• The Korean Journal of Physiology and Pharmacology
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• v.7 no.6
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• pp.349-355
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• 2003

We previously shown that LES contraction depends on $M_3$ receptors linked to PTX insensitive $G_q$ protein and activation of PLC. This results in production of $IP_3$, which mediates calcium release, and contraction through a CaM dependent pathway. In the esophagus ACh activates $M_2$ receptors linked to PTX sensitive $G_{i3}$ protein, resulting in activation of PLD, presumably, production of DAG. We investigated the role of PLC isozymes which can be activated by $G_q$ or $G{\beta}$ protein on ACh-induced contraction in LES and esophagus. Immunoblot analysis showed the presence of 3 types of PLC isozymes, $PLC-{\beta}1$, $PLC-{\beta}3$, and $PLC-{\gamma}1$, but not $PLC-{\beta}2$, $PLC-{\beta}4$, $PLC-{\gamma}2$, $PLC-{\delta}1$, and $PLC-{\delta}2$ from both LES and esophageal muscle. ACh produced contraction in a dose dependent manner in LES and esophageal muscle cells obtained by enzymatic digestion with collagenase. $PLC-{\beta}1$ or $PLC-{\beta}3$ antibody incubation reduced contraction in response to ACh in LES but not in esophageal permeabilized cells, but $PLC-{\gamma}1$ antibody incubation did not have an inhibitory effect. The inhibition by $PLC-{\beta}1$ or $PLC-{\beta}3$ antibody on Ach-induced contraction was antibody concentration dependent. The combination with $PLC-{\beta}_1$ and $PLC-{\beta}_3$ antibody completely abolished the contraction, suggesting that $PLC-{\beta}1$ and $PLC-{\beta}3$ have a synergism to inhibit the contraction in LES. $PLC-{\beta}1$, -${\beta}3$ or -${\gamma}1$ antibody did not reduce the contraction of LES cells in response to DAG ($10^{-6}$ M), suggesting that this isozyme of PLC may not activate PKC. When $G_{q/11}$ antibody was incubated, the inhibitory effect of the incubation of PLC ${\beta}3$, but not of PLC ${\beta}_1$ was additive (Fig. 6). In contrast, when $G_{\beta}$ antibody was incubated, the inhibitory effect of the incubation of PLC ${\beta}_1$, but not of PLC ${\beta}_3$ was additive. This data suggest that $G_{q/11}$/11 or $G{\beta}$ may activate cooperatively different PLC isozyme, $PLC{\beta}_1$ or $PLC{\beta}_3$ respectively.

• Wind and Structures
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• v.24 no.1
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• pp.1-24
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• 2017

Wind environment in urban residential areas is an important index to consider when evaluating the living environment. However, due to the complexity of the flow field in residential areas, it is difficult to specify the correct inflow boundary conditions in the large eddy simulation (LES). In this paper, the weighted amplitude wave superposition (WAWS) is adopted to simulate the fluctuating velocity data, which satisfies the desired target wind field. The fluctuating velocity data are given to the inlet boundary of the LES by developing an UDF script, which is implemented into the FLUENT. Then, two numerical models - the empty numerical wind tunnel model and the numerical wind tunnel model with spires and roughness elements are established based on the wind tunnel experiment to verify the present method. Finally, the turbulence generation approach presented in this paper is used to carry out a numerical simulation on the wind environment in an urban residential area in Lisbon. The computational results are compared with the wind tunnel experimental data, showing that the numerical results in the LES have a good agreement with the experimental results, and the simulated flow field with the inlet fluctuations can generate a reasonable turbulent wind field. It also shows that strong wind velocities and turbulent kinetic energy occur at the passageways, which may affect the comfort of people in the residential neighborhood, and the small wind velocities and vortexes appear at the leeward corners of buildings, which may affect the spreading of the pollutants.

• Journal of Aerospace System Engineering
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• v.13 no.6
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• pp.1-8
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• 2019

The synthetic turbulence generation model for inlet boundary conditions of subsonic Backward Facing Step (BFS) was investigated. The average u-velocity and Reynolds stress at inlet boundary follows experimental data. Synthetic Eddy Method (SEM), random noise, and uniform flow conditions were implemented relative to the synthetic turbulence generation method. A three dimensional Large Eddy Simulation (LES) was applied for turbulent flow simulation. Turbulent and mean flow characteristics such as flow reattachment length, velocity profiles, and Reynolds stress profiles of BFS were compared with respect to the turbulent effects.

• Journal of the Korean Society of Propulsion Engineers
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• v.19 no.1
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• pp.42-49
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• 2015

The pressure oscillation induced by inhibitor in a solid rocket motor has been investigated by 3D large eddy simulation(LES) and proper orthogonal decomposition(POD). The vortex generation and breakdown at inhibitor are periodically observed between the inhibitor and the nozzle by flow-acoustic coupling mechanism. The excitation of pressure oscillation occurs as the flow impinges on the submerged nozzle head which recirculate in the cavity in rear dome of the motor chamber. The vortex generation frequency is closely related with the shedding frequencies of the detached vorticities at the inhibiter, which fairly compared with the experimental data.

• Journal of computational fluids engineering
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• v.17 no.3
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• pp.11-17
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• 2012

In this study, aerodynamic analyses have been conducted for 4-Bladed Vertical-Axis Wind Turbine (VAWT) configuration and the results are compared with experimental data. Reynolds-averaged Navier-Stokes equation with LES turbulence model is solved for unsteady flow problems. In addition, the computation results by standard k-${\omega}$ and SST k-${\omega}$ turbulence models are also presented and compared. An experiment model of 4-Bladed VAWT model has been designed and constructed herein. Experimental tests for aerodynamic performance of the present VAWT model are practically conducted using the vehicle mounted testing system. Comparison results between the experiment and the computational fluid dynamics (CFD) analyses are presented in order to show the accuracy of CFD analyses using the different turbulent models.

• Journal of computational fluids engineering
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• v.14 no.3
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• pp.115-122
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• 2009

Numerical methods are applied to simulate the smoke behavior in a ventilated tunnel using large eddy simulation (LES) which is incorporated in FDS (Fire Dynamics Simulator) with proper combustion and radiation model. In this study, present numerical results are compared with data obtained from experiments on pool fires in a ventilated tunnel. The model tunnel is $182m(L){\times}5.4m(W){\times}2.4m(H)$. Two fire scenarios with different ventilation rates are considered with two different fire strengths. The present results are analyzed with those from LES without combustion and radiation model and from RANS ($\kappa-\epsilon$) model as well. Temperature distributions caused by fire in tunnel are compared with each other. It is found that thermal stratification and smoke back-layer can be predicted by FDS and the temperature predictions by FDS show better results than LES without combustion and radiation model. The FDS solver, however, failed to predict correct flow pattern when the high ventilation rate is considered in tunnel because of the defects in the tunnel-inlet turbulence and the near-wall turbulence.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2007.04a
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• pp.233-237
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• 2007

Recent experimental data shows that an irregular fuel surface pops up during the combustion test. This may contribute to the agitated boundary layer due to blowing effect of fuel vaporization. Blowing effect can be of significance in determining the combustion characteristics of solid fuel within the oxidizer flow. LES was implemented to investigate the flow behavior on the fuel surface and turbulence evolution due to blowing effect. Simple channel geometry was used for the investigation instead of circular grain configuration without chemical reactions. This may elucidate the main mechanism responsible for the formation of irregular isolated spots during the combustion in terms of turbulence generation. The interaction of turbulent flow with blowing mass flus causes to breakup turbulent coherent structures and to form the small scale isolated eddies near the fuel surface. This mechanism attributes to the formation of irregular isolated sopt on the fuel surface.

• Journal of computational fluids engineering
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• v.10 no.3 s.30
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• pp.48-54
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• 2005

Three-dimensional incompressible flow past an open cavity in a channel is investigated using Detached Eddy Simulation(DES). The length to depth ratio of the cavity is 2 and the Reynolds number defined with the cavity depth is 3,360. The DES methods are based on the Menter's SST model. In the present work, two types of inflow conditions are used: one is RANS profile, the other is LES inflow from another Large Eddy Simulation(LES) of fully developed channel flow. The results are compared with experimental data and LES results in terms of the mean statistics, temporal physics and scalar transport phenomenon of the flow.

• Journal of the Korean Society of Combustion
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• v.6 no.2
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• pp.29-35
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• 2001

Unsteady numerical study has been conducted on combustion dynamics of a lean-premixed swirl-stabilized gas turbine swirl injector. A three-dimensional computation method utilizing the message passing interface (MPI) parallel architecture, large eddy simulation(LES), and proper orthogonal decomposition (POD) technique was applied. The unsteady turbulent flame dynamics are simulated so that the turbulent flame structure can be characterized in detail. It was observed that some fuel lumps escape from the primary combustion zone, and move downstream and consequently produce hot spots. Those flame dynamics coincides with experimental data. In addition, basis modes of the unsteady turbulent flame are characterized using proper orthogonal decomposition (POD) analysis. The flame structure based on odd basis modes is apparently larger than that of even ones. The flame structure can be extracted from the summation of the basis modes and eigenvectors at any moment.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2008.05a
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• pp.67-70
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• 2008

Production and dissipation of turbulent structure in a swirl stabilized combustor was investigated using three-dimensional Large Eddy Simulation analysis. The combustor of concern is the LM6000, lean premixed dry low-NOx annular combustor, developed by GEAE. Inlet condition was based on experimental data. Strong vortex breakdown in main stream, vortex ring proceeding downstream, and the turbulent structure periodically oscillating have been observed. Reasonable agreement was obtained by comparison of the results with experiments and previous LES studies.