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연초(Nicotiana tabacum cv. Xanthi) 배양세포로부터 Ubiquinone-10 생산을 위한 현탁배양

  • 양덕춘;최광태;박지창;강신웅;이정명
    • Journal of the Korean Society of Tobacco Science
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    • v.21 no.1
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    • pp.64-69
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    • 1999
  • The effect of phytohormones, light and phosphate on in vitro production of ubiquinone 10 from the suspension cultures of Nicotiana tabacum cv. Xanthi callus was investigated. The inoculum size and cultured time in the suspension culture had to be at least over 2 % of medium volume at 15 days for the excellent growth of Xanthi callus. The growth of Xanthi callus in the suspension culture was improved by addition of NAA and 2,4-D, especially NAA 1.0mg/1 alone, at the light condition. The optimal concentration of phytohormone was 0.1 mg/l 2.4-D and 1.0 mg/l NAA for productivity of ubiquinone 10 in the suspenseion of Xanthi callus. Addition of 3mM KH$_2$PO$_4$ to the medium was more effective in promoting ubiquinone-l0 formation than other concentration in the light condition. Content and production of ubiquinone-l0 in the suspension cultures of Xanthi callus were the highest at the MS media containing 0.5mg/L kinetin, 0.5mg/L 2,4-D, 1.0mg/1 NAA, 3mM phosphate and 2 % inoculum in the light.

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Design of a Compact LPDA Antenna using Inverted-L Shaped Dipole Elements (Inverted-L 형태의 다이폴 소자를 이용한 소형 LPDA 안테나 설계)

  • Yeo, Jun-Ho;Lee, Jong-Ig
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.36 no.7A
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    • pp.678-682
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    • 2011
  • In this paper, a compact log periodic dipole array (LPDA) antenna operating from 1 to 6 GHz is studied. Inverted-L shaped dipole elements are used to miniaturize the lateral size of an LPDA antenna and the spacing factor is also decreased to reduce the total length of the LPDA antenna. As the top-loading length of the inverted-L shaped dipole elements is increased, the width of the LPDA antenna is decreased but the bandwidth and the gain of the antenna are decreased. The fabricated compact LPDA antenna is printed on FR4 substrate With a dielectric constant of 4.4 and a thickness of 1.6 mm, and its size is reduced to 32% in width and 49% in length compared to a standard LPDA antenna.

Somatic Embryogenesis and Plant Regeneration from Immature Zygotic Embryo Culture of Wasabia japonica Matsum. (고추냉이의 미숙배배양으로부터 체세포배 발생과 식물체 재분화)

  • 은종선;고정애;김영선;김명준
    • Korean Journal of Plant Tissue Culture
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    • v.22 no.4
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    • pp.207-211
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    • 1995
  • Immature zygotic embryos from immature seeds of Wasabia japonica (cv Dalma) were isolated and cultured on modified MS medium supplemented with 2,4-D, IAA, and BA. Immature zygotic embryos were classified into torpedo shape and cotyledon stage. The highest rates of callus formation were obtained of 1.0mg/L IAA(torpedo stage, 90.0%)and 1.0mg/L 2,4D plus 0.1mg/L BA(cotyledany stage,84.3%). Somatic embryos after 60 days of culture. These numerous somatic embryo could be seperated and subcultured on the same media for further propagation. After 90 days of culture, most somatic embryos were developed well organized embryos which were able to produce into whole plants.

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Synthesis, Characterization and in vitro Antibacterial Studies on Mixed Ligand Complexes of Iron(III) Based on 1,10-phenanthroline

  • Tigineh, Getinet Tamiru;Sitotaw, Getu;Workie, Amogne;Abebe, Atakilt
    • Journal of the Korean Chemical Society
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    • v.65 no.3
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    • pp.203-208
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    • 2021
  • As part of our attempt to discover novel active compounds against multi-drug resistant pathogens, we hereby report two new complexes of iron(III) with formulae: [Fe(L1)2(H2O)2]Cl3 and [Fe(L1)2(L2)(H2O)]Cl2 where L1 = 1,10-phenanthroline (C12H8N2) and L2 = guanide (C5H4N5O-). The synthesized complexes were characterized using spectroscopic analysis (ESI-MS, ICP-OES, FT-IR, and UV-Vis), cyclic voltammetry, CHN analysis, gravimetric chloride determination, melting point determination, and conductance measurement. Octahedral geometries are assigned to both complexes. In vitro antibacterial activity was tested on two Gram-positive (Staphylococcus aureus, Streptococcus epidermidis) and two Gram-negative (Escherichia coli and Klebsiella pneumoniae) bacteria using the disc diffusion method. The complexes demonstrated appreciable activity against these pathogens. Interestingly, the [Fe(L1)2(L2)(H2O)]Cl2 complex manifested a higher degree of inhibition against the drug-resistant Gram-negative bacteria than the commercially available drug, namely erythromycin.

Derivation of Optimal Distribution for the Frequency Analysis of Extreme Flood using LH-Moments (LH-모멘트에 의한 극치홍수량의 빈도분석을 위한 적정분포형 유도)

  • Maeng, Sung-Jin;Lee, Soon-Hyuk
    • Proceedings of the Korean Society of Agricultural Engineers Conference
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    • 2002.10a
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    • pp.229-232
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    • 2002
  • This study was conducted to estimate the design flood by the determination of best fitting order of LH-moments of the annual maximum series at six and nine watersheds in Korea and Australia, respectively. Adequacy for flood flow data was confirmed by the tests of independence, homogeneity, and outliers. Gumbel (GUM), Generalized Extreme Value (GEV), Generalized Pareto (GPA), and Generalized Logistic (GLO) distributions were applied to get the best fitting frequency distribution for flood flow data. Theoretical bases of L, L1, L2, L3 and L4-moments were derived to estimate the parameters of 4 distributions. L, L1, L2, L3 and L4-moment ratio diagrams (LH-moments ratio diagram) were developed in this study.

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Oxidative Modification of Neurofilament-L Induced by Endogenous Neurotoxin, Salsolinol

  • Kang, Jung-Hoon
    • Bulletin of the Korean Chemical Society
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    • v.32 no.9
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    • pp.3421-3424
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    • 2011
  • The endogenous neurotoxin, 1-methyl-6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline (salsolinol), has been considered a potential causative factor for the pathogenesis of Parkinson's disease (PD). In this study, we examined oxidative modification of neurofilament-L (NF-L) induced by salsolinol. When disassembled NF-L was incubated with salsolinol, the aggregation of protein was increased with the concentration of sasolinol. The formation of carbonyl compound was obtained in salsolinol-mediated NF-L aggregates. This process was protected by free radical scavengers, such as N-acetyl-L-cysteine and glutathione. These results suggest that the aggregation of NF-L is mediated by salsolinol via the generation of free radicals. We also investigated the effects of copper ion on salsolinol-mediated NF-L modification. In the presence of copper ions, salsolinol enhanced the modification of NF-L. We suggest that salsolinol might be related to abnormal aggregation of NF-L which may be involved in the pathogenesis of neurodegenerative diseases and related disorders.

Estimation of Design Flood by the Determination of Best Fitting Order of LH-Moments ( I ) (LH-모멘트의 적정 차수 결정에 의한 설계홍수량 추정 ( I ))

  • 맹승진;이순혁
    • Magazine of the Korean Society of Agricultural Engineers
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    • v.44 no.6
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    • pp.49-60
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    • 2002
  • This study was conducted to estimate the design flood by the determination of best fitting order of LH-moments of the annual maximum series at six and nine watersheds in Korea and Australia, respectively. Adequacy for flood flow data was confirmed by the tests of independence, homogeneity, and outliers. Gumbel (GUM), Generalized Extreme Value (GEV), Generalized Pareto (GPA), and Generalized Logistic (GLO) distributions were applied to get the best fitting frequency distribution for flood flow data. Theoretical bases of L, L1, L2, L3 and L4-moments were derived to estimate the parameters of 4 distributions. L, L1, L2, L3 and L4-moment ratio diagrams (LH-moments ratio diagram) were developed in this study. GEV distribution for the flood flow data of the applied watersheds was confirmed as the best one among others by the LH-moments ratio diagram and Kolmogorov-Smirnov test. Best fitting order of LH-moments will be derived by the confidence analysis of estimated design flood in the second report of this study.

Syntheses and Theoretical Study of Palladium(II) Complexes with Aminophosphines as 7-Membered Chelate Rings

  • 김봉곤;양기열;정맹준;이배욱;도명기
    • Bulletin of the Korean Chemical Society
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    • v.18 no.11
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    • pp.1162-1166
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    • 1997
  • Nature of palladium(Ⅱ) complexes with 7-membered chelates was studied by experimental and theoretical methods on a Pd(L)Cl2 system, where L is Ph2PNHCH2CH2NHPPh2(L1), Ph2PNHC6H4NHPPh2(L2). The palladium(Ⅱ) complexes were prepared and characterized by elemental analysis, IR, UV, 1H, and 31P NMR spectroscopy. Ab initio calculations with geometry optimizations were also performed for related model systems, Pd(L)Cl2; L=R2PNH(CH2)2NHPR2(L3), R2PNHC6H4NHPR2(L4), R2P(CH2)4PR2(L5), R2PCH2(C6H4)CH2PR2(L6); R=H, CH3.

Effects of Dietary L-leucine Levels in Low-lysine Diets on Growth Performance and Meat Quality Parameters in Finishing Duroc Pigs (저수준의 Lysine 사료에서 L-leucine 첨가수준이 비육후기 Duroc 품종 돼지의 성장 및 육질특성에 미치는 영향)

  • 박준철;김영화;정현정;이성대;조규호;김인철;이상진;문홍길
    • Journal of Animal Science and Technology
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    • v.48 no.6
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    • pp.813-818
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    • 2006
  • The objective of this study was to investigate effects of supplementation levels of L-leucine in low-lysine diets on growth performance and meat quality parameters in finishing Duroc pigs. A total of fifty-four pigs with an average initial weight of 74±1kg were allotted to one of three dietary treatments. Each treatment had six replications of three pigs per replicate. The treatment diets included 1)a corn-soybean meal basal diet containing lysine as low as 0.45% (CON), 2) basal diet plus 1.5% L-leucine (LEU 1.5), and 3) basal diet plus 3.0% L-leucine (LEU 3.0). No difference was found in ADG, ADFI and Feed/Gain among treatments. In carcass parameters, dressing precent and back-fat thickness were not affected by L-leucine levels, however, longissimus dorsi area tended to increase by supplementation of L-leucine. Marbling score was significantly higher (P<0.05) in LEU 1.5 and LEU 3.0 than in CON. In meat quality parameters, CIE L* of meat color significantly increased (P<0.05) and crude fat tended to increase by supplementation of L-leucine. However, there was no difference in water holding capacity, cooking loss and shear force value. This study suggests that dietary supplementation of L-leucine in low-lysine diets at the end of finishing period can produce high marbled pork which is preferred by Korean consumers.

Combined Effects of Hypoxia and Hydrogen Sulfide on Survival, Feeding Activity and Metabolic Rate of Blue crab, Portunus trituberculatus (꽃게, Portunus trituberculatus의 생존, 섭이활동 및 대사률에 미치는 빈산소와 황화수소의 복합적 영향)

  • KANG Ju-Chan;MATSUDA Osamu;CHIN Pyung
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.28 no.5
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    • pp.549-556
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    • 1995
  • Experiments were carried out to examine the combined effects of hypoxia and hydrogen sulfide on survival, feeding activity and metabolic rata or Blue crab, Portunus trituberculatus. Survival rate of the crab was significantly affected by $\leq2.14mg/l$ dissolved oxygen, and feeding activity was also reduced below 1.41mg/l dissolved oxygen. Metabolic rate of the crab exosed to hypoxia $(\leq3.35mg/l)$ was significantly reduced than that exposed to normoxia. The combined effects of hypoxia $(\leq1.86mg/l)$ and hydrogen sulfide $(12.35 {\mu}g/l)$ on the survival rate were highly toxic than each single effect. Feeding activity was also decreased by the combinedexposure to $\leq1.86mg/l$ dissolved oxygen and $(12.35 {\mu}g/l)$ hydrogen sulfide compared with single effect.

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