• Title/Summary/Keyword: L35

Search Result 6,526, Processing Time 0.033 seconds

Probiotic Characteristics of Lactobacillus plantarum FH185 Isolated from Human Feces

  • Park, Sun-Young;Lim, Sang-Dong
    • Food Science of Animal Resources
    • /
    • v.35 no.5
    • /
    • pp.615-621
    • /
    • 2015
  • Lactobacillus plantarum FH185 was isolated from the feces of healthy adults. In our previous study, L. plantarum FH185 was demonstrated that it has anti-obesity effect in the in vitro and in vivo test. In order to determine its potential for use as a probiotic, we investigated the physiological characteristics of L. plantarum FH185. The optimum growth temperature of L. plantarum FH185 was 40℃. L. plantarum FH185 showed higher sensitivity to novobiocin in a comparison of fifteen different antibiotics and showed higher resistance to polymyxin B and vancomycin. It also showed higher β-galactosidase and N-acetyl-β-glucosaminidase activities. Moreover, it was comparatively tolerant to bile juice and acid, and inhibited the growths of Salmonella Typhimurium and Staphylococcus aureus with rates of 44.76% and 53.88%, respectively. It also showed high adhesion activity to HT-29 cells compared to L. rhamnosus GG.

Analysis of Nodakenetin from Samultangs Fermented by Lactose Bactera Strains (유산균 발효에 의한 사물탕들부터 노다케네틴의 분리 및 함량분석)

  • Kim, Dong-Seon;Roh, Joo-Hwan;Cho, Chang-Won;Ma, Jin-Yeul
    • The Korea Journal of Herbology
    • /
    • v.27 no.1
    • /
    • pp.35-39
    • /
    • 2012
  • Objectives : The purpose of this study was to investigate the changes in the contents of constituents in Samultang and its fermentations with 10 species of lactic acid bacteria. Methods : Ten strains of lactic acid bacteria, Lactobacillus casei, L. acidophilus, L. casei, L. plantarum, L. amylophilus, L. curvatus, L. delbruekil subsp. lactis, L. casei, B. breve, and B. thermophilum, were used for the fermentation of Samultang. The increased and decreased constituents were identified using HPLC/DAD and various liquid chromatographic techniques, and the structure was elucidated using NMR and MS. These compounds were quantitatively analyzed using an HPLC/DAD system. Results : A remarkably increased component was identified to be nodakenetin and a decreased component was determined to be nodakenin. The fermentation of the ten lactic acid bacteria demonstrated that the decomposable rate of these two compounds in fermented Samultang were different. Samultang fermented by L. plantarum showed the most remarkable changes. Conclusion : Nodakenetin was identified as bioconversion component after fermentation and L. plantarum was discovered the best bacteria to increase the component.

Identification of Antioxidative Constituents from The Branches of Quercus gilva Blume (개가시나무 가지로부터 항산화성분의 규명)

  • Moon, Mi-Youn;Baik, Jong-Seok;Kim, Sang-Suk;Jang, Won-Jung;Kim, Mi-Sun;Lee, Nam-Ho
    • Journal of the Society of Cosmetic Scientists of Korea
    • /
    • v.35 no.3
    • /
    • pp.251-256
    • /
    • 2009
  • Investigation of antioxidative constituents from the ethanol extract of Quercus gilva branches led to the identification of four compounds; catechin (1), epi-catechin (2), tyrosol (3) and tiliroside (4). The antioxidative activity was examined using DPPH radical and superoxide anion radical scavenging method. The isolated compounds 1, 2, 3 and 4 exhibited 94.2 %, 93.4 %, 33.6 % and 11.2 % scavenging activities respectively against DPPH radicals at the concentration of $100{\mu}L$/mL. As well, the compounds $1{\sim}4$ showed respectively 60.2 %, 35.1 %, 20.6 %, 4.5 % inhibition activities against superoxide anion radicals at $200{\mu}L$/mL. Interestingly, the compounds $1{\sim}4$ were isolated for the first time from Quercus gilva Blume.

Transcriptional profiles of rock bream iridovirus (RBIV) using microarray approaches

  • Myung-Hwa, Jung;Jun-Young, Song;Sung-Ju, Jung
    • Journal of fish pathology
    • /
    • v.35 no.2
    • /
    • pp.141-155
    • /
    • 2022
  • Rock bream iridovirus (RBIV) causes high mortality and economic losses in the rock bream (Oplegnathus fasciatus) aquaculture industry in Korea. Viral open reading frames (ORFs) expression profiling at different RBIV infection stages was investigated using microarray approaches. Rock bream were exposed to the virus and held for 7 days at 23 ℃ before the water temperature was reduced to 17 ℃. Herein, 28% mortality was observed from 24 to 35 days post infection (dpi), after which no mortality was observed until 70 dpi (end of the experiment). A total of 27 ORFs were significantly up- or down-regulated after RBIV infection. In RBIV-infected rock bream, four viral genes were expressed after 2 dpi. Most RBIV ORFs (26 genes, 96.2%) were significantly elevated between 7 and 20 dpi. Among them, 12 ORF (44.4%) transcripts reached their peak expression intensity at 15 dpi, and 14 ORFs (51.8%) were at peak expression intensity at 20 dpi. Expression levels began to decrease after 25 dpi, and 92.6% of ORFs (25 genes) were expressed below 1-fold at 70 dpi. From the microarray data, in addition to the viral infection, viral gene expression profiles were categorized into three infection stages, namely, early (2 dpi), middle (7 to 20 dpi), and recovery (25 and 70 dpi). RBIV ORFs 009R, 023R, 032L, 049L, and 056L were remarkably expressed during RBIV infection. Furthermore, six ORFs (001L, 013R, 052L, 053L, 058L, and 061L) were significantly expressed only at 20 dpi. To verify the cDNA microarray data, we performed quantitative real-time PCR, and the results were similar to that of the microarray. Our results provide novel observations on broader RBIV gene expression at different stages of infection and the development of control strategies against RBIV infection.

Inhibition of Listeria monocytogenes by Low Concentrations of Ethanol (저농도의 Ethanol에 의한 Listeria monocytogenes의 증식억제)

  • 박찬성;김미림
    • Korean journal of food and cookery science
    • /
    • v.11 no.4
    • /
    • pp.379-385
    • /
    • 1995
  • The effect of low concentrations of ethanol (3-7%, v/v) in tryptic soy broth (TSB) as an antibacterial agent against Listeria monocytogenes was tested at -20, 5, 35, 45, 50 and 55$^{\circ}C$. Increasing concentrations of ethanol progressively inhibited initial growth of L. monocytogenes at 35$^{\circ}C$. Growth occured at 5% ethanol, but only after a prolonged lag period. The number of viable cells of L. monocytogenes declined during incubation at 7% ethanol. TSB containing 3-7% ethanol was inoculated with 10$\^$5/-10$\^$6/ cells/$m\ell$ or L. monocytogenes and incubated at low temperatures (5$^{\circ}C$, -20$^{\circ}C$). In the presence of 3% of ethanol at 5 or -20$^{\circ}C$, bacterial growth was inhibited more than 90% of control cells. TSB containing 3-7% ethanol was inoculated with 10$\^$6/-10$\^$7/ cells/$m\ell$ of L. monocytogenes and incubated at high temperatures (45$^{\circ}C$, 50$^{\circ}C$, 55$^{\circ}C$). Decrease in viability of the cells incubated at 45 or 50$^{\circ}C$ was slow and the survival of L. monocytogenes was not affected so much in the presence of 3% of ethanol. The viability of L. monocytogenes was decreased with increasing concentration of ethanol and temperature. Decimal reduction times (D-values) based on tryptic soy agar plates at 55$^{\circ}C$ were 20.1, 12.6, 7.4 and 4.2 min in 0, 3, 5 and 7% ethanol, respectively.

  • PDF

Effect of the Salt Concentration in Seafood Wastewater on the High-Rate Anaerobic Digestion (수산물 가공폐수내 염분농도가 고율 혐기성 소화에 미치는 영향)

  • Choi, Yong-Bum;Han, Dong-Joon;Lee, Hae-Seung;Kwon, Jae-Hyouk
    • Journal of Korean Society of Environmental Engineers
    • /
    • v.35 no.10
    • /
    • pp.730-736
    • /
    • 2013
  • This study was conducted to examine the effects of the salt concentration in seafood wastewater on the high-rate anaerobic digestion process. In the general high-rate anaerobic process test, the TCODcr removal efficiency at 6 hr or more HRT was 81.1~0.7%, and the optimal HRT for seafood wastewater process was found to be 6 hr or more. The methane content in the biogas was 70.1~76.8% during the operation, and was hardly affected by the change in the influent load. The results of the anaerobic digestion efficiency according to the salt concentration showed that the removal efficiency of TCODcr was 83.4~89.2% below a $4,000mgCl^-/L$ salt concentration, and mid-70% at a $5,000mgCl^-/L$ salt concentration. Therefore, the salt concentration had to be kept below $4,000mgCl^-/L$ to ensure stable treatment efficiency. Below a $3,000mgCl^-/L$ salt concentration, the methane generation was 0.2999~0.346$m^3CH_4/kgCODrem.$, which was similar to the theoretical methane gas generation in STP condition ($0.35m^3CH_4/gTCODrem.$). The methane content in the biogas was 64.7~73.3% below a $3,000mgCl^-/L$ salt concentration, but decreased with an increase in the salt concentration, to 50.1~56.9% at a $4,000mgCl^-/L$ concentration.

Hematologic and biochemical reference intervals for natural monument Korean goral (Naemorhedus caudatus) (천연기념물 한국 산양의 혈액 및 혈액 화학적 정상표준범위)

  • Ahn, Sangjin;Choi, Youngjin;Kim, Jong-Taek
    • Korean Journal of Veterinary Service
    • /
    • v.40 no.2
    • /
    • pp.155-159
    • /
    • 2017
  • Korean goral (Neamorhedus caudatus) is registered as a natural monument number 217 by South Korea Cultural Heritage Administration. It is also recognized as the endangered species I by Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES). In this study, blood samples of Korean goral were collected to make reference intervals. Blood sampling was conducted on 19 numbers of Korean gorals (ten females, nine males), which were reared in Korean Goral Restoration Center. For total samples, the reference intervals of hematological parameters were: white blood cell $7.69{\sim}10.87K/{\mu}{\Lambda}$; hematocrit 36.73~46.18%; red blood cell $10.72{\sim}12.86K/{\mu}{\Lambda}$; hemoglobin 12.79~15.14 g/dL; mean corpuscular volume 33.15~36.75 fL; mean corpuscular hemoglobin 11.53~12.23 pg; mean corpuscular hemoglobin concentration 32.64-35.91 g/dL and red blood cell distribution width 39.2~43.46%. For total samples of each parameters, the following results were obtained for serum biochemistry: glucose 111.81~153.77 mg/dL; blood urea nitrogen 22.35~28.91 mg/dL; creatine 1.22~1.84 mg/dL; phosphate 4.57~6 mg/dL; calcium 8.7~9.1 mg/dL; total protein 6.53~6.92 g/dL; albumin 3.1~3.48 g/dL; globulin 3.26~3.62 g/dL; alanine aminotransferase 56.7~158.56 U/L; aspartate aminotransferase 230.35~473.06 U/L; alkaline phosphatase 178.06~332.47 U/L; gamma-glutamyl transpeptidase 131.6-~181.24 U/L; total bilirubin 1.47~2.12 mg/dL; cholesterol 46.48~71.52 mg/dL; amylase 16.3~26.03 U/L; sodium 150.43~153.88 mmol/L; potassium 3.98~4.6 mmol/L and chlorine 109.48~113.26 mmol/L. The ranges of values were similar campared to previous studies except in the case of RDW value, which showed higher range than the RDW value of a previous study. The reference intervals from this study will be useful data for treatment and management of gorals.

Evaluation of Weldability on Identical and Dissimilar Welding Parts of Austenite Stainless Steel by GTAW (오스테나이트 스테인리스강에 대한 가스텅스텐 아크용접 시동종 및 이종 용접부의 용접성 평가)

  • Han, Min-Su;Park, Jae-Cheul;Kim, Seong-Jong
    • Journal of Advanced Marine Engineering and Technology
    • /
    • v.35 no.1
    • /
    • pp.68-74
    • /
    • 2011
  • This papers investigated the mechanical characteristics and the weldability of identical as well as dissimilar welding by GTAW for STS 304 and STS 316L. It is applied to double wall gas pipe of duel fuel engine for LNG carrier. Consequently, the weldability of dissimilar and identical welded zone of STS 304 decreased compared to base metal significantly. The result of microstructure observation for welded zone, a degree of acicular ferrite in welding zone for STS 304 presented more than STS 316L. The hardness of welding zone for STS 304 presented higher value than that for STS 316L by this effect.

A Study on the Dynamic Feature of Phoneme for Word Recognition (단어인식을 위한 음소의 동적 특징에 관한 검토)

  • 김주곤
    • Proceedings of the Acoustical Society of Korea Conference
    • /
    • 1997.06a
    • /
    • pp.35-39
    • /
    • 1997
  • 본 연구에서는 음소를 인식의 기본단위로 하는 한국어 단어인식 시스템의 인식정도를 개선하기 이해 각 음소의 시간방향의 정보를 포함하고 있는 동적특징인 회귀계수와 K-L(Karhunen-Loeve)변환으로 얻은 특징파라미터(이하 K-L계수라 함)를 이용하여 음소인식과 단어인식 실험을 수행한 결과 그 유효성을 확인하였다. 이를 위해 먼저 파열음을 대상으로 정적 특징과 파라미터인 멜-켑스트럼(Mel-Cepstrum)과 동적 특징 파라미터인 회귀계수(Regressive Coefficient) 와 K-L 계수(Karhunen-Loeve Coefficient)를 추출하여 음소 인식실험을 수행하였다. 그 결과 멜-켑스트럼을 사용한 경우 39.84%, 회귀계수를 사용한 경우 48.52%, K-L계수를 사용한 경우 52.40%의 인식률을 얻었다. 이를 참고로 각각의 특징 파라미터를 결합하여 인식실험한 결과 멜-켑스트럼과 K-L계수를 사용한 경우 47.17%,멜 -켑스트럼과 회귀계수의 경우 60.11%,K-L계수와 회귀계수의 경우 60.35%, 멜-켑스트럼과 K-L계수 , 회귀계수를 사용한 경우 58.13%를 인식률을 얻어 동적특징인 K-L 계수와 회귀계수를 사용한 경우와 멜-켑스트럼과 회귀계수를 사용한 경우가 높은 인식률을 보였으며 이를 단어로 확장하여 인식실험을 수행한 결과 기존의 특징 파라미터를 이용한 경우보다 높은 인식률을 얻어 동적 파라미터의 유효성을 확인하였다

  • PDF