• Transactions of the Korean Society of Mechanical Engineers A
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• v.40 no.1
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• pp.17-22
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• 2016

A voice coil actuator with moving coil type for scanning mirror system of barcode reader has been developed. The actuator has a simple structure including a magnet, a coil and a pin. The performance of the actuator is analyzed by a linearized theoretical model. And the dynamic performance of the proposed actuator is predicted through motor constant and restoring constant obtained by finite element simulations. The theoretical model was verified by the prototype which has 64 Hz resonance frequency and 60 deg reflecting angle. We also discovered that that 3 V input can make the actuator rotate over 61.8 deg reflecting angle at 50 Hz resonance frequency. The proposed actuator can simplify its driving configuration because of its implementation of open-loop control.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 2017.04a
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• pp.7-7
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• 2017

본 연구는 수확 작업에 따른 농업용 트랙터의 차축 부하 분석을 목적으로 수행되었다. 트랙터의 부하 측정을 위한 부하 계측 시스템은 4개 차축의 토크 측정을 위한 휠 토크미터, 부하 데이터 수집을 위한 데이터 수집장치, 토크미터에 전원을 공급하기 위한 전원공급장치, 트랙터 배터리를 이용하여 노트북 및 전원공급장치에 전원을 공급하기 위한 인버터, 데이터 처리를 위한 노트북으로 구성하였다. 본 연구에서는 부하계측을 수행하기 위해 동양물산(주)의 60 마력급 트랙터 T623을 사용하였다. 부하 분석은 수확 작업에 따른 주행 단수 별 데이터를 이용하여 통계분석을 수행하였다. 수확 작업 시 L2단에서 좌측 전륜에서의 최대, 최소, 평균, 표준편차 토크는 각각 1735.44, 818.36, 1201.38, 175.53 Nm로 나타났으며, 우측 후륜에서는 3685.11, 1971.68, 2981.80, 258.36 Nm로 나타났다. L3단에서 좌측 전륜에서의 최대, 최소, 평균, 표준편차 토크는 각각 1580.94, 701.06, 1007.93, 125.11 Nm로 나타났으며, 우측 후륜에서는 3239.57, 1735.44, 2472.11, 307.85 Nm로 나타났다. 트랙터의 차축 부하는 L2단에서 L3단으로 작업속도가 증가함에 따라 좌측 전륜에서 약 0.83배, 우측 후륜에서 약 0.82배 감소하는 것으로 나타났다. 향후에는 Romax_Designer (Version 17, Romax Technology, UK)을 사용하여 작업 부하에 따른 트랙터 트랜스미션 해석을 하여 트랙터 PTO 최적설계 가능성을 제시할 계획이다. 정격 출력 조건에서 시뮬레이션을 진행하여 트랙터 트랜스미션을 설계하면 안전성을 확보할 수 있지만 최적설계를 위해서는 실제 포장시험 데이터를 이용한 시뮬레이션이 필요하다고 판단된다.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.11 no.2
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• pp.10-18
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• 2008

In this study, about 80 Kentucky bluegrass (Poa pratensis L.) ecotypes native to Korea were collected annually to analyze their morphology and growth characteristics from 1995 to 2007. Kentucky bluegrass ecotypes with superior characteristics of 'Pureundle' (Trial No. : P38), 'Sewon' (P77), 'Inje' (00034), 'Donggang' (00052), 'Hwasun' (01001), 'Pyengchang' (01071), 'Kongju' (02006), 'Chungsong' (02077), and 'Kumsan' (03005) and three foreign cultivars were transplanted to flowerpots in May 8, 2006 in order to analyze the blooming and morphological characteristics. Out of the selected superior ecotyes, six were collected on the roadsides, two were collected around paddy or dry fields, and one was collected at riverside. Length of 1st internode of 'Chungsong' was the longest (30.7cm), that of 'Pyengchang' was the shortest (12.8cm), and ecotypes showed variation in length. The 1st angle of branches of 'Donggang' was the narrowest ($141.7^{\circ}$), that of 'Hwasun' was the widest ($188.3^{\circ}$), and that of the introduced foreign cultivars were between $159.3^{\circ}$ and $166.7^{\circ}$. The number of nodes of the introduced cultivars were same (six), while that of Korean ecotypes were four to six. Length of flowering culm of 'Chungsong' was the longest (50.7cm) and while that of 'Pyengchang' was the shortest (19.2cm). 'Donggang' with the highest seed yield showed relatively high (36.8cm) inflorescence height when compared to other ecotypes. The five ecotypes of 'Sewon', 'Inje', 'Donggang', 'Pyengchang', and 'Kongju' were investigated to have relatively wide leaf width. Especially 'Sewon' and 'Inje' were investigated to have wide leaves of 4.5mm and over.

• Journal of Navigation and Port Research
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• v.30 no.5 s.111
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• pp.331-334
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• 2006

In this paper, we designed and fabricated the lowpass filter with the wide stopband characteristics using microp- strip line. Adding the $\lambda_g/4$ open stub at the input and output ports, we developed the L-C ladder type lowpass filter using the open stub which has wide stopband characteristics. In order to reduce the entire size qf this filter, the high impedance microstrip lines were made with the meander structure. The lowpass filter was fabricated with the cutoff frequency 2.3 GHz and its measured frequency responses agree well with the simulation results.

• Microbiology and Biotechnology Letters
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• v.50 no.2
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• pp.228-234
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• 2022

Helianthus tuberosus is perennial plant as Compositae family and is shown various physiological activities such as analgesic, antipyretic, anti-inflammatory, anti-fungal, anti-spasmodic, aperient, cholagogue, diuretic, spermatogenic, stomachic, and tonic effects. In this study, we investigated the antidiabetic and anti-inflammatory effects of pancake mixture powder (PM) supplemented with H. tuberosus (PMH) in rat skeletal muscle L6 cells and murine macrophage RAW 264.7 cells, respectively. PM and PMH inhibited in vitro α-glucosidase activity. Glucose consumption was increased by PM and PMH without cytotoxicity in rat myoblast L6 cells. Western blot analysis revealed that PM and PMH down-regulated glycogen synthase kinase (GSK)-3β activation in L6 cells. PM and PMH inhibited inflammatory mediator, nitric oxide (NO) production without cytotoxicity in LPS-induced RAW 264.7 cells. The anti-diabetic and anti-inflammatory effects of PMH was more stronger than those of PM. Anti-diabetic and anti-inflammatory effects of PMH would be due to functional characteristics of the supplemented H. tuberosus and the presence of garlic and onion used as ingredients of PM. Taken together, our results that addition of functional materials such as H. tuberosus in product has synergic effects and PMH is potential candidate for treatment of diabetes through inhibiting inflammation.

• Journal of Embryo Transfer
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• v.15 no.3
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• pp.247-253
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• 2000

This study was undertaken to optimize enucleation and reconstitution methods for the production of cloned mice by somatic cell nuclear transfer Outbred ICR mouse oocytes at the metapahse- II stage were retrieved from female mice superovulated by PMSG and hCG. In Experiment 1, oocytes were enucleated in medium supplemented with cytochalasin B (CCB) of 3 levels (0, 7.5 or 15 $\mu\textrm{g}$/mL), and higher rate of encleation was obtained at 7.5 and 15 $\mu\textrm{g}$/mL than at $\mu\textrm{g}$/mL. In Experiment 2, oocytes enucleated in 7.5 $\mu\textrm{g}$/mL CCB-containing medium were reconstituted with different types of somatic cell by following methods; 1) cumulus cells by direct cell injection, 2) cumulus cells by electric fusion (1.25 kV/cm, 2 pulses for each 70 $mutextrm{s}$) or 3) STO cells by the electrofusion. Electrofusion of STO cells with enucleated oocytes yielded the greatest (P<0.05) rate of reconstitution without lysis (76%) than any other combinations. Although significant decrease in the rate of somatic cell introduction was found, the electrofusion of cumulus cells yielded better rate of reconstitution than direct injection (0 vs. 18%). In Experiment 3, the duration of electric stimulation for the fusion was changed to either 50 $mutextrm{s}$ or 90 $mutextrm{s}$, but no significant improvement of reconstitution efficacy was obtained. In conclusion, this study showed that ICR mouse oocytes could be used for the production of reconstituted oocytes and a fusion method of 1.25 KV/cm with 2 pulses using 570 cell was the optimal.

• Food Engineering Progress
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• v.13 no.1
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• pp.64-69
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• 2009

From the 25 fresh fruits and vegetable products, 1,250 isolates grown on MRS agar media were screened for the inhibitory activity on the growth of Escherichia coli 0157:H7, Listeria monocytogenes, and Bacillus cereus as well as Lactobacillus plantarum, L. casei, and Lactococcus lactis subsp. lactis. Among them, 607 isolates (49% of total isolates) from 23 different foods produced growth inhibitory activity on the E. coli 0157:H7, L. monocytogenes, or B. cereus. When these isolates were screened for the inhibitory activity on the growth of L. plantarum, L. casei, and Lactococcus lactis subsp., 24 isolates (3% of total isolates) from 7 food samples showed bacteriocin-like activity. These isolates had typical physiological characteristics of lactic acid bacteria, which indicated these isolates were strains of lactic acid bacteria. The inhibitor from 3 out of 24 revealed as nicin. From the RAPD-PCR profiles, 24 strains was classified and it was also indicated that most of the strains isolated from same produce showed similar phylogenetic profile.

• Korean Journal of Organic Agriculture
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• v.22 no.3
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• pp.469-481
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• 2014

IIn vitro antimicrobial and anti-inflammatory activities of nargenicin and its derivatives were investigated. Nargenicin, an unusual macrolide antibiotic with potent anti-MRSA (methicilin-resistant Staphylococcus aureus) activity, was purified from the culture broth of Nocardia sp. CS682. And variety of novel nargenicin derivatives was synthesized from nargenicin. Two compounds (4 and 5) exhibit a broad spectrum of antimicrobial activities against infectious bacteria. The antimicrobial activity of derivatives against fifteen organisms was assessed using the minimum inhibitory concentration (MIC). The MIC values were in the ranges of $0.15{\sim}80{\mu}g/mL$ (w/v) for compound 1 and 2, $5{\sim}80{\mu}g/mL$ (w/v) for compound 3, $1.25{\sim}40{\mu}g/mL$ (w/v) for compound 4, and $1.25{\sim}80{\mu}g/mL$ (w/v) for compound 5, depending on the pathogens studied. In vitro, we investigated cytotoxicity and inhibition of nitric oxide (NO) production of synthesized compounds 1-5 in Raw 264.7 cells. LPS-induced nitric oxide releases were significantly blocked by compound 3, 4 and 5 in a dose-dependent manner. At high concentrations ($5{\mu}g/mL$) compound 5 inhibited the NO production by 95%. Compound 4 inhibited the release of NO in LPS-activated Raw 264.7 cells by 75% at the concentration of $10{\mu}g/mL$. Compound 3 inhibited the release of NO in LPS-activated Raw 264.7 cells by 65% at the concentration of $100{\mu}g/mL$. On the other hand, nargenicin, compound 1 and 2 did not inhibit NO production. These results demonstrated that compound 4 and 5 displayed antimicrobial activity and blocked LPS-induced pro-inflammatory mediators such as NO in macrophages, which might be responsible for its therapeutic application.

• IMMUNE NETWORK
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• v.13 no.3
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• pp.81-85
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• 2013

The mucosal surfaces are constantly exposed to incoming pathogens which can cause infections that result in severe morbidity and/or mortality. Studies have reported that mucosal immunity is important for providing protection against these pathogens and that mucosal vaccination is effective in preventing local infections. For many years, the sublingual mucosa has been targeted to deliver immunotherapy to treat allergic hypersensitivities. However, the potential of vaccine delivery via sublingual mucosal has received little attention until recently. Recent studies exploring such potential have documented the safety and effectiveness of sublingual immunization, demonstrating the ability of sublingual immunization to induce both systemic and mucosal immune responses against a variety of antigens, including soluble proteins, inter particulate antigens, and live-attenuated viruses. This review will summarize the recent findings that address the promising potential of sublingual immunization in proving protection against various mucosal pathogens.

• Bulletin of the Korean Chemical Society
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• v.3 no.1
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• pp.13-18
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• 1982

The new substituent constants $({\sigma}^{\ast})$ are calculated from the acidity constants $(pK^{\ast})$ of phenol derivatives in the excited state ($^1L_b$). These substituent constant are applied to the Hammett equations and found good correlation with $pK^{\ast}$ of 2,6-di-tert-butyl phenol, benzamide, nitroaniline, thiophenol, azobenzene, and benzoic acid derivatives. The correlation was much better than that of ground state substituent constants such as ${\sigma},\;{\sigma}^+$, and ${\sigma}^-$. From these results, the new substituent constants $({\sigma}^{\ast})$are proposed to be used for the linear free energy relationship in the $^1({\pi},{\pi}^{\ast})$ excited states of phenyl compounds.