• Asian Pacific Journal of Cancer Prevention
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• v.14 no.7
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• pp.4353-4358
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• 2013

Introduction: Breast cancer aggressiveness can be correlated with proliferation status of tumor cells, which can be ascertained with tumor grade and Ki67 indexing. However due to lack of reproducibility, the ASCO do not recommend routine use of Ki67 in determining prognosis in newly diagnosed breast cancers. We therefore aimed to determine associations of the Ki67 index with other prognostic markers like tumor size, grade, lymph node metastasis, ER, PR and HER2neu status. Methods: A total of 194 cases of newly diagnosed breast cancer were included in the study. Immunohistochemical staining for ER, PR, HER2neu and Ki67 was performed by the DAKO envision method. Associations of the Ki67 index with other prognostic factors were evaluated both as continuous and categorical variables. Results: Mean age of the patients was 51.7 years (24-90). Mean Ki67 index was 26.9% (1-90). ER, PR, HER2neu positivity was noted in 90/194 cases (46.4%), 74/194 cases (38.1%) and 110/194 cases (56.70%) respectively. Significant association was found between Ki67 and tumor grade, PR, HER2neu positivity and lymph node status, but no link was apparent with ER positivity and tumor size. There wasan inverse relation between Ki67 index and PR positivity, whereas a direct correlation was seen with HER2neu positivity. However, high Ki67 (>30%) was associated with decreased HER2neu positivity as compared to intermediate Ki67 (16-30%). The same trend was established with lymph node metastasis. Conclusion: Our study indicates that with high grade tumors, clinical utility of ki67 is greater in combination with other prognostic markers because we found that tumors with Ki67 higher than 30% have better prognostic profile compared to tumors with intermediate Ki67 level, as reflected by slightly lower frequency of lymph node metastasis and HER2neu expression. Therefore we suggest that Ki67 index should be categorized into high, intermediate and low groups when considering adjuvant chemotherapy and prognostic stratification.

• Horticultural Science & Technology
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• v.30 no.5
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• pp.579-585
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• 2012

This study was carried out to investigate the effect of potassium silicate on the growth and leaf epidermal characteristics of horticultural crops viz., begonia (Begonia semperflorens Link et Otto) 'Super Olympia Red' and 'Super Olympia Rose' and pansy (Viola ${\times}$ wittrockiana Hort.) 'Matrix White Blotch' and 'Matrix Yellow Blotch' in vitro. Seeds after germination were grown on a quarter strength MS medium supplemented with potassium silicate ($K_2SiO_3$) at 0, 100, 200, or $300mg{\cdot}L^{-1}$ and were maintained under a photoperiod of 16 hours at $25^{\circ}C$. Growth parameters such as plant height, root length, chlorophyll content, fresh, and dry weights have been recorded after a growth period of 58 days for begonia and 94 days for pansy. In begonia, fresh weight was significantly greatest in the $200mg{\cdot}L^{-1}$ $K_2SiO_3$ treatment in both 'Super Olympia Red' and 'Super Olympia Rose'. In both pansy cultivars, fresh weight was the greatest in the $200mg{\cdot}L^{-1}$ $K_2SiO_3$ treatment than other treatments. Chlorophyll content was significantly greater in the $100mg{\cdot}L^{-1}$ $K_2SiO_3$ treatment for both the cultivars of begonia. Leaf area significantly increased with the higher concentrations of $K_2SiO_3$ treatment in both cultivars of pansy. Stomatal structures on the leaf epidermis were observed with scanning electron microscopy (SEM). In begonia 'Super Olympia Rose', the structure of stomata were more compact in size in the $300mg{\cdot}L^{-1}$ $K_2SiO_3$ treatment than in the control. Similarly, in pansy 'Matrix White Blotch' the surface of stomata appeared to be smoother in the $300mg{\cdot}L^{-1}$ $K_2SiO_3$ treatment than those wrinkled appearance in the control. The surface of the leaf epidermis appeared to be compact due to Si deposition, and thus results indicated that Si positively affected the growth and biomass production of these species. Our data show that the effect of Si on growth parameters is strongly dependent on cultivar of the plant species tested.

• Journal of Korean Society of Transportation
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• v.22 no.7 s.78
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• pp.7-16
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• 2004

The homogeneous road section is defined as one consisted of similar traffic characteristics focused on demand and supply. The criteria, in the aspect of demand, are the diverging rate and the ratio of green time to cycle time at signalized intersection, and distance between the signalized intersections. The criteria, in that or supply, are the traffic patterns such as traffic volume and its speed. In this study, the effective method to generate valuable data, pointing out the problems of removal method of obscure data, is proposed using data collected from Gonjiam IC to Jangji IC on the national highway No.3. Travel times are collected with licence matching method and traffic volume and speed are collected from detectors. Futhermore, the method of selecting homogeneous road section is proposed considering demand and supply aspect simultaneously. This method using outlier filtering algorithm can be applied to generate the travel time forecasting model and to revise the obscured of missing data transmitting from detectors. The point and link data collected at the same time on the rational highway can be used as a basis predicting the travel time and revising the obscured data in the future.

• Journal of Distribution Research
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• v.16 no.5
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• pp.123-140
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• 2011

This study analyzed large distributors' trade features with local suppliers and their direct purchase features through the survey and the interview targeting some MDs(Merchandisers) in large retailers responsible for buying fresh foods (agricultural, fishery, livestock and proceeded products). As the result of conducting the survey targeting the large purchase MDs, it was found that when they select local suppliers, they importantly consider the factors of stable products supply, the quality management, the aggressive management attitude, the affordable (cheap) price, and the suggestion of differentiated goods in order. Concerning their direct purchases in local places, their preference toward the direct-purchase traders were higher than that toward vendors and whole markets as their existing major traders. Among the traders for direct purchase, they preferred farm corporations (including agricultural corporations) the most, and followed the National Agricultural Cooperative, and the unit agricultural cooperative in order. Regarding to the trades with suppliers, when they set the trade price, the merchandisers (MDs) of large distributors preferred long-term trades over 2 years, and utilized the factors of market rate(50.9%), production cost(31.2%) and sale price in the same industry(18.0%). For the local distribution organizations to aggressively counteract the changes of distribution environment in the consumption places, it needs some strategies to organize the agricultural production, to expand the production size and to specialize the distribution channels. And the supply system's continuality and stability are required, so it needs to establish the counteract system enabling to supply all year round through the link of production organizations. Additionally, as an effort to intensify the market negotiation ability, it should collect various market information from the consumption places.

• Asian-Australasian Journal of Animal Sciences
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• v.30 no.12
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• pp.1684-1688
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• 2017

Objective: From a review of published information on genetic association studies, a meta-analysis was conducted to determine the influence of the genes ${\kappa}-casein$ (CSN3) and ${\beta}-lactoglobulin$ (LGB) on milk yield traits in Holstein, Jersey, Brown Swiss, and Fleckvieh. Methods: The GLIMMIX procedure was used to analyze milk production and percentage of protein and fat in milk. Models included the main effects and all their possible two-way interactions; not estimable effects and non-significant (p>0.05) two-way interactions were dropped from the models. The three traits analyzed used Poisson distribution and a log link function and were determined with the Interactive Data Analysis of SAS software. Least square means and multiple mean comparisons were obtained and performed for significant main effects and their interactions (p<0.0255). Results: Interaction of breed by gene showed that Holstein and Fleckvieh were the breeds on which CSN3 ($6.01%{\pm}0.19%$ and $5.98%{\pm}0.22%$), and LGB ($6.02%{\pm}0.19%$ and $5.70%{\pm}0.22%$) have the greatest influence. Interaction of breed by genotype nested in the analyzed gene indicated that Holstein and Jersey showed greater influence of the CSN3 AA genotype, $6.04%{\pm}0.22%$ and $5.59%{\pm}0.31%$ than the other genotypes, while LGB AA genotype had the largest influence on the traits analyzed, $6.05%{\pm}0.20%$ and $5.60%{\pm}0.19%$, respectively. Furthermore, interaction of type of statistical model by genotype nested in the analyzed gene indicated that CSN3 and LGB genes had similar behavior, maintaining a difference of more than 7% across analyzed genotypes. These results could indicate that both Holstein and Jersey have had lower substitution allele effect in selection programs that include CSN3 and LGB genes than Brown Swiss and Fleckvieh. Conclusion: Breed determined which genotypes had the greatest association with analyzed traits. The mixed model based in Bayesian or Ridge Regression was the best alternative to analyze CSN3 and LGB gene effects on milk yield and protein and fat percentages.

• Herbal Formula Science
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• v.18 no.2
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• pp.227-239
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• 2010

Objective : The purpose of this study was to develop the simulator which can analyze the anti-inflammatory effects of herbs based on e-cell, or the virtual cell. Method : We have ensured the medical herbs and its active compounds by investigating the oriental medicine records and NBCI(Biomedicine database). Also we have developed the web-based search system for confirming database related to anti-inflammation. We have researched the cell signal pathway related with inflammatory response control and established the mathematical model of herb interaction on selected signal pathway in e-cell. Finally we have developed the prototype which can confirm the result of this model visibly. Results : We constructed the database of 62 cases of anti-inflammatory active compounds in 61 cases of medical herbs which have been known anti-inflammation effects in the paper, 16 cases of inflammatory factors, 10 cases of signal pathways related with inflammatory response and 6,834 cases of URL(Uniform Resource Locator) of referenced papers. And we embodied the web-based research system, which can research this database. User can search basic and detailed information of medical plants related with anti-inflammatory by using information system. And user can acquire information on an active compounds, a signal pathway and a link URL of related paper. Among investigated ten pathways, we selected NF-${\kappa}B$, which plays important role in activation of immune system, and we searched the mechanisms of actions of proteins which could be components of this pathway. We reduced total network into IKK-$I{\kappa}B$ - NF-${\kappa}B$, and completed mathematic modeling by using ordinary differential equations and response variables of $I{\kappa}B-NF-{\kappa}B$ signaling model network which is suggested by Baltimore Group. We designed OED(Ordinary Differential Equation) for response of IKK, $I{\kappa}B$, $NF-{\kappa}B$ in e-cell's cytoplasm and nucleus, and measured whether an active compound of medicinal plants which is inputted by an user would have a anti-inflammation effects in obedience to change in concentration over time. The proposed model was verified by using experimental results of the papers which are listed on NCBI.

• Animal cells and systems
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• v.16 no.1
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• pp.11-19
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• 2012

A major concern regarding the collection and storage of biodiversity information is the inefficiency of conventional taxonomic approaches in dealing with a large number of species. This inefficiency has increased the demand for automated, rapid, and reliable molecular identification systems and large-scale biological databases. DNA-based taxonomic approaches are now arguably a necessity in biodiversity studies. In particular, DNA barcoding using short DNA sequences provides an effective molecular tool for species identification. We constructed a large-scale database system that holds a collection of 5531 barcode sequences from 2429 Korean species. The Korea Barcode of Life database (KBOL, http://koreabarcode.org) is a web-based database system that is used for compiling a high volume of DNA barcode data and identifying unknown biological specimens. With the KBOL system, users can not only link DNA barcodes and biological information but can also undertake conservation activities, including environmental management, monitoring, and detecting significant organisms.

• Journal of Korean Medical classics
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• v.25 no.1
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• pp.1-16
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• 2012

"Yixuerumen" is a comprehensive medical text published in the Ming-dynasty by Li Chan(李梴). In this text, Sanghan(傷寒, cold damage) is categorized among external contraction(外感) with much emphasis. The subject of this study is the verse "The Principal and secondary aspects must first be decided." and its annotations in the in the Sanghan chapter of "Yixuerumen". The complex theoretical structure of this verse was firstly analyzed, together with the historical background of how and why Li Chan adopted this concept. The Pyo-Bon concept is the contrast between phenomena(標) and its underlying source of motivation(本). The methodology for this study was to compare and analyze this main verse with contents on Sanghan and Un-gi(運氣) within the text, while reviewing historical theories explaining the physiology and pathology of the human body in terms of the Pyo-bon(標本) concept. As a result, we discovered that the Pyo-bon(標本) concept used in the aforementioned verse of "Yixuerumen" matches the Three Eum Three Yang(三陰三陽)-標本中氣(pyo-bon-jung gi)-gi transformation(氣化) theory of Un-gi(運氣). Li Chan created the connecting link in understanding the Three Eum Three Yang diagnosis system through the viscera/bowels theory(臟腑論) by adopting the Three Eum Three Yang(三陰三陽)-標本中氣(pyo-bon-jung gi)-gi transformation(氣化) theory from Un-gi. Li's work lead to several changes in the field of Sanghan. First, Li understood the disease pattern of Sanghan by using the accumulated knowledge of the viscera/bowel theory during the Jin-Yuan dynasty, and developed a medical perspective that observes the disease pattern based on the body's essence gi(精氣). Second, he set the category of the Sanghan-Three Eum Three Yang disease pattern, establishing a separate guideline. Third, by adding knowledge of herbs to the accumulated knowledge of the viscera/bowel theory, the process of diagnosis and herbal application were made explicable. On the other hand, in the process of interpreting the 三陰三陽 diagnosis system with viscera/bowels theory, theoretical inconsistencies appeared, of which Li tried to mend by several means. The results of the research on "Yixuerumen(醫學入門) the Sanghan chapter(傷寒篇)" calls for further studies, as it has effected both "Dongeuibogam(東醫寶鑑) the Sanghan part(寒門)" and "Dongeuisoosebowon(東醫壽世保元)" as well.

• Korean Journal of Weed Science
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• v.16 no.3
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• pp.194-199
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• 1996

Seed size and days required to reach specific growth stages were determined for Echinochloa crus-galli (L.) P. Beauv. varieties which are major grass species found in Korean paddy field and the results were compared with E. frumentacea (Roxb.) Link. In addition, responses of the Echinochloa species on preemergence herbicides (molinate, esprocarb, and mefenacet) were investigated with respect to time of application. One thousand seed weight of E. crus-galli var. oryzicola was about 3 times heavier than that of var. praticola, but was not significantly different that of E. frumentacea. Days requited to reach the 2.5 leaf-stage (LS) were 11, 13, and 14 days after seeding for E. frumentacea, E. crus-galli var. oryzicola, and E. crus-galli var. praticola, respectively. E. crus-galli var. praticola was more sensitive to molinate, esprocarb and mefenacet than var. oryzicola and the sensitivity of both the varieties decreased as the time of application LS was delayed. However, there was no great difference in the response for E. frumentacea when applied between 1.5 and 3.0 LS. In thiocarbamate hebicides esprocarb was more active to Echinochloa species than molinate and the higher the active ingredient of the herbicides, the greater was the activity to Echinochloa species. Differential susceptibility of Echinochloa species to the herbicides was not related to the seed size, but to the early growth stages at the time of herbicide application which were affected by the seed size.

• Korean Institute of Interior Design Journal
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• v.16 no.6
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• pp.152-160
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• 2007

The purpose of this study is to compare conditions of Korean Restaurants in Hong Kong with those of Thai ones, which have already succeeded in globalization, by investigating and analyzing the characteristics of the two parties in Hong Kong, a world-wide international city. For these purposes, we selected 10 Korean restaurants & 10 Thai restaurants in Hong Kong, and visited the selected restaurants during the period from 2007.7.9 to 2007.7.14, to examine indoor spaces and food-related elements were recorded in detail, photographed and analyzed. The results of study is as follows ; we have first found that Korean restaurants did not show any humane properties linked to restaurant concepts and indoor space, did link Korean images through their trade names, and showed decorative culture based on traditional house and dietary-life cultural properties based on traditional Korean Bansang table setting at large. On the other hand, 4 (22.5%) out of the entire cases in Thai restaurants showed religious characteristics with strong Buddhist colors revealed, and all cases showed traditional Thai food cultures, from which we could find out dietary-life cultural properties. Second, whereas there were one case of Korean restaurants in which tradition was main and modernity was assistant, five cases in which tradition and modernity were paralleled in value, and two cases in which modernity was main and tradition was assistant, Thai restaurants had one case where tradition was main and modernity was associative, four cases in which tradition and modernity were paralleled in value, and four cases where modernity was main and tradition was assistant. This means that the Thai restaurants did not insist only on tradition, but showed efforts to transmit luxurious and refined Thai images to people from the world. Third, whereas Korean restaurants had to do with all kinds of menu, were equipped with brazierson their tables uniformly, and showed any differences because they did not use different tableware in accordance with foods, Thai restaurants divided the dinner into the hors d'oeuvre, the main, and the dessert or systemized menu structure by categories in accordance with cooking styles, and tableware selection in accordance with menus, table setting, food materials, and food styling using flowers were consistently expressed.