• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2009.05a
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• pp.350-353
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• 2009

In the fatigue analysis and the components design, uncertainties are caused by the variances of geometry data and applied loads, and the scatter of material properties. In this paper, fatigue crack growth life of turbine is evaluated by fracture mechanics and the reliability analysis is accessed by the fist order second moment method and Monte Carlo simulation.

• Journal of Microbiology
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• v.41 no.2
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• pp.95-101
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• 2003

The lipase gene (lipA) and its activator gene (lipB) of Pseudomonas sp. SW-3 were cloned and sequenced. The lipB was found to be present immediately downstream of lipA. The deduced amino acid sequences of lipA and lipB showed a high level of homology to those of other lipases belonging to the family I.1 of bacterial lipases. When lipA was expressed in Escherichia coli using T7 promoter, an active lipase was produced in cells carrying both lipA and lipB, but not in cells harboring only lipA. Recombinant lipase (rPSL) overproduced in an insoluble form was solubilized in the presence of 8 M urea, purified in a urea-denatured form and refolded by removing urea in the presence of the Ca$\^$2+/ ion. rPLS had maximum activity at pH 8.0 and 50$^{\circ}C$, was stable at pHs from 7.0 to 9.0 and below 50$^{\circ}C$, and showed the highest activity toward the p-nitrophenyl ester of palmitate (Cl6).

• Molecules and Cells
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• v.25 no.3
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• pp.385-389
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• 2008

Septins are a family of filament-forming GTP-binding proteins involved in a variety of cellular process such as cytokinesis, exocytosis, and membrane dynamics. Here we report the biochemical and immunocytochemical characterization of a recently identified mammalian septin, SEPT12. SEPT12 binds GTP in vitro, and a mutation (Gly56 to Asn) in the GTP-binding motif abolished binding. Immunocytochemical analysis revealed that wild-type SEPT12 formed filamentous structures when transiently expressed in Hela cells whereas $SEPT12^{G56A}$ generated large aggregates. In addition, wild-type SEPT12 failed to form filaments when coexpressed with $SEPT12^{G56A}$. We also observed that GTP-binding by SEPT12 is required for interaction with SEPT11 but not with itself.

• Tuberculosis and Respiratory Diseases
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• v.85 no.1
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• pp.25-36
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• 2022

Background: Only a few studies directly compared the therapeutic efficacy and safety of two pressurized metered-dose inhalers (pMDIs) in asthma. We analyzed the asthma treatment outcomes, safety, and patient preferences using formoterol/beclomethasone (FORM/BDP), a pMDI with extra-fine particles, compared with formoterol/budesonide (FORM/BUD), another pMDI with non-extra-fine particles. Methods: In this randomized, double-blind, double-dummy parallel group study, 40 adult asthmatics were randomized to FORM/BDP group (n=18; active FORM/BDP and placebo FORM/BUD) or FORM/BUD group (n=22; active FORM/BUD and placebo FORM/BDP). During the two visits (baseline and end of 8-week treatment), subjects were asked to answer questionnaires including asthma control test (ACT), asthma control questionnaires (ACQ), and Quality of Life Questionnaire for Adult Korean Asthmatics (QLQAKA). Lung function, compliance with inhaler, and inhaler-handling skills were also assessed. Results: Ten subjects in the FORM/BDP group and 14 in the FORM/BUD group completed follow-up visits. ACT, ACQ, QLQAKA (a primary outcome), and adverse events did not differ between two groups. We found that the increase in forced expiratory volume in 1 second/forced vital capacity and forced expiratory flow at 25% to 75% of the pulmonary volume in the FORM/BDP group was higher than in the FORM/BUD group. Regarding preference, subjects responded that the flume velocity of FORM/BDP was higher, but more adequate than that of FORM/BUD. They also answered that FORM/BDP reached the trachea and bronchus and irritated them significantly more than FORM/BUD. Conclusion: The use of pMDI with extra-fine particles may relieve small airway obstruction more than the one with non-extra-fine particles despite no significant differences in overall treatment outcomes. Some asthmatics have a misconception about the adequacy of high flume velocity of pMDIs.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.11 no.5
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• pp.25-37
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• 2008

A field survey was performed at the Human-made Wetlands along Boknaecheon of Juam Lake from March 2004 to October 2007 for the purpose of investigating changes of flora for 4 years since its creation. A total of 15 species including 10 families, 13 genera, 12 species and 3 varieties were planted at the Human-made Wetlands along Boknaecheon of Juam Lake which was completed in December 2002. The survey conducted in 2004, 15 months after creation of the Human-made Wetlands, revealed a flora composed of 47 families, 102 genera, 112 species and 19 varieties, 131 taxa in total. In 2007, further increase was registered with 46 species added compared to 2004 reaching 177 species in total. A 4-year monitoring of naturalized plants showed that no quick but constant increase was featured yearly in their number of species with 12 in 2004, 17 in 2005, 18 in 2006 and 19 in 2007. As for changes in life form, 13 species of perennial plants and 2 woody species, 15 in total, were planted initially but, in the survey 2004, 1- or 2-year old therophytes numbered 73, perennial plants 54 and woody species 4, indicating a large change of life form accompanied with increase in overall number of species. The number of 1- or 2-year old therophytes and perennial plants featured a steady increase each year from 2005 to 2007. The distribution ratio of 1- or 2-year old therophytes, however, showed a gradually decreasing tendency while perennial plants tended to increase, instead. As evidenced in changes of flora at the Human-made Wetlands, most of species in it have been determined to be fit or not within 1 to 2 years after its creation and, since the stable settlement of these species onward, its life forms have undergone stepwise changes together with the number of species and individuals in parallel with repeated extinction and penetration of plants.

• Journal of Microbiology and Biotechnology
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• v.27 no.2
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• pp.350-356
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• 2017

We previously reported that the CopA3 peptide (LLCIALRKK, ${\small{D}}-form$) originally isolated from the Korean dung beetle has antimicrobial and immunosuppressive effects. However, the high cost of producing the synthetic peptide, especially the ${\small{D}}-form$, has limited the development of CopA3 for therapeutic purposes. Here, we investigated whether the CopA3 deletion derivative, CopA5, which is composed of only five amino acids (LLCIA) and has the ${\small{L}}-form$ structure, could inhibit the lipopolysaccharide (LPS)-induced activation of macrophages. Peritoneal exudate macrophages (PEM) were isolated from mice and exposed to LPS in the presence or absence of CopA5, and biomarkers of macrophage activation were measured. Our results revealed that LPS-induced nitric oxide (NO) production, tumor necrosis factor $(TNF)-{\alpha}$ secretion, and phagocytic activity of PEM were significantly inhibited by CopA5 treatment. Similar to CopA3, the structurally modified CopA5 peptide had no cell toxicity (as assessed by measurement of cell viability loss and apoptosis) in PEM. Moreover, the LPS-induced upregulation of the activating phosphorylation of signal transducer and activator of transcription 1 (STAT1) was markedly inhibited by CopA5 treatment. These results suggest that, similar to CopA3, CopA5 inhibits macrophage activation by inhibiting STAT1 phosphorylation and blocking the release of NO and $TNF-{\alpha}$. CopA5 may therefore prove therapeutically useful in the realm of immune suppression.

• Journal of architectural history
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• v.19 no.6
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• pp.233-250
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• 2010

Jukseoru as an official pavilion of the government, one of the eight sceneries in Gwndong Area, is located on the cliff over Osipcheon River. This paper interprets form and space of the pavilion with restored old life by analyzing pictures of official party in 18th century in Chosun Dynasty. Every part of the space is occupied by persons by the class and duty: the pricipal guest, nobles, subordinates, gisaengs, court musicians, and guards from the high to the low and from the inside to the outside. Applying the analysis to the Jukseoru pavilion, the noblest lord takes sit on the platform in front of folded screen at main bay under checked ceiling, enclosed by low timber beam. The next northern end bay is a place for subordinates' and servants' waiting for preparation for rice wine. Southern end entrance bay is not a result of later addition but deliberately and originally made for lower place outside the railing of wooden floor: for a waiting woman gisaeng, and subordinates. Outside under the eaves on the platform with bedrock, artificial stone and soil is a place for court musicians, subordinates, and guards. The yard in front of the building is a place for preparing meals by cookers. Every detailed ornamental form is different each other by the place for the occupied class. Existing theory tells that the building, 7 bays gable-and-hipped roof, is a result of structural extention of 2 bays at the 5 bays gabled-roof. However, through the interpretation of old pictures and application to the pavilion, the present Juseoru is not a result of later structural addition, but deliberately designed creation suited to life in hierarchical traditional society.

• Journal of Korean Theatre Studies Association
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• no.49
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• pp.49-71
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• 2013

Melodrama emerged as a form of the moral in the early modern age. As an approach 'the moral' not only means that rewarding virtue and punishing vice, but also refer to a principle of spiritual life and a way of life. -Melodrama theatricalizes a new vision of human life and society through a new type of the virtuous protagonist and sentiment/-ality. -This allows melodrama to be a dominant cultural form in this modern age, beyond the borders of the theater, mass-media, and literature. Virtue and sentiment/-ality are the core elements of melodrama, which differentiate it from tragedy and comedy especially in the structure and effect of the drama. Actually virtue and sentiment/-ality have been a main target of criticism. Virtue has been regarded as a trite quality of the stereotypical protagonist, and sentiment/-ality as a banal emotion which paralyzes an audience's recognition of reality. -However, this thesis regards both virtue and sentiment/-ality as vehicles for showing and sharing the morals of the modern age. First, the virtues of the protagonist included the general and universal ones of the bourgeois -at that times, the bourgeois represented themselves as a human being- such as the responsibility and obedience of a father, a mother, a wife, a husband, a daughter and a son. They also included the professional ethics such as courage, honesty, and justice and so on. The fall or salvation of the protagonist is largely determined by his/her private individual virtue. Second, sentiment/ality is a theatrical device that makes the audience internalize the protagonist's virtue. The protagonist expresses his/her universal virtue sentimentally, and the audience also expresses their virtue by sympathizing with the protagonist's virtue sentimentally. However, the melodramatic protagonist as an individual, is not connected with society, but remains isolated. As a result, s/he has no influence on the society, where s/he can only ends her/his play alone with a happy-ending. S/he is happy alone, or at best happy with his/her own family. On the contrary to this, tragic protagonist usually fixes social disorder through his/her fall. In that sense, we can say that melodrama presents only the half of the human life.

• Animal Bioscience
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• v.34 no.6
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• pp.1061-1069
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• 2021

Objective: This study was conducted to evaluate the effects of feed processing and particle size on growth performance, nutrient digestibility, carcass characteristics, and gastric health in growing-finishing pigs. Methods: A total of 360 growing pigs (22.64±0.014 kg initial body weight [BW]) were allocated to 1 of 6 treatments with 6 replicates by BW and sex, and 10 pigs were housed in one pen in a randomized complete block design. The BW and feed intake were recorded to calculate growth performance. For the digestibility trial, a total of 24 barrows with an initial BW of 33.65±0.372 kg were split into 6 treatments with a completely randomized design. Dietary treatments were designed by a 2×3 factorial arrangement of treatments based on two main factors, particle size (600, 750, 900 ㎛) and feed form (mash and pellet) of diet. Experimental diets were formulated to contain the requirements of the NRC (2012). Results: The BW and average daily gain were not changed by dietary treatments, and the feed intake of finishing pigs (wks 6 to 12) was increased when the pigs were fed a mash diet (p<0.05). For the overall period, the feed efficiency of pigs was improved with the pellet diet (p<0.01) and reduced particle size (p<0.05). The pellet diet had effects on increasing crude fat digestibility (p<0.01) relative to a mash diet, but there was no considerable change in dry matter and crude protein digestibilities by dietary treatments. In the evaluation of gastric health, a trend for an increased incidence of keratinization in the esophageal region was observed as particle size decreased (p = 0.07). Conclusion: Feed efficiency could be improved by pellet diet and reduced particle size. Nutrient digestibility, carcass characteristics, and gastric health were not affected by feed form, and particle size ranged from 600 to 900 ㎛.

• Korean Journal of Plant Resources
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• v.19 no.5
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• pp.640-648
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• 2006

The distribution and status of naturalized plants in Jeju Island were investigated in this study. As results, the naturalized plants in Jeju Island were total of 254 taxa including 47 families, 161 genera, 248 species and 6 varieties. By the growth habits, annuals held the most percentage including 107 taxa (42.13%). Seventy nine taxa (31.1%) were appeared to have erect type in growth forms and 203 taxa (79.9%) are non-clonal growth form in disseminule forms by radicoid. The most of dormancy form in life form were therophytes as 150 taxa (59%), and following as 53 taxa (20.9%) for hemicryptophytes, and 26 taxa(10.2%) for geophytes. Among the naturalized plants, 115 taxa (36.7%) were originated from Europe, 69 taxa (22%) from Asia and 59 taxa (18.8%) from North America. Twenty eight percent of them were introduced as feeds or mixed seeds of imported cereals.