Journal of Embryo Transfer (한국수정란이식학회지)
- 제15권3호
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- Pages.247-253
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- 2000
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- 2508-755X(pISSN)
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- 2288-0178(eISSN)
Improvement of Somatic Cell Nuclear Transfer Technology for the Production of Disease Model Mouse: I. Optimization of Oocyte Enucleation and Reconstruction
질환모델마우스 생산을 위한 체세포핵이식방법 개선; I. 탈핵 및 재조합난자 생산기법 최적화
- Jun, S. H. (College of Medicine, Pochon CHA University and Infertility Medical Center of CHA General Hospital) ;
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Shim, H. S.
(College of Medicine, Pochon CHA University and Infertility Medical Center of CHA General Hospital) ;
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Chung, H. M.
(College of Medicine, Pochon CHA University and Infertility Medical Center of CHA General Hospital) ;
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Lee, B. C.
(Col. Vet. Med., Seoul Nat'l Univ.) ;
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Lee, E. S.
(Dept. Vet. Med., Kangwon Nat'l Univ.) ;
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Ko, J. J.
(College of Medicine, Pochon CHA University and Infertility Medical Center of CHA General Hospital) ;
- Shin, T. (Col. Vet. Med., Texas A &M Univ.) ;
- Park, C. (College of Medicine, Pochon CHA University and Infertility Medical Center of CHA General Hospital) ;
- Hwang, W. S. (Col. Vet. Med., Seoul Nat'l Univ.) ;
- Cha, K. Y. (College of Medicine, Pochon CHA University and Infertility Medical Center of CHA General Hospital) ;
- Lim, J. M. (School Agr. Biotech., Seoul Nat'l Univ.)
- 전수현 (포천중문의과대학교 의학과-차병원 여성의학연구소) ;
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심호섭
(포천중문의과대학교 의학과-차병원 여성의학연구소) ;
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정형민
(포천중문의과대학교 의학과-차병원 여성의학연구소) ;
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이병천
(서울대학교 수의과대학) ;
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이은송
(강원대학교 수의학과) ;
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고정재
(포천중문의과대학교 의학과-차병원 여성의학연구소) ;
- 신태형 (텍사스 A & M 대학교 수의과대학) ;
- 박찬 (포천중문의과대학교 의학과-차병원 여성의학연구소) ;
- 황우석 (서울대학교 수의과대학) ;
- 차광렬 (포천중문의과대학교 의학과-차병원 여성의학연구소) ;
- 임정묵 (서울대학교 농생명공학부)
- 발행 : 2000.12.01
초록
This study was undertaken to optimize enucleation and reconstitution methods for the production of cloned mice by somatic cell nuclear transfer Outbred ICR mouse oocytes at the metapahse- II stage were retrieved from female mice superovulated by PMSG and hCG. In Experiment 1, oocytes were enucleated in medium supplemented with cytochalasin B (CCB) of 3 levels (0, 7.5 or 15