• Laboraroty Animal Research
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• 제34권4호
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• pp.264-269
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• 2018

Cell cycle dysfunction can cause severe diseases, including neurodegenerative disease and cancer. Mutations in cyclin-dependent kinase inhibitors controlling the G1 phase of the cell cycle are prevalent in various cancers. Mice lacking the tumor suppressors $p16^{Ink4a}$ (Cdkn2a, cyclin-dependent kinase inhibitor 2a), $p19^{Arf}$ (an alternative reading frame product of Cdkn2a,), and $p27^{Kip1}$ (Cdkn1b, cyclin-dependent kinase inhibitor 1b) result in malignant progression of epithelial cancers, sarcomas, and melanomas, respectively. Here, we generated knockout mouse models for each of these three cyclin-dependent kinase inhibitors using engineered nucleases. The $p16^{Ink4a}$ and $p19^{Arf}$ knockout mice were generated via transcription activator-like effector nucleases (TALENs), and $p27^{Kip1}$ knockout mice via clustered regularly interspaced short palindromic repeats/CRISPR-associated nuclease 9 (CRISPR/Cas9). These gene editing technologies were targeted to the first exon of each gene, to induce frameshifts producing premature termination codons. Unlike preexisting embryonic stem cell-based knockout mice, our mouse models are free from selectable markers or other external gene insertions, permitting more precise study of cell cycle-related diseases without confounding influences of foreign DNA.

• 한국산림과학회지
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• 제103권1호
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• pp.65-71
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• 2014

병풍쌈(Cacalia firma)은 병풍취라고도 하며 초롱꽃목 국화과에 속하는 다년생 식물로 한국, 중국, 일본에 분포되어있다. 웰빙 삶을 위한 생활식습관의 변화에 따라 기능성 식품과 유기농 채소에 대한 소비자의 요구가 증가하고 있다. 본 연구는 병풍쌈 임간재배를 위한 적정 광조건을 구명하기 위해 1년생 병풍쌈에 대해 차광망을 이용하여 4가지 광조건 25%, 55%, 75%와 95% 차광처리(전광의 50%, 30%, 20%, 10% 상대광도)와 대조구(전광)에서 생육 후 초기 생장과 생리적 반응을 조사 분석하였다. 75% 차광처리구는 대조구와 비슷한 초장, 뿌리생육과 함수율을 보였으나, 잎의 길이, 폭과 면적이 증가하여 엽 건중량과 총 건중량이 증가하였다. 특히 95% 차광처리구에서는 대조구에 비해 근원경과 뿌리길이는 감소하였으며 총 건중량도 감소하였으나, 초장이 약 40% 증가 하였으며 엽 생육과 함수량 또한 증가하였다. 엽면적비(specific leaf area; SLA)와 엽면적률(leaf area ratio; LAR)은 통계적 유의성을 보이며 대조구에 비해 매우 높게 나타나 잎의 두께가 얇고 수광면적이 큰 것으로 나타났다. 이와 같이 병풍쌈은 낮은 광조건에서도 원활한 탄소동화작용을 하고 있는 것으로 나타났다. 본 연구 결과 병풍쌈의 임간재배를 통한 적정 광요구는 차광처리 75~95%(상대광도 10~20%)로 조사되었으며, 산채류로써의 상품성을 고려할 때 임간지역에서 75% 이상 차광재배 함으로써 식감과 생산량을 높일 수 있을 것으로 기대된다. 이 연구결과는 병풍쌈의 임간재배 가능성을 위한 기초자료로 활용할 수 있을 것으로 판단된다.

• Fibers and Polymers
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• 제6권1호
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• pp.35-41
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• 2005

Research and development of nano fiber products is very active over the world. Physical characteristics and dyeing properties of nylon 66 nano fiber were investigated in this study. X-ray diffraction, DSC, analysis of amino end group, and water absorption were performed to get information concerning physical properties of nano fiber. Nylon 66 nano fiber was dyed with high molecular mass acid dyes. Effects of dyeing temperature, pH of dyeing solution, and concentration of acid dyes on dyeing properties such as rate of dyeing and the extent of exhaustion, were examined and compared to those of regu­lar fiber. It was found that nano fiber adsorbed acid dyes at lower temperature, got rapidly dyed, and its extents of exhaustion at specific dyeing temperature were higher than regular fiber. It was also observed that nano fiber could adsorb a large amount of acid dye without a significant loss in the extent of exhaustion. Washing fastness of the dyed nano fiber was lower by $1/2\~1$ grade, light fastness by 1 grade than the dyed regular fiber.

• 한국작물학회지
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• 제46권3호
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• pp.195-201
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• 2001

본 연구는 광안벼를 공시하여 지연일장의 인공기상실(21$^{\circ}C$ 및 24$^{\circ}C$)과 포장 조건에서 파종기(최초 파종 일 4월25일, 최종 파종일 6월 5일) 이동에 따른 온도와 일장 변화가 출엽 및 출수기 변화에 미치는 영향을 검토하여, 일장에 따른 최종엽수 추정모델을 설정하였으며, 이 모델과 온도에 따른 출엽속도 모델을 결합하여 출수기 예측모델을 설정하여 모델의 출수기 추정 정확도를 검증하였다. 1. 포장에서는 4월 25일 이후 파종기가 늦을수록 생육기간 중 평균기온은 높아진 반면, 포장과 인공기상실 양 조건에서 일장감응기간(6엽기부터 유수분화기)중의 평균일장은 짧아졌다. 이에 따라 일장만 관여한 인공기상실에 비해 온도와 일장이 함께 관여한 포장에서 파종시기에 따른 출수소요일수 단축정도가 크게 나타났다. 2. 일장감응기간의 일장이 길어짐에 따라 최종엽수(FNL)가 증가하였으며, 이 관계는 다음과 같은 Rational 함수로 잘 표현이 되었고($R^2$=0.98),이를 광안벼의 최종엽수 추정모델로 설정하였다. 여기서 D는 일장이며, a,b,c는 상수로서 각각 14.694, -0.992, -0.068로 추정되었다. 3.온도에 따른 출엽속도 모델과 일장에 따른 최종엽수 모델로 구성된 출수기 예측 모델을 구성하였으며, 모델 설정에 이용되지 않은 포장실험 자료를 이용하여 검증한 결과 추정된 출수기는 예측오차가 1-2일로 추정 정확도가 높았다.

• 한국연초학회지
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• 제5권2호
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• pp.17-24
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• 1983

Varietal difference of crop stand, growth of each organs and the factors of dry matter Production at different growth stage of three local tobacco varieties (Hyangcho, Youngwolyub and Yonginyub) were investigated in 1979 and 1980. Varietal variation of total standing crops was clear from 45th day after transplanting to end maximum value of photosyntheticinon-photosynthetic organs was Hyangcho: 1.9, Youngwolyub; 1.6, Yonginyub; 2.3. Maximum value of L/S and T/R ratio was clear from 35th day after transplanting to 45th day. RGR, NAR and CGR were in decreasing order of Yonginyub) Youngwolyub)Hyangcho through out growth stage, LAR and SLA were in decreasing from early growth stage to last growth stage, LAI was obtained to maximum value in the large growth state and LAI was increasing order of during the total growth period that it was Hyangcho is 7.6. Youngwolyub is 9.1 and Yonginyub is 8.7, SLA was Hyangcho;$2.088\textrm{cm}^2$/g/leaf, Youngwclyub;$2.173\textrm{cm}^2$/g/leaf and Yonginyub;$2.020\textrm{cm}^2$/g/leaf, respectively.

• 천문학논총
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• 제12권1호
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• pp.35-45
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• 1997

SOFT($\underline{So}lar\;\underline{F}lare\;\underline{T}elescope$) installed at BOAO(Bohyunsan Optical Astronomy Observatory) is purposed for observing solar active regions using four refractors on single mount with a $400"\times300"$ field of view: Two refractors with a diameter of 15cm(f15) are observe the white light and $H\alpha$, and the other two refractors with a diameter of 20cm(f8) are observe the magnetic field distribution and Doppler shifts at the solar chromosphere. Three Lyot filters, one of the most important observational instruments, are installed on the optical rails for VMG, LMG, and $H\alpha$ that possible to very narrow pass band observation under high precision stability of temperature. From the combination of KD*P and quarter wave plate in the Lyot filter possible observe the magnetic fields strength and doppler shifts by using the characteristics of polarization components. In this paper, we introduce the basic characteristics, optical system, and monitor system of the SOFT.

• 한국대기환경학회지
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• 제29권5호
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• pp.682-691
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• 2013

The aerosol extinction vertical profile and surface visibility have been derived from the Microtops-II sunphotometer observation during the winter 2011 intensive observation period (IOP) at Seoul, Korea. Using models of degradation of aerosol optical thickness (AOT) and aerosol scale height, we have performed extinction-visibility modulation to determine the height dependent aerosol extinction and visibility. It is shown that the aerosol loading is relatively low during IOP (mean $AOT_{550}=0.22{\pm}0.08$, ${\AA}$ngstr$\ddot{o}$m exponent=$1.14{\pm}0.26$). Modeled extinction by use of Microtops II sunphotometer data shows good agreement with measurements by the Multi-wavelenth Polarization Lidar (MPoLAR), and the derived surface visibility are consistent with data from the transmissometer. These results emphasize the use of a vertically resolved extinction from AOT to predict visibility conditions at ground level.

• Fisheries and Aquatic Sciences
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• 제2권2호
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• pp.161-166
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• 1999

The rigor-mortis progress of cultured olive flounder spiked at the brain started much faster than that of wild one. They attained full rigor state after 30 hrs at $0^{\circ}C$, 36 hrs at $5^{\circ}C$ and 50 hrs at $10^{\circ}C$ in the cultured flounder, while after 36 hrs at $0^{\circ}C$, 50 hrs at $5^{\circ}C$, and 60 hrs at $10^{\circ}C$ in the wild. ATP concentration in the muscle was around $5.9\mu mol/g$ for wild and $6.2\mu mol/g$ for cultured flounder. ATP breakdown progressed rapidly in $0^{\circ}C$ samples, followed by $5^{\circ}C$ and $10^{\circ}C$ samples. $Mg^{2+}$-ATPase activity of myofibrillar protein in the presence of 0.25mM CaCb was higher in cultured myofibri1lar protein than in wild one. $Mg^{2+}$-ATPase activities of myofibrillar protein increased during storage in samples stored at $0^{\circ}C$ and $5^{\circ}C$ while decreased in samples stored at $10^{\circ}C$. The level of breaking strength of muscle immediately after death was higher in the wild muscle than in the cultured muscle. The breaking strength reached maximum level at 10 hrs after death in both samples.

• 한국자원식물학회지
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• 제11권1호
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• pp.93-100
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• 1998

This study was to investigate the dry weight, the amount of Na+ and K+ water potential and leaf photosynthesis rate in plants for determining the salt tolerance mechanism in rice cultivars on soil and solution culture with NaCl. The results obtained in this study are summarized as follows ; In general, rice cultivars, cv. Tetep and Jinbu, having high salt tolerance in ID(identified on dry matter production level) showed the higher salt tolerance in RGR (relative growth rate), compared with rice cultivars(cv. Nonglim 41ho, Dunraebyeo and Sobackbyeo) having low salt tolerance. The contents of Na in rice differed depending on culivars and plant parts. Tetep contained 2.9times higher amounts of Na+ than leaf blade and root part. High salt tolerance cultivar Obongbyeo showed a larger decrease in osmotic potential than low salt tolerance cultivar Dunraebyeo suggesting that osmotic adjustment was developed under salt stress conditions in a salt tolerant cultivar . In order to know the IY(identified on grain yeild level using rice cultivars having different salt tolerance the capacity of photosyntheiss was investigated. The capapcity of photosynthesis in cv. Tetep and Obongbyeo having high salt tolerance was much higher that in cv.Dunraebyeo and Nonglim 41 having low salt tolerance.

• Laboraroty Animal Research
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• 제34권4호
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• pp.216-222
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• 2018

The efficacy of standard therapeutic strategies for Helicobacter pylori (H. pylori) infection is decreasing over time due to the emergence of drug-resistant strains. As an alternative, the present study investigated the capacity of Lactobacilllus paracasei (L. paracasei) HP7, isolated from kimchi, to inhibit H. pylori growth. The effects of L. paracasei HP7 on H. pylori adhesion and H. pylori-induced inflammation were examined in AGS human gastric adenocarcinoma epithelial cells and a mouse model of H. pylori SS1 infection. L. paracasei HP7 reduced H. pylori adhesion to AGS cells and suppressed the inflammatory response in infected cells by downregulating interleukin-8. H. pylori colonization in the stomach of C57BL/6 mice was demonstrated by rapid urease test, and results showed significant decrease in mice post-treated with L. paracasei HP7. Additionally, L. paracasei HP7 decreased gastric inflammation and epithelial lesions in the stomach of H. pylori-infected mice. These results demonstrate that L. paracasei HP7 treatment can inhibit H. pylori growth and is thus a promising treatment for patients with gastric symptoms such as gastritis that are caused by H. pylori infection.