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Isolation and Characterization of a Theta Glutathione S-transferase Gene from Panax ginseng Meyer

  • Kim, Yu-Jin;Lee, Ok-Ran;Lee, Sung-Young;Kim, Kyung-Tack;Yang, Deok-Chun
    • Journal of Ginseng Research
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    • v.36 no.4
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    • pp.449-460
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    • 2012
  • Plants have versatile detoxification systems to encounter the phytotoxicity of the wide range of natural and synthetic compounds present in the environment. Glutathione S-transferase (GST) is an enzyme that detoxifies natural and exogenous toxic compounds by conjugation with glutathione (GSH). Recently, several roles of GST giving stress tolerance in plants have demonstrated, but little is known about the role of ginseng GSTs. Therefore, this work aimed to provide further information on the GST gene present in Panax ginseng genome as well as its expression and function. A GST cDNA (PgGST) was isolated from P. ginseng cDNA library, and it showed the amino acid sequence similarity with theta type of GSTs. PgGST in ginseng plant was induced by exposure to metals, plant hormone, heavy metals, and high light irradiance. To improve the resistance against environmental stresses, full-length cDNA of PgGST was introduced into Nicotiana tabacum. Overexpression of PgGST led to twofold increase in GST-specific activity compared to the non-transgenic plants, and the GST overexpressed plant showed resistance against herbicide phosphinothricin. The results suggested that the PgGST isolated from ginseng might have a role in the protection mechanism against toxic materials such as heavy metals and herbicides.

A Change of Awareness on the Ondol System and Architectural Seeking for Increasing Heating Efficiency since the 18th Century Joseon Society (18세기 이후 조선사회의 온돌에 대한 인식변화와 난방효율 증대를 위한 건축적 모색)

  • Chung, Jung-Nam
    • Journal of architectural history
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    • v.27 no.3
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    • pp.15-26
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    • 2018
  • As a result of reviewing various documents and existing researches, since the late Goryeo period, the most active period in the Ondol(溫突, Korean floor heating system) facilities is the 17th century. The phenomenological reason was recovering the buildings destroyed by the Japanese Invasion of Korea in 1592(壬辰倭亂) & the Manchu War of 1636(丙子胡亂), but the underlying cause was an abnormal climate in which a pair of summer and winter cold continued. In the 17th century, as the Ondol facilities grew rapidly without distinction between regions and classes, the supply and demand of fuel caused economic and natural environmental problems. And a negative and positive view on Ondol was suggested. Since the middle of the 18th century, when the demand and supply of Ondol reached its peak, which could no longer increase, a new awareness of Ondol began to grow. The room was called the Panbang(板房) and the Ondol, depending on the material that made up the floor. It was considered natural to have the Ondol from this time on. The Incan(因間) and Jo(竈) that were made to burn were started to be recorded as a kitchen, regardless of size and function. Changes in social awareness of Ondol have led to concerns about heating efficiency. A variety of architectural explorations were conducted. Such a search was later realized in concrete architectural form. There is a double Ondoll structure, and the column spacing is reduced compared to the previous one. The heat buffer space is formed around the Ondol room, and the double window can control the light and the air going in and out.

Economic Feasibility Analysis of Applepear(Ping Guo Li) Farm Development in Yanbian Area in China ('사과배' 원 조성사업의 경제적 타당성분석 - 중국 연변지역을 중심으로 -)

  • Zhao, Lan-Hua;Lim, Jae-Hwan
    • Korean Journal of Agricultural Science
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    • v.29 no.1
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    • pp.55-65
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    • 2002
  • The most representative fruit in Yanbian area is Applepear(Ping Guo Li). It has been a important resource of farm income. According to the economic growth and income Increase of individual consumers, food consumption pattern will be changed from grain to high value cash crops such as Applepear and vegetables. The globalization and adapt ion of free market oriented economic policy of China government have led to change the collective farming system to individual farm management system. The institutional transformation have brought about high productivity of farm products and incomes of farmers. Therefore the plantation area of Applepear and requirement of investment cost for establishment of Applepear farms have increased rapidly since the 1980's, the time of perestroika and glasnost in Chinese economy. In prosperity of Chinese agriculture, individual farmer's decision making as free selection of farmland use, selection of high pay-off crops, free sale of the products in free market and credit support for the project might be the most important factors. In case of Applepear farm development, net present value of the project net benefit was estimated at 55.518won per hectare and financial rate of return was 21%. The benefit/cost ratio of the project was 2.11. Considering 10% of discounting rate or the opportunity cost of capital in China the Applepear farm development project showed us economically feasible in the light of the above efficiency indices. The Chinese government has to support Applepear farm development project financially and institutionally considering the high-payoff benefit of the fruit and farmer's in come increase in the future.

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The Effect of Social Exclusion of Marginal Workers on Mental Health (비정규직 근로자의 사회적 배제가 정신건강에 미치는 영향 - 건설직 일용근로자의 우울을 중심으로 -)

  • Rho, Byeong-Il;Son, Jung-Hwan
    • Korean Journal of Social Welfare
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    • v.63 no.1
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    • pp.113-135
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    • 2011
  • As intense economic competition has led to more flexibility in the labor market, marginal or atypical employment has rapidly grown. Although marginal work represents an important proportion of whole employment, it is still characterized by insecure and unfavorable working conditions. Therefore employees in marginal jobs are highly likely to be subject to social exclusion. In turn, social exclusion puts marginal workers in a high risk of mental health problems. One of major mental health symptoms has been depression. So far, however, little attention has been paid to the effect of social exclusion of marginal employees on mental health problems including depression. Hence this study examines empirically the effect of social exclusion of casual workers in the construction industry, a protype of marginal employees, on depression. Its results indicate that social exclusion has influence upon depression of marginal workers. This study is the first one to delve into the relationships between temporary employment, social exclusion, and depression of marginal employees. Yet much more empirical researches are needed in order to shed brighter light on these relationships.

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A Study on the Changes of Social Meaning of Androgynous Image in Fashion Magazine(I) -Focused Fashion Item and Adornment- (패션잡지에 나타난 양성이미지의 사회적 의미변화에 관한 연구(I) -의복품목과 장식을 중심으로-)

  • 유홍식;황선진
    • The Research Journal of the Costume Culture
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    • v.11 no.6
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    • pp.852-866
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    • 2003
  • The purpose of this study is to analyze how the women's fashion with the androgynous image(fashion item, adornment) which has appeared on our specialized fashion magazine from 1986 through 2000. As a result of this study I drew the following conclusion. First, masculine image led the market in the first stage of mid 1980 in a men's wear oriented trend that the woman wear masculine style jacket, pants and blouse. In hair style, the short hair of the masculine image was used habitually but the make-up was tend to be made heavily. The ambivalence of the sex was also showed up by expressing the feminine image on the masculine image using the accessories like necklace, earing and bracelet. Second, unlike the first stage, it showed that more and more feminine image was accepted in the second stage of early 1990. The jacket, blouse and pants were rooted as the basic item in women's fashion. The feminine style of long hair and natural make-up with the androgynous image were increased. Third, in the third stage of the late 1990, the fashion items to the comfortable dress of light weight as the life style pursuits the activity and convenience. The feminine image were appeared naturally with a long hair style and natural make-up with the androgynous image. The remarkable features in the fashion accessories were that boots and low shoes were preferred by the increase of the masculine image shoes and the sneakers were seen a lot by the effect of the sports boom.

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North Korea's nuclear and missile development and our countermeasures (북한의 핵 및 미사일 개발과 우리의 대응방안)

  • Lee, Hyun Hee;Kim, Gyu Nam
    • Convergence Security Journal
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    • v.17 no.2
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    • pp.127-135
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    • 2017
  • Today, Kim Jong-un, the third-hereditary regime in North Korea, is committing Nuclear Provocation more aggressively than the past when Kim Il-sung and Kim Jong-il dominated. Past South Korea government had suggested plans to restrain the provocation from North Korea and bring stabilization in the Korean Peninsula. However, consequentially it was limited to the primary role of the President. When President Trump takes over the government in February 2017, it has attracted the expectation about the issues occurred on the Korean Peninsula due to the pledge that he promised during the presidential election and his govern style. However, various speeches about the Korean Peninsula that he spoke recently made situations depressed about what South Korean currently encounters. Furthermore, previous regime in North Korea has laid the foundation for Kim Jong-un to be obsessed more on the nuclear and missile which has led him to provoke more imprudently by highlighting the light weight, advanced, and various kinds of nuclear and missiles. Thus, we would like to propose counter measures in order for South Korean government to handle and solve the issues that they encounters by themselves based on North Korea's Nuclear Provocation instead of relying on other countries to get involved and help.

The Effect of Laser Therapy for Diabetic Ulcer : Systematic Review (당뇨병성 궤양의 레이저치료에 대한 효과 : 체계적 문헌고찰)

  • Kang, Ki-Wan;Kang, Ja-Yeon;Jeong, Min-Jeong;Kim, Hong-Jun;Seo, Hyung-Sik;Jang, In-Soo
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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    • v.30 no.4
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    • pp.62-74
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    • 2017
  • Objectives : The purpose of this study is to investigate the effect of laser therapy for diabetic ulcer by using methods of systematic review. Methods : In this review, PubMed, Cochrane library, Web of Science, CNKI, CiNii, J-STAGE, NDSL and OASIS were used as the search engines. The search period is from the start date of the search engine to October 3, 2016. Randomized controlled trials(RCTs) using laser therapy for diabetic ulcer were searched and extracted by two independent researchers. Risk of bias(RoB) of Cochrane was used to assess methodological quality of studies. Results : Finally, five RCTs were selected. The follow-up period ranged from 15 days to 20 weeks. InGaAlP laser, GaAlAs laser and light emitting diode(LED) were used to treat diabetic ulcer. The clinical trials used sham laser irradiation or standard treatment as control in comparison to laser therapy. The endpoints included ulcer size, rate of healing and time to healing with follow-up period. The RCTs demonstrated therapeutic outcomes with no adverse effect. Most items of RoB were unclear and methodological quality was low. Conclusions : Our analysis suggests that laser therapy has therapeutic effects for diabetic ulcer. However, more systematic and stringent clinical trials will be required.

Effects of Preparation Conditions in the Spray Pyrolysis on the Characteristics of Ca8Mg(SiO4)4Cl2:Eu2+ Phosphor (분무열분해 공정의 제조 조건이 Ca8Mg(SiO4)4Cl2:Eu2+ 형광체 특성에 미치는 영향)

  • Han, Jin-Man;Koo, Hye-Young;Lee, Sang-Ho;Kang, Yun-Chan
    • Korean Journal of Materials Research
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    • v.18 no.2
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    • pp.92-97
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    • 2008
  • In spray pyrolysis, the effects of the preparation temperature, flow rate of the carrier gas and concentration of the spray solution on characteristics such as the morphology, size, and emission intensity of $Ca_8Mg(SiO_4)_4Cl_2:Eu^{2+}$ phosphor powders under long-wavelength ultraviolet light were investigated. The phosphor powders obtained post-treatment had a range of micron sizes with regular morphologies. However, the composition, crystal structure and photoluminescence intensity of the phosphor powders were affected by the preparation conditions of the precursor powders. The $Ca_8Mg(SiO_4)_4Cl_2:Eu^{2+}$ phosphor powders prepared at temperatures that were lower and higher than $700^{\circ}C$ had low photoluminescence intensities due to deficiencies related to the of Cl component. The phosphor powders with the deficient Cl component had impurity peaks of $Ca_2SiO_4$. The optimum flow rates of the carrier gas in the preparation of the $Ca_8Mg(SiO_4)_4Cl_2:Eu^{2+}$ phosphor powders with high photoluminescence intensities and regular morphologies were between 40 and 60 l/minute. Phosphor powders prepared from a spray solution above 0.5 M had regular morphologies and high photoluminescence intensities.

Seismic behavior of full-scale square concrete filled steel tubular columns under high and varied axial compressions

  • Phan, Hao D.;Lin, Ker-Chun
    • Earthquakes and Structures
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    • v.18 no.6
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    • pp.677-689
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    • 2020
  • A building structural system of moment resisting frame (MRF) with concrete filled steel tubular (CFST) columns and wide flange H beams, is one of the most conveniently constructed structural systems. However, there were few studies on evaluating seismic performance of full-scale CFST columns under high axial compression. In addition, some existing famous design codes propose various limits of width-to-thickness ratio (B/t) for steel tubes of the ductile CFST composite members. This study was intended to investigate the seismic behavior of CFST columns under high axial load compression. Four full-scale square CFST column specimens with a B/t of 42 were carried out that were subjected to horizontal cyclic-reversal loads combined with constantly light, medium and high axial loads and with a linearly varied axial load, respectively. Test results revealed that shear strength and deformation capacity of the columns significantly decreased when the axial compression exceeded 0.35 times the nominal compression strength of a CFST column, P0. It was obvious that the higher the axial compression, the lower both the shear strength and deformation capacities were, and the earlier and faster the shear strength degradation occurred. It was found as well that higher axial compressions resulted in larger initial lateral stiffness and faster degradation of post-yield lateral stiffness. Meanwhile, the lower axial compressions led to better energy dissipation capacities with larger cumulative energy. Moreover, the study implied that under axial compressions greater than 0.35P0, the CFST column specimens with B/t limits recommended by AISC 360 (2016), ACI 318 (2014), AIJ (2008) and EC4 (2004) codes do not provide ultimate interstory drift ratio of more than 3% radian, and only the limit in ACI 318 (2014) code satisfies this requirement when axial compression does not exceed 0.35P0.

Photodynamic Therapy Using Topically Applied 5-ALA, MAL and CLC for Canine Otitis Externa

  • Lee, Min-Ho;Song, Hee-Sung;Son, Wongeun;Yun, Young-Min
    • Journal of Veterinary Clinics
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    • v.37 no.1
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    • pp.1-8
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    • 2020
  • Canine otitis externa is a common disorder in small animal practice with prevalence up to 20%. In a large percentage of cases, canine otitis externa is a chronic and recurrent disease also associated with drug-resistant bacteria that is difficult to treat with traditional antibiotics. Photodynamic therapy (PDT) is a new strategy to exterminate pathogenic microorganisms such as bacteria and fungi. The purpose of this study was to investigate the effectiveness of photodynamic therapy against canine otitis externa using three photosensitizer (PS); 5-Aminolevulinic acid (5-ALA) and Methyl aminolevulinic acid (MAL) with semiconductor laser diode (SLD, 635nm of wave length), Chlorophyll-lipoid complex (CLC) with light-emitting diode (LED, 660nm of wave length). After PDT, dogs showed improved Otitis Index Score (OTIS) in swelling, exudate, odor, and pain. A result of the cytology test revealed decrease of bacteria and malassezia count in the oil immersion field and colony forming units count. PDT was effective as a bacteriocide of methicillin-resistant Staphylococcus pseudintermedius (MRSP) and a fungicide of Malassezia pachydermatis. MAL and 5-ALA were more effective PS against canine otitis externa than CLC. These results suggest that PDT is a new strategy to exterminate pathogenic microorganisms such as bacteria and fungi. PDT can be considered as a new therapeutic approach for canine recurrent otitis externa and a countermeasure to drug resistance that is a disadvantage of traditional antibiotic and antifungal therapy.