽송 (Larix leptolepis) 배발생조직 라인에 따른 체세포배 유도 및 식물체 재분화

  • Kim, Yong-Wook (Forest Biotechnology Division, Korea Forest Research Institute)
  • 김용욱 (국립산림과학원 산림생명공학과)
  • Received : 2010.06.16
  • Accepted : 2010.09.03
  • Published : 2010.08.30
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Abstract

This study was conducted to evaluate various effects of kinds of culture medium, concentrations of abscisic acid (ABA) or /kinds of osmotica on maturation of somatic embryos (SEs) with four (LL-L, LL-K, LL-P and LL-N) embryogenic tissue lines (ETLs) in Japanese larch (Larix leptolepis). In comparison of two culture medium, the LL-P produced the highest number of the cotyledon-staged SE (134.9/90 mg tissue) in 1/2LM medium. In contrast, no SEs were obtained except the LL-P (32.9) in medium of BLG. Effects of two concentrations of ABA in the medium with four ETL for SEs maturation were also compared. In the test of 60 or 100 ${\mu}M$ ABA, the highest result was obtained in 60 ${\mu}M$ ABA (142.9). However, the influence of ABA had little on SEs production except the LL-N regardless of concentrations of ABA. In comparison of different kinds/concentrations of osmotica, the best response was obtained from the treatment of 0.2 M maltose, the LL-K (540.5). In conclusion, the effects of production of SEs were greatly rely on the ETLs, rather than kinds of medium, concentrations of ABA or osmotica which were used in maturation of SEs.

본 연구는 낙엽송의 4가지 배발생조직(LL-L, LL-K, LL-P 및 LL-N)으로 체세포배 유도에 영향하는 배지종류, 엡시식산(abscisic acid, ABA)의 농도, 삼투압제의 종류 및 농도의 효과를 평가하기 위해 수행되었다. 배지종류 비교에서 1/2LM 배지에 배양한 LL-P 조직라인(134.9개/90 mg조직)에서 가장 높은 자엽단계의 체세포배가 유도되었으나 BLG 배지의 경우 LL-P라인(32.9개)을 제외하고는 체세포배가 전혀 유도되지 않았다. 2가지 ABA농도(60 혹은 100 ${\mu}M$)에 대한 4가지 배발생조직의 체세포배 유도 효과를 비교 한 바 최대 체세포배는 60 ${\mu}M$ ABA농도(142.9개)에서 나타났으나 LL-N 라인을 제외하고는 체세포배 발생에 ABA영향은 그리 크지 않은 것으로 나타났다. 삼투압제 종류 및 농도 비교에서 최고의 체세포배 발생은 LL-K 조직라인을 0.2 M maltose 첨가배지(540.5개)에 배양하였을 때 나타났다. 결론적으로 체세포배 발생 효과는 배지의 종류, 혹은 배지에 첨가된 ABA 및 삼투압제 농도보다는 배발생 조직 라인에 따라 크게 좌우되었다.

Keywords

References

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