• 한국수정란이식학회지
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• 제15권3호
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• pp.247-253
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• 2000

This study was undertaken to optimize enucleation and reconstitution methods for the production of cloned mice by somatic cell nuclear transfer Outbred ICR mouse oocytes at the metapahse- II stage were retrieved from female mice superovulated by PMSG and hCG. In Experiment 1, oocytes were enucleated in medium supplemented with cytochalasin B (CCB) of 3 levels (0, 7.5 or 15 $\mu\textrm{g}$/mL), and higher rate of encleation was obtained at 7.5 and 15 $\mu\textrm{g}$/mL than at $\mu\textrm{g}$/mL. In Experiment 2, oocytes enucleated in 7.5 $\mu\textrm{g}$/mL CCB-containing medium were reconstituted with different types of somatic cell by following methods; 1) cumulus cells by direct cell injection, 2) cumulus cells by electric fusion (1.25 kV/cm, 2 pulses for each 70 $mutextrm{s}$) or 3) STO cells by the electrofusion. Electrofusion of STO cells with enucleated oocytes yielded the greatest (P<0.05) rate of reconstitution without lysis (76%) than any other combinations. Although significant decrease in the rate of somatic cell introduction was found, the electrofusion of cumulus cells yielded better rate of reconstitution than direct injection (0 vs. 18%). In Experiment 3, the duration of electric stimulation for the fusion was changed to either 50 $mutextrm{s}$ or 90 $mutextrm{s}$, but no significant improvement of reconstitution efficacy was obtained. In conclusion, this study showed that ICR mouse oocytes could be used for the production of reconstituted oocytes and a fusion method of 1.25 KV/cm with 2 pulses using 570 cell was the optimal.

• 비파괴검사학회지
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• 제33권2호
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• pp.212-218
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• 2013

A transfer method of neutron radiography was developed to measure the size of the end plug and a gap of an intact K102L-2, the irradiated fuel of a ballooned K174L-3, a ballooned and ruptured K98L-3. A typical irradiation time of 25 min. was determined to obtain a film density of between 2 and 3 of SR X-ray film with neutrons of $1.5{\times}10^{11}n{\cdot}cm^{-2}$. To validate and calibrate the results, a RISO fuel standard sample, Cd plate and ASTM-BPI/SI were used. An activated latent image formed in the $100{\mu}m$ Dy foil was subsequently transferred in a dark room for more than 8 hours to the SR film which is a maximum of three half-lives. Due to the L/D ratio an unsharpness of $9.82-14{\mu}m$ and a magnification of 1.0003 were given. After digitizing an image of SR film, the ballooning gap of the plug was discernible by an H/V filter of image processing. The gap size of the ballooned element, K174L-3, is equal to or greater than 1.2 mm. The development of a transfer method played a pivotal role in developing high burn-up of Wolsung and PWR nuclear fuel type.

• Environmental Engineering Research
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• 제19권4호
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• pp.345-354
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• 2014

In this paper, effects of physical configurations and operating conditions on bubble column performance were analyzed in terms of bubble hydrodynamic and mass transfer parameters. Bubble column with 3 different dimensions and 7 gas diffusers (single / multiple orifice and rigid / flexible orifice) were applied. High speed camera and image analysis program were used for analyzing the bubble hydrodynamic parameters. The local liquid-side mass transfer coefficient ($k_L$) was estimated from the volumetric mass transfer coefficient ($k_La$) and the interfacial area (a), which was deduced from the bubble diameter ($D_B$) and the terminal bubble rising velocity ($U_B$). The result showed that the values of kLa and a increased with the superficial gas velocity (Vg) and the size of bubble column. Influences of gas diffuser physical property (orifice size, thickness and orifice number) can be proven on the generated bubble size and the mass transfer performance in bubble column. Concerning the variation of $k_L$ coefficients with bubble size, 3 zones (Zone A, B and C) can be observed. For Zone A and Zone C, a good agreement between the experimental and the predicted $K_L$ coefficients was obtained (average difference of ${\pm}15%$), whereas the inaccuracy result (of ${\pm}40%$) was found in Zone B. To enhance the high $k_La$ coefficient and absorption efficiency in bubble column, it was unnecessary to generate numerous fine bubbles at high superficial gas velocity since it causes high power consumption with the great decrease of $k_L$ coefficients.

• 한국정밀공학회지
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• 제29권1호
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• pp.114-120
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• 2012

We present a simple and low-cost method to fabricate poly(methyl-methacrylate) (PMMA) nanochannels with various shapes by combining the standard optical lithography with a PMMA layer transfer and collapse technique. We utilized PMMA membrane reflowing/collapsing phenomena into microchannels to fabricate nanochannels at both corners of arbitrarily-shaped microchannels. This allows nanochannels with various shapes such as curved nanochannels as well as straight nanochannels to be easily fabricated since the shape of the microchannel determines the shape of the nanochannels. This nanochannel fabrication method is simple, flexible, and low-cost since the standard optical lithography with low-resolution optical masks can be used to fabricate nanoscale channels as small as 100 nm wide with various shapes. Also, the sealing of nanochannels can be naturally achieved while the nanochannels are formed through the polymer layer transfer and collapse.

• 한국수정란이식학회지
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• 제11권2호
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• pp.95-101
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• 1996

The present study investigated the effect of treatment with nocodazole on the efficiency of cell cycle synchronization and development of mouse 4-cefl embryos. In addition, developmental ability of reconstituted embryos that received nuclei from 4-cell embryos synchronized with nocodazole at M phase was examined in vitro and in vivo. (1) When 4-cell blastorneres exposed to culture medium containing l$\mu$g /ml nocodazole for 2, 4, 6 and 8 hrs, 40% (40/10l), 75% (l19/159), 85% (87/102) and 97% (155/160) of nuclei were synchronized at M phase, respectively. (2) Treated with nocodazole for 4 hrs, the proportion of 4-cell embryos developed to blastocysts (98%, 60/61) was not significanUy different from that of the control embryos (98%, 196/201). However, the developmental ability of 4-cell embryos treated for 8 (87%, P<0.05)and 12 hrs (76%, P

• 설비공학논문집
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• 제11권4호
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• pp.472-483
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• 1999

The evaporation heat transfer and pressure drop characteristics for HFC-l34a in flat plate type heat exchangers with enhanced beads were experimentally investigated. Three plate type evaporators with different geometric condition of U-turn area were tested. Mass fluxes were tested over the range of 83kg/$m^2$s to 166kg/$m^2$s, and heat fluxes were varied from 4㎾/$m^2$ to 12㎾/$m^2$. Evaporation temperature was 5$^{\circ}C$ with inlet qualities of 0.1 to 1.0. There was no notable difference in the heat transfer coefficient by geometric variation of U-turn area, but the third plate with cross-ribbed channel at U-turn area was better than others in the evaluation using volume goodness factor comparison. Also, the mixtures of HFC-l34a and PAG oil was tested to determine oil effects on heat transfer and pressure drop. As oil concentration was increased, heat transfer coefficient was increased by 22~48% up to the 3wt.%, but decreased by 14~22% at the 4wt.%. The pressure drop was increased by the maximum of 100% as oil concentration was increased.

• 말소리와 음성과학
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• 제3권3호
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• pp.77-88
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• 2011

Foreign accent in second language production depends heavily on the transfer of features from the first language. This study examines acoustic variations in segments and suprasegments by native and nonnative speakers of English, searching for patterns of the transfer and plausible indexes of foreign accent in English. The acoustic variations are analyzed with recorded read speech by 20 native English speakers and 50 Korean learners of English, in terms of vowel formants, vowel duration, and syllabic variation induced by stress. The results show that the acoustic measurements of vowel formants and vowel and syllable durations display difference between native speakers and nonnative speakers. The difference is robust in the production of lax vowels, diphthongs, and stressed syllables, namely the English-specific features. L1 transfer on L2 specification is found both at the segmental levels and at the suprasegmental levels. The transfer levels measured as groups and individuals further show a continuum of divergence from the native-like target. Overall, the eldest group, students who are in the graduate schools, shows more native-like patterns, suggesting weaker foreign accent in English, whereas the high school students tend to involve larger deviation from the native speakers' patterns. Individual results show interdependence between segmental transfer and prosodic transfer, and correlation with self-reported proficiency levels. Additionally, experience factors in English such as length of English study and length of residence in English speaking countries are further discussed as factors to explain the acoustic variation.

• 대한기계학회논문집B
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• 제39권9호
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• pp.719-726
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• 2015

전열량을 늘리는 손쉬운 방법은 전열면적을 크게 하는 것이다. 본 연구에서는 세로와 가로 방향 튜브 핏치의 비(Pt/Pl)가 1.03인 광폭 루버 핀 시료의 습표면 j와 f 인자를 실험을 통하여 구하고 Pt/Pl=0.6인 일반 루버 핀 시료와 비교하였다. 동일 소비동력에서 광폭 루버 핀 시료의 전열성능이 일반 루버 핀 시료보다 1열에서 평균 16%, 2열에서 평균 29%, 3열에서 평균 38% 크게 나타났다. 이 증가량은 핀 면적의 증가량 (2.17배)에 비하면 현저히 작은데 이는 광폭 루버 핀 시료의 열전달계수와 핀 효율이 일반 루버 핀 시료의 값들보다 작기 때문이다. 핀 핏치가 j와 f 인자에 미치는 영향이 크지 않았다. 또한 튜브 열수가 증가할수록 j와 f 인자는 감소하였다. 실험 데이터를 기존 상관식과 비교하였다.

• 대한기계학회논문집B
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• 제39권1호
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• pp.61-69
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• 2015

본 논문은 수직 오리피스 이젝터의 혼합유동 및 산소전달 특성에 대한 실험적 연구를 목적으로 한다. 실험장치는 전동 모터-펌프, 오리피스 이젝터, 순환 수조, 공기압축기, 고속 카메라 시스템 그리고 제어 및 측정기기로 구성하였다. 측정된 구동유체 및 유입공기의 유량을 이용하여 유량비를 도출하였다. 이적터에서 분출된 혼합유동의 가시화를 통해 정성적 거동을 고찰하였으며, 용존산소 농도를 측정하여 총괄 산소전달계수를 도출하였다. 구동유체의 유량이 일정하고 압축기의 공기압이 높아지면 유량비와 산소전달계수는 증가하며, 압축기의 공기압이 일정하고 구동유체의 유량이 증가하면 유량비는 감소하지만 산소전달계수는 증가하였다. 기포의 크기에 따른 체류시간 및 확산도와 수직 혼합유동의 도달거리는 2 상의 접촉면적과 시간에 크게 영향을 미치기 때문에 산소전달율의 중요한 변수임을 유추할 수 있다.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2007년도 춘계학술대회B
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• pp.2817-2822
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• 2007

The purpose of this paper is to study the influence of operating condition and ejector geometries on the hydrodynamics and on the mass transfer characteristic of ejector. The CFD results were validated with available experimental data. Flow field analyses and predictions of ejector performance were also carried out. Variation on the operating conditions was made by varying the gas-liquid flow rate ratio in the range of 0.2 to 1.2. The ejector configuration was also varied on the length to diameter ratio of mixing tube ($L_M/D_M$) in the range of 4 to 10. CFD studies show that at $L_M/D_M$ 5.5, the volumetric mass transfer coefficient increases with respect to gas flow rates. Meanwhile, at $L_M/D_M$ 4, the plot of volumetric mass transfer coefficient to gas-liquid flow rates ratio reach maximum at gas-liquid flow rates ratio of 0.6. This study also shows that volumetric mass transfer coefficient decrease with respect to the increase of mixing tube length.