• Proceedings of the Korea Water Resources Association Conference
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• 2016.05a
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• pp.500-500
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• 2016

전국의 농업용 저수지 중 장기간 높은 유기물 및 영양염의 농도로 인해 부영양화가 우려되는 71개 저수지에 대하여 수질오염 및 퇴적물오염 특성을 분석하였다. 수질오염 특성 분석 결과, 평균농도가 COD 11.7 mg/L, TOC 6.8 mg/L, TN 1.844 mg/L, TP 0.101 mg/L, SS 18.1 mg/L, Chl-a 57.3 mg/$m^3$ 로 분석되었다. 조사 대상 저수지 71개 지구 중 COD 46.3 %, TN 84.4 %, Chl-a 63.7 %가 농업용수 수질기준 초과 저수지로 조사되었다. 또한 연구 대상지구의 주요 오염원으로 생활계오염원 31개 저수지의 COD 11.1 mg/L이며, 축산계오염원 40개 저수지의 COD 12.2 mg/L로 나타났다. 각 항목의 수질 결과에 대한 평균 오염도를 분석한 결과, 축산계 오염원이 생활계 오염원 저수지보다 부유물질(SS)을 제외하고 모든 항목에서 오염도가 높게 나타났다. 그러나 TN, TP는 오염원에 따라 크게 차이를 보이지는 않았으며, 축산계 오염원 저수지의 수질 오염도는 모든 항목에서 농업용수 수질기준을 초과하였다. 장기적인 수질오염 저수지에 대하여 퇴적물의 오염 특성을 조사하였으며, 퇴적물오염으로 인한 수질오염 영향관계를 조사 분석하였다. 퇴적물 오염 특성 분석 결과, 강열감량 10.5 %, 총질소 3,480 mg/kg, 총인 1,299 mg/kg으로 오염 수준이 전체적으로 높게 조사되었다. 생활계오염원 31개 저수지의 강열감량 9.8 %, 총질소 2,711 mg/kg, 총인 1,178 mg/kg이며, 축산계오염원 40개 저수지의 강열감량 11.1 %, 총질소 4,076 mg/kg, 총인 1,393 mg/kg로 나타났다. 수질 중 COD, TOC, TN, TP, SS, Chl-a의 오염도와 퇴적물중 강열감량, 총질소, 총인의 각 항목간 상관관계를 분석한 결과, 퇴적물 내 다른 항목에 비해 SS를 제외하고 총인이 모든 수질 항목과 정(+)의 상관관계를 나타내었다. 특히 퇴적물 중 총인과 수질의 TP가 가장 높은 상관성을 나타내는 것으로 조사되었는데, 이는 퇴적물 내 총인이 수질오염에 가장 많은 영향을 줄 수 있으며, 상호작용이 있음을 의미한다고 하겠다. 또한 수질의 TP 오염도에 따라 부영양화 및 조류의 활성화가 크게 영향을 받는다는 기존의 연구결과에 의하면 퇴적물 내 총인의 오염도가 부영양화와 조류 번식에 영향을 크게 미칠 것이라 판단된다.

• Korean Journal of Ecology and Environment
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• v.37 no.2 s.107
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• pp.180-192
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• 2004

This study was carried out to assess the seasonal variation of water quality and the effect of pollutant loading from watershed in a shallow eutrophic reservoir (Shingu reservoir) from November 2002 to February 2004, Stable thermocline which was greater than $1^{\circ}C$ per meter of the water depth formed in May, and low DO concentration (< 2 mg $O_2\;L^{-1}$) was observed in the hypolimnion from May to September, 2003. The ratio of euphotic depth to mixing depth ($Z_{eu}/Z_{m}$) ranged 0.2 ${\sim}$ 1.1, and the depth of the mixed layer exceeded that of the photic layer during study period, except for May when $Z_{eu}$ and $Z_{m}$ were 4 and 4.3 m, respectively. Most of total nitrogen, ranged 1.1 ${\sim}$ 4.5 ${\mu}g\;N\;L^{-1}$, accounted for inorganic nitrogen (Avg, 58.7%), and sharp increase of $NH_3$-N Hand $NO_3$-N was evident during the spring season. TP concentration in the water column ranged 43.9 ${\sim}$ 126.5 ${\mu}g\;P\;L^{-1}$, and the most of TP in the water column accounted for POP (Avg. 80%). During the study period, DIP concentration in the water column was &;lt 10 ${\mu}g\;P\;L^{-1}$ except for July and August when DIP concentration in the hypolimnion was 22.3 and 56.7 ${\mu}g\;P\;L^{-1}$, respectively. Increase of Chl. a concentration observed in July (99 ${\mu}g\;L^{-1}$) and November 2003 (109 ${\mu}g\;L^{-1}$) when P loading through two inflows was high, and showed close relationship with TP concentration (r = 0.55, P< 0.008, n = 22). Mean Chl. a concentration ranged from 13.5 to 84.5 mg $L^{-1}$ in the water column, and the lowest and highest concentration was observed in February 2004 (13.5 ${\pm}$ 1.0 ${\mu}g\;L^{-1}$) and November 2003 (84.5 ${\pm}$29.0 ${\mu}g\;L^{-1}$), respectively. TP concentration in inflow water increased with discharge (r = 0.69, P< 0.001), 40.5% of annual total P loading introduced in 25 July when there was heavy rainfall. Annual total P loading from watershed was 159.0 kg P $yr^{-1}$, and that of DIP loading was 126.3 kg P $yr^{-1}$ (77.7% of TP loading. The loading of TN (5.0ton yr-1) was 30 times higher than that of TP loading (159.0 kg P yr-1), and the 78% of TN was in the form of non-organic nitrogen, 3.9 ton $yr^{-1}$ in mass. P loading in Shingu reservoir was 1.6 g ${\cdot}$ $m^{-2}$ ${\cdot}$ $yr^{-1}$, which passed the excessive critical loading of Vollenweider-OECD critical loading model. The results of this study indicated that P loading from watershed was the major factor to cause eutrophication and temporal variation of water quality in Shingu reservoir Decrease by 71% in TP loading (159 kg $yr^{-1}$) is necessary for the improvement of mesotrophic level. The management of sediment where tine anaerobic condition was evident in summer, thus, the possibility of P release that can be utilized by existing algae, may also be considered.

• Proceedings of the Korea Water Resources Association Conference
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• 2015.05a
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• pp.545-545
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• 2015

금호강은 포항시 북구 죽장면에서 발원하여 낙동강으로 합류하는 낙동강 제1지류로 유로연장은 114.6km이며, 영천과 경산지역의 생활 및 공업용수, 대구지역의 공업용수로 사용된다. 금호강의 평균 BOD는 1983년 191.2mg/L로 하수도나 산업폐수 처리 전 원수 정도의 수준이었으나, 2014년에는 3.6mg/L로 낮아져 수질개선율이 98.1%를 나타냈다. 이를 위해서 하 폐수 처리장 건설과 아울러 포항철강산업단지의 공업용수 공급을 위한 영천댐에 임하댐과 도수터널 52km를 연결하여 2001년부터 금호강에 하천유지용수로 $3.463m^3/s$를 공급하였다. 금호강 유역에서 오염총량제 목표수질은 금호B 지점이 BOD 3.8mg/L, TP 0.236mg/L이며, 금호C 지점은 BOD 4.0mg/L, TP 0.254mg/L이다. 분석기간인 2013년에서 2014년의 평균 수질은 모두 기준을 만족하고 있으나, 수질악화시에 금호B 지점에서 최대 BOD 8.6mg/L, TP 0.511mg/L, 금호C 지점에서 최대 BOD 8.5mg/L, TP 0.449mg/L을 나타내었다. 이에 영천댐의 탄력적인 유량방류로 수질악화시에 추가 방류를 수행하여 수질을 안정화시키고, 물공급의 안정화가 필요한 상황이다. 본 연구에서는 갈수기 및 이수기에 영천댐에서 하천유지용수보다 많은 유량을 탄력적으로 방류하는 가상조건을 설정하여, 하천유지유량과 목표수질을 만족하는 유량분석을 수행하였다. 이를 위해 QUALKO2 모형을 적용하였으며, 상류단 경계조건을 영천댐으로 설정하고 낙동강에 합류하기까지 약 86km 구간에 대해서 유량과 수질모의를 수행하였다. 모형적용을 위해서는 23개의 reach와 86개 element를 사용하였으며, 주요지류로는 신령천, 청통천, 부기천, 오목천, 남천, 동화천, 신천, 팔거천, 달서천, 이언천를 적용하고, 주요 하 폐수 처리시설을 점오염원으로 입력하였다. 실측자료는 2013년과 2014년의 월자료를 사용하였으며, 유량시나리오는 상류단 영천댐 방류량 조건을 반영하여, 실방류량 조건과 하천유지용수 방류조건, 유지용수 외 추가 25%, 50% 방류조건, 목표수질을 달성하는 방류조건으로 총 5가지의 시나리오를 수행하였다. 상류단 유량이 증가하면 금호강 전반적으로 수질이 개선되나 수질 악화시에는 과도한 방류량을 요구하게 되어, 수질 악화시를 위한 구조적 비구조적 대책도 필요한 상황으로 분석된다.

• Asian-Australasian Journal of Animal Sciences
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• v.26 no.9
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• pp.1339-1346
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• 2013

This study was carried out to determine the effects of tomato powder (TP) on cooked pork patties during storage at $10{\pm}1^{\circ}C$ in the dark. The total phenolic and flavonoid contents of TP extract were 26.22 mg gallic acid/100 g and 3.52 mg quercetin/100 g, respectively. The extract of TP showed a potential antioxidant activity in the DPPH radical-scavenging assay ($EC_{50}=16.76{\mu}g/mL$). Pork patties were manufactured with 0.25% (T1), 0.5% (T2), 0.75% (T3) and 1.0% (T4) TP in a basic formula (C). The pH and volatile basic nitrogen (VBN) values of T2, T3 and T4 patties were lower (p<0.05) than the C patties during storage. Increased concentration of TP in meat patties decreased (p<0.05) the 2-thiobarbituric acid reactive substances (TBARS) and total plate count (TPC) values at d 7 of storage. Tomato treated-patties had lower (p<0.05) values for lightness ($L^*$), but higher (p<0.05) values for redness ($a^*$) and yellowness ($b^*$) at d 3 and 7 of storage compared with the C. In the case of sensory evaluation, the scores of colour, flavour and overall acceptability of T3 and T4 patties were higher (p<0.05) than those of the C patty after 3 or 7 days of storage.

• Korean Journal of Ecology and Environment
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• v.42 no.1
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• pp.9-18
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• 2009

The objectives of this study were to evaluate seasonal and interannual variations of water quality and nutrient input (N, P) in Junam Reservoir, a nesting waterbody of migratory birds, over 10 years during 1998$\sim$2007 along with dynamic relations of trophic parameters using empirical models. Concentrations of COD averaged 7.8 mg $L^{-1}$ during the study, while TN and TP were $1.4\;mg\;L^{-1}$ and $83{\mu}g\;L^{-1}$, respectively, indicating an eutrophic-hypereutrophic state. Values of monthly COD had strong positive relations (r=0.669, p<0.001) with conductivity, indicating that summer rainfall resulted in an ionic dilution of the reservoir water by rainwater and contributed better water quality. One-way ANOVA tests showed significant differences (F=$5.2{\sim}12.9$, p<0.05) in TN and TP between the before and after the bird migration. In other words, nutrient levels were greater in the absence of migratory birds than in the presence of the migratory birds, suggesting a no-effect on nutrient inputs by the birds. Also, one-way ANOVA indicated no significant differences (F=$0.37{\sim}0.48$, p>0.05) in $NO_{3^-}N$ and $NH_{3^-}N$ between the before and after the birds migration. Linear empirical models using trophic parameters showed that algal biomass as CHL, had significant low correlations with TN ($R^2$=0.143, p<0.001, n=119) and TP ($R^2$=0.192, p<0.001, n=119). These results suggest that influences of nutrients on the CHL were evident, but the effect was weak. This fact was supported by analysis of Trophic State Index Deviation (TSID). Over 70% in the observed values of "TSI (CHL)-TSI (SD)" and "TSI (CHL)-TSI (TP)" were less than zero, suggesting a light limitation on the CHL by inorganic suspended solids.

• IMMUNE NETWORK
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• v.3 no.2
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• pp.126-135
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• 2003

Background: One of the important factors in the prognosis of chronic hepatitis B patient is the degree of replication of hepatitis B virus (HBV). It has been known that HBV DNA polymerase plays the essential role in the replication of HBV. HBV DNA polymerase is composed of four domains, TP (Terminal protein), spacer, RT (Reverse transcriptase) and RNaseH. Among these domains, tyrosine, the $65^{th}$ residue of TP is an important residue in protein-priming reaction that initiates reverse transcription. If monoclonal antibody that recognizes around tyrosine residue were selected, it could be applied to further study of HBV replication. Methods: To produce TP-specific scFv (single-chain Fv) by phage display, mice were immunized using synthetic TP-peptide contains $57{\sim}80^{th}$ amino acid residues of TP domain. After isolation of mRNA of heavy-variable region ($V_H$) and light-chain variable region ($V_L$) from the spleen of the immunized mouse, DNA of $V_H$ and $V_L$ were obtained by RT-PCR and joined by a DNA linker encoding peptide (Gly4Ser)3 as a scFv DNA fragments. ScFv DNA fragments were cloned into a phagemid vector. scFv was expressed in E.coli TG1 as a fusion protein with E tag and phage gIII. To select the scFv that has specific affinity to TP-peptide from the phage-antibody library, we used two cycles of panning and colony lift assay. Results: The TP-peptide-specific scFv was isolated by selection process using TP-peptide as an antigen. Selected scFv had 30 kDa of protein size and its nucleotide sequences were analyzed. Indirect- and competitive-ELISA revealed that the selected scFv specifically recognized both TP-peptide and the HBV DNA polymerase. Conclusion: The scFv that recognizes the TP domain of the HBV DNA polymerase was isolated by phage display.

• Journal of Microbiology and Biotechnology
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• v.31 no.8
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• pp.1134-1143
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• 2021

In the present study, we investigated the inhibitory effect of heat-killed Enterococcus faecalis (E. faecalis) and live E. faecalis on benign prostatic hyperplasia (BPH). The BPH rat model was established by administering male rats with testosterone propionate (TP, 5 mg/kg, in corn oil) via subcutaneous injections daily for four weeks after castration. The rats were divided into five groups: Con, corn oil-injected (s.c.) + DW administration; BPH, TP (5 mg/kg, s.c.) + DW administration; BPH+K_EF, TP (5 mg/kg, s.c.) + heat-killed E. faecalis (7.5 × 10¹² CFU/g, 2.21 mg/kg) administration; BPH+L_EF, TP (5 mg/kg, s.c.) + live E. faecalis (1 × 10¹¹ CFU/g, 166 mg/kg) administration; BPH+Fi, TP (5 mg/kg, s.c.) + finasteride (1 mg/kg) administration. In both of BPH+K_EF and BPH+L_EF groups, the prostate weight decreased and histological changes due to TP treatment recovered to the level of the Con group. Both of these groups also showed regulation of androgen-signaling factors, growth factors, and apoptosis-related factors in prostate tissue. E. faecalis exhibited an inhibitory effect on benign prostatic hyperplasia, and even heat-killed E. faecalis showed similar efficacy on the live cells in the BPH rat model. As the first investigation into the effect of heat-killed and live E. faecalis on BPH, our study suggests that heat-killed E. faecalis might be a food additive candidate for use in various foods, regardless of heat processing.

• Journal of the Korean Society of Tobacco Science
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• v.23 no.2
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• pp.109-114
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• 2001

A CAB cDNA vector(pKGCAB), encoding the light harvesting chlorophyll a/b binding protein in Korean ginseng (Panax ginseng C. A. Meyer), was constructed with the CaMV35S promoter of plant expression vector. The chimeric vector was transformed into tobacco(Nicotiana tabacum cv. NC 82) using Agrobacterium tumefaciens LBA 4404 strain, and the transgenic tobacco plant CAB-TP2 was selected. Photosynthetic rates of the CAB-TP2 plant at before-flowering stage were increased about 20% under low irradiance conditions of quantum 100 and 500 $\mu$mol.m$^{-2}$ s$^{-1}$ , however, the rates were similar to those of NC 82 under quantum 1000 and 2000 $\mu$mol.m$^{-2}$ s$^{-1}$ conditions. The plants were germinating under low- or normal irradiance condition and the quantum yield of photosystem III were measured. The differences of the Fv/Em values between conditions were 0.07 and 0.01 in NC 82 and CAB-TP2, respectively. The mature leaves in the position 8-10 of the CAB-TP2 at before-flowering stage revealed l0% higher Fv/Fm values in range of 0.759 to 0.781 and 40% more chlorophyll contents of 70-93mg/$m\ell$ than those of normal NC 82. These data suggest the possibility that the increase in photosynthetic activity of leaves under low light intensity in the canopy of CAB-TP2 transgenic tobacco might lead to increase the quality of lower tobacco leaves.

• The Korean Journal of Food And Nutrition
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• v.25 no.4
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• pp.807-819
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• 2012

To evaluate the functional characteristic and availability for drinking of the fermented Smilax china leaf tea by using different microbial species, various fermented leaf tea was prepared by non-fermentation (C), or the fermentation of Saccharomyces cerevisiae (S), Bacillus sp. (B), Bifidobacterium bifidus (L), Monascus pilosus (M) and Aspergilus oryzae (A), and sensory and antioxidant parameter of each brewed tea was observed. The color of the A tea was red, but the other teas were yellow in color. Furthermore, the aesthetic quality of the A and M tea was 3.95 and 3.30 point, respectively, and other teas (2.55~2.28) were similar to that of the C tea. TP of fermented tea water extract was lower than that of the C, although TF was not significantly different between the fermented and non-fermented tea. Especially, TF of the A tea was significantly lower than those of the other teas. The range of EDA ($1mg/m{\ell}$) of water and ethanol extracts of tea C and the fermented teas was 19.25~22.48%; however, tea A was only 8.04~12.49%. In addition, FRAP, FICA and LPOIA of teas were not significantly different between the fermented and non-fermented teas. On the other hand, XOIA and AOIA of tea ethanol extracts were slightly higher than those of water extracts. XOIA of water extract derived from the teas was 4.83~9.20%, while ethanol extract of these was 9.00~19.00%. However, XOIA of B and L teas water extract was not detected. Furthermore, AOIA of fermented tea water extract (30.17~48.52%) were lower than those of ethanol extract (44.09~66.93%). In this study, interestingly, antioxidant parameters, such as FRAP, FICA, LPOIA and AOIA, of the A tea water extract (0.1%) was higher than that of the other tea in spite of high decreasing rate in the contents of TP and TF. Therefore, above results imply the possibility of fermented Smilax china leaf tea as a functional food.

• Journal of Korean Society of Environmental Engineers
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• v.27 no.11
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• pp.1162-1167
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• 2005

Livestock wastewater is known to be very difficult to treat because it contains highly non-biodegradable organic material. Thus the DOF(Dissolved Ozone Flotation) system and the PO2(Pressurized Ozone Oxidation) system were built at the livestock wastewater treatment plant, and characteristics of treatments were investigated in this paper. Suspended Solids(SS) removal efficiency was over 94% by DOF system. 90% of $COD_{Mn}$ was removed, from 620 mg/L down to 63 mg/L by the DOF-PO2 system. During the period of operation, $SCOD_{Cr}$ was removed an average of 82%, from 890 mg/L down to 160 mg/L. 96% of UV-254 absorbance was also removed. TP removal efficiency was over 98%, from 27 mg/L to 0.35 mg/L, and TN was also removed 68% along with suspended solids. It was possible to meet effluent standards of the livestock wastewater treatment plant by the DOF-PO2 system along with biological treatment.